Q136A2 (Y2859_RHOPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable RNA methyltransferase RPD_2859
EC=2.1.1.-

Gene names

Ordered Locus Names:

RPD_2859

Organism

Rhodopseudomonas palustris (strain BisB5) [Complete proteome] [HAMAP]

Taxonomic identifier

316057 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas › Rhodopseudomonas palustris

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the radical SAM superfamily. RlmN family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA methylation Inferred from electronic annotation. Source: GOC rRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Probable RNA methyltransferase RPD_2859		PRO_0000350372
Regions
	Region	162 – 163	2	S-adenosyl-L-methionine binding By similarity
	Region	217 – 219	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	99	1	Proton acceptor Potential
	Active site	336	1	S-methylcysteine intermediate By similarity
	Metal binding	119	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	123	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	126	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	194	1	S-adenosyl-L-methionine By similarity
	Binding site	293	1	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 336		(transient) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q136A2 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: CBEDC1DC5F019B71	FASTA	359	39,815
	10 20 30 40 50 60 MASAQRHSTP DLVRQRDPAG VSGLSLGLTS SEFAGLHEGA VPDVTYRELF FPHADPPRRL 70 80 90 100 110 120 QNWARSRGVR LRTLTRIGRD QGGRSEKMLF GLHDGYAVES VLIRRFDGHT ACISSQVGCA 130 140 150 160 170 180 FACRFCASGQ AGLMRNLEAG EIVEQVVRLG PKVNRIVFMG IGEPLNNYQQ VLKAIRILRD 190 200 210 220 230 240 RQGMNFPTTG ITLSTIGIPK ALKQLREEHL AINLTISLHA TTQEVRDRLI PGARKHPLGE 250 260 270 280 290 300 VVERACAWAR RHNRPVTFAY LVLPGINDSI ADARRLAAML RDSPARVNLM RWNPVDGVGL 310 320 330 340 350 QRTPDRSLAH FRTTLENALV PVVVRDTQGR DISAACGQLW LRDLKGLPVG NRPRQGRMT

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisB5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Lykidis A., Oda Y., Harwood C.S., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisB5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000283 Genomic DNA. Translation: ABE40087.1.
RefSeq	YP_569988.1. NC_007958.1.
3D structure databases
ProteinModelPortal	Q136A2.
ModBase	Search...
Protein-protein interaction databases
STRING	316057.RPD_2859.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE40087; ABE40087; RPD_2859.
GeneID	4023357.
KEGG	rpd:RPD_2859.
PATRIC	23280771. VBIRhoPal120395_2951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0820.
HOGENOM	HOG000217991.
KO	K06941.
OMA	PKMISKL.
Enzyme and pathway databases
BioCyc	RPAL316057:GHDC-2933-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR004383. rRNA_lsu_MTrfase_RlmN. IPR007197. rSAM. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF006004. CHP00048. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00048. TIGR00048. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Y2859_RHOPS

Accession

Primary (citable) accession number: Q136A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program