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Q13EL3 (TRMB_RHOPS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:RPD_0236
OrganismRhodopseudomonas palustris (strain BisB5) [Complete proteome] [HAMAP]
Taxonomic identifier316057 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288215

Sites

Active site1591 By similarity
Binding site851S-adenosyl-L-methionine By similarity
Binding site1101S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1591S-adenosyl-L-methionine By similarity
Binding site1631Substrate By similarity
Binding site1951Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q13EL3 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: 94486706E0E2607E

FASTA25629,350
        10         20         30         40         50         60 
MITIDRRVQT KCSVLGRHKQ VFIDMNETVH HQGSFFGRRK GHKLRAHQAD LVDNLLPHLA 

        70         80         90        100        110        120 
LDIELPAPDA LAELFDAPVE AVRLEIGFGG GEHLIAEALA HPEIGFIGAE PYVNGMAKIL 

       130        140        150        160        170        180 
ARIEDQNIRN VRLFAGDAAE LMAWLPARAL KRIDLIHPDP WPKRRHWKRR FVQDSMIGAM 

       190        200        210        220        230        240 
ARALIDGGEF RFVSDIDSYN AWTLAHLLRA PEFDWTAERA DDWRKPWSGY TMTRYGRKAE 

       250 
REGRRASYLR FRRIAA 

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris BisB5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Lykidis A., Oda Y., Harwood C.S., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BisB5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000283 Genomic DNA. Translation: ABE37476.1.
RefSeqYP_567377.1. NC_007958.1.

3D structure databases

ProteinModelPortalQ13EL3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316057.RPD_0236.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE37476; ABE37476; RPD_0236.
GeneID4020694.
KEGGrpd:RPD_0236.
PATRIC23275289. VBIRhoPal120395_0241.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMARAFYGRR.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycRPAL316057:GHDC-240-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RHOPS
AccessionPrimary (citable) accession number: Q13EL3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 31, 2006
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways