Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trophoblast glycoprotein

Gene

TPBG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.1 Publication

GO - Biological processi

  • cell adhesion Source: ProtInc
  • positive regulation of synapse assembly Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000146242-MONOMER.
SignaLinkiQ13641.

Names & Taxonomyi

Protein namesi
Recommended name:
Trophoblast glycoprotein
Alternative name(s):
5T4 oncofetal antigen
5T4 oncofetal trophoblast glycoprotein
Short name:
5T4 oncotrophoblast glycoprotein
M6P1
Wnt-activated inhibitory factor 1
Short name:
WAIF1
Gene namesi
Name:TPBG
Synonyms:5T4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:12004. TPBG.

Subcellular locationi

  • Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 355ExtracellularSequence analysisAdd BLAST324
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 420CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • endoplasmic reticulum Source: Ensembl
  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76K → A: Strongly reduces Wnt inhibitory function. 1 Publication1
Mutagenesisi97F → T: Strongly reduces Wnt inhibitory function. 1 Publication1
Mutagenesisi124N → Q: Impaired trafficking to the cell surface. 1 Publication1
Mutagenesisi214R → E: Impaired trafficking to the cell surface. 1 Publication1
Mutagenesisi325Y → A: Reduces Wnt inhibitory function. 1 Publication1

Organism-specific databases

DisGeNETi7162.
OpenTargetsiENSG00000146242.
ENSG00000283085.
PharmGKBiPA36685.

Polymorphism and mutation databases

BioMutaiTPBG.
DMDMi73621980.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000001959132 – 420Trophoblast glycoproteinAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi62 ↔ 681 Publication
Disulfide bondi66 ↔ 771 Publication
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi124N-linked (GlcNAc...)1 Publication1
Glycosylationi275N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi298 ↔ 3231 Publication
Disulfide bondi300 ↔ 3441 Publication
Modified residuei418PhosphoserineBy similarity1

Post-translational modificationi

Highly glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ13641.
MaxQBiQ13641.
PaxDbiQ13641.
PeptideAtlasiQ13641.
PRIDEiQ13641.

PTM databases

iPTMnetiQ13641.
PhosphoSitePlusiQ13641.
SwissPalmiQ13641.

Expressioni

Tissue specificityi

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Gene expression databases

BgeeiENSG00000146242.
CleanExiHS_TPBG.
GenevisibleiQ13641. HS.

Organism-specific databases

HPAiHPA010554.

Interactioni

Protein-protein interaction databases

BioGridi113015. 23 interactors.
IntActiQ13641. 1 interactor.
MINTiMINT-230707.
STRINGi9606.ENSP00000358765.

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi67 – 69Combined sources3
Turni70 – 73Combined sources4
Beta strandi74 – 76Combined sources3
Beta strandi94 – 97Combined sources4
Beta strandi104 – 106Combined sources3
Turni108 – 111Combined sources4
Beta strandi122 – 124Combined sources3
Beta strandi130 – 133Combined sources4
Turni135 – 140Combined sources6
Beta strandi146 – 148Combined sources3
Beta strandi155 – 157Combined sources3
Turni159 – 163Combined sources5
Turni164 – 166Combined sources3
Beta strandi177 – 179Combined sources3
Helixi188 – 190Combined sources3
Beta strandi191 – 193Combined sources3
Helixi195 – 204Combined sources10
Helixi205 – 207Combined sources3
Turni208 – 211Combined sources4
Beta strandi214 – 216Combined sources3
Helixi227 – 232Combined sources6
Beta strandi238 – 240Combined sources3
Beta strandi262 – 264Combined sources3
Helixi275 – 281Combined sources7
Beta strandi288 – 290Combined sources3
Helixi300 – 302Combined sources3
Helixi303 – 311Combined sources9
Beta strandi313 – 316Combined sources4
Helixi318 – 320Combined sources3
Beta strandi322 – 326Combined sources5
Helixi327 – 329Combined sources3
Helixi334 – 336Combined sources3
Helixi339 – 341Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CNCX-ray1.77A/B60-345[»]
4CNMX-ray1.75A60-345[»]
ProteinModelPortaliQ13641.
SMRiQ13641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 91LRRNTAdd BLAST39
Repeati92 – 113LRR 11 PublicationAdd BLAST22
Repeati116 – 139LRR 21 PublicationAdd BLAST24
Repeati141 – 163LRR 31 PublicationAdd BLAST23
Repeati172 – 204LRR 41 PublicationAdd BLAST33
Repeati209 – 232LRR 51 PublicationAdd BLAST24
Repeati233 – 255LRR 61 PublicationAdd BLAST23
Repeati256 – 275LRR 71 PublicationAdd BLAST20
Domaini283 – 346LRRCTAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi32 – 53Ser-richAdd BLAST22

