Reviewed, UniProtKB/Swiss-Prot Q13E74 (ACEK_RHOPS)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	RPD_0377

Organism

Rhodopseudomonas palustris (strain BisB5) [Complete proteome] [HAMAP]

Taxonomic identifier

316057 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288299
Regions
	Nucleotide binding	354 – 360	7	ATP By similarity
Sites
	Active site	414	1	By similarity
	Binding site	375	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q13E74-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 3684B16E8958EA11	FASTA	606	67,370
	10 20 30 40 50 60 MTADAHLFSA SDLEAATRIA EPDFELLDAL IRTDDPDDQA HTLARVALSA FDNYYAVSRR 70 80 90 100 110 120 IPALAKAAFY ARDWPATVRL SKIRIGLYTA CIDQLVPLLK AGLPELANDE QVWVRAEAEL 130 140 150 160 170 180 LAAIAGRYEA DFAFAFWQSL RRKLVSDEWR PVSYDTGPAA RPPAAIAAVV RTIAATLPIQ 190 200 210 220 230 240 PEVIRNVLDA AGFTGPWRDL DGDAALAAAA IEAALEPLSP RAGETAKIEI AESGFFRNRG 250 260 270 280 290 300 ACLVGRIRLR DRGDMPPRNI PLLVALLNED DGLVVDAVLC DSDELQFAFS STLANYHATN 310 320 330 340 350 360 PRYHELARLL HELMPKRPLG TQYSCIGFHH LGKVAVMNEI LTEHRRSKEK LATAPGFKGT 370 380 390 400 410 420 VAIAFTMPCS AYVLKIIRDH PTDDYKFDYF DGLDAVLRKY NLVHEIDRAG SMLDNIIYSN 430 440 450 460 470 480 VKLARTMFAP DLLDELLEAG IGTVTLERDA LVFRHLIVQI KLTPLPLYLT TAAAADARAA 490 500 510 520 530 540 VINLGDCIKN NAAADIFNKD LDGRNYGVSR IRKVYLFDYD AVEQLTEVKV RSTPPMPRAE 550 560 570 580 590 600 DEDGVVFRPA QMLEGLRIDD PGLRRAFRDA HPELMQPDYW EGMQHALRAG KVPKVMNYPT SRRLRR

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Lykidis A., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000283 Genomic DNA. Translation: ABE37615.1.
RefSeq	YP_567516.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q13E74.
Genome annotation databases
GeneID	4020843.
GenomeReviews	Gene locus RPD_0377 in contig CP000283_GR.
KEGG	rpd:RPD_0377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q13E74.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	RPAL316057:RPD_0377-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_RHOPS

Accession

Primary (citable) accession number: Q13E74

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 31, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)