Q13643 (FHL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Four and a half LIM domains protein 3
Short name=FHL-3

Alternative name(s):
Skeletal muscle LIM-protein 2
Short name=SLIM-2

Gene names

Name:	FHL3
Synonyms:	SLIM2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Tissue specificity	Expressed only in skeletal muscle.
Sequence similarities	Contains 4 LIM zinc-binding domains.

Ontologies

Keywords
Domain	LIM domain Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: Compara muscle organ development Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Z disc Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara stress fiber Inferred from electronic annotation. Source: Compara
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 280

280

Four and a half LIM domains protein 3

PRO_0000075740

Regions

Domain

40 – 92

53

LIM zinc-binding 1

Domain

101 – 153

53

LIM zinc-binding 2

Domain

162 – 212

51

LIM zinc-binding 3

Domain

221 – 275

55

LIM zinc-binding 4

Zinc finger

7 – 31

25

C4-type Potential

Experimental info

Sequence conflict

82

1

C → R in AAC04466. Ref.1

Sequence conflict

130

1

S → I in AAC04466. Ref.1

Sequence conflict

140

1

S → P in AAC04466. Ref.1

Sequence conflict

157

1

K → N in AAC04466. Ref.1

Sequence conflict

166

1

S → T in AAC04466. Ref.1

Sequence conflict

174

1

V → L in AAC04466. Ref.1

Sequence conflict

179

1

Q → L in AAC04466. Ref.1

Sequence conflict

183 – 184

2

RE → PK in AAC04466. Ref.1

Sequence conflict

250 – 252

3

SCA → TCD in AAC04466. Ref.1

Sequence conflict

252

1

A → D in CAG33706. Ref.4

Sequence conflict

256

1

T → N in AAC04466. Ref.1

Secondary structure

1

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280

Helix Strand Turn

Details...

Turn

41 – 43

3

Turn

63 – 65

3

Turn

69 – 71

3

Beta strand

80 – 83

4

Beta strand

86 – 89

4

Turn

90 – 92

3

Helix

97 – 99

3

Beta strand

102 – 104

3

Beta strand

110 – 112

3

Turn

124 – 126

3

Turn

130 – 132

3

Turn

136 – 138

3

Beta strand

141 – 144

4

Beta strand

147 – 150

4

Helix

151 – 157

7

Turn

163 – 165

3

Beta strand

174 – 181

8

Turn

183 – 185

3

Beta strand

189 – 191

3

Beta strand

200 – 202

3

Beta strand

204 – 209

6

Helix

210 – 216

7

Sequences

	Sequence		Length	Mass (Da)
	Q13643 [UniParc]. Last modified October 14, 2008. Version 4. Checksum: D1A037C260370DFD	FASTA	280	31,192
	10 20 30 40 50 60 MSESFDCAKC NESLYGRKYI QTDSGPYCVP CYDNTFANTC AECQQLIGHD SRELFYEDRH 70 80 90 100 110 120 FHEGCFRCCR CQRSLADEPF TCQDSELLCN DCYCSAFSSQ CSACGETVMP GSRKLEYGGQ 130 140 150 160 170 180 TWHEHCFLCS GCEQPLGSRS FVPDKGAHYC VPCYENKFAP RCARCSKTLT QGGVTYRDQP 190 200 210 220 230 240 WHRECLVCTG CQTPLAGQQF TSRDEDPYCV ACFGELFAPK CSSCKRPIVG LGGGKYVSFE 250 260 270 280 DRHWHHNCFS CARCSTSLVG QGFVPDGDQV LCQGCSQAGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle." Morgan M.J., Madgwick A.J.A. Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[8]	"Slim defines a novel family of LIM-proteins expressed in skeletal muscle." Morgan M.J., Madgwick A.J.A. Biochem. Biophys. Res. Commun. 225:632-638(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 16-280. Tissue: Skeletal muscle.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Solution structure of LIM domains from human skeletal muscle LIM-protein 2." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 98-218 IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U60116 mRNA. Translation: AAC04466.2.
AK290641 mRNA. Translation: BAF83330.1.
BT007052 mRNA. Translation: AAP35701.1.
CR457425 mRNA. Translation: CAG33706.1.
AL603790 Genomic DNA. Translation: CAH69931.1.
CH471059 Genomic DNA. Translation: EAX07301.1.
CH471059 Genomic DNA. Translation: EAX07302.1.
BC001351 mRNA. Translation: AAH01351.1.
BC011697 mRNA. Translation: AAH11697.1.

IPI

IPI00014399.

PIR

T09504.

RefSeq

NP_001230807.1. NM_001243878.1.
NP_004459.2. NM_004468.4.

UniGene

Hs.57687.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WYH	NMR	-	A	101-159	[»]
2CUQ	NMR	-	A	152-218	[»]
2EHE	NMR	-	A	30-99	[»]

ProteinModelPortal

Q13643.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13643. 13 interactions.

MINT

MINT-155453.

STRING

9606.ENSP00000362107.

PTM databases

PhosphoSite

Q13643.

Polymorphism databases

DMDM

209572768.

Proteomic databases

PaxDb

Q13643.

PRIDE

Q13643.

Protocols and materials databases

DNASU

2275.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000373016; ENSP00000362107; ENSG00000183386.

GeneID

2275.

KEGG

hsa:2275.

UCSC

uc001cck.3. human.

Organism-specific databases

CTD

2275.

GeneCards

GC01M038462.

HGNC

HGNC:3704. FHL3.

HPA

HPA045723.

MIM

602790. gene.

neXtProt

NX_Q13643.

PharmGKB

PA28142.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG314122.

HOGENOM

HOG000231075.

HOVERGEN

HBG074526.

InParanoid

Q13643.

OMA

QFTSRDD.

OrthoDB

EOG46144B.

PhylomeDB

Q13643.

Gene expression databases

ArrayExpress

Q13643.

Bgee

Q13643.

CleanEx

HS_FHL3.

Genevestigator

Q13643.

GermOnline

ENSG00000183386. Homo sapiens.

Family and domain databases

Gene3D

2.10.110.10. 5 hits.

InterPro

IPR001781. Znf_LIM.
[Graphical view]

Pfam

PF00412. LIM. 4 hits.
[Graphical view]

SMART

SM00132. LIM. 4 hits.
[Graphical view]

PROSITE

PS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

FHL3. human.

EvolutionaryTrace

Q13643.

GenomeRNAi

2275.

NextBio

9257.

SOURCE

Search...

Entry information

Entry name

FHL3_HUMAN

Accession

Primary (citable) accession number: Q13643
Secondary accession number(s): D3DPT6, Q6I9T0, Q9BVA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 114 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.