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Q13740 (CD166_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD166 antigen
Alternative name(s):
Activated leukocyte cell adhesion molecule
CD_antigen=CD166
Gene names
Name:ALCAM
Synonyms:MEMD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

Subcellular location

Membrane; Single-pass type I membrane protein Ref.2.

Isoform 3: Secreted Ref.2.

Tissue specificity

Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. Ref.7

Domain

The CD6 binding site is located in the N-terminal Ig-like domain. Ref.8

Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13740-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13740-2)

The sequence of this isoform differs from the canonical sequence as follows:
     503-516: ISIPEHDEADEISD → N
Isoform 3 (identifier: Q13740-3)

Also known as: sALCAM;

The sequence of this isoform differs from the canonical sequence as follows:
     132-133: KQ → SK
     134-583: Missing.
Note: Secreted form, inhibits isoform 1 homophilic interaction.
Isoform 4 (identifier: Q13740-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-191: Missing.
     244-330: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 583556CD166 antigen
PRO_0000014659

Regions

Topological domain28 – 527500Extracellular Potential
Transmembrane528 – 54922Helical; Potential
Topological domain550 – 58334Cytoplasmic Potential
Domain28 – 12093Ig-like V-type 1
Domain125 – 234110Ig-like V-type 2
Domain245 – 32884Ig-like C2-type 1
Domain333 – 40977Ig-like C2-type 2
Domain416 – 50186Ig-like C2-type 3

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) Ref.9 Ref.11
Glycosylation951N-linked (GlcNAc...) Ref.10 Ref.11
Glycosylation1671N-linked (GlcNAc...) Ref.9
Glycosylation2651N-linked (GlcNAc...) Ref.10
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation4571N-linked (GlcNAc...) Ref.11 Ref.12
Glycosylation4661N-linked (GlcNAc...); atypical Ref.12
Glycosylation4801N-linked (GlcNAc...) Ref.9 Ref.11
Glycosylation4991N-linked (GlcNAc...) Ref.9 Ref.10
Disulfide bond43 ↔ 113 Potential
Disulfide bond157 ↔ 220 Potential
Disulfide bond270 ↔ 313 Potential
Disulfide bond354 ↔ 392 Potential
Disulfide bond435 ↔ 485 Potential

Natural variations

Alternative sequence1 – 191191Missing in isoform 4.
VSP_053529
Alternative sequence132 – 1332KQ → SK in isoform 3.
VSP_053530
Alternative sequence134 – 583450Missing in isoform 3.
VSP_053531
Alternative sequence244 – 33087Missing in isoform 4.
VSP_053532
Alternative sequence503 – 51614ISIPE…DEISD → N in isoform 2.
VSP_021797
Natural variant2291G → D.
Corresponds to variant rs10933819 [ dbSNP | Ensembl ].
VAR_029514
Natural variant2581N → S.
Corresponds to variant rs1044240 [ dbSNP | Ensembl ].
VAR_003907
Natural variant3011T → M. Ref.1
Corresponds to variant rs1044243 [ dbSNP | Ensembl ].
VAR_003908
Natural variant3151L → M.
Corresponds to variant rs12629872 [ dbSNP | Ensembl ].
VAR_029515
Natural variant3521V → M.
Corresponds to variant rs2291375 [ dbSNP | Ensembl ].
VAR_029516
Natural variant3671M → I.
Corresponds to variant rs34926152 [ dbSNP | Ensembl ].
VAR_049856

Secondary structure

................... 583
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: 68FEFD67C14B0C57

FASTA58365,102
        10         20         30         40         50         60 
MESKGASSCR LLFCLLISAT VFRPGLGWYT VNSAYGDTII IPCRLDVPQN LMFGKWKYEK 

        70         80         90        100        110        120 
PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL NLSENYTLSI SNARISDEKR FVCMLVTEDN 

       130        140        150        160        170        180 
VFEAPTIVKV FKQPSKPEIV SKALFLETEQ LKKLGDCISE DSYPDGNITW YRNGKVLHPL 

       190        200        210        220        230        240 
EGAVVIIFKK EMDPVTQLYT MTSTLEYKTT KADIQMPFTC SVTYYGPSGQ KTIHSEQAVF 

       250        260        270        280        290        300 
DIYYPTEQVT IQVLPPKNAI KEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL 

       310        320        330        340        350        360 
TDVRRNATGD YKCSLIDKKS MIASTAITVH YLDLSLNPSG EVTRQIGDAL PVSCTISASR 

       370        380        390        400        410        420 
NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM 

       430        440        450        460        470        480 
TKKTDPSGLS KTIICHVEGF PKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN 

       490        500        510        520        530        540 
VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADEISDENRE KVNDQAKLIV GIVVGLLLAA 

       550        560        570        580 
LVAGVVYWLY MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA 

« Hide

Isoform 2 [UniParc].

Checksum: 4B61C53E7C61CACC
Show »

FASTA57063,665
Isoform 3 (sALCAM) [UniParc].

Checksum: C7B35209EA8BFC35
Show »

FASTA13315,109
Isoform 4 [UniParc].

