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Q13636 (RAB31_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-31
Alternative name(s):
Ras-related protein Rab-22B
Gene names
Name:RAB31
Synonyms:RAB22B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Ref.12 Ref.13 Ref.15 Ref.17 Ref.18

Subunit structure

Interacts with OCRL. Interacts with NGFR By similarity. Interacts (in GDP-bound form) with RIN3 and GAPVD1, which function as guanine exchange factors (GEF). Interacts (in GTP-bound form) with EEA1. Interacts with EGFR. Ref.13 Ref.15 Ref.17

Subcellular location

Golgi apparatustrans-Golgi network. Golgi apparatustrans-Golgi network membrane; Lipid-anchor; Cytoplasmic side Probable. Early endosome. Cytoplasmic vesiclephagosome. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Rapidly recruited to phagosomes containing S.aureus or M.tuberculosis. Ref.12 Ref.13 Ref.15 Ref.17 Ref.18

Tissue specificity

Highest expression in placenta and brain with lower levels in heart and lung. Not detected in liver, skeletal muscle, kidney or pancreas. Ref.2

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence caution

The sequence AAG09690.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAH01148.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 194194Ras-related protein Rab-31
PRO_0000121232

Regions

Nucleotide binding12 – 198GTP
Nucleotide binding60 – 645GTP
Nucleotide binding118 – 1214GTP
Motif34 – 429Effector region By similarity

Amino acid modifications

Modified residue351Phosphoserine Ref.14
Lipidation1931S-geranylgeranyl cysteine By similarity
Lipidation1941S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis641Q → L: No change in GTPase activity. Ref.2
Sequence conflict1891A → S in AAC50773. Ref.1

Secondary structure

............................. 194
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q13636 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 19825648A9C214B9

FASTA19421,569
        10         20         30         40         50         60 
MAIRELKVCL LGDTGVGKSS IVCRFVQDHF DHNISPTIGA SFMTKTVPCG NELHKFLIWD 

        70         80         90        100        110        120 
TAGQERFHSL APMYYRGSAA AVIVYDITKQ DSFYTLKKWV KELKEHGPEN IVMAIAGNKC 

       130        140        150        160        170        180 
DLSDIREVPL KDAKEYAESI GAIVVETSAK NAINIEELFQ GISRQIPPLD PHENGNNGTI 

       190 
KVEKPTMQAS RRCC 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of two novel rab genes from human melanocytes."
Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.
Gene 174:129-134(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Melanocyte.
[2]"Molecular cloning, bacterial expression and properties of Rab31 and Rab32."
Bao X., Faris A.E., Jang E.K., Haslam R.J.
Eur. J. Biochem. 269:259-271(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, MUTAGENESIS OF GLN-64.
Tissue: Platelet.
[3]"Small GTPase Rab22B is expressed in intestinal epithelial Caco-2 cells."
Opdam F.J.M., van den Vorstenbosch R., Booltink E., Fransen J.A.M.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"A novel gene expressed in human pheochromocytoma."
Peng Y., Gu Y., Tu Y., Xu S., Han Z., Fu G., Chen Z.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pheochromocytoma.
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"DNA sequence and analysis of human chromosome 18."
Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. expand/collapse author list , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[11]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Rab22B's role in trans-Golgi network membrane dynamics."
Ng E.L., Wang Y., Tang B.L.
Biochem. Biophys. Res. Commun. 361:751-757(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]"Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes."
Lodhi I.J., Chiang S.-H., Chang L., Vollenweider D., Watson R.T., Inoue M., Pessin J.E., Saltiel A.R.
Cell Metab. 5:59-72(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GAPVD1 AND EEA1, SUBCELLULAR LOCATION.
[14]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells."
Ng E.L., Ng J.J., Liang F., Tang B.L.
J. Cell. Physiol. 221:716-728(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH EGFR AND EEA1.
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31."
Kajiho H., Sakurai K., Minoda T., Yoshikawa M., Nakagawa S., Fukushima S., Kontani K., Katada T.
J. Biol. Chem. 286:24364-24373(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RIN3.
[18]"Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes."
Seto S., Tsujimura K., Koide Y.
Traffic 12:407-420(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[19]"Crystal structure of human RAB31 in complex with a GTP analogue."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 2-174 IN COMPLEX WITH GTP ANALOG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57091 mRNA. Translation: AAC50773.1.
U59877 mRNA. Translation: AAB02832.1.
AF234995 mRNA. Translation: AAG13847.1.
AF183421 mRNA. Translation: AAG09690.1. Different initiation.
AF498957 mRNA. Translation: AAM21105.1.
AK314809 mRNA. Translation: BAG37334.1.
BT020027 mRNA. Translation: AAV38830.1.
BT020028 mRNA. Translation: AAV38831.1.
CR407659 mRNA. Translation: CAG28587.1.
AC006238 Genomic DNA. No translation available.
AP000902 Genomic DNA. No translation available.
BC001148 mRNA. Translation: AAH01148.1. Different initiation.
PIRJC4961.
RefSeqNP_006859.2. NM_006868.3.
UniGeneHs.744887.
Hs.99528.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FG5X-ray2.80A2-174[»]
ProteinModelPortalQ13636.
SMRQ13636. Positions 3-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116221. 18 interactions.
IntActQ13636. 3 interactions.
MINTMINT-1404598.
STRING9606.ENSP00000304565.

PTM databases

PhosphoSiteQ13636.

Polymorphism databases

DMDM2500069.

Proteomic databases

MaxQBQ13636.
PaxDbQ13636.
PRIDEQ13636.

Protocols and materials databases

DNASU11031.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000578921; ENSP00000461945; ENSG00000168461.
GeneID11031.
KEGGhsa:11031.
UCSCuc002kog.2. human.

Organism-specific databases

CTD11031.
GeneCardsGC18P009701.
HGNCHGNC:9771. RAB31.
HPAHPA019717.
MIM605694. gene.
neXtProtNX_Q13636.
PharmGKBPA34122.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOVERGENHBG009351.
InParanoidQ13636.
KOK07891.
OMAIKLGKQT.
OrthoDBEOG77DJ7M.
PhylomeDBQ13636.
TreeFamTF331262.

Gene expression databases

ArrayExpressQ13636.
BgeeQ13636.
CleanExHS_RAB31.
GenevestigatorQ13636.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRAB31. human.
EvolutionaryTraceQ13636.
GeneWikiRAB31.
GenomeRNAi11031.
NextBio41922.
PROQ13636.
SOURCESearch...

Entry information

Entry nameRAB31_HUMAN
AccessionPrimary (citable) accession number: Q13636
Secondary accession number(s): B2RBT7, Q15770, Q9HC00
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM