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UniProtKB - Q13671 (RIN1_HUMAN)

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Protein

Ras and Rab interactor 1

Gene

RIN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.3 Publications

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionGTPase activation
Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.
SignaLinkiQ13671.
SIGNORiQ13671.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras and Rab interactor 1
Alternative name(s):
Ras inhibitor JC99
Ras interaction/interference protein 1
Gene namesi
Name:RIN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:18749. RIN1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • dendrite Source: Ensembl
  • neuronal cell body Source: Ensembl
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi351S → A: Abolishes phosphorylation by PKD and the interaction with 14-3-3 proteins. 1 Publication1

Organism-specific databases

DisGeNETi9610.
OpenTargetsiENSG00000174791.
PharmGKBiPA38671.

Polymorphism and mutation databases

BioMutaiRIN1.
DMDMi116242760.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913171 – 783Ras and Rab interactor 1Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei36Phosphotyrosine; by ABL1 and ABL21 Publication1
Modified residuei210PhosphoserineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei337PhosphoserineCombined sources1
Modified residuei351Phosphoserine; by PKD/PRKD1Combined sources1 Publication1
Modified residuei609PhosphoserineCombined sources1
Modified residuei611PhosphoserineCombined sources1
Modified residuei692Omega-N-methylarginineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins.4 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ13671.
MaxQBiQ13671.
PaxDbiQ13671.
PeptideAtlasiQ13671.
PRIDEiQ13671.

PTM databases

iPTMnetiQ13671.
PhosphoSitePlusiQ13671.

Expressioni

Tissue specificityi

Expressed in all tissues examined with high levels in brain, placenta and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000174791.
CleanExiHS_RIN1.
ExpressionAtlasiQ13671. baseline and differential.
GenevisibleiQ13671. HS.

Organism-specific databases

HPAiHPA035491.

Interactioni

Subunit structurei

Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

BioGridi114972. 27 interactors.
DIPiDIP-117N.
IntActiQ13671. 50 interactors.
MINTiMINT-1347026.
STRINGi9606.ENSP00000310406.

Structurei

3D structure databases

ProteinModelPortaliQ13671.
SMRiQ13671.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 163SH2PROSITE-ProRule annotationAdd BLAST95
Domaini456 – 598VPS9PROSITE-ProRule annotationAdd BLAST143
Domaini624 – 706Ras-associatingPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni294 – 727Ras and 14-3-3 protein binding regionAdd BLAST434

