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Protein

Ras and Rab interactor 1

Gene

RIN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.3 Publications

GO - Molecular functioni

GO - Biological processi

  • associative learning Source: Ensembl
  • endocytosis Source: UniProtKB-KW
  • memory Source: Ensembl
  • negative regulation of synaptic plasticity Source: Ensembl
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionGTPase activation
Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-HSA-8876198 RAB GEFs exchange GTP for GDP on RABs
SignaLinkiQ13671
SIGNORiQ13671

Names & Taxonomyi

Protein namesi
Recommended name:
Ras and Rab interactor 1
Alternative name(s):
Ras inhibitor JC99
Ras interaction/interference protein 1
Gene namesi
Name:RIN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000174791.10
HGNCiHGNC:18749 RIN1
MIMi605965 gene
neXtProtiNX_Q13671

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi351S → A: Abolishes phosphorylation by PKD and the interaction with 14-3-3 proteins. 1 Publication1

Organism-specific databases

DisGeNETi9610
OpenTargetsiENSG00000174791
PharmGKBiPA38671

Polymorphism and mutation databases

BioMutaiRIN1
DMDMi116242760

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913171 – 783Ras and Rab interactor 1Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei36Phosphotyrosine; by ABL1 and ABL21 Publication1
Modified residuei210PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei337PhosphoserineCombined sources1
Modified residuei351Phosphoserine; by PKD/PRKD1Combined sources1 Publication1
Modified residuei609PhosphoserineCombined sources1
Modified residuei611PhosphoserineCombined sources1
Modified residuei692Omega-N-methylarginineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins.4 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ13671
MaxQBiQ13671
PaxDbiQ13671
PeptideAtlasiQ13671
PRIDEiQ13671
ProteomicsDBi59652
59653 [Q13671-2]

PTM databases

iPTMnetiQ13671
PhosphoSitePlusiQ13671

Expressioni

Tissue specificityi

Expressed in all tissues examined with high levels in brain, placenta and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000174791
CleanExiHS_RIN1
ExpressionAtlasiQ13671 baseline and differential
GenevisibleiQ13671 HS

Organism-specific databases

HPAiHPA035491

Interactioni

Subunit structurei

Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114972, 30 interactors
CORUMiQ13671
DIPiDIP-117N
IntActiQ13671, 50 interactors
MINTiQ13671
STRINGi9606.ENSP00000310406

Structurei

3D structure databases

ProteinModelPortaliQ13671
SMRiQ13671
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 163SH2PROSITE-ProRule annotationAdd BLAST95
Domaini456 – 598VPS9PROSITE-ProRule annotationAdd BLAST143
Domaini624 – 706Ras-associatingPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni294 – 727Ras and 14-3-3 protein binding regionAdd BLAST434

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi259 – 268Pro-rich10

Sequence similaritiesi

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG2320 Eukaryota
ENOG410ZZW5 LUCA
GeneTreeiENSGT00530000063053
HOGENOMiHOG000154128
HOVERGENiHBG036105
InParanoidiQ13671
KOiK17638
OMAiFFNPLFP
OrthoDBiEOG091G02V2
PhylomeDBiQ13671
TreeFamiTF331067

Family and domain databases

CDDicd10393 SH2_RIN1, 1 hit
Gene3Di1.20.1050.80, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000159 RA_dom
IPR035867 RIN1_SH2
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR003123 VPS9
IPR037191 VPS9_dom_sf
PfamiView protein in Pfam
PF00788 RA, 1 hit
PF02204 VPS9, 1 hit
SMARTiView protein in SMART
SM00314 RA, 1 hit
SM00252 SH2, 1 hit
SM00167 VPS9, 1 hit
SUPFAMiSSF109993 SSF109993, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50001 SH2, 1 hit
PS51205 VPS9, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RIN1 (identifier: Q13671-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESPGESGAG SPGAPSPSSF TTGHLAREKP AQDPLYDVPN ASGGQAGGPQ
60 70 80 90 100
RPGRVVSLRE RLLLTRPVWL QLQANAAAAL HMLRTEPPGT FLVRKSNTRQ
110 120 130 140 150
CQALCMRLPE ASGPSFVSSH YILESPGGVS LEGSELMFPD LVQLICAYCH
160 170 180 190 200
TRDILLLPLQ LPRAIHHAAT HKELEAISHL GIEFWSSSLN IKAQRGPAGG
210 220 230 240 250
PVLPQLKARS PQELDQGTGA ALCFFNPLFP GDLGPTKREK FKRSFKVRVS
260 270 280 290 300
TETSSPLSPP AVPPPPVPVL PGAVPSQTER LPPCQLLRRE SSVGYRVPAG
310 320 330 340 350
SGPSLPPMPS LQEVDCGSPS SSEEEGVPGS RGSPATSPHL GRRRPLLRSM
360 370 380 390 400
SAAFCSLLAP ERQVGRAAAA LMQDRHTAAG QLVQDLLTQV RAGPEPQELQ
410 420 430 440 450
GIRQALSRAR AMLSAELGPE KLLSPKRLEH VLEKSLHCSV LKPLRPILAA
460 470 480 490 500
RLRRRLAADG SLGRLAEGLR LARAQGPGAF GSHLSLPSPV ELEQVRQKLL
510 520 530 540 550
QLLRTYSPSA QVKRLLQACK LLYMALRTQE GEGAGADEFL PLLSLVLAHC
560 570 580 590 600
DLPELLLEAE YMSELLEPSL LTGEGGYYLT SLSASLALLS GLGQAHTLPL
610 620 630 640 650
SPVQELRRSL SLWEQRRLPA THCFQHLLRV AYQDPSSGCT SKTLAVPPEA
660 670 680 690 700
SIATLNQLCA TKFRVTQPNT FGLFLYKEQG YHRLPPGALA HRLPTTGYLV
710 720 730 740 750
YRRAEWPETQ GAVTEEEGSG QSEARSRGEE QGCQGDGDAG VKASPRDIRE
760 770 780
QSETTAEGGQ GQAQEGPAQP GEPEAEGSRA AEE
Length:783
Mass (Da):84,099
Last modified:October 17, 2006 - v4
Checksum:i2B4DA70147CDDDFE
GO
Isoform RIN1-delta (identifier: Q13671-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-490: Missing.

Note: Shows reduced ability to bind to Ras and 14-3-3 proteins.
Show »
Length:721
Mass (Da):77,500
Checksum:iAF59AA2FCA374ABE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti392 – 395AGPE → DGQR (PubMed:1849280).Curated4
Sequence conflicti392 – 395AGPE → DGQR (PubMed:7862125).Curated4
Sequence conflicti392 – 395AGPE → DGQR (PubMed:9144171).Curated4
Sequence conflicti400Q → E (PubMed:1849280).Curated1
Sequence conflicti400Q → E (PubMed:7862125).Curated1
Sequence conflicti400Q → E (PubMed:9144171).Curated1
Sequence conflicti423L → V (PubMed:1849280).Curated1
Sequence conflicti423L → V (PubMed:7862125).Curated1
Sequence conflicti423L → V (PubMed:9144171).Curated1
Sequence conflicti503L → V (PubMed:1849280).Curated1
Sequence conflicti503L → V (PubMed:7862125).Curated1
Sequence conflicti503L → V (PubMed:9144171).Curated1
Sequence conflicti534A → S (PubMed:1849280).Curated1
Sequence conflicti534A → S (PubMed:7862125).Curated1
Sequence conflicti534A → S (PubMed:9144171).Curated1
Sequence conflicti538E → G (PubMed:1849280).Curated1
Sequence conflicti538E → G (PubMed:7862125).Curated1
Sequence conflicti538E → G (PubMed:9144171).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004377429 – 490Missing in isoform RIN1-delta. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36463 mRNA Translation: AAB67270.1
BC014417 mRNA Translation: AAH14417.1
CCDSiCCDS31614.1 [Q13671-1]
PIRiA58613 A38637
RefSeqiNP_004283.2, NM_004292.2 [Q13671-1]
UniGeneiHs.1030

Genome annotation databases

EnsembliENST00000311320; ENSP00000310406; ENSG00000174791 [Q13671-1]
GeneIDi9610
KEGGihsa:9610
UCSCiuc001ohn.2 human [Q13671-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRIN1_HUMAN
AccessioniPrimary (citable) accession number: Q13671
Secondary accession number(s): O15010, Q00427, Q96CC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 17, 2006
Last modified: June 20, 2018
This is version 168 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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