Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bleomycin hydrolase

Gene

BLMH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.By similarity

Catalytic activityi

Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei731
Active sitei3721
Active sitei3961

GO - Molecular functioni

  • aminopeptidase activity Source: Reactome
  • carboxypeptidase activity Source: ProtInc
  • cysteine-type endopeptidase activity Source: ProtInc
  • cysteine-type peptidase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciZFISH:HS03127-MONOMER.
BRENDAi3.4.22.40. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SABIO-RKQ13867.

Protein family/group databases

MEROPSiC01.084.

Names & Taxonomyi

Protein namesi
Recommended name:
Bleomycin hydrolase (EC:3.4.22.40)
Short name:
BH
Short name:
BLM hydrolase
Short name:
BMH
Gene namesi
Name:BLMH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:1059. BLMH.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi642.
MalaCardsiBLMH.
OpenTargetsiENSG00000108578.
Orphaneti364195. Resistance to bleomycine in the treatment of testicular cancer.
PharmGKBiPA25370.

Chemistry databases

DrugBankiDB00290. Bleomycin.

Polymorphism and mutation databases

BioMutaiBLMH.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000505501 – 455Bleomycin hydrolaseAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei391N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ13867.
MaxQBiQ13867.
PaxDbiQ13867.
PeptideAtlasiQ13867.
PRIDEiQ13867.

PTM databases

iPTMnetiQ13867.
PhosphoSitePlusiQ13867.

Expressioni

Gene expression databases

BgeeiENSG00000108578.
CleanExiHS_BLMH.
ExpressionAtlasiQ13867. baseline and differential.
GenevisibleiQ13867. HS.

Organism-specific databases

HPAiHPA039548.
HPA064307.

Interactioni

Subunit structurei

Homohexamer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-718504,EBI-718504
APPP05067-42EBI-718504,EBI-302641
NUDT12Q9BQG23EBI-718504,EBI-10230612
WDYHV1Q96HA83EBI-718504,EBI-741158

Protein-protein interaction databases

BioGridi107111. 56 interactors.
IntActiQ13867. 44 interactors.
MINTiMINT-1397729.
STRINGi9606.ENSP00000261714.

Structurei

Secondary structure

1455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi8 – 19Combined sources12
Helixi22 – 31Combined sources10
Helixi36 – 40Combined sources5
Helixi43 – 48Combined sources6
Beta strandi54 – 56Combined sources3
Beta strandi69 – 71Combined sources3
Helixi73 – 89Combined sources17
Helixi99 – 123Combined sources25
Helixi131 – 138Combined sources8
Helixi147 – 157Combined sources11
Helixi162 – 164Combined sources3
Helixi170 – 172Combined sources3
Helixi175 – 197Combined sources23
Helixi202 – 224Combined sources23
Beta strandi229 – 236Combined sources8
Beta strandi242 – 248Combined sources7
Helixi250 – 257Combined sources8
Turni258 – 261Combined sources4
Helixi264 – 266Combined sources3
Beta strandi267 – 271Combined sources5
Beta strandi280 – 286Combined sources7
Beta strandi300 – 302Combined sources3
Helixi305 – 317Combined sources13
Beta strandi322 – 326Combined sources5
Turni328 – 331Combined sources4
Turni334 – 337Combined sources4
Helixi346 – 350Combined sources5
Helixi359 – 364Combined sources6
Beta strandi372 – 381Combined sources10
Beta strandi389 – 395Combined sources7
Beta strandi407 – 411Combined sources5
Helixi412 – 418Combined sources7
Beta strandi419 – 425Combined sources7
Helixi426 – 428Combined sources3
Helixi431 – 434Combined sources4
Helixi435 – 438Combined sources4
Beta strandi442 – 444Combined sources3
Helixi449 – 451Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CB5X-ray2.59A/B/C2-454[»]
2CB5X-ray1.85A/B2-454[»]
ProteinModelPortaliQ13867.
SMRiQ13867.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13867.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4128. Eukaryota.
COG3579. LUCA.
GeneTreeiENSGT00390000001735.
HOGENOMiHOG000064089.
HOVERGENiHBG002388.
InParanoidiQ13867.
KOiK01372.
OMAiKLYTVDY.
OrthoDBiEOG091G07WT.
PhylomeDBiQ13867.
TreeFamiTF323372.

