Q13MI3 (METE_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bxeno_B1738
ORF Names:	Bxe_B1250

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000071609
Sites
	Metal binding	641	1	Zinc By similarity
	Metal binding	643	1	Zinc By similarity
	Metal binding	726	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13MI3 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 760165E87B221D61	FASTA	764	84,949
	10 20 30 40 50 60 MARTHIPGFP RIGAQRELKF AQESFWRGES GEPHLRGVAR ELRARHWQLQ QDAGLDFVTV 70 80 90 100 110 120 GDFAYYDQML NLSALLGALP QRFGFEPKTL SLAEYYELAR GNAAQPAMEM TKWFDTNYHY 130 140 150 160 170 180 LVPELGPQTS FDGGVEWLFE EIDEALALNL PVKPVLIGPI TYLWLSKSHV AGFDRLSLLP 190 200 210 220 230 240 KLVIRYARLL DKLRQSGIEW VQLDEPALCV DLPAEWLDAF SAAYEVLGTS GVKVLLATYF 250 260 270 280 290 300 ESAAEHAPRV AALPVAGVHL DLVRAPQQLD AWRAALPAHA VLSAGVIDGR NIWRADLGEI 310 320 330 340 350 360 VESLQSLQAE FGERLWIAPS CSLLHVPVSL DAEKKLDADL KSWLAFATEK LAEVATLALA 370 380 390 400 410 420 LRDPAAAEPA LAATDRALEA RRRSSAVVNA LVQKRVAAVS SAMAERQSPF AERNRLQREA 430 440 450 460 470 480 LGLPLLPTTT IGSFPQTAAI RQARAAYKRG ELRALDYLQR MRTEIEIAVR KQEELGLDVL 490 500 510 520 530 540 VHGEAERNDM VEYFGEQLWG YAFTENGWVQ SYGSRCVKPP IIYGDVYRPE PMTVETTRYA 550 560 570 580 590 600 QSLTQRLMKG MLTGPVTMLQ WSFVRDDQPR STTALQLALA IRDEVVDLEK AGIRIIQIDE 610 620 630 640 650 660 PAFREGLPLR RADWAAYLEW ATRAFRISAA GVADQTQIHT HMCYSEFNDI LPSIAAMDAD 670 680 690 700 710 720 VITIETSRSA MELLDGFGAF AYPNEIGPGV YDIHSPRVPG ADAMQRLLER ACEVIPAERL 730 740 750 760 WVNPDCGLKT RGWPETEAAL ANMVRAAKAL RAKLAARQAD ELTA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000271 Genomic DNA. Translation: ABE34706.1.
RefSeq	YP_554056.1. NC_007952.1.
3D structure databases
ProteinModelPortal	Q13MI3.
SMR	Q13MI3. Positions 3-753.
ModBase	Search...
Protein-protein interaction databases
STRING	Q13MI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4008261.
GenomeReviews	Gene locus Bxeno_B1738 in contig CP000271_GR.
KEGG	bxe:Bxe_B1250.
PATRIC	19338493. VBIBurXen52548_6505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	LWLSKSH.
PhylomeDB	Q13MI3.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BXEN266265:BXE_B1250-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURXL

Accession

Primary (citable) accession number: Q13MI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program