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi259 – 268Pro-rich10

Sequence similaritiesi

Belongs to the RIN (Ras interaction/interference) family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG2320. Eukaryota.
ENOG410ZZW5. LUCA.
GeneTreeiENSGT00530000063053.
HOGENOMiHOG000154128.
HOVERGENiHBG036105.
InParanoidiQ13671.
KOiK17638.
OMAiFFNPLFP.
OrthoDBiEOG091G02V2.
PhylomeDBiQ13671.
TreeFamiTF331067.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR000159. RA_dom.
IPR000980. SH2.
IPR003123. VPS9.
PfamiView protein in Pfam
PF00788. RA. 1 hit.
PF02204. VPS9. 1 hit.
SMARTiView protein in SMART
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
PS51205. VPS9. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RIN1 (identifier: Q13671-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESPGESGAG SPGAPSPSSF TTGHLAREKP AQDPLYDVPN ASGGQAGGPQ 
        60         70         80         90        100
RPGRVVSLRE RLLLTRPVWL QLQANAAAAL HMLRTEPPGT FLVRKSNTRQ 
       110        120        130        140        150
CQALCMRLPE ASGPSFVSSH YILESPGGVS LEGSELMFPD LVQLICAYCH 
       160        170        180        190        200
TRDILLLPLQ LPRAIHHAAT HKELEAISHL GIEFWSSSLN IKAQRGPAGG 
       210        220        230        240        250
PVLPQLKARS PQELDQGTGA ALCFFNPLFP GDLGPTKREK FKRSFKVRVS 
       260        270        280        290        300
TETSSPLSPP AVPPPPVPVL PGAVPSQTER LPPCQLLRRE SSVGYRVPAG 
       310        320        330        340        350
SGPSLPPMPS LQEVDCGSPS SSEEEGVPGS RGSPATSPHL GRRRPLLRSM 
       360        370        380        390        400
SAAFCSLLAP ERQVGRAAAA LMQDRHTAAG QLVQDLLTQV RAGPEPQELQ 
       410        420        430        440        450
GIRQALSRAR AMLSAELGPE KLLSPKRLEH VLEKSLHCSV LKPLRPILAA 
       460        470        480        490        500
RLRRRLAADG SLGRLAEGLR LARAQGPGAF GSHLSLPSPV ELEQVRQKLL 
       510        520        530        540        550
QLLRTYSPSA QVKRLLQACK LLYMALRTQE GEGAGADEFL PLLSLVLAHC 
       560        570        580        590        600
DLPELLLEAE YMSELLEPSL LTGEGGYYLT SLSASLALLS GLGQAHTLPL 
       610        620        630        640        650
SPVQELRRSL SLWEQRRLPA THCFQHLLRV AYQDPSSGCT SKTLAVPPEA 
       660        670        680        690        700
SIATLNQLCA TKFRVTQPNT FGLFLYKEQG YHRLPPGALA HRLPTTGYLV 
       710        720        730        740        750
YRRAEWPETQ GAVTEEEGSG QSEARSRGEE QGCQGDGDAG VKASPRDIRE 
       760        770        780 
QSETTAEGGQ GQAQEGPAQP GEPEAEGSRA AEE
Length:783
Mass (Da):84,099
Last modified:October 17, 2006 - v4
Checksum:i2B4DA70147CDDDFE
GO
Isoform RIN1-delta (identifier: Q13671-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-490: Missing.

Note: Shows reduced ability to bind to Ras and 14-3-3 proteins.
Show »
Length:721
Mass (Da):77,500
Checksum:iAF59AA2FCA374ABE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti392 – 395AGPE → DGQR (PubMed:1849280).Curated4
Sequence conflicti392 – 395AGPE → DGQR (PubMed:7862125).Curated4
Sequence conflicti392 – 395AGPE → DGQR (PubMed:9144171).Curated4
Sequence conflicti400Q → E (PubMed:1849280).Curated1
Sequence conflicti400Q → E (PubMed:7862125).Curated1
Sequence conflicti400Q → E (PubMed:9144171).Curated1
Sequence conflicti423L → V (PubMed:1849280).Curated1
Sequence conflicti423L → V (PubMed:7862125).Curated1
Sequence conflicti423L → V (PubMed:9144171).Curated1
Sequence conflicti503L → V (PubMed:1849280).Curated1
Sequence conflicti503L → V (PubMed:7862125).Curated1
Sequence conflicti503L → V (PubMed:9144171).Curated1
Sequence conflicti534A → S (PubMed:1849280).Curated1
Sequence conflicti534A → S (PubMed:7862125).Curated1
Sequence conflicti534A → S (PubMed:9144171).Curated1
Sequence conflicti538E → G (PubMed:1849280).Curated1
Sequence conflicti538E → G (PubMed:7862125).Curated1
Sequence conflicti538E → G (PubMed:9144171).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004377429 – 490Missing in isoform RIN1-delta. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36463 mRNA. Translation: AAB67270.1.
BC014417 mRNA. Translation: AAH14417.1.
CCDSiCCDS31614.1. [Q13671-1]
PIRiA58613. A38637.
RefSeqiNP_004283.2. NM_004292.2. [Q13671-1]
UniGeneiHs.1030.

Genome annotation databases

EnsembliENST00000311320; ENSP00000310406; ENSG00000174791. [Q13671-1]
GeneIDi9610.
KEGGihsa:9610.
UCSCiuc001ohn.2. human. [Q13671-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRIN1_HUMAN
AccessioniPrimary (citable) accession number: Q13671
Secondary accession number(s): O15010, Q00427, Q96CC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 17, 2006
Last modified: May 10, 2017
This is version 159 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families