Family and domain databases

CDDicd00585. Peptidase_C1B. 1 hit.
InterProiIPR000169. Pept_cys_AS.
IPR004134. Peptidase_C1B.
[Graphical view]
PANTHERiPTHR10363. PTHR10363. 1 hit.
PfamiPF03051. Peptidase_C1_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005700. PepC. 1 hit.
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ
60 70 80 90 100
HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS
110 120 130 140 150
YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML
160 170 180 190 200
VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA
210 220 230 240 250
TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP
260 270 280 290 300
LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY
310 320 330 340 350
NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF
360 370 380 390 400
GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE
410 420 430 440 450
DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM

GALAE
Length:455
Mass (Da):52,562
Last modified:November 1, 1996 - v1
Checksum:i577E86241EB0D460
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_010896443I → V Common polymorphism. 2 PublicationsCorresponds to variant rs1050565dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92106 mRNA. Translation: CAA63078.1.
BT007018 mRNA. Translation: AAP35664.1.
AK223403 mRNA. Translation: BAD97123.1.
AK312896 mRNA. Translation: BAG35743.1.
CH471159 Genomic DNA. Translation: EAW51224.1.
BC003616 mRNA. Translation: AAH03616.1.
AF091082 mRNA. Translation: AAC72951.1.
CCDSiCCDS32604.1.
RefSeqiNP_000377.1. NM_000386.3.
UniGeneiHs.371914.

Genome annotation databases

EnsembliENST00000261714; ENSP00000261714; ENSG00000108578.
GeneIDi642.
KEGGihsa:642.
UCSCiuc002hez.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92106 mRNA. Translation: CAA63078.1.
BT007018 mRNA. Translation: AAP35664.1.
AK223403 mRNA. Translation: BAD97123.1.
AK312896 mRNA. Translation: BAG35743.1.
CH471159 Genomic DNA. Translation: EAW51224.1.
BC003616 mRNA. Translation: AAH03616.1.
AF091082 mRNA. Translation: AAC72951.1.
CCDSiCCDS32604.1.
RefSeqiNP_000377.1. NM_000386.3.
UniGeneiHs.371914.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CB5X-ray2.59A/B/C2-454[»]
2CB5X-ray1.85A/B2-454[»]
ProteinModelPortaliQ13867.
SMRiQ13867.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107111. 56 interactors.
IntActiQ13867. 44 interactors.
MINTiMINT-1397729.
STRINGi9606.ENSP00000261714.

Chemistry databases

DrugBankiDB00290. Bleomycin.

Protein family/group databases

MEROPSiC01.084.

PTM databases

iPTMnetiQ13867.
PhosphoSitePlusiQ13867.

Polymorphism and mutation databases

BioMutaiBLMH.

Proteomic databases

EPDiQ13867.
MaxQBiQ13867.
PaxDbiQ13867.
PeptideAtlasiQ13867.
PRIDEiQ13867.

Protocols and materials databases

DNASUi642.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261714; ENSP00000261714; ENSG00000108578.
GeneIDi642.
KEGGihsa:642.
UCSCiuc002hez.3. human.

Organism-specific databases

CTDi642.
DisGeNETi642.
GeneCardsiBLMH.
HGNCiHGNC:1059. BLMH.
HPAiHPA039548.
HPA064307.
MalaCardsiBLMH.
MIMi602403. gene.
neXtProtiNX_Q13867.
OpenTargetsiENSG00000108578.
Orphaneti364195. Resistance to bleomycine in the treatment of testicular cancer.
PharmGKBiPA25370.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4128. Eukaryota.
COG3579. LUCA.
GeneTreeiENSGT00390000001735.
HOGENOMiHOG000064089.
HOVERGENiHBG002388.
InParanoidiQ13867.
KOiK01372.
OMAiKLYTVDY.
OrthoDBiEOG091G07WT.
PhylomeDBiQ13867.
TreeFamiTF323372.

Enzyme and pathway databases

BioCyciZFISH:HS03127-MONOMER.
BRENDAi3.4.22.40. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SABIO-RKQ13867.

Miscellaneous databases

ChiTaRSiBLMH. human.
EvolutionaryTraceiQ13867.
GeneWikiiBleomycin_hydrolase.
BLMH.
GenomeRNAii642.
PROiQ13867.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108578.
CleanExiHS_BLMH.
ExpressionAtlasiQ13867. baseline and differential.
GenevisibleiQ13867. HS.

Family and domain databases

CDDicd00585. Peptidase_C1B. 1 hit.
InterProiIPR000169. Pept_cys_AS.
IPR004134. Peptidase_C1B.
[Graphical view]
PANTHERiPTHR10363. PTHR10363. 1 hit.
PfamiPF03051. Peptidase_C1_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005700. PepC. 1 hit.
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLMH_HUMAN
AccessioniPrimary (citable) accession number: Q13867
Secondary accession number(s): B2R796, Q53F86, Q9UER9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.