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Q13637

- RAB32_HUMAN

UniProt

Q13637 - RAB32_HUMAN

Protein

Ras-related protein Rab-32

Gene

RAB32

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 398GTPBy similarity
    Nucleotide bindingi81 – 855GTPBy similarity
    Nucleotide bindingi143 – 1464GTPBy similarity

    GO - Molecular functioni

    1. AP-1 adaptor complex binding Source: BHF-UCL
    2. AP-3 adaptor complex binding Source: BHF-UCL
    3. GTP binding Source: BHF-UCL
    4. GTP-dependent protein binding Source: BHF-UCL
    5. protein binding Source: BHF-UCL
    6. protein complex binding Source: BHF-UCL

    GO - Biological processi

    1. antigen processing and presentation Source: UniProt
    2. endosome to melanosome transport Source: BHF-UCL
    3. melanosome organization Source: BHF-UCL
    4. phagosome maturation Source: UniProtKB
    5. protein transport Source: InterPro
    6. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-32
    Gene namesi
    Name:RAB32
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:9772. RAB32.

    Subcellular locationi

    Mitochondrion. Cytoplasmic vesiclephagosome. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
    Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.

    GO - Cellular componenti

    1. early endosome Source: BHF-UCL
    2. melanosome Source: BHF-UCL
    3. membrane Source: BHF-UCL
    4. mitochondrion Source: UniProtKB-SubCell
    5. phagocytic vesicle Source: UniProtKB
    6. phagocytic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane, Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi39 – 391T → N: Decreased GTP-binding activity. 1 Publication
    Mutagenesisi85 – 851Q → L: No change in GTPase activity. 1 Publication
    Mutagenesisi185 – 1851A → F: Abolishes binding to protein kinase A type II regulatory subunit. 1 Publication
    Mutagenesisi188 – 1881L → P: Abolishes binding to protein kinase A type II regulatory subunit. 1 Publication

    Organism-specific databases

    PharmGKBiPA34123.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 225224Ras-related protein Rab-32PRO_0000121235Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine2 Publications
    Lipidationi224 – 2241S-geranylgeranyl cysteineBy similarity
    Lipidationi225 – 2251S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Prenylation

    Proteomic databases

    MaxQBiQ13637.
    PaxDbiQ13637.
    PRIDEiQ13637.

    PTM databases

    PhosphoSiteiQ13637.

    Expressioni

    Tissue specificityi

    Widely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal lung.2 Publications

    Gene expression databases

    BgeeiQ13637.
    CleanExiHS_RAB32.
    GenevestigatoriQ13637.

    Organism-specific databases

    HPAiHPA025731.

    Interactioni

    Protein-protein interaction databases

    BioGridi116177. 3 interactions.
    STRINGi9606.ENSP00000356465.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4CYMX-ray2.80A/B/C1-225[»]
    4CZ2X-ray2.97A/B/C1-225[»]
    ProteinModelPortaliQ13637.
    SMRiQ13637. Positions 23-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni178 – 19720PKA-RII subunit binding domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi54 – 629Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiQ13637.
    KOiK07918.
    OMAiFCREGGF.
    OrthoDBiEOG78M02X.
    PhylomeDBiQ13637.
    TreeFamiTF324491.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q13637-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGGGAGDPG LGAAAAPAPE TREHLFKVLV IGELGVGKTS IIKRYVHQLF    50
    SQHYRATIGV DFALKVLNWD SRTLVRLQLW DIAGQERFGN MTRVYYKEAV 100
    GAFVVFDISR SSTFEAVLKW KSDLDSKVHL PNGSPIPAVL LANKCDQNKD 150
    SSQSPSQVDQ FCKEHGFAGW FETSAKDNIN IEEAARFLVE KILVNHQSFP 200
    NEENDVDKIK LDQETLRAEN KSQCC 225
    Length:225
    Mass (Da):24,997
    Last modified:January 23, 2007 - v3
    Checksum:i91D41BAC1E3434CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U71127 mRNA. Translation: AAB09599.1.
    AF498958 mRNA. Translation: AAM21106.1.
    BT020016 mRNA. Translation: AAV38819.1.
    AL133539 Genomic DNA. Translation: CAC34968.1.
    BC015061 mRNA. Translation: AAH15061.1.
    U59878 mRNA. Translation: AAB02833.1.
    CCDSiCCDS5210.1.
    RefSeqiNP_006825.1. NM_006834.3.
    UniGeneiHs.287714.

    Genome annotation databases

    EnsembliENST00000367495; ENSP00000356465; ENSG00000118508.
    GeneIDi10981.
    KEGGihsa:10981.
    UCSCiuc003qln.1. human.

    Polymorphism databases

    DMDMi2833245.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U71127 mRNA. Translation: AAB09599.1 .
    AF498958 mRNA. Translation: AAM21106.1 .
    BT020016 mRNA. Translation: AAV38819.1 .
    AL133539 Genomic DNA. Translation: CAC34968.1 .
    BC015061 mRNA. Translation: AAH15061.1 .
    U59878 mRNA. Translation: AAB02833.1 .
    CCDSi CCDS5210.1.
    RefSeqi NP_006825.1. NM_006834.3.
    UniGenei Hs.287714.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4CYM X-ray 2.80 A/B/C 1-225 [» ]
    4CZ2 X-ray 2.97 A/B/C 1-225 [» ]
    ProteinModelPortali Q13637.
    SMRi Q13637. Positions 23-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116177. 3 interactions.
    STRINGi 9606.ENSP00000356465.

    PTM databases

    PhosphoSitei Q13637.

    Polymorphism databases

    DMDMi 2833245.

    Proteomic databases

    MaxQBi Q13637.
    PaxDbi Q13637.
    PRIDEi Q13637.

    Protocols and materials databases

    DNASUi 10981.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000367495 ; ENSP00000356465 ; ENSG00000118508 .
    GeneIDi 10981.
    KEGGi hsa:10981.
    UCSCi uc003qln.1. human.

    Organism-specific databases

    CTDi 10981.
    GeneCardsi GC06P146906.
    HGNCi HGNC:9772. RAB32.
    HPAi HPA025731.
    MIMi 612906. gene.
    neXtProti NX_Q13637.
    PharmGKBi PA34123.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi Q13637.
    KOi K07918.
    OMAi FCREGGF.
    OrthoDBi EOG78M02X.
    PhylomeDBi Q13637.
    TreeFami TF324491.

    Miscellaneous databases

    GenomeRNAii 10981.
    NextBioi 41722.
    PROi Q13637.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q13637.
    CleanExi HS_RAB32.
    Genevestigatori Q13637.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of novel Rab proteins with the yeast two-hybrid system."
      Burke S., Seabra M.C.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. Bienvenut W.V., Claeys D.
      Submitted (NOV-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-22; 28-38; 66-72; 77-87; 111-119; 128-144; 164-186 AND 211-217, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Platelet.
    7. "Molecular cloning, bacterial expression and properties of Rab31 and Rab32."
      Bao X., Faris A.E., Jang E.K., Haslam R.J.
      Eur. J. Biochem. 269:259-271(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-225, TISSUE SPECIFICITY, MUTAGENESIS OF GLN-85.
      Tissue: Platelet.
    8. "Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics."
      Alto N.M., Soderling J., Scott J.D.
      J. Cell Biol. 158:659-668(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-39; ALA-185 AND LEU-188.
    9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes."
      Seto S., Tsujimura K., Koide Y.
      Traffic 12:407-420(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    13. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAB32_HUMAN
    AccessioniPrimary (citable) accession number: Q13637
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3