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Q13829

- BACD2_HUMAN

UniProt

Q13829 - BACD2_HUMAN

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Protein
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
Gene
TNFAIP1, BACURD2, EDP1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.2 Publications

Pathwayi

GO - Molecular functioni

  1. GTP-Rho binding Source: UniProtKB
  2. protein binding Source: IntAct

GO - Biological processi

  1. DNA replication Source: UniProtKB
  2. apoptotic process Source: UniProtKB
  3. cell migration Source: UniProtKB
  4. embryo development Source: UniProtKB
  5. immune response Source: UniProtKB
  6. negative regulation of Rho protein signal transduction Source: UniProtKB
  7. positive regulation of DNA replication Source: Ensembl
  8. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  9. protein homooligomerization Source: InterPro
  10. protein ubiquitination Source: UniProtKB
  11. stress fiber assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
Short name:
hBACURD2
Alternative name(s):
BTB/POZ domain-containing protein TNFAIP1
Protein B12
Tumor necrosis factor, alpha-induced protein 1, endothelial
Gene namesi
Name:TNFAIP1
Synonyms:BACURD2, EDP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:11894. TNFAIP1.

Subcellular locationi

Cytoplasm. Nucleus. Endosome
Note: Colocalizes with RHOB in endosomes.2 Publications

GO - Cellular componenti

  1. Cul3-RING ubiquitin ligase complex Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. endosome Source: UniProtKB
  4. nucleolus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 733ILI → AAA: Abolishes interaction with CUL3 and induces abnormal actin stress fibers. 1 Publication
Mutagenesisi142 – 1421S → A: Does not affect phosphorylation level; when associated with A-237. 1 Publication
Mutagenesisi237 – 2371T → A: Does not affect phosphorylation level; when associated with A-142. 1 Publication
Mutagenesisi265 – 2651S → A: Does not affect phosphorylation level. 1 Publication
Mutagenesisi278 – 2781S → A: Slightly impairs phosphorylation level. 1 Publication
Mutagenesisi280 – 2801S → A: Strongly impairs phosphorylation level. 1 Publication

Organism-specific databases

PharmGKBiPA36591.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
PRO_0000191300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei278 – 2781Phosphoserine2 Publications
Modified residuei280 – 2801Phosphoserine; by CK22 Publications

Post-translational modificationi

Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ13829.
PaxDbiQ13829.
PRIDEiQ13829.

PTM databases

PhosphoSiteiQ13829.

Expressioni

Inductioni

By TNF, IL1B/interleukin-1 beta and bacterial lipopolysaccharides (LPS).

Gene expression databases

ArrayExpressiQ13829.
BgeeiQ13829.
CleanExiHS_TNFAIP1.
GenevestigatoriQ13829.

Organism-specific databases

HPAiHPA013333.
HPA014641.

Interactioni

Subunit structurei

Component of the BCR(BACURD2) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHOBP627455EBI-2505861,EBI-602647

Protein-protein interaction databases

BioGridi112981. 14 interactions.
IntActiQ13829. 8 interactions.
MINTiMINT-3028741.
STRINGi9606.ENSP00000226225.

Structurei

3D structure databases

ProteinModelPortaliQ13829.
SMRiQ13829. Positions 27-147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 9669BTB
Add
BLAST

Sequence similaritiesi

Belongs to the BACURD family.
Contains 1 BTB (POZ) domain.

Phylogenomic databases

eggNOGiNOG257745.
HOGENOMiHOG000294088.
HOVERGENiHBG052219.
InParanoidiQ13829.
KOiK15074.
OMAiEMSGDTC.
PhylomeDBiQ13829.
TreeFamiTF315649.

Family and domain databases

Gene3Di3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR003131. T1-type_BTB.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13829-1 [UniParc]FASTAAdd to Basket

« Hide

MSGDTCLCPA SGAKPKLSGF KGGGLGNKYV QLNVGGSLYY TTVRALTRHD    50
TMLKAMFSGR MEVLTDKEGW ILIDRCGKHF GTILNYLRDD TITLPQNRQE 100
IKELMAEAKY YLIQGLVNMC QSALQDKKDS YQPVCNIPII TSLKEEERLI 150
ESSTKPVVKL LYNRSNNKYS YTSNSDDHLL KNIELFDKLS LRFNGRVLFI 200
KDVIGDEICC WSFYGQGRKL AEVCCTSIVY ATEKKQTKVE FPEARIYEET 250
LNVLLYETPR VPDNSLLEAT SRSRSQASPS EDEETFELRD RVRRIHVKRY 300
STYDDRQLGH QSTHRD 316
Length:316
Mass (Da):36,204
Last modified:November 1, 1996 - v1
Checksum:iD20B810A00507DCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80783 mRNA. Translation: AAA58385.1.
AK312387 mRNA. Translation: BAG35304.1.
CR456819 mRNA. Translation: CAG33100.1.
BT020121 mRNA. Translation: AAV38924.1.
AY065346 Genomic DNA. Translation: AAL38649.1.
AC002094 Genomic DNA. No translation available.
CH471159 Genomic DNA. Translation: EAW51077.1.
BC001643 mRNA. Translation: AAH01643.1.
BC001949 mRNA. Translation: AAH01949.1.
CCDSiCCDS11227.1.
RefSeqiNP_066960.1. NM_021137.4.
UniGeneiHs.76090.

