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Q13765 (NACA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nascent polypeptide-associated complex subunit alpha

Short name=NAC-alpha
Alternative name(s):
Alpha-NAC
Allergen=Hom s 2
Gene names
Name:NACA
ORF Names:HSD48
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. Ref.12 Ref.13 Ref.16

Subunit structure

Interacts with TBP and JUN By similarity. Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains. Interacts with ASFV protein H339R. Ref.13 Ref.14

Subcellular location

Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Also found in nucleus. Ref.13 Ref.15

Tissue specificity

Ubiquitously expressed. Ref.17

Post-translational modification

Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24

Allergenic properties

Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations. Ref.11

Sequence similarities

Belongs to the NAC-alpha family.

Contains 1 NAC-A/B (NAC-alpha/beta) domain.

Contains 1 UBA domain.

Sequence caution

The sequence AAV83778.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 215215Nascent polypeptide-associated complex subunit alpha
PRO_0000135576

Regions

Domain70 – 13566NAC-A/B
Domain176 – 21338UBA
Region69 – 8012Required for DNA-binding By similarity

Amino acid modifications

Modified residue431Phosphoserine; by ILK1 By similarity
Modified residue921Phosphothreonine Ref.18
Modified residue1081N6-acetyllysine Ref.25
Modified residue1421N6-acetyllysine Ref.25
Modified residue1591Phosphothreonine; by GSK3-beta By similarity
Modified residue1611Phosphothreonine Ref.22 Ref.23
Modified residue1661Phosphoserine Ref.10 Ref.19 Ref.21 Ref.22 Ref.23 Ref.24
Modified residue1861Phosphoserine Ref.20
Modified residue1911Phosphoserine Ref.22

Experimental info

Sequence conflict2131L → S in BAD96805. Ref.6

Secondary structure

......... 215
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q13765 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 05DC563A8BEF307C

FASTA21523,384
        10         20         30         40         50         60 
MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID 

        70         80         90        100        110        120 
EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY 

       130        140        150        160        170        180 
IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD 

       190        200        210 
IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM 

