Q13790 (APOF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apolipoprotein F
Short name=Apo-F

Alternative name(s):
Lipid transfer inhibitor protein
Short name=LTIP

Gene names

Name:

APOF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the liver and secreted in plasma. Ref.1
Post-translational modification	O-glycosylated with core 1 or possibly core 8 glycans. Ref.6
Caution	It is uncertain whether Met-1 or Met-12 is the initiator.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Lipid transport Steroid metabolism Sterol metabolism Transport
Cellular component	HDL LDL Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol efflux Inferred from electronic annotation. Source: Compara cholesterol metabolic process Traceable author statement PubMed 6816269. Source: ProtInc triglyceride metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Non-traceable author statement PubMed 14718574. Source: UniProtKB high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cholesterol binding Traceable author statement PubMed 6816269. Source: ProtInc lipid transporter activity Traceable author statement PubMed 6816269. Source: ProtInc receptor binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Propeptide	36 – 164	129			PRO_0000002051
	Chain	165 – 326	162	Apolipoprotein F		PRO_0000002052
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	O-linked (GalNAc...) Ref.6
Natural variations
	Natural variant	178	1	A → G. Corresponds to variant rs11575216 [ dbSNP | Ensembl ].		VAR_055520

Sequences

	Sequence		Length	Mass (Da)
	Q13790 [UniParc]. Last modified April 5, 2011. Version 2. Checksum: 046A1E775E7D4320	FASTA	326	35,399
	10 20 30 40 50 60 MTGLCGYSAP DMRGLRLIMI PVELLLCYLL LHPVDATSYG KQTNVLMHFP LSLESQTPSS 70 80 90 100 110 120 DPLSCQFLHP KSLPGFSHMA PLPKFLVSLA LRNALEEAGC QADVWALQLQ LYRQGGVNAT 130 140 150 160 170 180 QVLIQHLRGL QKGRSTERNV SVEALASALQ LLAREQQSTG RVGRSLPTED CENEKEQAVH 190 200 210 220 230 240 NVVQLLPGVG TFYNLGTALY YATQNCLGKA RERGRDGAID LGYDLLMTMA GMSGGPMGLA 250 260 270 280 290 300 ISAALKPALR SGVQQLIQYY QDQKDANISQ PETTKEGLRA ISDVSDLEET TTLASFISEV 310 320 VSSAPYWGWA IIKSYDLDPG AGSLEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and molecular cloning of human apolipoprotein F." Day J.R., Albers J.J., Gilbert T.L., Whitmore T.E., McConathy W.J., Wolfbauer G. Biochem. Biophys. Res. Commun. 203:1146-1151(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 165-194 AND 251-265, TISSUE SPECIFICITY. Tissue: Hepatoma.
[2]	"Molecular cloning and expression of lipid transfer inhibitor protein reveals its identity with apolipoprotein F." Wang X., Driscoll D.M., Morton R.E. J. Biol. Chem. 274:1814-1820(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 165-178, FUNCTION. Tissue: Liver and Plasma.
[3]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[5]	"Isolation and partial characterization of a new acidic apolipoprotein (apolipoprotein F) from high density lipoproteins of human plasma." Olofsson S.-O., McConathy W.J., Alaupovic P. Biochemistry 17:1032-1036(1978) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD." Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G. Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT THR-274, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27050 mRNA. Translation: AAA65642.1. AC025574 Genomic DNA. No translation available. BC026257 mRNA. Translation: AAH26257.1.
IPI	IPI00299435.
PIR	JC2549.
RefSeq	NP_001629.1. NM_001638.2.
UniGene	Hs.534302.
3D structure databases
ProteinModelPortal	Q13790.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000381250.
Polymorphism databases
DMDM	2492916.
Proteomic databases
PaxDb	Q13790.
PRIDE	Q13790.
Protocols and materials databases
DNASU	319.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000398189; ENSP00000381250; ENSG00000175336.
GeneID	319.
KEGG	hsa:319.
UCSC	uc001sle.1. human.
Organism-specific databases
CTD	319.
GeneCards	GC12M056754.
HGNC	HGNC:615. APOF.
MIM	107760. gene.
neXtProt	NX_Q13790.
PharmGKB	PA24902.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG68175.
HOGENOM	HOG000034007.
HOVERGEN	HBG050551.
InParanoid	Q13790.
OMA	PKSLPGF.
OrthoDB	EOG49CQ80.
Gene expression databases
ArrayExpress	Q13790.
Bgee	Q13790.
CleanEx	HS_APOF.
Genevestigator	Q13790.
GermOnline	ENSG00000175336. Homo sapiens.
Family and domain databases
InterPro	IPR026114. APOF. [Graphical view]
PANTHER	PTHR15011. PTHR15011. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	319.
NextBio	1307.
SOURCE	Search...

Entry information

Entry name

APOF_HUMAN

Accession

Primary (citable) accession number: Q13790
Secondary accession number(s): Q8TC13

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 5, 2011
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.