Genome annotation databases

EnsembliENST00000226225; ENSP00000226225; ENSG00000109079.
ENST00000576850; ENSP00000459080; ENSG00000262447.
GeneIDi7126.
KEGGihsa:7126.
UCSCiuc002hax.2. human.

Polymorphism databases

DMDMi2833248.

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80783 mRNA. Translation: AAA58385.1 .
AK312387 mRNA. Translation: BAG35304.1 .
CR456819 mRNA. Translation: CAG33100.1 .
BT020121 mRNA. Translation: AAV38924.1 .
AY065346 Genomic DNA. Translation: AAL38649.1 .
AC002094 Genomic DNA. No translation available.
CH471159 Genomic DNA. Translation: EAW51077.1 .
BC001643 mRNA. Translation: AAH01643.1 .
BC001949 mRNA. Translation: AAH01949.1 .
CCDSi CCDS11227.1.
RefSeqi NP_066960.1. NM_021137.4.
UniGenei Hs.76090.

3D structure databases

ProteinModelPortali Q13829.
SMRi Q13829. Positions 27-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112981. 14 interactions.
IntActi Q13829. 8 interactions.
MINTi MINT-3028741.
STRINGi 9606.ENSP00000226225.

PTM databases

PhosphoSitei Q13829.

Polymorphism databases

DMDMi 2833248.

Proteomic databases

MaxQBi Q13829.
PaxDbi Q13829.
PRIDEi Q13829.

Protocols and materials databases

DNASUi 7126.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000226225 ; ENSP00000226225 ; ENSG00000109079 .
ENST00000576850 ; ENSP00000459080 ; ENSG00000262447 .
GeneIDi 7126.
KEGGi hsa:7126.
UCSCi uc002hax.2. human.

Organism-specific databases

CTDi 7126.
GeneCardsi GC17P026662.
HGNCi HGNC:11894. TNFAIP1.
HPAi HPA013333.
HPA014641.
MIMi 191161. gene.
neXtProti NX_Q13829.
PharmGKBi PA36591.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG257745.
HOGENOMi HOG000294088.
HOVERGENi HBG052219.
InParanoidi Q13829.
KOi K15074.
OMAi EMSGDTC.
PhylomeDBi Q13829.
TreeFami TF315649.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

GeneWikii TNFAIP1.
GenomeRNAii 7126.
NextBioi 27887.
PROi Q13829.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q13829.
Bgeei Q13829.
CleanExi HS_TNFAIP1.
Genevestigatori Q13829.

Family and domain databases

Gene3Di 3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR003131. T1-type_BTB.
[Graphical view ]
Pfami PF02214. BTB_2. 1 hit.
[Graphical view ]
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
PROSITEi PS50097. BTB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel tumor necrosis factor-alpha-induced endothelial primary response gene."
    Wolf F.W., Marks R.M., Sarma V., Byers M.G., Katz R.W., Shows T.B., Dixit V.M.
    J. Biol. Chem. 267:1317-1326(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Endothelial cell.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. SeattleSNPs variation discovery resource
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278 AND SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells."
    Kim D.M., Chung K.S., Choi S.J., Jung Y.J., Park S.K., Han G.H., Ha J.S., Song K.B., Choi N.S., Kim H.M., Won M., Seo Y.S.
    Int. J. Cancer 125:2520-2527(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RHOB.
  11. "CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA."
    Yang L., Liu N., Hu X., Zhang W., Wang T., Li H., Zhang B., Xiang S., Zhou J., Zhang J.
    Mol. Biol. Rep. 37:2967-2973(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CSNK2B AND PCNA, PHOSPHORYLATION AT SER-280, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-142; THR-237; SER-265; SER-278 AND SER-280.
  12. "Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement."
    Chen Y., Yang Z., Meng M., Zhao Y., Dong N., Yan H., Liu L., Ding M., Peng H.B., Shao F.
    Mol. Cell 35:841-855(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A BCR (BTB-CUL3-RBX1) E3 UBIQUITIN LIGASE COMPLEX, INTERACTION WITH RHOA, MUTAGENESIS OF 71-ILE--ILE-73.
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiBACD2_HUMAN
AccessioniPrimary (citable) accession number: Q13829
Secondary accession number(s): Q5TZQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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