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Protein

Ras-specific guanine nucleotide-releasing factor 1

Gene

RASGRF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000058335-MONOMER.
ReactomeiR-HSA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-HSA-5673001. RAF/MAP kinase cascade.
SIGNORiQ13972.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor 1
Short name:
Ras-GRF1
Alternative name(s):
Guanine nucleotide-releasing protein
Short name:
GNRP
Ras-specific nucleotide exchange factor CDC25
Gene namesi
Name:RASGRF1
Synonyms:CDC25, GNRP, GRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:9875. RASGRF1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HGNC
  • growth cone Source: HGNC
  • neuron projection Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi5923.
OpenTargetsiENSG00000058335.
PharmGKBiPA34238.

Chemistry databases

ChEMBLiCHEMBL5468.

Polymorphism and mutation databases

DMDMi13124259.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688801 – 1273Ras-specific guanine nucleotide-releasing factor 1Add BLAST1273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei577Phosphoserine; by PLK2By similarity1
Modified residuei626Phosphoserine; by PLK2By similarity1
Modified residuei758PhosphoserineBy similarity1
Modified residuei779Phosphoserine; by PLK2By similarity1
Modified residuei800Phosphoserine; by PLK2By similarity1

Post-translational modificationi

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.By similarity
Ubiquitinated and degraded following phosphorylation by PLK2.By similarity
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ13972.
PeptideAtlasiQ13972.
PRIDEiQ13972.

PTM databases

iPTMnetiQ13972.
PhosphoSitePlusiQ13972.

Expressioni

Gene expression databases

BgeeiENSG00000058335.
CleanExiHS_RASGRF1.
GenevisibleiQ13972. HS.

Organism-specific databases

HPAiCAB010413.
HPA041965.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).By similarity

Protein-protein interaction databases

BioGridi111858. 11 interactors.
IntActiQ13972. 7 interactors.
MINTiMINT-2634734.
STRINGi9606.ENSP00000405963.

Structurei

3D structure databases

ProteinModelPortaliQ13972.
SMRiQ13972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 129PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini204 – 229IQPROSITE-ProRule annotationAdd BLAST26
Domaini240 – 426DHPROSITE-ProRule annotationAdd BLAST187
Domaini467 – 584PH 2PROSITE-ProRule annotationAdd BLAST118
Domaini644 – 761N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST118
Domaini1038 – 1270Ras-GEFPROSITE-ProRule annotationAdd BLAST233

Domaini

The DH (DBL-homology) domain mediates interaction with RASGRF2.

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ6Q. Eukaryota.
ENOG410XPWA. LUCA.
GeneTreeiENSGT00860000133664.
HOGENOMiHOG000046000.
HOVERGENiHBG005208.
InParanoidiQ13972.
KOiK04349.
OMAiDNDQNQS.
OrthoDBiEOG091G09V6.
PhylomeDBiQ13972.
TreeFamiTF317296.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
cd06224. REM. 1 hit.
Gene3Di1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030745. RasGRF1.
[Graphical view]
PANTHERiPTHR23113:SF193. PTHR23113:SF193. 4 hits.
PfamiPF00169. PH. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13972-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS
110 120 130 140 150
HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV
160 170 180 190 200
ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS TQTVAPNDED
210 220 230 240 250
SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK RNQVVFSMLE
260 270 280 290 300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
310 320 330 340 350
QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC
360 370 380 390 400
KQNRDFDKLL KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP
410 420 430 440 450
HEHVERNSLD YAKSKLEELS RIMHDEVSET ENIRKNLAIE RMIIEGCEIL
460 470 480 490 500
LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG SLSLKKEGER QCFLFSKHLI
510 520 530 540 550
ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG QDIDHLDFKI
560 570 580 590 600
GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS
610 620 630 640 650
KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY
660 670 680 690 700
ASVERLLERL TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS
710 720 730 740 750
AIPARWLRSL ELLFASGQNN KLLYGEPPKS PRATRKFSSP PPLSITKTSS
760 770 780 790 800
PSRRRKLSLN IPIITGGKAL DLAALSCNSN GYTSMYSAMS PFSKATLDTS
810 820 830 840 850
KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE ESDIDQNQSD
860 870 880 890 900
DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL
910 920 930 940 950
SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA
960 970 980 990 1000
TNRVLNVLRH WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA
1010 1020 1030 1040 1050
ANIIRTLTQE DPGDNQITLE EITQMAEGVK AEPFENHSAL EIAEQLTLLD
1060 1070 1080 1090 1100
HLVFKKIPYE EFFGQGWMKL EKNERTPYIM KTTKHFNDIS NLIASEIIRN
1110 1120 1130 1140 1150
EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA IFRLKKTWLK
1160 1170 1180 1190 1200
VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI
1210 1220 1230 1240 1250
EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD
1260 1270
QSFVMDEESL YESSLRIEPK LPT
Length:1,273
Mass (Da):145,234
Last modified:April 16, 2014 - v2
Checksum:iB629AEAFDC7E500A
GO
Isoform 2 (identifier: Q13972-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-784: Missing.

