Reviewed, UniProtKB/Swiss-Prot Q13887 (KLF5_HUMAN)

Names and origin

Protein names

Recommended name:
    Krueppel-like factor 5
Alternative name(s):
    Intestinal-enriched krueppel-like factor
    Colon krueppel-like factor
    Transcription factor BTEB2
    Basic transcription element-binding protein 2
      Short name=BTE-binding protein 2
    GC-box-binding protein 2

Gene names

Name:	KLF5
Synonyms:	BTEB2, CKLF, IKLF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Subcellular location	Nucleus.
Tissue specificity	Expressed only in testis and placenta.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Ref.5 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA polymerase II transcription factor activity Ref.5 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 457

457

Krueppel-like factor 5

PRO_0000047169

Regions

Zinc finger

373 – 397

25

C2H2-type 1

Zinc finger

403 – 427

25

C2H2-type 2

Zinc finger

433 – 455

23

C2H2-type 3

Natural variations

Natural variant

301

1

P → S in a colorectal cancer sample; somatic mutation. Ref.7

VAR_035555

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

457

Helix Strand Turn

Details...

Beta strand

377 – 379

3

Beta strand

383 – 385

3

Helix

387 – 397

11

Beta strand

407 – 409

3

Beta strand

413 – 416

4

Helix

417 – 427

11

Beta strand

436 – 438

3

Helix

445 – 455

11

Sequences

	Sequence		Length	Mass (Da)
	Q13887-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 3BF12BE272716E57	FASTA	457	50,792
	10 20 30 40 50 60 MATRVLSMSA RLGPVPQPPA PQDEPVFAQL KPVLGAANPA RDAALFPGEE LKHAHHRPQA 70 80 90 100 110 120 QPAPAQAPQP AQPPATGPRL PPEDLVQTRC EMEKYLTPQL PPVPIIPEHK KYRRDSASVV 130 140 150 160 170 180 DQFFTDTEGL PYSINMNVFL PDITHLRTGL YKSQRPCVTH IKTEPVAIFS HQSETTAPPP 190 200 210 220 230 240 APTQALPEFT SIFSSHQTAA PEVNNIFIKQ ELPTPDLHLS VPTQQGHLYQ LLNTPDLDMP 250 260 270 280 290 300 SSTNQTAAMD TLNVSMSAAM AGLNTHTSAV PQTAVKQFQG MPPCTYTMPS QFLPQQATYF 310 320 330 340 350 360 PPSPPSSEPG SPDRQAEMLQ NLTPPPSYAA TIASKLAIHN PNLPTTLPVN SQNIQPVRYN 370 380 390 400 410 420 RRSNPDLEKR RIHYCDYPGC TKVYTKSSHL KAHLRTHTGE KPYKCTWEGC DWRFARSDEL 430 440 450 TRHYRKHTGA KPFQCGVCNR SFSRSDHLAL HMKRHQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a gene encoding human Kruppel-like factor 5 (IKLF): binding to the CAAT/GT box of the mouse lactoferrin gene promoter." Shi H., Zhang Z., Wang X., Liu S., Teng C.T. Nucleic Acids Res. 27:4807-4815(1999) [PubMed: 10572182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human basic transcription element binding protein 2." Ohnishi S., Yoshida T., Ramirez F., Terada M., Friedrich L. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Human Kruppel like factor5/KLF5: expression in human skin and hair follicles." Sur I., Unden A.-B., Toftgard R. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Placenta.
[5]	"cDNA cloning and transcriptional properties of a novel GC box-binding protein, BTEB2." Sogawa K., Imataka H., Yamasaki Y., Kusume H., Abe H., Fujii-Kuriyama Y. Nucleic Acids Res. 21:1527-1532(1993) [PubMed: 8479902] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 239-457. Tissue: Placenta.
[6]	"Solution structure of three tandem repeats of ZF-C2H2 domains from human kruppel-like factor 5." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 363-457.
[7]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-301.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF132818 mRNA. Translation: AAF18307.1.
AB030824 mRNA. Translation: BAA96461.1.
AF287272 mRNA. Translation: AAF88068.1.
BC007695 mRNA. Translation: AAH07695.1.
BC042131 mRNA. Translation: AAH42131.1.
D14520 mRNA. Translation: BAA03393.1.

IPI

IPI00015934.

PIR

S35643.

RefSeq

NP_001721.2.

UniGene

Hs.508234

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EBT	NMR	-	A	365-457	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q13887.

Proteomic databases

PRIDE

Q13887.

Genome annotation databases

Ensembl

ENSG00000102554. Homo sapiens. [Contig view]

GeneID

688.

KEGG

hsa:688.

Organism-specific databases

GeneCards

GC13P072530.

H-InvDB

HIX0011366.

HGNC

HGNC:6349. KLF5.

MIM

602903. gene.

PharmGKB

PA30139.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q13887.

HOVERGEN

Q13887.

OMA

Q13887. LQNLTPP.

Gene expression databases

ArrayExpress

Q13887.

Bgee

Q13887.

CleanEx

HS_CKLF.
HS_KLF5.

GermOnline

ENSG00000102554. Homo sapiens.

Family and domain databases

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.

Pfam

PF00096. zf-C2H2. 3 hits.
[Graphical view]

ProDom

PD000003. Znf_C2H2. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00355. ZnF_C2H2. 3 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

2832.

SOURCE

Search...

Entry information

Entry name

KLF5_HUMAN

Accession

Primary (citable) accession number: Q13887
Secondary accession number(s): Q9UHP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)