Q13795 (ARFRP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor-related protein 1
Short name=ARF-related protein 1
Short name=ARP

Gene names

Name:	ARFRP1
Synonyms:	ARP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possibly involved in plasma membrane-related signaling events.
Subunit structure	Interacts with SYS1. Ref.8
Subcellular location	Golgi apparatus Ref.8.
Tissue specificity	Found in most tissues.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Cellular component	Golgi apparatus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane protein transport Inferred from mutant phenotype PubMed 16129887PubMed 19224922. Source: UniProtKB gastrulation Inferred from electronic annotation. Source: Compara protein localization to Golgi apparatus Inferred from mutant phenotype PubMed 19224922. Source: UniProtKB retrograde transport, endosome to Golgi Inferred from mutant phenotype PubMed 16129887. Source: UniProtKB signal transduction Traceable author statement Ref.1. Source: ProtInc small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 19224922. Source: UniProtKB membrane Traceable author statement Ref.1. Source: ProtInc trans-Golgi network Inferred from direct assay PubMed 16129887. Source: UniProtKB
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q13795-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q13795-2)

The sequence of this isoform differs from the canonical sequence as follows:
     174-201: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	ADP-ribosylation factor-related protein 1		PRO_0000207487
Regions
	Nucleotide binding	24 – 31	8	GTP By similarity
	Nucleotide binding	75 – 79	5	GTP By similarity
	Nucleotide binding	134 – 137	4	GTP By similarity
Natural variations
	Alternative sequence	174 – 201	28	Missing in isoform 2.		VSP_043531
	Natural variant	108	1	L → V in a breast cancer sample; somatic mutation. Ref.9		VAR_036559

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8A75CF7A3B268158	FASTA	201	22,614
	10 20 30 40 50 60 MYTLLSGLYK YMFQKDEYCI LILGLDNAGK TTFLEQSKTR FNKNYKGMSL SKITTTVGLN 70 80 90 100 110 120 IGTVDVGKAR LMFWDLGGQE ELQSLWDKYY AECHGVIYVI DSTDEERLAE SKQAFEKVVT 130 140 150 160 170 180 SEALCGVPVL VLANKQDVET CLSIPDIKTA FSDCTSKIGR RDCLTQACSA LTGKGVREGI 190 200 EWMVKCVVRN VHRPPRQRDI T
	Isoform 2 [UniParc]. Checksum: 30A53245BE1DB13B	FASTA	173	19,272
	10 20 30 40 50 60 MYTLLSGLYK YMFQKDEYCI LILGLDNAGK TTFLEQSKTR FNKNYKGMSL SKITTTVGLN 70 80 90 100 110 120 IGTVDVGKAR LMFWDLGGQE ELQSLWDKYY AECHGVIYVI DSTDEERLAE SKQAFEKVVT 130 140 150 160 170 SEALCGVPVL VLANKQDVET CLSIPDIKTA FSDCTSKIGR RDCLTQACSA LTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ARP is a plasma membrane-associated Ras-related GTPase with remote similarity to the family of ADP-ribosylation factors." Schuermann A., Massmann S., Joost H.-G. J. Biol. Chem. 270:30657-30663(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster." Bai C., Connolly B., Metzker M.L., Hilliard C.A., Liu X., Sandig V., Soderman A., Galloway S.M., Liu Q., Austin C.P., Caskey C.T. Proc. Natl. Acad. Sci. U.S.A. 97:1230-1235(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Placenta.
[8]	"Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p." Behnia R., Panic B., Whyte J.R.C., Munro S. Nat. Cell Biol. 6:405-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SYS1.
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-108.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91504 mRNA. Translation: CAA62804.1. AF217796 Genomic DNA. Translation: AAF35246.1. CR456752 mRNA. Translation: CAG33033.1. BT019624 mRNA. Translation: AAV38430.1. BT019663 mRNA. Translation: AAV38469.1. AL121845 Genomic DNA. Translation: CAC03669.1. CH471077 Genomic DNA. Translation: EAW75231.1. CH471077 Genomic DNA. Translation: EAW75232.1. CH471077 Genomic DNA. Translation: EAW75235.1. BC092480 mRNA. Translation: AAH92480.1. BC093967 mRNA. Translation: AAH93967.1. BC143447 mRNA. Translation: AAI43448.1. BC143448 mRNA. Translation: AAI43449.1.
IPI	IPI00015178. IPI00914030.
RefSeq	NP_001128230.1. NM_001134758.2. NP_001254476.1. NM_001267547.1. NP_001254477.1. NM_001267548.1. NP_001254478.1. NM_001267549.1. NP_003215.1. NM_003224.4.
UniGene	Hs.389277.
3D structure databases
ProteinModelPortal	Q13795.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000326884.
PTM databases
PhosphoSite	Q13795.
Polymorphism databases
DMDM	2492927.
Proteomic databases
PaxDb	Q13795.
PRIDE	Q13795.
Protocols and materials databases
DNASU	10139.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000324228; ENSP00000326884; ENSG00000101246. ENST00000359715; ENSP00000352746; ENSG00000101246. ENST00000440854; ENSP00000403942; ENSG00000101246.
GeneID	10139.
KEGG	hsa:10139.
UCSC	uc002yga.3. human.
Organism-specific databases
CTD	10139.
GeneCards	GC20M062331.
HGNC	HGNC:662. ARFRP1.
HPA	HPA047028.
MIM	604699. gene.
neXtProt	NX_Q13795.
PharmGKB	PA24946.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000163691.
HOVERGEN	HBG002073.
InParanoid	Q13795.
KO	K07952.
OMA	EYCVLIL.
OrthoDB	EOG4KWJTT.
PhylomeDB	Q13795.
Gene expression databases
ArrayExpress	Q13795.
Bgee	Q13795.
CleanEx	HS_ARFRP1.
Genevestigator	Q13795.
GermOnline	ENSG00000101246. Homo sapiens.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR. [Graphical view]
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00177. ARF. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ARFRP1. human.
GenomeRNAi	10139.
NextBio	38359.
SOURCE	Search...

Entry information

Entry name

ARFRP_HUMAN

Accession

Primary (citable) accession number: Q13795
Secondary accession number(s): B7ZKX7, E1P5J9, Q6IBQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.