« Hide

References

« Hide 'large scale' references
[1]"Novel products of the HuD, HuC, NNP-1 and alpha-internexin genes identified by autologous antibody screening of a pediatric neuroblastoma library."
Behrends U., Jandl T., Golbeck A., Lechner B., Mueller-Weihrich S., Schmid I., Till H., Berthold F., Voltz R., Mautner J.M.
Int. J. Cancer 100:669-677(2002) [PubMed: 12209604] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Neuroblastoma.
[2]"Investigation of alpha nascent polypeptide-associated complex functions in a human CD8(+) T cell ex vivo expansion model using antisense oligonucleotides."
Al-Shanti N., Steward C.G., Garland R.J., Rowbottom A.W.
Immunology 112:397-403(2004) [PubMed: 15196207] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Nascent polypeptide-associate complex (NAC): a novel type of polypeptide binding protein."
Sakai H., Chew C., Wang S., Wiedmann B., Geromanos S., Tempst P., Wiedmann M.
Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Umbilical cord blood.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Cervix.
[8]"A new spermatogenesis-related gene."
Yuan L.G., Tian Y.Q., Qiao Y., Miao S.Y., Wang L.F.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-173.
Tissue: Testis.
[9]Lubec G., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 101-142 AND 180-192, MASS SPECTROMETRY.
Tissue: Fetal brain cortex.
[10]Bienvenut W.V., Waridel P., Quadroni M.
Submitted (MAR-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 101-142; 145-192 AND 201-215, PHOSPHORYLATION AT SER-166, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[11]"Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients."
Natter S., Seiberler S., Hufnagl P., Binder B.R., Hirschl A.M., Ring J., Abeck D., Schmidt T., Valent P., Valenta R.
FASEB J. 12:1559-1569(1998) [PubMed: 9806765] [Abstract]
Cited for: ALLERGEN.
[12]"Unregulated exposure of the ribosomal M-site caused by NAC depletion results in delivery of non-secretory polypeptides to the Sec61 complex."
Moeller I., Beatrix B., Kreibich G., Sakai H., Lauring B., Wiedmann M.
FEBS Lett. 441:1-5(1998) [PubMed: 9877153] [Abstract]
Cited for: FUNCTION.
[13]"The alpha and beta subunit of the nascent polypeptide-associated complex have distinct functions."
Beatrix B., Sakai H., Wiedmann M.
J. Biol. Chem. 275:37838-37845(2000) [PubMed: 10982809] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BTF3, ASSOCIATION WITH RIBOSOMES, SUBCELLULAR LOCATION.
[14]"The alpha-chain of the nascent polypeptide-associated complex binds to and regulates FADD function."
Stilo R., Liguoro D., di Jeso B., Leonardi A., Vito P.
Biochem. Biophys. Res. Commun. 303:1034-1041(2003) [PubMed: 12684039] [Abstract]
Cited for: INTERACTION WITH FADD.
[15]"Activation of the JNK-AP-1 signal transduction pathway is associated with pathogenesis and progression of human osteosarcomas."
Papachristou D.J., Batistatou A., Sykiotis G.P., Varakis I., Papavassiliou A.G.
Bone 32:364-371(2003) [PubMed: 12689679] [Abstract]
Cited for: SUBCELLULAR LOCATION, POSSIBLE INVOLVEMENT IN OSTEOSARCOMA.
[16]"NACA is a positive regulator of human erythroid-cell differentiation."
Lopez S., Stuhl L., Fichelson S., Dubart-Kupperschmitt A., St Arnaud R., Galindo J.-R., Murati A., Berda N., Dubreuil P., Gomez S.
J. Cell Sci. 118:1595-1605(2005) [PubMed: 15784678] [Abstract]
Cited for: FUNCTION.
[17]"Emergence of young human genes after a burst of retroposition in primates."
Marques A.C., Dupanloup I., Vinckenbosch N., Reymond A., Kaessmann H.
PLoS Biol. 3:E357-E357(2005) [PubMed: 16201836] [Abstract]
Cited for: TISSUE SPECIFICITY.
[18]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-92, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[19]"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry."
Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.
Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
[20]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
[21]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[22]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161; SER-166 AND SER-191, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[23]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161 AND SER-166, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
[24]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, MASS SPECTROMETRY.
Tissue: Leukemic T-cell.
[25]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed: 19608861] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-108 AND LYS-142, MASS SPECTROMETRY.
[26]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY034001 mRNA. Translation: AAK57544.1.
AY911673 mRNA. Translation: AAX14393.1.
X80909 mRNA. Translation: CAA56869.1.
AF054187 mRNA. Translation: AAC99403.1.
CR450295 mRNA. Translation: CAG29291.1.
AK223085 mRNA. Translation: BAD96805.1.
BC105120 mRNA. Translation: AAI05121.1.
BC105122 mRNA. Translation: AAI05123.1.
BC106041 mRNA. Translation: AAI06042.1.
AY605660 mRNA. Translation: AAV83778.1. Different initiation.
IPIIPI00023748.
PIRS49326.
RefSeqNP_001106673.1. NM_001113202.1.
NP_001106674.2. NM_001113203.2.
UniGeneHs.505735.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50B84-136[»]
3MCBX-ray1.90A79-132[»]
3MCEX-ray2.40A/B/C/D81-133[»]
ProteinModelPortalQ13765.
SMRQ13765. Positions 79-213.
ModBaseSearch...

Protein-protein interaction databases

IntActQ13765. 14 interactions.
MINTMINT-5000033.
STRINGQ13765.

Protein family/group databases

Allergome3323. Hom s 2.0101.
412. Hom s 2.

PTM databases

PhosphoSiteQ13765.

Polymorphism databases

DMDM71151996.

Proteomic databases

PRIDEQ13765.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356769; ENSP00000349212; ENSG00000196531.
ENST00000393891; ENSP00000377469; ENSG00000196531.
ENST00000435567; ENSP00000397352; ENSG00000196531.
GeneID4666.
KEGGhsa:4666.
UCSCuc001sly.2. human.

Organism-specific databases

CTD4666.
GeneCardsGC12M057078.
H-InvDBHIX0034277.
HIX0201818.
HGNCHGNC:7629. NACA.
MIM601234. gene.
neXtProtNX_Q13765.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG16779.
HOVERGENHBG082004.
OrthoDBEOG41RPW6.
PhylomeDBQ13765.

Gene expression databases

ArrayExpressQ13765.
BgeeQ13765.
CleanExHS_NACA.
GenevestigatorQ13765.
GermOnlineENSG00000196531. Homo sapiens.

Family and domain databases

InterProIPR002715. Nas_poly-pep-assoc_cplx.
IPR016641. Nas_poly-pep-assoc_cplx_asu.
[Graphical view]
KOK03626.
PfamPF01849. NAC. 1 hit.
[Graphical view]
PIRSFPIRSF015901. NAC_alpha. 1 hit.
PROSITEPS51151. NAC_AB. 1 hit.
PS50030. UBA. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio17982.
SOURCESearch...

Entry information

Entry nameNACA_HUMAN
AccessionPrimary (citable) accession number: Q13765
Secondary accession number(s): Q3KQV4, Q53A18, Q53G46
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: January 25, 2012
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Allergens

Nomenclature of allergens and list of entries

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families