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Protein

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

Gene

TNFAIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • GTP-Rho binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell migration Source: UniProtKB
  • DNA replication Source: UniProtKB
  • immune response Source: UniProtKB
  • negative regulation of Rho protein signal transduction Source: UniProtKB
  • positive regulation of DNA replication Source: Ensembl
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein homooligomerization Source: InterPro
  • protein ubiquitination Source: UniProtKB
  • stress fiber assembly Source: UniProtKB

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

SIGNORiQ13829
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
Short name:
hBACURD2
Alternative name(s):
BTB/POZ domain-containing protein TNFAIP1
Protein B12
Tumor necrosis factor, alpha-induced protein 1, endothelial
Gene namesi
Name:TNFAIP1
Synonyms:BACURD2, EDP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000109079.9
HGNCiHGNC:11894 TNFAIP1
MIMi191161 gene
neXtProtiNX_Q13829

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71 – 73ILI → AAA: Abolishes interaction with CUL3 and induces abnormal actin stress fibers. 1 Publication3
Mutagenesisi142S → A: Does not affect phosphorylation level; when associated with A-237. 1 Publication1
Mutagenesisi237T → A: Does not affect phosphorylation level; when associated with A-142. 1 Publication1
Mutagenesisi265S → A: Does not affect phosphorylation level. 1 Publication1
Mutagenesisi278S → A: Slightly impairs phosphorylation level. 1 Publication1
Mutagenesisi280S → A: Strongly impairs phosphorylation level. 1 Publication1

Organism-specific databases

DisGeNETi7126
OpenTargetsiENSG00000109079
PharmGKBiPA36591

Polymorphism and mutation databases

BioMutaiTNFAIP1
DMDMi2833248

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913001 – 316BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2Add BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278PhosphoserineCombined sources1
Modified residuei280Phosphoserine; by CK2Combined sources1 Publication1

Post-translational modificationi

Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13829
MaxQBiQ13829
PaxDbiQ13829
PeptideAtlasiQ13829
PRIDEiQ13829

PTM databases

iPTMnetiQ13829
PhosphoSitePlusiQ13829

Expressioni

Inductioni

By TNF, IL1B/interleukin-1 beta and bacterial lipopolysaccharides (LPS).

Gene expression databases

BgeeiENSG00000109079
CleanExiHS_TNFAIP1
ExpressionAtlasiQ13829 baseline and differential
GenevisibleiQ13829 HS

Organism-specific databases

HPAiHPA013333
HPA014641

Interactioni

Subunit structurei

Component of the BCR(TNFAIP1) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • GTP-Rho binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112981, 59 interactors
IntActiQ13829, 45 interactors
MINTiQ13829
STRINGi9606.ENSP00000226225

Structurei

3D structure databases

ProteinModelPortaliQ13829
SMRiQ13829
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 96BTBPROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the BACURD family.Curated

Phylogenomic databases

eggNOGiKOG2716 Eukaryota
ENOG410XQYZ LUCA
GeneTreeiENSGT00900000140932
HOGENOMiHOG000294088
HOVERGENiHBG052219
InParanoidiQ13829
KOiK15074
OMAiREMSGDT
OrthoDBiEOG091G0L1Y
PhylomeDBiQ13829
TreeFamiTF315649

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR003131 T1-type_BTB
PfamiView protein in Pfam
PF02214 BTB_2, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13829-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGDTCLCPA SGAKPKLSGF KGGGLGNKYV QLNVGGSLYY TTVRALTRHD
60 70 80 90 100
TMLKAMFSGR MEVLTDKEGW ILIDRCGKHF GTILNYLRDD TITLPQNRQE
110 120 130 140 150
IKELMAEAKY YLIQGLVNMC QSALQDKKDS YQPVCNIPII TSLKEEERLI
160 170 180 190 200
ESSTKPVVKL LYNRSNNKYS YTSNSDDHLL KNIELFDKLS LRFNGRVLFI
210 220 230 240 250
KDVIGDEICC WSFYGQGRKL AEVCCTSIVY ATEKKQTKVE FPEARIYEET
260 270 280 290 300
LNVLLYETPR VPDNSLLEAT SRSRSQASPS EDEETFELRD RVRRIHVKRY
310
STYDDRQLGH QSTHRD
Length:316
Mass (Da):36,204
Last modified:November 1, 1996 - v1
Checksum:iD20B810A00507DCF
GO
Isoform 2 (identifier: Q13829-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Note: No experimental confirmation available.
Show »
Length:212
Mass (Da):24,709
Checksum:i812B6C0368AA6908
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560291 – 104Missing in isoform 2. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80783 mRNA Translation: AAA58385.1
AK300584 mRNA Translation: BAH13310.1
AK312387 mRNA Translation: BAG35304.1
CR456819 mRNA Translation: CAG33100.1
BT020121 mRNA Translation: AAV38924.1
AY065346 Genomic DNA Translation: AAL38649.1
AC002094 Genomic DNA No translation available.
CH471159 Genomic DNA Translation: EAW51077.1
BC001643 mRNA Translation: AAH01643.1
BC001949 mRNA Translation: AAH01949.1
CCDSiCCDS11227.1 [Q13829-1]
RefSeqiNP_066960.1, NM_021137.4 [Q13829-1]
UniGeneiHs.76090

Genome annotation databases

EnsembliENST00000226225; ENSP00000226225; ENSG00000109079 [Q13829-1]
ENST00000544907; ENSP00000440749; ENSG00000109079 [Q13829-2]
GeneIDi7126
KEGGihsa:7126
UCSCiuc002hay.4 human [Q13829-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBACD2_HUMAN
AccessioniPrimary (citable) accession number: Q13829
Secondary accession number(s): B7Z6M4, Q5TZQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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