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Q13I15 (NAPA_BURXL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Bxeno_C0346
ORF Names:Bxe_C0367
OrganismBurkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]
Taxonomic identifier266265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 827795Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000256070

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q13I15 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 3B9D6FE05EE4C769

FASTA82792,597
        10         20         30         40         50         60 
MTLSRRAFIK QTAAATAASA AGVVLPGVDA LAASDSLTWS KAPCRFCGTG CGVSVGVKNG 

        70         80         90        100        110        120 
KVVATQGDPQ AEVNRGLNCV KGYFLSKIMY GQDRLTTPLL RMKDGKYAKD GEFAPVSWDQ 

       130        140        150        160        170        180 
AFDVMADHFK RTLKEKGPTA VGMFGSGQWT VWEGYAAVKL MKAGFRSNNL DPNARHCMAS 

       190        200        210        220        230        240 
AVTGFMRTFG MDEPMGCYDD IEQADTFVLW GSNMSEMHPI LWTRITDRRL STPTTRVVVL 

       250        260        270        280        290        300 
STFEHRSFDL ADQTIIFTPQ SDLAILNYIA NYIIRNGNVN RDFVNRHTVF KQGNADIGYG 

       310        320        330        340        350        360 
LRPDNPLQKT ARNAGDPNGS QPITFDEFAK FVSKYDAAYV TKLSGVPQNK LDQLARLYAD 

       370        380        390        400        410        420 
PKVKVMSFWT MGFNQHTRGT WANNMVYNLH LLTGKIATPG NSPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFSHRLPADM VVTNPKHREE AEHIWKLPAG TIPDKPGYHA VLQNRMLRDG KLNAYWVQVN 

       490        500        510        520        530        540 
NNVQAAANIN GEALPGYRNP QAFVVVSDVY PTVTAVAADL ILPSAMWVEK EGAYGNAERR 

       550        560        570        580        590        600 
TQFWHQLVDA PAGARSDLWQ LVEFSKRFKV EEVWPADLLA KKPEYRGKTL YDVLYRNGQV 

       610        620        630        640        650        660 
DRFALTETDS HYRNDEAKAF GFYIQKGLFE EYASFGRGHG HDLAPFDAYH KARGLRWPVV 

       670        680        690        700        710        720 
NGKETRWRYK EGSDPYVKTG TGWQFYGNPD GRAVIYALPY EPPPEVPDKE YPFWLATGRV 

       730        740        750        760        770        780 
LEHWHSGSMT RRVPELYRAF PNAVCFMHPD DAKAMGVRRG VEVKVMSRRG YILTRVETRG 

       790        800        810        820 
RDKPPRGLVF VPWFDSSQLI NKVTLDATDP ISLQTDYKKC AVKIVKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000272 Genomic DNA. Translation: ABE36274.1.
RefSeqYP_555624.1. NC_007953.1.

3D structure databases

ProteinModelPortalQ13I15.
SMRQ13I15. Positions 37-824.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266265.Bxe_C0367.

Proteomic databases

PRIDEQ13I15.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE36274; ABE36274; Bxe_C0367.
GeneID4009881.
KEGGbxe:Bxe_C0367.
PATRIC19341756. VBIBurXen52548_8136.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFHAVLQD.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycBXEN266265:GJII-8021-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_BURXL
AccessionPrimary (citable) accession number: Q13I15
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: August 22, 2006
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families