Checksum: FB5F5FBB6D4E6DCC
Show »

FASTA30533,878

References

« Hide 'large scale' references
[1]"Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand."
Bowen M.A., Patel D.D., Li X., Modrell B., Malacko A.R., Wang W.-C., Marquardt H., Neubauer M., Pesando J.M., Francke U., Haynes B.F., Aruffo A.
J. Exp. Med. 181:2213-2220(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT MET-301, PARTIAL PROTEIN SEQUENCE.
[2]"Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function."
Ikeda K., Quertermous T.
J. Biol. Chem. 279:55315-55323(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORM 3).
[3]"A novel alternatively spliced variant of activated leukocyte cell adhesion molecule (ALCAM/CD166) resulting from a deletion in the extracellular membrane proximal stem."
Abe Y., Bui-Thanh N.-A., Smith C.W., Ballantyne C.M., Burns A.R.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[4]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
Tissue: Lung and Placenta.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain and Lung.
[7]"MEMD, a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell adhesion molecule)."
Degen W.G., van Kempen L.C., Gijzen E.G., van Groningen J.J., van Kooyk Y., Bloemers H.P.J., Swart G.W.
Am. J. Pathol. 152:805-813(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-583 (ISOFORM 1), TISSUE SPECIFICITY.
[8]"Recognition of diverse proteins by members of the immunoglobulin superfamily: delineation of the receptor binding site in the human CD6 ligand ALCAM."
Skonier J.E., Bowen M.A., Emswiler J., Aruffo A., Bajorath J.
Biochemistry 35:12287-12291(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN CD6 BINDING.
[9]"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
Zhang H., Li X.-J., Martin D.B., Aebersold R.
Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-91; ASN-167; ASN-480 AND ASN-499.
[10]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-95; ASN-265 AND ASN-499.
Tissue: Plasma.
[11]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-91; ASN-95; ASN-457 AND ASN-480.
Tissue: Liver.
[12]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-457 AND ASN-466.
Tissue: Leukemic T-cell.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Molecular model of the N-terminal receptor-binding domain of the human CD6 ligand ALCAM."
Bajorath J., Bowen M.A., Aruffo A.
Protein Sci. 4:1644-1647(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 28-133.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L38608 mRNA. Translation: AAB59499.1.
AY644765 mRNA. Translation: AAV28819.1.
DQ486139 mRNA. Translation: ABF48405.1.
DQ486140 mRNA. Translation: ABF48406.1.
AK127617 mRNA. Translation: BAC87059.1.
AK300362 mRNA. Translation: BAG62102.1.
AK316113 mRNA. Translation: BAH14484.1.
AC023602 Genomic DNA. No translation available.
AC078806 Genomic DNA. No translation available.
AC133476 Genomic DNA. No translation available.
BC057809 mRNA. Translation: AAH57809.1.
BC137096 mRNA. Translation: AAI37097.1.
BC137097 mRNA. Translation: AAI37098.1.
Y10183 mRNA. Translation: CAA71256.1.
CCDSCCDS33810.1. [Q13740-1]
CCDS58841.1. [Q13740-2]
PIRI39428.
RefSeqNP_001230209.1. NM_001243280.1. [Q13740-2]
NP_001230212.1. NM_001243283.1. [Q13740-3]
NP_001618.2. NM_001627.3. [Q13740-1]
UniGeneHs.591293.
Hs.740441.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KJCmodel-A28-133[»]
ProteinModelPortalQ13740.
SMRQ13740. Positions 31-224, 376-495.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106716. 4 interactions.
IntActQ13740. 2 interactions.
STRING9606.ENSP00000305988.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteQ13740.

Polymorphism databases

DMDM118572629.

Proteomic databases

MaxQBQ13740.
PaxDbQ13740.
PRIDEQ13740.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306107; ENSP00000305988; ENSG00000170017. [Q13740-1]
ENST00000389927; ENSP00000374577; ENSG00000170017. [Q13740-4]
ENST00000472644; ENSP00000419236; ENSG00000170017. [Q13740-2]
GeneID214.
KEGGhsa:214.
UCSCuc003dvv.3. human.
uc003dvw.2. human. [Q13740-1]
uc003dvy.3. human.

Organism-specific databases

CTD214.
GeneCardsGC03P105085.
HGNCHGNC:400. ALCAM.
HPACAB002148.
HPA010926.
MIM601662. gene.
neXtProtNX_Q13740.
PharmGKBPA24691.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG148902.
HOGENOMHOG000070101.
HOVERGENHBG050847.
InParanoidQ13740.
KOK06547.
OMADIYYPTE.
OrthoDBEOG74J97B.
PhylomeDBQ13740.
TreeFamTF321859.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ13740.
BgeeQ13740.
CleanExHS_ALCAM.
GenevestigatorQ13740.

Family and domain databases

Gene3D2.60.40.10. 5 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 3 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSALCAM. human.
GeneWikiALCAM.
GenomeRNAi214.
NextBio866.
PMAP-CutDBQ13740.
PROQ13740.
SOURCESearch...

Entry information

Entry nameCD166_HUMAN
AccessionPrimary (citable) accession number: Q13740
Secondary accession number(s): B2RNS3 expand/collapse secondary AC list , B4DTU0, O60892, Q1HGM8, Q1HGM9, Q6PEY4, Q6ZS95
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries