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Protein

General transcription factor IIH subunit 3

Gene

GTF2H3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Anchors XPB.Curated1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri268 – 285C4-typeAdd BLAST18

GO - Molecular functioni

  • damaged DNA binding Source: ProtInc
  • metal ion binding Source: UniProtKB-KW
  • protein N-terminus binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc
  • translation factor activity, RNA binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE during HIV infection.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-5696395. Formation of Incision Complex in GG-NER.
R-HSA-5696400. Dual Incision in GG-NER.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-72086. mRNA Capping.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-73863. RNA Polymerase I Transcription Termination.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 3
Alternative name(s):
Basic transcription factor 2 34 kDa subunit
Short name:
BTF2 p341 Publication
General transcription factor IIH polypeptide 3
TFIIH basal transcription factor complex p34 subunit1 Publication
Gene namesi
Name:GTF2H3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:4657. GTF2H3.

Subcellular locationi

GO - Cellular componenti

  • core TFIIH complex portion of holo TFIIH complex Source: UniProtKB
  • holo TFIIH complex Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • transcriptional preinitiation complex Source: UniProtKB
  • transcription factor TFIID complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2967.
OpenTargetsiENSG00000111358.
PharmGKBiPA29043.

Polymorphism and mutation databases

DMDMi50403772.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192511 – 308General transcription factor IIH subunit 3Add BLAST308

Proteomic databases

EPDiQ13889.
MaxQBiQ13889.
PaxDbiQ13889.
PeptideAtlasiQ13889.
PRIDEiQ13889.

PTM databases

iPTMnetiQ13889.
PhosphoSitePlusiQ13889.

Expressioni

Gene expression databases

BgeeiENSG00000111358.
CleanExiHS_GTF2H3.
ExpressionAtlasiQ13889. baseline and differential.
GenevisibleiQ13889. HS.

Organism-specific databases

HPAiHPA004844.
HPA053562.

Interactioni

Subunit structurei

Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27193682). One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor (PubMed:9852112). Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GTF2H2C_2Q6P1K83EBI-6380459,EBI-8469755

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109222. 18 interactors.
DIPiDIP-787N.
IntActiQ13889. 2 interactors.
MINTiMINT-3028878.
STRINGi9606.ENSP00000445162.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IVWelectron microscopy10.0031-308[»]
5IY6electron microscopy7.2031-308[»]
5IY7electron microscopy8.6031-308[»]
5IY8electron microscopy7.9031-308[»]
5IY9electron microscopy6.3031-308[»]
ProteinModelPortaliQ13889.
SMRiQ13889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri268 – 285C4-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2487. Eukaryota.
COG5242. LUCA.
GeneTreeiENSGT00390000013143.
HOGENOMiHOG000175900.
HOVERGENiHBG054271.
InParanoidiQ13889.
KOiK03143.
OMAiFGNAHLM.
OrthoDBiEOG091G0GIC.
PhylomeDBiQ13889.
TreeFamiTF314336.

Family and domain databases

InterProiView protein in InterPro
IPR004600. TFIIH_Tfb4/p34.
PANTHERiPTHR12831. PTHR12831. 1 hit.
PfamiView protein in Pfam
PF03850. Tfb4. 1 hit.
TIGRFAMsiTIGR00627. tfb4. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13889-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF
60 70 80 90 100
MNRSNKLAVI ASHIQESRFL YPGKNGRLGD FFGDPGNPPE FNPSGSKDGK
110 120 130 140 150
YELLTSANEV IVEEIKDLMT KSDIKGQHTE TLLAGSLAKA LCYIHRMNKE
160 170 180 190 200
VKDNQEMKSR ILVIKAAEDS ALQYMNFMNV IFAAQKQNIL IDACVLDSDS
210 220 230 240 250
GLLQQACDIT GGLYLKVPQM PSLLQYLLWV FLPDQDQRSQ LILPPPVHVD
260 270 280 290 300
YRAACFCHRN LIEIGYVCSV CLSIFCNFSP ICTTCETAFK ISLPPVLKAK

KKKLKVSA
Length:308
Mass (Da):34,378
Last modified:July 19, 2004 - v2
Checksum:iE739E2176CD6BAA5
GO
Isoform 2 (identifier: Q13889-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Note: No experimental confirmation available.
Show »
Length:267
Mass (Da):29,777
Checksum:i9415C0930FAADD34
GO

Sequence cautioni

The sequence CAA82909 differs from that shown. Reason: Frameshift at position 302.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0551531 – 41Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK298128 mRNA. Translation: BAG60408.1.
AK313200 mRNA. Translation: BAG36016.1.
AF548661 Genomic DNA. Translation: AAN40702.1.
AC117503 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW98426.1.
BC039726 mRNA. Translation: AAH39726.1.
BC047868 mRNA. Translation: AAH47868.2.
BC065250 mRNA. Translation: AAH65250.1.
Z30093 mRNA. Translation: CAA82909.1. Frameshift.
CCDSiCCDS61275.1. [Q13889-2]
CCDS9252.1. [Q13889-1]
PIRiS44455.
RefSeqiNP_001258795.1. NM_001271866.1.
NP_001258796.1. NM_001271867.1. [Q13889-2]
NP_001258797.1. NM_001271868.1.
NP_001507.2. NM_001516.4. [Q13889-1]
UniGeneiHs.355348.

Genome annotation databases

EnsembliENST00000228955; ENSP00000228955; ENSG00000111358. [Q13889-2]
ENST00000543341; ENSP00000445162; ENSG00000111358. [Q13889-1]
GeneIDi2967.
KEGGihsa:2967.
UCSCiuc001ufo.3. human. [Q13889-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTF2H3_HUMAN
AccessioniPrimary (citable) accession number: Q13889
Secondary accession number(s): B2R819
, B4DNZ6, Q7L0G0, Q96AT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 19, 2004
Last modified: June 7, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families