Domaini

The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway.1 Publication

Sequence similaritiesi

Contains 7 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00850000132353.
HOGENOMiHOG000013090.
HOVERGENiHBG053843.
InParanoidiQ13641.
OMAiTYVSFRN.
OrthoDBiEOG091G0BIH.
PhylomeDBiQ13641.
TreeFamiTF351115.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF01463. LRRCT. 1 hit.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 6 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q13641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGGCSRGPA AGDGRLRLAR LALVLLGWVS SSSPTSSASS FSSSAPFLAS
60 70 80 90 100
AVSAQPPLPD QCPALCECSE AARTVKCVNR NLTEVPTDLP AYVRNLFLTG
110 120 130 140 150
NQLAVLPAGA FARRPPLAEL AALNLSGSRL DEVRAGAFEH LPSLRQLDLS
160 170 180 190 200
HNPLADLSPF AFSGSNASVS APSPLVELIL NHIVPPEDER QNRSFEGMVV
210 220 230 240 250
AALLAGRALQ GLRRLELASN HFLYLPRDVL AQLPSLRHLD LSNNSLVSLT
260 270 280 290 300
YVSFRNLTHL ESLHLEDNAL KVLHNGTLAE LQGLPHIRVF LDNNPWVCDC
310 320 330 340 350
HMADMVTWLK ETEVVQGKDR LTCAYPEKMR NRVLLELNSA DLDCDPILPP
360 370 380 390 400
SLQTSYVFLG IVLALIGAIF LLVLYLNRKG IKKWMHNIRD ACRDHMEGYH
410 420
YRYEINADPR LTNLSSNSDV
Length:420
Mass (Da):46,032
Last modified:November 1, 1996 - v1
Checksum:i3C403211F40F18BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29083 mRNA. Translation: CAA82324.1.
AK291170 mRNA. Translation: BAF83859.1.
AK074790 mRNA. Translation: BAG52001.1.
CH471051 Genomic DNA. Translation: EAW48683.1.
AL121977 Genomic DNA. Translation: CAI21546.1.
AJ012159 Genomic DNA. Translation: CAA09930.1.
BC037161 mRNA. Translation: AAH37161.1.
CCDSiCCDS4995.1.
PIRiA53531.
RefSeqiNP_001159864.1. NM_001166392.1.
NP_006661.1. NM_006670.4.
XP_011534399.1. XM_011536097.2.
UniGeneiHs.71947.
Hs.82128.

Genome annotation databases

EnsembliENST00000369750; ENSP00000358765; ENSG00000146242.
ENST00000535040; ENSP00000441219; ENSG00000146242.
ENST00000543496; ENSP00000440049; ENSG00000146242.
ENST00000634817; ENSP00000489447; ENSG00000283085.
ENST00000634826; ENSP00000489140; ENSG00000283085.
ENST00000635036; ENSP00000489143; ENSG00000283085.
GeneIDi7162.
KEGGihsa:7162.
UCSCiuc003pjn.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29083 mRNA. Translation: CAA82324.1.
AK291170 mRNA. Translation: BAF83859.1.
AK074790 mRNA. Translation: BAG52001.1.
CH471051 Genomic DNA. Translation: EAW48683.1.
AL121977 Genomic DNA. Translation: CAI21546.1.
AJ012159 Genomic DNA. Translation: CAA09930.1.
BC037161 mRNA. Translation: AAH37161.1.
CCDSiCCDS4995.1.
PIRiA53531.
RefSeqiNP_001159864.1. NM_001166392.1.
NP_006661.1. NM_006670.4.
XP_011534399.1. XM_011536097.2.
UniGeneiHs.71947.
Hs.82128.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CNCX-ray1.77A/B60-345[»]
4CNMX-ray1.75A60-345[»]
ProteinModelPortaliQ13641.
SMRiQ13641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113015. 23 interactors.
IntActiQ13641. 1 interactor.
MINTiMINT-230707.
STRINGi9606.ENSP00000358765.

PTM databases

iPTMnetiQ13641.
PhosphoSitePlusiQ13641.
SwissPalmiQ13641.

Polymorphism and mutation databases

BioMutaiTPBG.
DMDMi73621980.

Proteomic databases

EPDiQ13641.
MaxQBiQ13641.
PaxDbiQ13641.
PeptideAtlasiQ13641.
PRIDEiQ13641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369750; ENSP00000358765; ENSG00000146242.
ENST00000535040; ENSP00000441219; ENSG00000146242.
ENST00000543496; ENSP00000440049; ENSG00000146242.
ENST00000634817; ENSP00000489447; ENSG00000283085.
ENST00000634826; ENSP00000489140; ENSG00000283085.
ENST00000635036; ENSP00000489143; ENSG00000283085.
GeneIDi7162.
KEGGihsa:7162.
UCSCiuc003pjn.5. human.

Organism-specific databases

CTDi7162.
DisGeNETi7162.
GeneCardsiTPBG.
HGNCiHGNC:12004. TPBG.
HPAiHPA010554.
MIMi190920. gene.
neXtProtiNX_Q13641.
OpenTargetsiENSG00000146242.
ENSG00000283085.
PharmGKBiPA36685.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00850000132353.
HOGENOMiHOG000013090.
HOVERGENiHBG053843.
InParanoidiQ13641.
OMAiTYVSFRN.
OrthoDBiEOG091G0BIH.
PhylomeDBiQ13641.
TreeFamiTF351115.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000146242-MONOMER.
SignaLinkiQ13641.

Miscellaneous databases

GeneWikiiTPBG.
GenomeRNAii7162.
PROiQ13641.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000146242.
CleanExiHS_TPBG.
GenevisibleiQ13641. HS.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF01463. LRRCT. 1 hit.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 6 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPBG_HUMAN
AccessioniPrimary (citable) accession number: Q13641
Secondary accession number(s): A8K555
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Antigen 5T4 is overexpressed by a wide spectrum of cancers, including colorectal, ovarian and gastric, but with a limited normal tissue expression. Could be used for tumor immunotherapy.
Reduction of TPBG levels by siRNA significantly enhanced the beta-catenin/TCF transcription-based reporter pBAR activation in response to Wnt stimulation.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.