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Q13641 (TPBG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trophoblast glycoprotein
Alternative name(s):
5T4 oncofetal antigen
5T4 oncofetal trophoblast glycoprotein
Short name=5T4 oncotrophoblast glycoprotein
M6P1
Gene names
Name:TPBG
Synonyms:5T4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Miscellaneous

Antigen 5T4 is overexpressed by a wide spectrum of cancers, including colorectal, ovarian and gastric, but with a limited normal tissue expression. Could be used for tumor immunotherapy.

Sequence similarities

Contains 6 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 LRRNT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 420389Trophoblast glycoprotein
PRO_0000019591

Regions

Topological domain32 – 355324Extracellular Potential
Transmembrane356 – 37621Helical; Potential
Topological domain377 – 42044Cytoplasmic Potential
Domain53 – 9139LRRNT
Repeat92 – 11322LRR 1
Repeat119 – 14022LRR 2
Repeat143 – 16422LRR 3
Repeat211 – 23222LRR 4
Repeat235 – 25622LRR 5
Repeat259 – 28022LRR 6
Domain294 – 34653LRRCT
Compositional bias32 – 5322Ser-rich

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation1241N-linked (GlcNAc...) Ref.5
Glycosylation2751N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q13641 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3C403211F40F18BC

FASTA42046,032
        10         20         30         40         50         60 
MPGGCSRGPA AGDGRLRLAR LALVLLGWVS SSSPTSSASS FSSSAPFLAS AVSAQPPLPD 

        70         80         90        100        110        120 
QCPALCECSE AARTVKCVNR NLTEVPTDLP AYVRNLFLTG NQLAVLPAGA FARRPPLAEL 

       130        140        150        160        170        180 
AALNLSGSRL DEVRAGAFEH LPSLRQLDLS HNPLADLSPF AFSGSNASVS APSPLVELIL 

       190        200        210        220        230        240 
NHIVPPEDER QNRSFEGMVV AALLAGRALQ GLRRLELASN HFLYLPRDVL AQLPSLRHLD 

       250        260        270        280        290        300 
LSNNSLVSLT YVSFRNLTHL ESLHLEDNAL KVLHNGTLAE LQGLPHIRVF LDNNPWVCDC 

       310        320        330        340        350        360 
HMADMVTWLK ETEVVQGKDR LTCAYPEKMR NRVLLELNSA DLDCDPILPP SLQTSYVFLG 

       370        380        390        400        410        420 
IVLALIGAIF LLVLYLNRKG IKKWMHNIRD ACRDHMEGYH YRYEINADPR LTNLSSNSDV 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein: an antigen associated with metastasis contains leucine rich repeats."
Myers K.A., Rahi-Saund V., Davison M.D., Young J.A., Cheater A.J., Stern P.L.
J. Biol. Chem. 269:9319-9324(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 52-75 AND 199-235.
Tissue: Placenta.
[2]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Muscle.
[4]"Attenuated recombinant vaccinia virus expressing oncofetal antigen (tumor-associated antigen) 5T4 induces active therapy of established tumors."
Mulryan K., Ryan M.G., Myers K.A., Shaw D., Wang W., Kingsman S.M., Stern P.L., Carroll M.W.
Mol. Cancer Ther. 1:1129-1137(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: USE IN TUMOR IMMUNOTHERAPHY.
[5]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z29083 mRNA. Translation: CAA82324.1.
AL121977 Genomic DNA. Translation: CAI21546.1.
AJ012159 Genomic DNA. Translation: CAA09930.1.
BC037161 mRNA. Translation: AAH37161.1.
PIRA53531.
RefSeqNP_001159864.1. NM_001166392.1.
NP_006661.1. NM_006670.4.
XP_005248820.1. XM_005248763.1.
UniGeneHs.82128.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4CNCX-ray1.77A/B60-345[»]
4CNMX-ray1.75A60-345[»]
ProteinModelPortalQ13641.
SMRQ13641. Positions 1-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113015. 24 interactions.
IntActQ13641. 1 interaction.
MINTMINT-230707.
STRING9606.ENSP00000358765.

PTM databases

PhosphoSiteQ13641.

Polymorphism databases

DMDM73621980.

Proteomic databases

PaxDbQ13641.
PeptideAtlasQ13641.
PRIDEQ13641.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000369750; ENSP00000358765; ENSG00000146242.
ENST00000535040; ENSP00000441219; ENSG00000146242.
ENST00000543496; ENSP00000440049; ENSG00000146242.
GeneID7162.
KEGGhsa:7162.
UCSCuc003pjn.4. human.

Organism-specific databases

CTD7162.
GeneCardsGC06P083072.
HGNCHGNC:12004. TPBG.
HPAHPA010554.
MIM190920. gene.
neXtProtNX_Q13641.
PharmGKBPA36685.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG301461.
HOGENOMHOG000013090.
HOVERGENHBG053843.
InParanoidQ13641.
OMASFRNLTH.
OrthoDBEOG7QNVM8.
PhylomeDBQ13641.
TreeFamTF351115.

Enzyme and pathway databases

SignaLinkQ13641.

Gene expression databases

ArrayExpressQ13641.
BgeeQ13641.
CleanExHS_TPBG.
GenevestigatorQ13641.

Family and domain databases

InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
[Graphical view]
PfamPF01463. LRRCT. 1 hit.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEPS51450. LRR. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTPBG.
GenomeRNAi7162.
NextBio28026.
PROQ13641.
SOURCESearch...

Entry information

Entry nameTPBG_HUMAN
AccessionPrimary (citable) accession number: Q13641
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM