Q13889 (TF2H3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General transcription factor IIH subunit 3

Alternative name(s):
Basic transcription factor 2 34 kDa subunit
Short name=BTF2 p34
General transcription factor IIH polypeptide 3
TFIIH basal transcription factor complex p34 subunit

Gene names

Name:

GTF2H3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Anchors XPB.
Subunit structure	One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7 By similarity. Ref.6
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TFB4 family.
Sequence caution	The sequence CAA82909.1 differs from that shown. Reason: Frameshift at position 302.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Traceable author statement. Source: Reactome nucleotide-excision repair, DNA damage removal Traceable author statement. Source: Reactome positive regulation of viral transcription Traceable author statement. Source: Reactome protein phosphorylation Inferred from direct assay Ref.6. Source: GOC termination of RNA polymerase I transcription Traceable author statement. Source: Reactome transcription elongation from RNA polymerase I promoter Traceable author statement. Source: Reactome transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase I promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription-coupled nucleotide-excision repair Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	core TFIIH complex Inferred from electronic annotation. Source: InterPro holo TFIIH complex Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	damaged DNA binding Non-traceable author statement. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Non-traceable author statement. Source: UniProtKB translation factor activity, nucleic acid binding Traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	General transcription factor IIH subunit 3		PRO_0000119251
Regions
	Zinc finger	268 – 285	18	C4-type

Sequences

	Sequence		Length	Mass (Da)
	Q13889 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: E739E2176CD6BAA5	FASTA	308	34,378
	10 20 30 40 50 60 MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI 70 80 90 100 110 120 ASHIQESRFL YPGKNGRLGD FFGDPGNPPE FNPSGSKDGK YELLTSANEV IVEEIKDLMT 130 140 150 160 170 180 KSDIKGQHTE TLLAGSLAKA LCYIHRMNKE VKDNQEMKSR ILVIKAAEDS ALQYMNFMNV 190 200 210 220 230 240 IFAAQKQNIL IDACVLDSDS GLLQQACDIT GGLYLKVPQM PSLLQYLLWV FLPDQDQRSQ 250 260 270 280 290 300 LILPPPVHVD YRAACFCHRN LIEIGYVCSV CLSIFCNFSP ICTTCETAFK ISLPPVLKAK KKKLKVSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair." Humbert S., van Vuuren H.A., Lutz Y., Hoeijmakers J.H.J., Egly J.-M., Moncollin V. EMBO J. 13:2393-2398(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-305, PROTEIN SEQUENCE OF 57-68; 78-97; 107-112; 127-132 AND 239-251.
[4]	NIEHS SNPs program Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Eye and Testis.
[6]	"Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes." Kershnar E., Wu S.-Y., Chiang C.-M. J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TFIIH BASAL TRANSCRIPTION FACTOR.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK313200 mRNA. Translation: BAG36016.1. CH471054 Genomic DNA. Translation: EAW98426.1. Z30093 mRNA. Translation: CAA82909.1. Frameshift. AF548661 Genomic DNA. Translation: AAN40702.1. BC039726 mRNA. Translation: AAH39726.1. BC047868 mRNA. Translation: AAH47868.2. BC065250 mRNA. Translation: AAH65250.1.
IPI	IPI00100791.
PIR	S44455.
RefSeq	NP_001258795.1. NM_001271866.1. NP_001258796.1. NM_001271867.1. NP_001258797.1. NM_001271868.1. NP_001507.2. NM_001516.4.
UniGene	Hs.355348.
3D structure databases
ProteinModelPortal	Q13889.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-787N.
STRING	9606.ENSP00000228955.
PTM databases
PhosphoSite	Q13889.
Polymorphism databases
DMDM	50403772.
Proteomic databases
PaxDb	Q13889.
PRIDE	Q13889.
Protocols and materials databases
DNASU	2967.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000543341; ENSP00000445162; ENSG00000111358.
GeneID	2967.
KEGG	hsa:2967.
UCSC	uc001ufo.1. human.
Organism-specific databases
CTD	2967.
GeneCards	GC12P124118.
H-InvDB	HIX0026378.
HGNC	HGNC:4657. GTF2H3.
HPA	HPA004844. HPA053562.
MIM	601750. gene.
neXtProt	NX_Q13889.
PharmGKB	PA29043.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5242.
HOGENOM	HOG000175900.
HOVERGEN	HBG054271.
InParanoid	Q13889.
KO	K03143.
OMA	NAHLMQK.
OrthoDB	EOG47PX6J.
PhylomeDB	Q13889.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_216. DNA Repair. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q13889.
Bgee	Q13889.
CleanEx	HS_GTF2H3.
Genevestigator	Q13889.
Family and domain databases
InterPro	IPR004600. TFIIH_Tfb4/p34. [Graphical view]
PANTHER	PTHR12831. PTHR12831. 1 hit.
Pfam	PF03850. Tfb4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00627. tfb4. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	2967.
NextBio	11760.
SOURCE	Search...

Entry information

Entry name

TF2H3_HUMAN

Accession

Primary (citable) accession number: Q13889
Secondary accession number(s): B2R819, Q7L0G0, Q96AT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.