Show »
Length:489
Mass (Da):55,489
Checksum:iDDF1EDAD9793F3EF
GO
Isoform 3 (identifier: Q13972-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     610-622: Missing.
     705-707: Missing.

Show »
Length:1,257
Mass (Da):143,218
Checksum:i097E066E032DFFDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1053V → A in M91815 (PubMed:1379731).Curated1
Sequence conflicti1111E → G in M91815 (PubMed:1379731).Curated1
Sequence conflicti1134S → C in M91815 (PubMed:1379731).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0120321 – 784Missing in isoform 2. 1 PublicationAdd BLAST784
Alternative sequenceiVSP_054072610 – 622Missing in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_054073705 – 707Missing in isoform 3. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26584 mRNA. Translation: AAA58417.1.
S62035 mRNA. Translation: AAB26881.1.
AC011944 Genomic DNA. No translation available.
AC104231 Genomic DNA. No translation available.
CH471136 Genomic DNA. Translation: EAW99141.1.
AK226101 mRNA. No translation available.
M91815 mRNA. No translation available.
CCDSiCCDS10309.1. [Q13972-1]
CCDS42065.1. [Q13972-2]
CCDS45320.1. [Q13972-3]
PIRiA38985.
RefSeqiNP_002882.3. NM_002891.4. [Q13972-1]
NP_722522.1. NM_153815.2. [Q13972-2]
UniGeneiHs.459035.

Genome annotation databases

EnsembliENST00000394745; ENSP00000378228; ENSG00000058335. [Q13972-2]
ENST00000419573; ENSP00000405963; ENSG00000058335. [Q13972-1]
ENST00000558480; ENSP00000452781; ENSG00000058335. [Q13972-3]
GeneIDi5923.
KEGGihsa:5923.
UCSCiuc002beo.4. human. [Q13972-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26584 mRNA. Translation: AAA58417.1.
S62035 mRNA. Translation: AAB26881.1.
AC011944 Genomic DNA. No translation available.
AC104231 Genomic DNA. No translation available.
CH471136 Genomic DNA. Translation: EAW99141.1.
AK226101 mRNA. No translation available.
M91815 mRNA. No translation available.
CCDSiCCDS10309.1. [Q13972-1]
CCDS42065.1. [Q13972-2]
CCDS45320.1. [Q13972-3]
PIRiA38985.
RefSeqiNP_002882.3. NM_002891.4. [Q13972-1]
NP_722522.1. NM_153815.2. [Q13972-2]
UniGeneiHs.459035.

3D structure databases

ProteinModelPortaliQ13972.
SMRiQ13972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111858. 11 interactors.
IntActiQ13972. 7 interactors.
MINTiMINT-2634734.
STRINGi9606.ENSP00000405963.

Chemistry databases

ChEMBLiCHEMBL5468.

PTM databases

iPTMnetiQ13972.
PhosphoSitePlusiQ13972.

Polymorphism and mutation databases

DMDMi13124259.

Proteomic databases

PaxDbiQ13972.
PeptideAtlasiQ13972.
PRIDEiQ13972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394745; ENSP00000378228; ENSG00000058335. [Q13972-2]
ENST00000419573; ENSP00000405963; ENSG00000058335. [Q13972-1]
ENST00000558480; ENSP00000452781; ENSG00000058335. [Q13972-3]
GeneIDi5923.
KEGGihsa:5923.
UCSCiuc002beo.4. human. [Q13972-1]

Organism-specific databases

CTDi5923.
DisGeNETi5923.
GeneCardsiRASGRF1.
H-InvDBHIX0023098.
HGNCiHGNC:9875. RASGRF1.
HPAiCAB010413.
HPA041965.
MIMi606600. gene.
neXtProtiNX_Q13972.
OpenTargetsiENSG00000058335.
PharmGKBiPA34238.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ6Q. Eukaryota.
ENOG410XPWA. LUCA.
GeneTreeiENSGT00860000133664.
HOGENOMiHOG000046000.
HOVERGENiHBG005208.
InParanoidiQ13972.
KOiK04349.
OMAiDNDQNQS.
OrthoDBiEOG091G09V6.
PhylomeDBiQ13972.
TreeFamiTF317296.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000058335-MONOMER.
ReactomeiR-HSA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-HSA-5673001. RAF/MAP kinase cascade.
SIGNORiQ13972.

Miscellaneous databases

ChiTaRSiRASGRF1. human.
GenomeRNAii5923.
PROiQ13972.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000058335.
CleanExiHS_RASGRF1.
GenevisibleiQ13972. HS.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
cd06224. REM. 1 hit.
Gene3Di1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030745. RasGRF1.
[Graphical view]
PANTHERiPTHR23113:SF193. PTHR23113:SF193. 4 hits.
PfamiPF00169. PH. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGRF1_HUMAN
AccessioniPrimary (citable) accession number: Q13972
Secondary accession number(s): F8VPA5
, H0YKF2, J3KQP9, Q16027
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 16, 2014
Last modified: November 30, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.