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Q13829

- BACD2_HUMAN

UniProt

Q13829 - BACD2_HUMAN

Protein

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

Gene

TNFAIP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. GTP-Rho binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. apoptotic process Source: UniProtKB
    2. cell migration Source: UniProtKB
    3. DNA replication Source: UniProtKB
    4. embryo development Source: UniProtKB
    5. immune response Source: UniProtKB
    6. negative regulation of Rho protein signal transduction Source: UniProtKB
    7. positive regulation of DNA replication Source: Ensembl
    8. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
    9. protein homooligomerization Source: InterPro
    10. protein ubiquitination Source: UniProtKB
    11. stress fiber assembly Source: UniProtKB

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
    Short name:
    hBACURD2
    Alternative name(s):
    BTB/POZ domain-containing protein TNFAIP1
    Protein B12
    Tumor necrosis factor, alpha-induced protein 1, endothelial
    Gene namesi
    Name:TNFAIP1
    Synonyms:BACURD2, EDP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:11894. TNFAIP1.

    Subcellular locationi

    Cytoplasm. Nucleus. Endosome
    Note: Colocalizes with RHOB in endosomes.

    GO - Cellular componenti

    1. Cul3-RING ubiquitin ligase complex Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. endosome Source: UniProtKB
    4. nucleolus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi71 – 733ILI → AAA: Abolishes interaction with CUL3 and induces abnormal actin stress fibers. 1 Publication
    Mutagenesisi142 – 1421S → A: Does not affect phosphorylation level; when associated with A-237. 2 Publications
    Mutagenesisi237 – 2371T → A: Does not affect phosphorylation level; when associated with A-142. 2 Publications
    Mutagenesisi265 – 2651S → A: Does not affect phosphorylation level. 2 Publications
    Mutagenesisi278 – 2781S → A: Slightly impairs phosphorylation level. 2 Publications
    Mutagenesisi280 – 2801S → A: Strongly impairs phosphorylation level. 2 Publications

    Organism-specific databases

    PharmGKBiPA36591.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2PRO_0000191300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei278 – 2781Phosphoserine2 Publications
    Modified residuei280 – 2801Phosphoserine; by CK22 Publications

    Post-translational modificationi

    Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ13829.
    PaxDbiQ13829.
    PRIDEiQ13829.

    PTM databases

    PhosphoSiteiQ13829.

    Expressioni

    Inductioni

    By TNF, IL1B/interleukin-1 beta and bacterial lipopolysaccharides (LPS).

    Gene expression databases

    ArrayExpressiQ13829.
    BgeeiQ13829.
    CleanExiHS_TNFAIP1.
    GenevestigatoriQ13829.

    Organism-specific databases

    HPAiHPA013333.
    HPA014641.

    Interactioni

    Subunit structurei

    Component of the BCR(BACURD2) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RHOBP627455EBI-2505861,EBI-602647

    Protein-protein interaction databases

    BioGridi112981. 14 interactions.
    IntActiQ13829. 8 interactions.
    MINTiMINT-3028741.
    STRINGi9606.ENSP00000226225.

    Structurei

    3D structure databases

    ProteinModelPortaliQ13829.
    SMRiQ13829. Positions 27-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 9669BTBPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the BACURD family.Curated
    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG257745.
    HOGENOMiHOG000294088.
    HOVERGENiHBG052219.
    InParanoidiQ13829.
    KOiK15074.
    OMAiEMSGDTC.
    PhylomeDBiQ13829.
    TreeFamiTF315649.

    Family and domain databases

    Gene3Di3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR003131. T1-type_BTB.
    [Graphical view]
    PfamiPF02214. BTB_2. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q13829-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGDTCLCPA SGAKPKLSGF KGGGLGNKYV QLNVGGSLYY TTVRALTRHD    50
    TMLKAMFSGR MEVLTDKEGW ILIDRCGKHF GTILNYLRDD TITLPQNRQE 100
    IKELMAEAKY YLIQGLVNMC QSALQDKKDS YQPVCNIPII TSLKEEERLI 150
    ESSTKPVVKL LYNRSNNKYS YTSNSDDHLL KNIELFDKLS LRFNGRVLFI 200
    KDVIGDEICC WSFYGQGRKL AEVCCTSIVY ATEKKQTKVE FPEARIYEET 250
    LNVLLYETPR VPDNSLLEAT SRSRSQASPS EDEETFELRD RVRRIHVKRY 300
    STYDDRQLGH QSTHRD 316
    Length:316
    Mass (Da):36,204
    Last modified:November 1, 1996 - v1
    Checksum:iD20B810A00507DCF
    GO
    Isoform 2 (identifier: Q13829-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-104: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:212
    Mass (Da):24,709
    Checksum:i812B6C0368AA6908
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 104104Missing in isoform 2. 1 PublicationVSP_056029Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80783 mRNA. Translation: AAA58385.1.
    AK300584 mRNA. Translation: BAH13310.1.
    AK312387 mRNA. Translation: BAG35304.1.
    CR456819 mRNA. Translation: CAG33100.1.
    BT020121 mRNA. Translation: AAV38924.1.
    AY065346 Genomic DNA. Translation: AAL38649.1.
    AC002094 Genomic DNA. No translation available.
    CH471159 Genomic DNA. Translation: EAW51077.1.
    BC001643 mRNA. Translation: AAH01643.1.
    BC001949 mRNA. Translation: AAH01949.1.
    CCDSiCCDS11227.1.
    RefSeqiNP_066960.1. NM_021137.4.
    UniGeneiHs.76090.

