Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Apolipoprotein F

Gene

APOF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.1 Publication

GO - Molecular functioni

  1. cholesterol binding Source: ProtInc
  2. lipid transporter activity Source: ProtInc
  3. receptor binding Source: ProtInc

GO - Biological processi

  1. cholesterol efflux Source: Ensembl
  2. cholesterol metabolic process Source: ProtInc
  3. lipid metabolic process Source: ProtInc
  4. lipid transport Source: ProtInc
  5. triglyceride metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Enzyme and pathway databases

ReactomeiREACT_267716. Orphan transporters.
REACT_6934. LDL-mediated lipid transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein F
Short name:
Apo-F
Alternative name(s):
Lipid transfer inhibitor protein
Short name:
LTIP
Gene namesi
Name:APOF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:615. APOF.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. high-density lipoprotein particle Source: UniProtKB-KW
  3. low-density lipoprotein particle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

HDL, LDL, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24902.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Propeptidei36 – 1641292 PublicationsPRO_0000002051Add
BLAST
Chaini165 – 326162Apolipoprotein FPRO_0000002052Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi274 – 2741O-linked (GalNAc...)1 Publication

Post-translational modificationi

O-glycosylated with core 1 or possibly core 8 glycans.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ13790.
PRIDEiQ13790.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.1 Publication

Gene expression databases

BgeeiQ13790.
CleanExiHS_APOF.
ExpressionAtlasiQ13790. baseline and differential.
GenevestigatoriQ13790.

Interactioni

Protein-protein interaction databases

BioGridi106816. 3 interactions.
STRINGi9606.ENSP00000381250.

Structurei

3D structure databases

ProteinModelPortaliQ13790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68175.
GeneTreeiENSGT00500000045104.
HOGENOMiHOG000034007.
HOVERGENiHBG050551.
InParanoidiQ13790.
OMAiPKSLPGF.
PhylomeDBiQ13790.
TreeFamiTF338778.

Family and domain databases

InterProiIPR026114. APOF.
[Graphical view]
PANTHERiPTHR15011. PTHR15011. 1 hit.
PfamiPF15148. Apolipo_F. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q13790-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGLCGYSAP DMRGLRLIMI PVELLLCYLL LHPVDATSYG KQTNVLMHFP
60 70 80 90 100
LSLESQTPSS DPLSCQFLHP KSLPGFSHMA PLPKFLVSLA LRNALEEAGC
110 120 130 140 150
QADVWALQLQ LYRQGGVNAT QVLIQHLRGL QKGRSTERNV SVEALASALQ
160 170 180 190 200
LLAREQQSTG RVGRSLPTED CENEKEQAVH NVVQLLPGVG TFYNLGTALY
210 220 230 240 250
YATQNCLGKA RERGRDGAID LGYDLLMTMA GMSGGPMGLA ISAALKPALR
260 270 280 290 300
SGVQQLIQYY QDQKDANISQ PETTKEGLRA ISDVSDLEET TTLASFISEV
310 320
VSSAPYWGWA IIKSYDLDPG AGSLEI
Length:326
Mass (Da):35,399
Last modified:April 5, 2011 - v2
Checksum:i046A1E775E7D4320
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti178 – 1781A → G.
Corresponds to variant rs11575216 [ dbSNP | Ensembl ].
VAR_055520

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27050 mRNA. Translation: AAA65642.1.
AC025574 Genomic DNA. No translation available.
BC026257 mRNA. Translation: AAH26257.1.
CCDSiCCDS44923.1.
PIRiJC2549.
RefSeqiNP_001629.1. NM_001638.2.
UniGeneiHs.534302.

Genome annotation databases

EnsembliENST00000398189; ENSP00000381250; ENSG00000175336.
GeneIDi319.
KEGGihsa:319.
UCSCiuc001sle.1. human.

Polymorphism databases

DMDMi327478500.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27050 mRNA. Translation: AAA65642.1.
AC025574 Genomic DNA. No translation available.
BC026257 mRNA. Translation: AAH26257.1.
CCDSiCCDS44923.1.
PIRiJC2549.
RefSeqiNP_001629.1. NM_001638.2.
UniGeneiHs.534302.

3D structure databases

ProteinModelPortaliQ13790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106816. 3 interactions.
STRINGi9606.ENSP00000381250.

Polymorphism databases

DMDMi327478500.

Proteomic databases

PaxDbiQ13790.
PRIDEiQ13790.

Protocols and materials databases

DNASUi319.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398189; ENSP00000381250; ENSG00000175336.
GeneIDi319.
KEGGihsa:319.
UCSCiuc001sle.1. human.

Organism-specific databases

CTDi319.
GeneCardsiGC12M056754.
HGNCiHGNC:615. APOF.
MIMi107760. gene.
neXtProtiNX_Q13790.
PharmGKBiPA24902.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG68175.
GeneTreeiENSGT00500000045104.
HOGENOMiHOG000034007.
HOVERGENiHBG050551.
InParanoidiQ13790.
OMAiPKSLPGF.
PhylomeDBiQ13790.
TreeFamiTF338778.

Enzyme and pathway databases

ReactomeiREACT_267716. Orphan transporters.
REACT_6934. LDL-mediated lipid transport.

Miscellaneous databases

GeneWikiiAPOF.
GenomeRNAii319.
NextBioi1307.
PROiQ13790.
SOURCEiSearch...

Gene expression databases

BgeeiQ13790.
CleanExiHS_APOF.
ExpressionAtlasiQ13790. baseline and differential.
GenevestigatoriQ13790.

Family and domain databases

InterProiIPR026114. APOF.
[Graphical view]
PANTHERiPTHR15011. PTHR15011. 1 hit.
PfamiPF15148. Apolipo_F. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 165-194 AND 251-265, TISSUE SPECIFICITY.
    Tissue: Hepatoma.
  2. "Molecular cloning and expression of lipid transfer inhibitor protein reveals its identity with apolipoprotein F."
    Wang X., Driscoll D.M., Morton R.E.
    J. Biol. Chem. 274:1814-1820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 165-178, FUNCTION.
    Tissue: Liver and Plasma.
  3. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Isolation and partial characterization of a new acidic apolipoprotein (apolipoprotein F) from high density lipoproteins of human plasma."
    Olofsson S.-O., McConathy W.J., Alaupovic P.
    Biochemistry 17:1032-1036(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
    Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G.
    Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT THR-274, STRUCTURE OF CARBOHYDRATES, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiAPOF_HUMAN
AccessioniPrimary (citable) accession number: Q13790
Secondary accession number(s): Q8TC13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 5, 2011
Last modified: January 7, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-12 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.