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Protein

Phorbol-12-myristate-13-acetate-induced protein 1

Gene

PMAIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.By similarity5 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000141682-MONOMER.
ReactomeiR-HSA-111448. Activation of NOXA and translocation to mitochondria.
R-HSA-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-HSA-6803204. TP53 Regulates Transcription of Genes Involved in Cytochrome C Release.
SIGNORiQ13794.

Names & Taxonomyi

Protein namesi
Recommended name:
Phorbol-12-myristate-13-acetate-induced protein 1
Short name:
PMA-induced protein 1
Alternative name(s):
Immediate-early-response protein APR
Protein Noxa
Gene namesi
Name:PMAIP1
Synonyms:NOXA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:9108. PMAIP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • mitochondrial outer membrane Source: Reactome
  • mitochondrion Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29L → A: Reduced interaction with BAX. 2 Publications1
Mutagenesisi29L → E: Loss of interaction with MCL1 and of increased MCL1 degradation; when associated with E-32 and E-32. 2 Publications1
Mutagenesisi32F → E: Loss of interaction with MCL1 and of increased MCL1 degradation; when associated with E-29 and E-36. 2 Publications1
Mutagenesisi32F → I: Alters specificity of protein interaction and enhances pro-apoptotic activity; when associated with E-35. 2 Publications1
Mutagenesisi35K → E: Alters specificity of protein interaction and enhances pro-apoptotic activity; when associated with I-32. 1 Publication1
Mutagenesisi36L → E: Loss of interaction with MCL1 and of increased MCL1 degradation; when associated with E-29 and E-32. 1 Publication1

Organism-specific databases

DisGeNETi5366.
OpenTargetsiENSG00000141682.
PharmGKBiPA33434.

Polymorphism and mutation databases

BioMutaiPMAIP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000646441 – 54Phorbol-12-myristate-13-acetate-induced protein 1Add BLAST54

Proteomic databases

EPDiQ13794.
MaxQBiQ13794.
PaxDbiQ13794.
PeptideAtlasiQ13794.
PRIDEiQ13794.

PTM databases

iPTMnetiQ13794.
PhosphoSitePlusiQ13794.

Expressioni

Tissue specificityi

Highly expressed in adult T-cell leukemia cell line.

Inductioni

Up-regulated by p53/TP53, phorbol esters, double-stranded RNA, IFNB1/IFN-beta and viruses.3 Publications

Gene expression databases

BgeeiENSG00000141682.
CleanExiHS_PMAIP1.
ExpressionAtlasiQ13794. baseline and differential.
GenevisibleiQ13794. HS.

Organism-specific databases

HPAiHPA051063.

Interactioni

Subunit structurei

Interacts with MCL1, BCL2A1 and BAX.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2P104153EBI-707392,EBI-77694
BCL2P10415-13EBI-707392,EBI-4370304
MCL1Q078205EBI-707392,EBI-1003422

Protein-protein interaction databases

BioGridi111379. 11 interactors.
IntActiQ13794. 11 interactors.
MINTiMINT-1391244.
STRINGi9606.ENSP00000326119.

Structurei

Secondary structure

154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 40Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MQPX-ray2.24B19-43[»]
ProteinModelPortaliQ13794.
SMRiQ13794.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13794.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 50Required for mitochondrial location10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 37BH39

Domaini

The BH3 motif is essential for pro-apoptotic activity.

Sequence similaritiesi

Belongs to the PMAIP1 family.Curated

Phylogenomic databases

eggNOGiENOG410J6XT. Eukaryota.
ENOG4111AWA. LUCA.
GeneTreeiENSGT00530000065105.
HOGENOMiHOG000034020.
HOVERGENiHBG004273.
InParanoidiQ13794.
KOiK10131.
OMAiWRQTELP.
OrthoDBiEOG091G0V5H.
PhylomeDBiQ13794.

Family and domain databases

InterProiIPR024140. Noxa.
[Graphical view]
PANTHERiPTHR14299. PTHR14299. 1 hit.
PfamiPF15150. PMAIP1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13794-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGKKARKNA QPSPARAPAE LEVECATQLR RFGDKLNFRQ KLLNLISKLF

CSGT
Length:54
Mass (Da):6,030
Last modified:November 1, 1996 - v1
Checksum:i291A142B27167E70
GO
Isoform 2 (identifier: Q13794-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-54: ELEVECATQLRRFGDKLNFRQKLLNLISKLFCSGT → GPAGTAGTAR...HQFEERLHCN

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):14,955
Checksum:i055764B25628E4FC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05624720 – 54ELEVE…FCSGT → GPAGTAGTARDQAGFAIGMQ LRFTRGKKLLSSSLSSSPLA LPRGHEEQVQVAGSRVCYST QEIWRQTELPAETSESDIQT LLLRNLTASKTCMRGLLQKS FLRRCTFHQFEERLHCN in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90070 mRNA. Translation: BAA14111.1.
AK311943 mRNA. Translation: BAG34884.1.
AC107990 Genomic DNA. No translation available.
BC013120 mRNA. Translation: AAH13120.1.
BC032663 mRNA. Translation: AAH32663.1.
CCDSiCCDS11975.1. [Q13794-1]
PIRiI37018.
RefSeqiNP_066950.1. NM_021127.2. [Q13794-1]
UniGeneiHs.96.

Genome annotation databases

EnsembliENST00000269518; ENSP00000269518; ENSG00000141682. [Q13794-2]
ENST00000316660; ENSP00000326119; ENSG00000141682. [Q13794-1]
GeneIDi5366.
KEGGihsa:5366.
UCSCiuc002lic.3. human. [Q13794-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90070 mRNA. Translation: BAA14111.1.
AK311943 mRNA. Translation: BAG34884.1.
AC107990 Genomic DNA. No translation available.
BC013120 mRNA. Translation: AAH13120.1.
BC032663 mRNA. Translation: AAH32663.1.
CCDSiCCDS11975.1. [Q13794-1]
PIRiI37018.
RefSeqiNP_066950.1. NM_021127.2. [Q13794-1]
UniGeneiHs.96.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MQPX-ray2.24B19-43[»]
ProteinModelPortaliQ13794.
SMRiQ13794.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111379. 11 interactors.
IntActiQ13794. 11 interactors.
MINTiMINT-1391244.
STRINGi9606.ENSP00000326119.

PTM databases

iPTMnetiQ13794.
PhosphoSitePlusiQ13794.

Polymorphism and mutation databases

BioMutaiPMAIP1.

Proteomic databases

EPDiQ13794.
MaxQBiQ13794.
PaxDbiQ13794.
PeptideAtlasiQ13794.
PRIDEiQ13794.

Protocols and materials databases

DNASUi5366.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269518; ENSP00000269518; ENSG00000141682. [Q13794-2]
ENST00000316660; ENSP00000326119; ENSG00000141682. [Q13794-1]
GeneIDi5366.
KEGGihsa:5366.
UCSCiuc002lic.3. human. [Q13794-1]

Organism-specific databases

CTDi5366.
DisGeNETi5366.
GeneCardsiPMAIP1.
HGNCiHGNC:9108. PMAIP1.
HPAiHPA051063.
MIMi604959. gene.
neXtProtiNX_Q13794.
OpenTargetsiENSG00000141682.
PharmGKBiPA33434.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J6XT. Eukaryota.
ENOG4111AWA. LUCA.
GeneTreeiENSGT00530000065105.
HOGENOMiHOG000034020.
HOVERGENiHBG004273.
InParanoidiQ13794.
KOiK10131.
OMAiWRQTELP.
OrthoDBiEOG091G0V5H.
PhylomeDBiQ13794.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000141682-MONOMER.
ReactomeiR-HSA-111448. Activation of NOXA and translocation to mitochondria.
R-HSA-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.
R-HSA-6803204. TP53 Regulates Transcription of Genes Involved in Cytochrome C Release.
SIGNORiQ13794.

Miscellaneous databases

EvolutionaryTraceiQ13794.
GeneWikiiPhorbol-12-myristate-13-acetate-induced_protein_1.
GenomeRNAii5366.
PROiQ13794.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141682.
CleanExiHS_PMAIP1.
ExpressionAtlasiQ13794. baseline and differential.
GenevisibleiQ13794. HS.

Family and domain databases

InterProiIPR024140. Noxa.
[Graphical view]
PANTHERiPTHR14299. PTHR14299. 1 hit.
PfamiPF15150. PMAIP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPR_HUMAN
AccessioniPrimary (citable) accession number: Q13794
Secondary accession number(s): B2R4T7, Q8N589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.