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Q13636

- RAB31_HUMAN

UniProt

Q13636 - RAB31_HUMAN

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Protein

Ras-related protein Rab-31

Gene

RAB31

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198GTP
Nucleotide bindingi60 – 645GTP
Nucleotide bindingi118 – 1214GTP

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTPase activity Source: ProtInc
  3. GTP binding Source: UniProtKB

GO - Biological processi

  1. cellular response to insulin stimulus Source: UniProtKB
  2. Golgi to plasma membrane protein transport Source: UniProtKB
  3. GTP catabolic process Source: GOC
  4. phagosome maturation Source: UniProtKB
  5. receptor internalization Source: UniProtKB
  6. regulated secretory pathway Source: UniProtKB
  7. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-31
Alternative name(s):
Ras-related protein Rab-22B
Gene namesi
Name:RAB31
Synonyms:RAB22B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:9771. RAB31.

Subcellular locationi

Golgi apparatustrans-Golgi network. Golgi apparatustrans-Golgi network membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Early endosome. Cytoplasmic vesiclephagosome. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Note: Rapidly recruited to phagosomes containing S.aureus or M.tuberculosis.

GO - Cellular componenti

  1. early endosome Source: UniProtKB
  2. phagocytic vesicle Source: UniProtKB
  3. trans-Golgi network membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi64 – 641Q → L: No change in GTPase activity. 1 Publication

Organism-specific databases

PharmGKBiPA34122.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Ras-related protein Rab-31PRO_0000121232Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351Phosphoserine1 Publication
Lipidationi193 – 1931S-geranylgeranyl cysteineBy similarity
Lipidationi194 – 1941S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ13636.
PaxDbiQ13636.
PRIDEiQ13636.

PTM databases

PhosphoSiteiQ13636.

Expressioni

Tissue specificityi

Highest expression in placenta and brain with lower levels in heart and lung. Not detected in liver, skeletal muscle, kidney or pancreas.1 Publication

Gene expression databases

BgeeiQ13636.
CleanExiHS_RAB31.
ExpressionAtlasiQ13636. baseline and differential.
GenevestigatoriQ13636.

Organism-specific databases

HPAiHPA019717.

Interactioni

Subunit structurei

Interacts with OCRL. Interacts with NGFR (By similarity). Interacts (in GDP-bound form) with RIN3 and GAPVD1, which function as guanine exchange factors (GEF). Interacts (in GTP-bound form) with EEA1. Interacts with EGFR.By similarity4 Publications

Protein-protein interaction databases

BioGridi116221. 27 interactions.
IntActiQ13636. 3 interactions.
MINTiMINT-1404598.
STRINGi9606.ENSP00000304565.

Structurei

Secondary structure

1
194
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 129Combined sources
Helixi18 – 2710Combined sources
Beta strandi38 – 4811Combined sources
Beta strandi50 – 6112Combined sources
Helixi65 – 717Combined sources
Helixi72 – 754Combined sources
Beta strandi79 – 868Combined sources
Helixi91 – 10616Combined sources
Beta strandi112 – 1187Combined sources
Helixi120 – 1256Combined sources
Helixi130 – 1389Combined sources
Turni139 – 1413Combined sources
Beta strandi143 – 1464Combined sources
Turni149 – 1524Combined sources
Helixi155 – 16410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FG5X-ray2.80A2-174[»]
ProteinModelPortaliQ13636.
SMRiQ13636. Positions 3-167.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13636.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 429Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000119101.
HOVERGENiHBG009351.
InParanoidiQ13636.
KOiK07891.
OMAiIKLGKQT.
OrthoDBiEOG77DJ7M.
PhylomeDBiQ13636.
TreeFamiTF331262.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13636-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIRELKVCL LGDTGVGKSS IVCRFVQDHF DHNISPTIGA SFMTKTVPCG
60 70 80 90 100
NELHKFLIWD TAGQERFHSL APMYYRGSAA AVIVYDITKQ DSFYTLKKWV
110 120 130 140 150
KELKEHGPEN IVMAIAGNKC DLSDIREVPL KDAKEYAESI GAIVVETSAK
160 170 180 190
NAINIEELFQ GISRQIPPLD PHENGNNGTI KVEKPTMQAS RRCC
Length:194
Mass (Da):21,569
Last modified:November 1, 1997 - v1
Checksum:i19825648A9C214B9
GO

Sequence cautioni

The sequence AAG09690.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH01148.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti189 – 1891A → S in AAC50773. (PubMed:8863739)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57091 mRNA. Translation: AAC50773.1.
U59877 mRNA. Translation: AAB02832.1.
AF234995 mRNA. Translation: AAG13847.1.
AF183421 mRNA. Translation: AAG09690.1. Different initiation.
AF498957 mRNA. Translation: AAM21105.1.
AK314809 mRNA. Translation: BAG37334.1.
BT020027 mRNA. Translation: AAV38830.1.
BT020028 mRNA. Translation: AAV38831.1.
CR407659 mRNA. Translation: CAG28587.1.
AC006238 Genomic DNA. No translation available.
AP000902 Genomic DNA. No translation available.
BC001148 mRNA. Translation: AAH01148.1. Different initiation.
PIRiJC4961.
RefSeqiNP_006859.2. NM_006868.3.
UniGeneiHs.744887.
Hs.99528.

Genome annotation databases

EnsembliENST00000578921; ENSP00000461945; ENSG00000168461.
GeneIDi11031.
KEGGihsa:11031.
UCSCiuc002kog.2. human.

Polymorphism databases

DMDMi2500069.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57091 mRNA. Translation: AAC50773.1 .
U59877 mRNA. Translation: AAB02832.1 .
AF234995 mRNA. Translation: AAG13847.1 .
AF183421 mRNA. Translation: AAG09690.1 . Different initiation.
AF498957 mRNA. Translation: AAM21105.1 .
AK314809 mRNA. Translation: BAG37334.1 .
BT020027 mRNA. Translation: AAV38830.1 .
BT020028 mRNA. Translation: AAV38831.1 .
CR407659 mRNA. Translation: CAG28587.1 .
AC006238 Genomic DNA. No translation available.
AP000902 Genomic DNA. No translation available.
BC001148 mRNA. Translation: AAH01148.1 . Different initiation.
PIRi JC4961.
RefSeqi NP_006859.2. NM_006868.3.
UniGenei Hs.744887.
Hs.99528.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FG5 X-ray 2.80 A 2-174 [» ]
ProteinModelPortali Q13636.
SMRi Q13636. Positions 3-167.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116221. 27 interactions.
IntActi Q13636. 3 interactions.
MINTi MINT-1404598.
STRINGi 9606.ENSP00000304565.

PTM databases

PhosphoSitei Q13636.

Polymorphism databases

DMDMi 2500069.

Proteomic databases

MaxQBi Q13636.
PaxDbi Q13636.
PRIDEi Q13636.

Protocols and materials databases

DNASUi 11031.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000578921 ; ENSP00000461945 ; ENSG00000168461 .
GeneIDi 11031.
KEGGi hsa:11031.
UCSCi uc002kog.2. human.

Organism-specific databases

CTDi 11031.
GeneCardsi GC18P009701.
HGNCi HGNC:9771. RAB31.
HPAi HPA019717.
MIMi 605694. gene.
neXtProti NX_Q13636.
PharmGKBi PA34122.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000119101.
HOVERGENi HBG009351.
InParanoidi Q13636.
KOi K07891.
OMAi IKLGKQT.
OrthoDBi EOG77DJ7M.
PhylomeDBi Q13636.
TreeFami TF331262.

Miscellaneous databases

ChiTaRSi RAB31. human.
EvolutionaryTracei Q13636.
GeneWikii RAB31.
GenomeRNAii 11031.
NextBioi 41922.
PROi Q13636.
SOURCEi Search...

Gene expression databases

Bgeei Q13636.
CleanExi HS_RAB31.
ExpressionAtlasi Q13636. baseline and differential.
Genevestigatori Q13636.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two novel rab genes from human melanocytes."
    Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.
    Gene 174:129-134(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Melanocyte.
  2. "Molecular cloning, bacterial expression and properties of Rab31 and Rab32."
    Bao X., Faris A.E., Jang E.K., Haslam R.J.
    Eur. J. Biochem. 269:259-271(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, MUTAGENESIS OF GLN-64.
    Tissue: Platelet.
  3. "Small GTPase Rab22B is expressed in intestinal epithelial Caco-2 cells."
    Opdam F.J.M., van den Vorstenbosch R., Booltink E., Fransen J.A.M.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "A novel gene expressed in human pheochromocytoma."
    Peng Y., Gu Y., Tu Y., Xu S., Han Z., Fu G., Chen Z.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pheochromocytoma.
  5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  11. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "Rab22B's role in trans-Golgi network membrane dynamics."
    Ng E.L., Wang Y., Tang B.L.
    Biochem. Biophys. Res. Commun. 361:751-757(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  13. "Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes."
    Lodhi I.J., Chiang S.-H., Chang L., Vollenweider D., Watson R.T., Inoue M., Pessin J.E., Saltiel A.R.
    Cell Metab. 5:59-72(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GAPVD1 AND EEA1, SUBCELLULAR LOCATION.
  14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  15. "Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells."
    Ng E.L., Ng J.J., Liang F., Tang B.L.
    J. Cell. Physiol. 221:716-728(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH EGFR AND EEA1.
  16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31."
    Kajiho H., Sakurai K., Minoda T., Yoshikawa M., Nakagawa S., Fukushima S., Kontani K., Katada T.
    J. Biol. Chem. 286:24364-24373(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RIN3.
  18. "Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes."
    Seto S., Tsujimura K., Koide Y.
    Traffic 12:407-420(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  19. "Crystal structure of human RAB31 in complex with a GTP analogue."
    Structural genomics consortium (SGC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 2-174 IN COMPLEX WITH GTP ANALOG.

Entry informationi

Entry nameiRAB31_HUMAN
AccessioniPrimary (citable) accession number: Q13636
Secondary accession number(s): B2RBT7, Q15770, Q9HC00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3