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Q13889 (TF2H3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
General transcription factor IIH subunit 3
Alternative name(s):
Basic transcription factor 2 34 kDa subunit
Short name=BTF2 p34
General transcription factor IIH polypeptide 3
TFIIH basal transcription factor complex p34 subunit
Gene names
Name:GTF2H3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Anchors XPB.

Subunit structure

One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7 By similarity. Ref.6

Subcellular location

Nucleus.

Sequence similarities

Belongs to the TFB4 family.

Sequence caution

The sequence CAA82909.1 differs from that shown. Reason: Frameshift at position 302.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_process7-methylguanosine mRNA capping

Traceable author statement. Source: Reactome

ATP catabolic process

Inferred from direct assay Ref.6. Source: GOC

DNA repair

Traceable author statement. Source: Reactome

gene expression

Traceable author statement. Source: Reactome

nucleotide-excision repair

Traceable author statement. Source: Reactome

nucleotide-excision repair, DNA damage removal

Traceable author statement. Source: Reactome

positive regulation of viral transcription

Traceable author statement. Source: Reactome

protein phosphorylation

Inferred from direct assay Ref.6. Source: GOC

termination of RNA polymerase I transcription

Traceable author statement. Source: Reactome

transcription elongation from RNA polymerase I promoter

Traceable author statement. Source: Reactome

transcription elongation from RNA polymerase II promoter

Traceable author statement. Source: Reactome

transcription from RNA polymerase I promoter

Traceable author statement. Source: Reactome

transcription from RNA polymerase II promoter

Inferred from direct assay Ref.6. Source: UniProtKB

transcription initiation from RNA polymerase I promoter

Traceable author statement. Source: Reactome

transcription initiation from RNA polymerase II promoter

Traceable author statement. Source: Reactome

transcription-coupled nucleotide-excision repair

Traceable author statement. Source: Reactome

translation

Traceable author statement Ref.3. Source: GOC

viral process

Traceable author statement. Source: Reactome

   Cellular_componentcore TFIIH complex

Inferred from electronic annotation. Source: InterPro

holo TFIIH complex

Inferred from direct assay Ref.6. Source: UniProtKB

nucleoplasm

Traceable author statement. Source: Reactome

   Molecular_functiondamaged DNA binding

Traceable author statement Ref.3. Source: ProtInc

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein N-terminus binding

Inferred from physical interaction PubMed 8652557. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Non-traceable author statement Ref.3. Source: ProtInc

translation factor activity, nucleic acid binding

Traceable author statement Ref.3. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308General transcription factor IIH subunit 3
PRO_0000119251

Regions

Zinc finger268 – 28518C4-type

Sequences

Sequence LengthMass (Da)Tools
Q13889 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: E739E2176CD6BAA5

FASTA30834,378
        10         20         30         40         50         60 
MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI 

        70         80         90        100        110        120 
ASHIQESRFL YPGKNGRLGD FFGDPGNPPE FNPSGSKDGK YELLTSANEV IVEEIKDLMT 

       130        140        150        160        170        180 
KSDIKGQHTE TLLAGSLAKA LCYIHRMNKE VKDNQEMKSR ILVIKAAEDS ALQYMNFMNV 

       190        200        210        220        230        240 
IFAAQKQNIL IDACVLDSDS GLLQQACDIT GGLYLKVPQM PSLLQYLLWV FLPDQDQRSQ 

       250        260        270        280        290        300 
LILPPPVHVD YRAACFCHRN LIEIGYVCSV CLSIFCNFSP ICTTCETAFK ISLPPVLKAK 


KKKLKVSA 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair."
Humbert S., van Vuuren H.A., Lutz Y., Hoeijmakers J.H.J., Egly J.-M., Moncollin V.
EMBO J. 13:2393-2398(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-305, PROTEIN SEQUENCE OF 57-68; 78-97; 107-112; 127-132 AND 239-251.
[4]NIEHS SNPs program
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Eye and Testis.
[6]"Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
Kershnar E., Wu S.-Y., Chiang C.-M.
J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TFIIH BASAL TRANSCRIPTION FACTOR.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK313200 mRNA. Translation: BAG36016.1.
CH471054 Genomic DNA. Translation: EAW98426.1.
Z30093 mRNA. Translation: CAA82909.1. Frameshift.
AF548661 Genomic DNA. Translation: AAN40702.1.
BC039726 mRNA. Translation: AAH39726.1.
BC047868 mRNA. Translation: AAH47868.2.
BC065250 mRNA. Translation: AAH65250.1.
PIRS44455.
RefSeqNP_001258795.1. NM_001271866.1.
NP_001258796.1. NM_001271867.1.
NP_001258797.1. NM_001271868.1.
NP_001507.2. NM_001516.4.
UniGeneHs.355348.

3D structure databases

ProteinModelPortalQ13889.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109222. 11 interactions.
DIPDIP-787N.
IntActQ13889. 1 interaction.
MINTMINT-3028878.
STRING9606.ENSP00000228955.

PTM databases

PhosphoSiteQ13889.

Polymorphism databases

DMDM50403772.

Proteomic databases

PaxDbQ13889.
PRIDEQ13889.

Protocols and materials databases

DNASU2967.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000543341; ENSP00000445162; ENSG00000111358.
GeneID2967.
KEGGhsa:2967.
UCSCuc001ufo.2. human.

Organism-specific databases

CTD2967.
GeneCardsGC12P124118.
H-InvDBHIX0026378.
HGNCHGNC:4657. GTF2H3.
HPAHPA004844.
HPA053562.
MIM601750. gene.
neXtProtNX_Q13889.
PharmGKBPA29043.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5242.
HOGENOMHOG000175900.
HOVERGENHBG054271.
InParanoidQ13889.
KOK03143.
OMAMVMGNSH.
PhylomeDBQ13889.
TreeFamTF314336.

Enzyme and pathway databases

ReactomeREACT_116125. Disease.
REACT_1788. Transcription.
REACT_216. DNA Repair.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ13889.
BgeeQ13889.
CleanExHS_GTF2H3.
GenevestigatorQ13889.

Family and domain databases

InterProIPR004600. TFIIH_Tfb4/p34.
[Graphical view]
PANTHERPTHR12831. PTHR12831. 1 hit.
PfamPF03850. Tfb4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00627. tfb4. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi2967.
NextBio11760.
PROQ13889.
SOURCESearch...

Entry information

Entry nameTF2H3_HUMAN
AccessionPrimary (citable) accession number: Q13889
Secondary accession number(s): B2R819, Q7L0G0, Q96AT7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM