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Protein

Ras-specific guanine nucleotide-releasing factor 1

Gene

RASGRF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

GO - Molecular functioni

  • glutamate receptor binding Source: Ensembl
  • guanyl-nucleotide exchange factor activity Source: ProtInc
  • Ras guanyl-nucleotide exchange factor activity Source: BHF-UCL
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-HSA-5673001 RAF/MAP kinase cascade
SIGNORiQ13972

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor 1
Short name:
Ras-GRF1
Alternative name(s):
Guanine nucleotide-releasing protein
Short name:
GNRP
Ras-specific nucleotide exchange factor CDC25
Gene namesi
Name:RASGRF1
Synonyms:CDC25, GNRP, GRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000058335.15
HGNCiHGNC:9875 RASGRF1
MIMi606600 gene
neXtProtiNX_Q13972

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi5923
OpenTargetsiENSG00000058335
PharmGKBiPA34238

Chemistry databases

ChEMBLiCHEMBL5468

Polymorphism and mutation databases

DMDMi13124259

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688801 – 1273Ras-specific guanine nucleotide-releasing factor 1Add BLAST1273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei577Phosphoserine; by PLK2By similarity1
Modified residuei626Phosphoserine; by PLK2By similarity1
Modified residuei758PhosphoserineBy similarity1
Modified residuei779Phosphoserine; by PLK2By similarity1
Modified residuei800Phosphoserine; by PLK2By similarity1

Post-translational modificationi

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.By similarity
Ubiquitinated and degraded following phosphorylation by PLK2.By similarity
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ13972
PeptideAtlasiQ13972
PRIDEiQ13972
ProteomicsDBi59779
59780 [Q13972-2]

PTM databases

iPTMnetiQ13972
PhosphoSitePlusiQ13972

Expressioni

Gene expression databases

BgeeiENSG00000058335
CleanExiHS_RASGRF1
GenevisibleiQ13972 HS

Organism-specific databases

HPAiCAB010413
HPA041965

Interactioni

Subunit structurei

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).By similarity

GO - Molecular functioni

  • glutamate receptor binding Source: Ensembl
  • guanyl-nucleotide exchange factor activity Source: ProtInc
  • Ras guanyl-nucleotide exchange factor activity Source: BHF-UCL
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

Protein-protein interaction databases

BioGridi111858, 11 interactors
IntActiQ13972, 7 interactors
MINTiQ13972
STRINGi9606.ENSP00000405963

Structurei

3D structure databases

ProteinModelPortaliQ13972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 129PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini204 – 229IQPROSITE-ProRule annotationAdd BLAST26
Domaini240 – 426DHPROSITE-ProRule annotationAdd BLAST187
Domaini467 – 584PH 2PROSITE-ProRule annotationAdd BLAST118
Domaini644 – 761N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST118
Domaini1038 – 1270Ras-GEFPROSITE-ProRule annotationAdd BLAST233

Domaini

The DH (DBL-homology) domain mediates interaction with RASGRF2.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ6Q Eukaryota
ENOG410XPWA LUCA
GeneTreeiENSGT00910000143985
HOGENOMiHOG000046000
HOVERGENiHBG005208
InParanoidiQ13972
KOiK04349
OMAiDPGDNQI
OrthoDBiEOG091G09V6
PhylomeDBiQ13972
TreeFamiTF317296

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
1.20.900.10, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR000048 IQ_motif_EF-hand-BS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR030745 RasGRF1
PANTHERiPTHR23113:SF193 PTHR23113:SF193, 1 hit
PfamiView protein in Pfam
PF00169 PH, 2 hits
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00233 PH, 2 hits
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 2 hits
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48366 SSF48366, 2 hits
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50096 IQ, 1 hit
PS50003 PH_DOMAIN, 2 hits
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13972-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS
110 120 130 140 150
HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV
160 170 180 190 200
ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS TQTVAPNDED
210 220 230 240 250
SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK RNQVVFSMLE
260 270 280 290 300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
310 320 330 340 350
QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC
360 370 380 390 400
KQNRDFDKLL KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP
410 420 430 440 450
HEHVERNSLD YAKSKLEELS RIMHDEVSET ENIRKNLAIE RMIIEGCEIL
460 470 480 490 500
LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG SLSLKKEGER QCFLFSKHLI
510 520 530 540 550
ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG QDIDHLDFKI
560 570 580 590 600
GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS
610 620 630 640 650
KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY
660 670 680 690 700
ASVERLLERL TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS
710 720 730 740 750
AIPARWLRSL ELLFASGQNN KLLYGEPPKS PRATRKFSSP PPLSITKTSS
760 770 780 790 800
PSRRRKLSLN IPIITGGKAL DLAALSCNSN GYTSMYSAMS PFSKATLDTS
810 820 830 840 850
KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE ESDIDQNQSD
860 870 880 890 900
DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL
910 920 930 940 950
SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA
960 970 980 990 1000
TNRVLNVLRH WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA
1010 1020 1030 1040 1050
ANIIRTLTQE DPGDNQITLE EITQMAEGVK AEPFENHSAL EIAEQLTLLD
1060 1070 1080 1090 1100
HLVFKKIPYE EFFGQGWMKL EKNERTPYIM KTTKHFNDIS NLIASEIIRN
1110 1120 1130 1140 1150
EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA IFRLKKTWLK
1160 1170 1180 1190 1200
VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI
1210 1220 1230 1240 1250
EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD
1260 1270
QSFVMDEESL YESSLRIEPK LPT
Length:1,273
Mass (Da):145,234
Last modified:April 16, 2014 - v2
Checksum:iB629AEAFDC7E500A
GO
Isoform 2 (identifier: Q13972-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-784: Missing.

Show »
Length:489
Mass (Da):55,489
Checksum:iDDF1EDAD9793F3EF
GO
Isoform 3 (identifier: Q13972-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     610-622: Missing.
     705-707: Missing.

Show »
Length:1,257
Mass (Da):143,218
Checksum:i097E066E032DFFDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1053V → A in M91815 (PubMed:1379731).Curated1
Sequence conflicti1111E → G in M91815 (PubMed:1379731).Curated1
Sequence conflicti1134S → C in M91815 (PubMed:1379731).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0120321 – 784Missing in isoform 2. 1 PublicationAdd BLAST784
Alternative sequenceiVSP_054072610 – 622Missing in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_054073705 – 707Missing in isoform 3. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26584 mRNA Translation: AAA58417.1
S62035 mRNA Translation: AAB26881.1
AC011944 Genomic DNA No translation available.
AC104231 Genomic DNA No translation available.
CH471136 Genomic DNA Translation: EAW99141.1
AK226101 mRNA No translation available.
M91815 mRNA No translation available.
CCDSiCCDS10309.1 [Q13972-1]
CCDS42065.1 [Q13972-2]
CCDS45320.1 [Q13972-3]
PIRiA38985
RefSeqiNP_002882.3, NM_002891.4 [Q13972-1]
NP_722522.1, NM_153815.2 [Q13972-2]
UniGeneiHs.459035

Genome annotation databases

EnsembliENST00000394745; ENSP00000378228; ENSG00000058335 [Q13972-2]
ENST00000419573; ENSP00000405963; ENSG00000058335 [Q13972-1]
ENST00000558480; ENSP00000452781; ENSG00000058335 [Q13972-3]
GeneIDi5923
KEGGihsa:5923
UCSCiuc002beo.4 human [Q13972-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRGRF1_HUMAN
AccessioniPrimary (citable) accession number: Q13972
Secondary accession number(s): F8VPA5
, H0YKF2, J3KQP9, Q16027
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 16, 2014
Last modified: June 20, 2018
This is version 179 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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