Q13952 (NFYC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear transcription factor Y subunit gamma

Alternative name(s):
CAAT box DNA-binding protein subunit C
Nuclear transcription factor Y subunit C
Short name=NF-YC
Transactivator HSM-1/2

Gene names

Name:

NFYC

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
Subunit structure	Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the NFYC/HAP5 subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Traceable author statement. Source: ProtInc regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	CCAAT-binding factor complex Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc transcription coactivator activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NFYA	P23511	3	EBI-389755,EBI-389739
NFYB	P25208	3	EBI-389755,EBI-389728

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: Q13952-1)

Also known as: DS2.8;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q13952-2)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     277-295: Missing.
     316-419: Missing.

Isoform 2 (identifier: Q13952-3)

The sequence of this isoform differs from the canonical sequence as follows:
     277-295: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 458

458

Nuclear transcription factor Y subunit gamma

PRO_0000218246

Natural variations

Alternative sequence

277 – 295

19

Missing in isoform 1 and isoform 2.

VSP_000851

Alternative sequence

316 – 419

104

Missing in isoform 1.

VSP_000852

Natural variant

165

1

Q → H in a breast cancer sample; somatic mutation. Ref.13

VAR_035702

Natural variant

297

1

T → I.
Corresponds to variant rs2230746 [ dbSNP | Ensembl ].

VAR_059460

Experimental info

Sequence conflict

52

1

M → I in CAA99055. Ref.3

Sequence conflict

90

1

D → N in BAA14051. Ref.4

Sequence conflict

155

1

V → L in AAC50816. Ref.1

Sequence conflict

158

1

Q → H in BAA14051. Ref.4

Sequence conflict

171

1

T → N in BAA14051. Ref.4

Sequence conflict

175

1

P → A in AAC50816. Ref.1

Sequence conflict

179

1

I → F in BAA14051. Ref.4

Sequence conflict

198

1

G → S in BAA12818. Ref.4

Sequence conflict

198

1

G → S in BAA14051. Ref.4

Sequence conflict

202

1

Q → K in BAA12818. Ref.4

Sequence conflict

216 – 217

2

QS → HN in BAA14051. Ref.4

Sequence conflict

248

1

L → V in AAC50816. Ref.1

Sequence conflict

271

1

A → V in AAC50816. Ref.1

Sequence conflict

451

1

Q → K in BAA14051. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

458

Helix Strand Turn

Details...

