Reviewed, UniProtKB/Swiss-Prot Q13976 (KGP1A_HUMAN)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 1, alpha isozyme
      Short name=CGK 1 alpha
      Short name=cGKI-alpha
    EC=2.7.11.12

Gene names

Name:	PRKG1
Synonyms:	PRKGR1A

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation By similarity.
Subunit structure	Antiparallel homodimer By similarity.
Miscellaneous	Exhibit a substrate specificity similar but not identical to that of CAK By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Disulfide bond Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Traceable author statement. Source: ProtInc protein amino acid phosphorylation Traceable author statement. Source: ProtInc signal transduction Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CGK1-alpha (identifier: Q13976-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CGK1-beta (identifier: P14619-1)

The sequence of this isoform can be found in the external entry P14619-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 671

670

cGMP-dependent protein kinase 1, alpha isozyme

PRO_0000086115

Regions

Domain

360 – 619

260

Protein kinase

Domain

620 – 671

52

AGC-kinase C-terminal

Nucleotide binding

103 – 220

118

cGMP 1

Nucleotide binding

221 – 341

121

cGMP 2

Nucleotide binding

366 – 374

9

ATP By similarity

Region

2 – 102

101

Dimerization By similarity

Sites

Active site

484

1

Proton acceptor By similarity

Binding site

390

1

ATP By similarity

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

59

1

Phosphothreonine; by autocatalysis By similarity

Disulfide bond

43

Interchain By similarity

Natural variations

Natural variant

267

1

N → S: dbSNP rs34997494.

VAR_051632

Secondary structure

1

.

.

.

671

Helix Strand Turn

Details...

Helix

10 – 43

34

Sequences

	Sequence		Length	Mass (Da)
	Isoform CGK1-alpha [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 51389502A5E5FBD2	FASTA	671	76,364
	10 20 30 40 50 60 MSELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR 70 80 90 100 110 120 AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY 130 140 150 160 170 180 PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT 190 200 210 220 230 240 VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPEEIL SKLADVLEET 250 260 270 280 290 300 HYENGEYIIR QGARGDTFFI ISKGTVNVTR EDSPSEDPVF LRTLGKGDWF GEKALQGEDV 310 320 330 340 350 360 RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF 370 380 390 400 410 420 NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF 430 440 450 460 470 480 IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI 490 500 510 520 530 540 IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD 550 560 570 580 590 600 YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS 610 620 630 640 650 660 ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD SFPEDNDEPP 670 PDDNSGWDID F
	Isoform CGK1-beta. See P14619.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and gene expression of human type Ialpha cGMP-dependent protein kinase." Tamura N., Itoh H., Ogawa Y., Nakagawa O., Harada M., Chun T., Suga S., Yoshimasa T., Nakao K. Hypertension 27:552-557(1996) [PubMed: 8613202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Characterization of the human gene encoding the type I alpha and type I beta cGMP-dependent protein kinase (PRKG1)." Orstavik S., Natarajan V., Tasken K., Jahnsen T., Sandberg M. Genomics 42:311-318(1997) [PubMed: 9192852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z92867 , Z92869, Z92870, Z92871, Z92872, Z92873, Z92874, Z92875, Z92876, Z92877, Z92878, Z92879, Z92880, Z92881, Z92882, Z92883, Z92884, Z92885 Genomic DNA. Translation: CAB07436.1.
D45864 mRNA. Translation: BAA08297.1.

IPI

IPI00427586.

RefSeq

NP_001091982.1.

UniGene

Hs.654556

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZXA	NMR	-	A/B	2-58	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q13976.

PTM databases

PhosphoSite

Q13976.

Proteomic databases

PRIDE

Q13976.

Genome annotation databases

Ensembl

ENST00000332193; ENSP00000327642; ENSG00000185532; Homo sapiens. [Genome view]
ENST00000373975; ENSP00000363086; ENSG00000185532; Homo sapiens. [Genome view]
ENST00000373976; ENSP00000363087; ENSG00000185532; Homo sapiens. [Genome view]
ENST00000373980; ENSP00000363092; ENSG00000185532; Homo sapiens. [Genome view]
ENST00000373985; ENSP00000363097; ENSG00000185532; Homo sapiens. [Genome view]
ENST00000401604; ENSP00000384200; ENSG00000185532; Homo sapiens. [Genome view]
ENST00000444650; ENSP00000416244; ENSG00000185532; Homo sapiens. [Genome view]

GeneID

5592.

KEGG

hsa:5592.

UCSC

uc001jjm.2. human.

Organism-specific databases

CTD

5592.

GeneCards

GC10P052421.

H-InvDB

HIX0008833.

HGNC

HGNC:9414. PRKG1.

HPA

CAB009629.
HPA007699.

MIM

176894. gene.

PharmGKB

PA33777.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q13976.

OMA

MEDGRVE.

Enzyme and pathway databases

BRENDA

2.7.11.12. 247.

Gene expression databases

ArrayExpress

Q13976.

Bgee

Q13976.

CleanEx

HS_PRKG1.

Genevestigator

Q13976.

GermOnline

ENSG00000185532. Homo sapiens.

Family and domain databases

InterPro

IPR000961. AGC-kinase_C.
IPR016232. cGMP-dependent_protein_kinase.
IPR002374. cGMP_kinase_core.
IPR000595. cNMP_bd.
IPR018488. cNMP_bd_CS.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]

Gene3D

G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.

Pfam

PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]

PIRSF

PIRSF000559. cGMP-dep_kinase. 1 hit.

PRINTS

PR00104. CGMPKINASE.

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]

PROSITE

PS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

21698.

SOURCE

Search...

Entry information

Entry name

KGP1A_HUMAN

Accession

Primary (citable) accession number: Q13976

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.