Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q13887 (KLF5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Krueppel-like factor 5
Alternative name(s):
Basic transcription element-binding protein 2
Short name=BTE-binding protein 2
Colon krueppel-like factor
GC-box-binding protein 2
Intestinal-enriched krueppel-like factor
Transcription factor BTEB2
Gene names
Name:KLF5
Synonyms:BTEB2, CKLF, IKLF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Subunit structure

Interacts with WWP1. Ref.7

Subcellular location

Nucleus Ref.7.

Tissue specificity

Expressed only in testis and placenta.

Post-translational modification

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. Ref.7

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMUbl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from electronic annotation. Source: Ensembl

cellular response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

cellular response to peptide

Inferred from electronic annotation. Source: Ensembl

microvillus assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

skeletal muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

transcription from RNA polymerase II promoter

Traceable author statement Ref.6. Source: ProtInc

   Cellular_componentGolgi apparatus

Inferred from direct assay. Source: HPA

cytoplasm

Inferred from direct assay. Source: HPA

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from direct assay. Source: HPA

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 17320083. Source: IntAct

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

UBCP0CG482EBI-2696013,EBI-3390054

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13887-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13887-2)

The sequence of this isoform differs from the canonical sequence as follows:
     88-239: Missing.
Isoform 3 (identifier: Q13887-3)

The sequence of this isoform differs from the canonical sequence as follows:
     105-166: IIPEHKKYRR...CVTHIKTEPV → MLLQLLLNWQ...AQKFIPSLLI
     167-457: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457Krueppel-like factor 5
PRO_0000047169

Regions

Zinc finger373 – 39725C2H2-type 1
Zinc finger403 – 42725C2H2-type 2
Zinc finger433 – 45523C2H2-type 3
Region324 – 3285Interaction with WWP1

Natural variations

Alternative sequence88 – 239152Missing in isoform 2.
VSP_047474
Alternative sequence105 – 16662IIPEH…KTEPV → MLLQLLLNWQFTIQIYPPPC QLTHKTSNLSDTIEGVTPIW RNDASTTAITLVAQKFIPSL LI in isoform 3.
VSP_047475
Alternative sequence167 – 457291Missing in isoform 3.
VSP_047476
Natural variant3011P → S in a colorectal cancer sample; somatic mutation. Ref.9
VAR_035555

Experimental info

Mutagenesis324 – 3285Missing: Impairs ubiquitination and degradation. Ref.7

Secondary structure

................ 457
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 3BF12BE272716E57

FASTA45750,792
        10         20         30         40         50         60 
MATRVLSMSA RLGPVPQPPA PQDEPVFAQL KPVLGAANPA RDAALFPGEE LKHAHHRPQA 

        70         80         90        100        110        120 
QPAPAQAPQP AQPPATGPRL PPEDLVQTRC EMEKYLTPQL PPVPIIPEHK KYRRDSASVV 

       130        140        150        160        170        180 
DQFFTDTEGL PYSINMNVFL PDITHLRTGL YKSQRPCVTH IKTEPVAIFS HQSETTAPPP 

       190        200        210        220        230        240 
APTQALPEFT SIFSSHQTAA PEVNNIFIKQ ELPTPDLHLS VPTQQGHLYQ LLNTPDLDMP 

       250        260        270        280        290        300 
SSTNQTAAMD TLNVSMSAAM AGLNTHTSAV PQTAVKQFQG MPPCTYTMPS QFLPQQATYF 

       310        320        330        340        350        360 
PPSPPSSEPG SPDRQAEMLQ NLTPPPSYAA TIASKLAIHN PNLPTTLPVN SQNIQPVRYN 

       370        380        390        400        410        420 
RRSNPDLEKR RIHYCDYPGC TKVYTKSSHL KAHLRTHTGE KPYKCTWEGC DWRFARSDEL 

       430        440        450 
TRHYRKHTGA KPFQCGVCNR SFSRSDHLAL HMKRHQN 

« Hide

Isoform 2 [UniParc].

Checksum: 0C584B65B6ED3BD1
Show »

FASTA30533,618
Isoform 3 [UniParc].