    Genome annotation databases

    EnsembliENST00000226225; ENSP00000226225; ENSG00000109079.
    ENST00000544907; ENSP00000440749; ENSG00000109079.
    GeneIDi7126.
    KEGGihsa:7126.
    UCSCiuc002hax.2. human.

    Polymorphism databases

    DMDMi2833248.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80783 mRNA. Translation: AAA58385.1 .
    AK300584 mRNA. Translation: BAH13310.1 .
    AK312387 mRNA. Translation: BAG35304.1 .
    CR456819 mRNA. Translation: CAG33100.1 .
    BT020121 mRNA. Translation: AAV38924.1 .
    AY065346 Genomic DNA. Translation: AAL38649.1 .
    AC002094 Genomic DNA. No translation available.
    CH471159 Genomic DNA. Translation: EAW51077.1 .
    BC001643 mRNA. Translation: AAH01643.1 .
    BC001949 mRNA. Translation: AAH01949.1 .
    CCDSi CCDS11227.1.
    RefSeqi NP_066960.1. NM_021137.4.
    UniGenei Hs.76090.

    3D structure databases

    ProteinModelPortali Q13829.
    SMRi Q13829. Positions 27-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112981. 14 interactions.
    IntActi Q13829. 8 interactions.
    MINTi MINT-3028741.
    STRINGi 9606.ENSP00000226225.

    PTM databases

    PhosphoSitei Q13829.

    Polymorphism databases

    DMDMi 2833248.

    Proteomic databases

    MaxQBi Q13829.
    PaxDbi Q13829.
    PRIDEi Q13829.

    Protocols and materials databases

    DNASUi 7126.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000226225 ; ENSP00000226225 ; ENSG00000109079 .
    ENST00000544907 ; ENSP00000440749 ; ENSG00000109079 .
    GeneIDi 7126.
    KEGGi hsa:7126.
    UCSCi uc002hax.2. human.

    Organism-specific databases

    CTDi 7126.
    GeneCardsi GC17P026662.
    HGNCi HGNC:11894. TNFAIP1.
    HPAi HPA013333.
    HPA014641.
    MIMi 191161. gene.
    neXtProti NX_Q13829.
    PharmGKBi PA36591.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG257745.
    HOGENOMi HOG000294088.
    HOVERGENi HBG052219.
    InParanoidi Q13829.
    KOi K15074.
    OMAi EMSGDTC.
    PhylomeDBi Q13829.
    TreeFami TF315649.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    GeneWikii TNFAIP1.
    GenomeRNAii 7126.
    NextBioi 27887.
    PROi Q13829.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13829.
    Bgeei Q13829.
    CleanExi HS_TNFAIP1.
    Genevestigatori Q13829.

    Family and domain databases

    Gene3Di 3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR003131. T1-type_BTB.
    [Graphical view ]
    Pfami PF02214. BTB_2. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a novel tumor necrosis factor-alpha-induced endothelial primary response gene."
      Wolf F.W., Marks R.M., Sarma V., Byers M.G., Katz R.W., Shows T.B., Dixit V.M.
      J. Biol. Chem. 267:1317-1326(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Endothelial cell.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Amygdala.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. SeattleSNPs variation discovery resource
      Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278 AND SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells."
      Kim D.M., Chung K.S., Choi S.J., Jung Y.J., Park S.K., Han G.H., Ha J.S., Song K.B., Choi N.S., Kim H.M., Won M., Seo Y.S.
      Int. J. Cancer 125:2520-2527(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RHOB.
    11. "CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA."
      Yang L., Liu N., Hu X., Zhang W., Wang T., Li H., Zhang B., Xiang S., Zhou J., Zhang J.
      Mol. Biol. Rep. 37:2967-2973(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CSNK2B AND PCNA, PHOSPHORYLATION AT SER-280, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-142; THR-237; SER-265; SER-278 AND SER-280.
    12. "Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement."
      Chen Y., Yang Z., Meng M., Zhao Y., Dong N., Yan H., Liu L., Ding M., Peng H.B., Shao F.
      Mol. Cell 35:841-855(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN A BCR (BTB-CUL3-RBX1) E3 UBIQUITIN LIGASE COMPLEX, INTERACTION WITH RHOA, MUTAGENESIS OF 71-ILE--ILE-73.
    13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiBACD2_HUMAN
    AccessioniPrimary (citable) accession number: Q13829
    Secondary accession number(s): B7Z6M4, Q5TZQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3