Helix

45 – 52

8

Helix

64 – 90

27

Beta strand

94 – 96

3

Helix

98 – 105

8

Helix

109 – 114

6

Turn

115 – 117

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 (DS2.8) [UniParc]. Last modified January 23, 2002. Version 3. Checksum: B17B2C7F622653B9	FASTA	458	50,302
	10 20 30 40 50 60 MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK IMKLDEDVKM 70 80 90 100 110 120 ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND IAMAITKFDQ FDFLIDIVPR 130 140 150 160 170 180 DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ QPTAVQVQGQ QQGQQTTSST TTIQPGQIII 190 200 210 220 230 240 AQPQQGQTTP VTMQVGEGQQ VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP 250 260 270 280 290 300 VQLNAGQLQY IRLAQPVSGT QVVQGQIQTL ATNAQQGQRN ASQGKPRRCL KETLQITQTE 310 320 330 340 350 360 VQQGQQQFSQ FTDGQRNSVQ QARVSELTGE AEPREVKATG NSTPCTSSLP TTHPPSHRAG 370 380 390 400 410 420 ASCVCCSQPQ QSSTSPPPSD ALQWVVVEVS GTPNQLETHR ELHAPLPGMT SLSPLHPSQQ 430 440 450 LYQIQQVTMP AGQDLAQPMF IQSANQPSDG QAPQVTGD
	Isoform 1 (Gamma) [UniParc]. Checksum: D83635C1773C3895	FASTA	335	37,194
	10 20 30 40 50 60 MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK IMKLDEDVKM 70 80 90 100 110 120 ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND IAMAITKFDQ FDFLIDIVPR 130 140 150 160 170 180 DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ QPTAVQVQGQ QQGQQTTSST TTIQPGQIII 190 200 210 220 230 240 AQPQQGQTTP VTMQVGEGQQ VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP 250 260 270 280 290 300 VQLNAGQLQY IRLAQPVSGT QVVQGQIQTL ATNAQQITQT EVQQGQQQFS QFTDGQQLYQ 310 320 330 IQQVTMPAGQ DLAQPMFIQS ANQPSDGQAP QVTGD
	Isoform 2 [UniParc]. Checksum: E3FED3512983A402	FASTA	439	48,150
	10 20 30 40 50 60 MSTEGGFGGT SSSDAQQSLQ SFWPRVMEEI RNLTVKDFRV QELPLARIKK IMKLDEDVKM 70 80 90 100 110 120 ISAEAPVLFA KAAQIFITEL TLRAWIHTED NKRRTLQRND IAMAITKFDQ FDFLIDIVPR 130 140 150 160 170 180 DELKPPKRQE EVRQSVTPAE PVQYYFTLAQ QPTAVQVQGQ QQGQQTTSST TTIQPGQIII 190 200 210 220 230 240 AQPQQGQTTP VTMQVGEGQQ VQIVQAQPQG QAQQAQSGTG QTMQVMQQII TNTGEIQQIP 250 260 270 280 290 300 VQLNAGQLQY IRLAQPVSGT QVVQGQIQTL ATNAQQITQT EVQQGQQQFS QFTDGQRNSV 310 320 330 340 350 360 QQARVSELTG EAEPREVKAT GNSTPCTSSL PTTHPPSHRA GASCVCCSQP QQSSTSPPPS 370 380 390 400 410 420 DALQWVVVEV SGTPNQLETH RELHAPLPGM TSLSPLHPSQ QLYQIQQVTM PAGQDLAQPM 430 FIQSANQPSD GQAPQVTGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subunit association and DNA binding activity of the heterotrimeric transcription factor NF-Y is regulated by cellular redox." Nakshatri H., Bhat-Nakshatri P., Currie R.A. J. Biol. Chem. 271:28784-28791(1996) [PubMed: 8910521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning and expression of human NF-YC." Bellorini M., Zemzoumi K., Farina A., Berthelsen J., Piaggio G., Mantovani R. Gene 193:119-125(1997) [PubMed: 9249075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Isolation and sequence analysis of the cDNA encoding subunit C of human CCAAT-binding transcription factor." Dmitrenko V.V., Garifulin O.M., Kavsan V.M. Gene 197:161-163(1997) [PubMed: 9332362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Cell cycle-dependent switch of up- and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y." Taira T., Sawai M., Ikeda M., Tamai K., Iguchi-Ariga S.M.M., Ariga H. J. Biol. Chem. 274:24270-24279(1999) [PubMed: 10446203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"Cloning of a new variant of NFY-C." Bringuier P.P., Schalken J.A., Yamasaki H., Giroldi L.A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y." Romier C., Cocchiarella F., Mantovani R., Moras D. J. Biol. Chem. 278:1336-1345(2003) [PubMed: 12401788] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.67 ANGSTROMS) OF 27-120 IN COMPLEX WITH NF-YB.
[13]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-165.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U62296 mRNA. Translation: AAC50816.1.
U78774 mRNA. Translation: AAC51669.1.
Z74792 mRNA. Translation: CAA99055.1.
D85425 mRNA. Translation: BAA12818.1.
D89986 mRNA. Translation: BAA14051.1.
AF191744 mRNA. Translation: AAG28389.1.
AK000346 mRNA. Translation: BAA91100.1.
BT020081 mRNA. Translation: AAV38884.1.
AL354914, AC119677 Genomic DNA. Translation: CAI16530.1.
AL354914, AC119677 Genomic DNA. Translation: CAI16531.1.
AL354914, AC119677 Genomic DNA. Translation: CAI16533.1.
CH471059 Genomic DNA. Translation: EAX07198.1.
CH471059 Genomic DNA. Translation: EAX07201.1.
CH471059 Genomic DNA. Translation: EAX07202.1.
BC005003 mRNA. Translation: AAH05003.1.

IPI

IPI00071697.
IPI00216407.
IPI00911001.

RefSeq

NP_001136059.1. NM_001142587.1.
NP_001136060.1. NM_001142588.1.
NP_001136061.1. NM_001142589.1.
NP_001136062.1. NM_001142590.1.
NP_055038.2. NM_014223.4.

UniGene

Hs.713051.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N1J	X-ray	1.67	B	27-120	[»]

ProteinModelPortal

Q13952.

SMR

Q13952. Positions 43-120.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13952. 19 interactions.

MINT

MINT-249381.

STRING

Q13952.

Polymorphism databases

DMDM

20137773.

Proteomic databases

PRIDE

Q13952.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000308733; ENSP00000312617; ENSG00000066136.
ENST00000372657; ENSP00000361741; ENSG00000066136.

GeneID

4802.

KEGG

hsa:4802.

UCSC

uc001cfy.2. human.
uc001cgb.2. human.
uc001cge.2. human.

Organism-specific databases

CTD

4802.

GeneCards

GC01P041192.

HGNC

HGNC:7806. NFYC.

MIM

605344. gene.

neXtProt

NX_Q13952.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG04809.

HOVERGEN

HBG002885.

InParanoid

Q13952.

OMA

ATNAQQI.

PhylomeDB

Q13952.

Gene expression databases

ArrayExpress

Q13952.

Bgee

Q13952.

Genevestigator

Q13952.

GermOnline

ENSG00000066136. Homo sapiens.

Family and domain databases

InterPro

IPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]

Gene3D

G3DSA:1.10.20.10. Histone-fold. 1 hit.

KO

K08066.

Pfam

PF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]

SUPFAM

SSF47113. Histone-fold. 1 hit.

ProtoNet

Search...

Other

NextBio

18510.

SOURCE

Search...

Entry information

Entry name

NFYC_HUMAN

Accession

Primary (citable) accession number: Q13952
Secondary accession number(s): D3DPV9 , Q5T6K8, Q5T6L1, Q5TZR6, Q92869, Q9HBX1, Q9NXB5, Q9UM67, Q9UML0, Q9UMT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	January 25, 2012
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.