Checksum: 4210629ABFC9175C
Show »

FASTA16618,116

References

« Hide 'large scale' references
[1]"Isolation and characterization of a gene encoding human Kruppel-like factor 5 (IKLF): binding to the CAAT/GT box of the mouse lactoferrin gene promoter."
Shi H., Zhang Z., Wang X., Liu S., Teng C.T.
Nucleic Acids Res. 27:4807-4815(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Shaking the family tree: Identification of novel and biologically active alternatively spliced isoforms across the KLF family of transcription factors."
Camacho-Vanegas O., Till J., Miranda-Lorenzo I., Ozturk B., Camacho S.C., Martignetti J.A.
FASEB J. 27:432-436(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), ALTERNATIVE SPLICING.
[3]"Human basic transcription element binding protein 2."
Ohnishi S., Yoshida T., Ramirez F., Terada M., Friedrich L.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Human Kruppel like factor5/KLF5: expression in human skin and hair follicles."
Sur I., Unden A.-B., Toftgard R.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Placenta.
[6]"cDNA cloning and transcriptional properties of a novel GC box-binding protein, BTEB2."
Sogawa K., Imataka H., Yamasaki Y., Kusume H., Abe H., Fujii-Kuriyama Y.
Nucleic Acids Res. 21:1527-1532(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 239-457 (ISOFORM 1).
Tissue: Placenta.
[7]"Ubiquitin-proteasome degradation of KLF5 transcription factor in cancer and untransformed epithelial cells."
Chen C., Sun X., Ran Q., Wilkinson K.D., Murphy T.J., Simons J.W., Dong J.T.
Oncogene 24:3319-3327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH WWP1, UBIQUITINATION, MUTAGENESIS OF 324-PRO--TYR-328.
[8]"Solution structure of three tandem repeats of ZF-C2H2 domains from human kruppel-like factor 5."
RIKEN structural genomics initiative (RSGI)
Submitted (FEB-2008) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 363-457.
[9]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-301.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF132818 mRNA. Translation: AAF18307.1.
HF546203 mRNA. Translation: CCO02789.1.
HF546204 mRNA. Translation: CCO02790.1.
AB030824 mRNA. Translation: BAA96461.1.
AF287272 mRNA. Translation: AAF88068.1.
BC007695 mRNA. Translation: AAH07695.1.
BC042131 mRNA. Translation: AAH42131.1.
D14520 mRNA. Translation: BAA03393.1.
CCDSCCDS9448.1. [Q13887-1]
PIRS35643.
RefSeqNP_001273747.1. NM_001286818.1.
NP_001721.2. NM_001730.4. [Q13887-1]
UniGeneHs.508234.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EBTNMR-A365-457[»]
ProteinModelPortalQ13887.
SMRQ13887. Positions 363-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107153. 27 interactions.
IntActQ13887. 2 interactions.
MINTMINT-3028757.
STRING9606.ENSP00000366915.

Chemistry

BindingDBQ13887.

PTM databases

PhosphoSiteQ13887.

Polymorphism databases

DMDM12644412.

Proteomic databases

MaxQBQ13887.
PaxDbQ13887.
PRIDEQ13887.

Protocols and materials databases

DNASU688.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000377687; ENSP00000366915; ENSG00000102554. [Q13887-1]
GeneID688.
KEGGhsa:688.
UCSCuc001vjd.3. human. [Q13887-1]

Organism-specific databases

CTD688.
GeneCardsGC13P073629.
HGNCHGNC:6349. KLF5.
HPAHPA040398.
HPA044231.
MIM602903. gene.
neXtProtNX_Q13887.
PharmGKBPA30139.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000261607.
HOVERGENHBG003474.
InParanoidQ13887.
KOK09206.
OMAMPNQFLQ.
OrthoDBEOG747PJ4.
PhylomeDBQ13887.
TreeFamTF350556.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
SignaLinkQ13887.

Gene expression databases

ArrayExpressQ13887.
BgeeQ13887.
CleanExHS_CKLF.
HS_KLF5.
GenevestigatorQ13887.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKLF5. human.
EvolutionaryTraceQ13887.
GeneWikiKLF5.
GenomeRNAi688.
NextBio2832.
PROQ13887.
SOURCESearch...

Entry information

Entry nameKLF5_HUMAN
AccessionPrimary (citable) accession number: Q13887
Secondary accession number(s): L0R3U5, L0R4T9, Q9UHP8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM