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Protein

GDP-L-fucose synthase

Gene

TSTA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.1 Publication

Catalytic activityi

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH.

Pathwayi: GDP-L-fucose biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GDP-mannose 4,6 dehydratase (GMDS)
  2. GDP-L-fucose synthase (TSTA3)
This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose, the pathway GDP-L-fucose biosynthesis via de novo pathway and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei114Important for catalytic activityBy similarity1
Sitei116Important for catalytic activityBy similarity1
Active sitei143Proton donor/acceptorBy similarity1
Binding sitei147NADP2 Publications1
Sitei147Lowers pKa of active site TyrBy similarity1
Binding sitei186NADP1 Publication1
Binding sitei194Substrate1 Publication1
Binding sitei208Substrate1 Publication1
Binding sitei215Substrate1 Publication1
Binding sitei277Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 20NADP2 Publications7
Nucleotide bindingi170 – 173NADP2 Publications4

GO - Molecular functioni

  • coenzyme binding Source: InterPro
  • electron carrier activity Source: UniProtKB
  • GDP-4-dehydro-D-rhamnose reductase activity Source: UniProtKB
  • GDP-L-fucose synthase activity Source: UniProtKB
  • isomerase activity Source: Reactome

GO - Biological processi

  • 'de novo' GDP-L-fucose biosynthetic process Source: UniProtKB
  • cytolysis Source: Ensembl
  • GDP-mannose metabolic process Source: UniProtKB
  • leukocyte cell-cell adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciZFISH:HS02591-MONOMER.
ReactomeiR-HSA-6787639. GDP-fucose biosynthesis.
UniPathwayiUPA00128; UER00191.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-L-fucose synthase (EC:1.1.1.271)
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
Protein FX
Red cell NADP(H)-binding protein
Short-chain dehydrogenase/reductase family 4E member 1
Gene namesi
Name:TSTA3
Synonyms:SDR4E1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:12390. TSTA3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7264.
OpenTargetsiENSG00000104522.
ENSG00000278243.
PharmGKBiPA37056.

Polymorphism and mutation databases

BioMutaiTSTA3.
DMDMi13124123.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743501 – 321GDP-L-fucose synthaseAdd BLAST321

Proteomic databases

EPDiQ13630.
MaxQBiQ13630.
PaxDbiQ13630.
PeptideAtlasiQ13630.
PRIDEiQ13630.

2D gel databases

REPRODUCTION-2DPAGEIPI00014361.

PTM databases

iPTMnetiQ13630.
PhosphoSitePlusiQ13630.
SwissPalmiQ13630.

Expressioni

Gene expression databases

BgeeiENSG00000104522.
CleanExiHS_TSTA3.
ExpressionAtlasiQ13630. baseline and differential.
GenevisibleiQ13630. HS.

Organism-specific databases

HPAiHPA023361.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi113115. 32 interactors.
IntActiQ13630. 2 interactors.
STRINGi9606.ENSP00000398803.

Structurei

Secondary structure

1321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Turni14 – 16Combined sources3
Helixi18 – 23Combined sources6
Turni27 – 29Combined sources3
Beta strandi37 – 40Combined sources4
Beta strandi43 – 46Combined sources4
Helixi51 – 60Combined sources10
Beta strandi64 – 68Combined sources5
Turni74 – 77Combined sources4
Beta strandi78 – 80Combined sources3
Helixi83 – 103Combined sources21
Beta strandi107 – 112Combined sources6
Helixi115 – 117Combined sources3
Beta strandi118 – 121Combined sources4
Beta strandi124 – 126Combined sources3
Helixi128 – 130Combined sources3
Beta strandi138 – 140Combined sources3
Helixi141 – 161Combined sources21
Beta strandi164 – 170Combined sources7
Beta strandi172 – 175Combined sources4
Turni182 – 184Combined sources3
Helixi187 – 199Combined sources13
Beta strandi201 – 203Combined sources3
Beta strandi207 – 209Combined sources3
Beta strandi217 – 219Combined sources3
Helixi220 – 232Combined sources13
Beta strandi240 – 242Combined sources3
Helixi246 – 248Combined sources3
Helixi252 – 263Combined sources12
Beta strandi269 – 272Combined sources4
Helixi287 – 292Combined sources6
Helixi301 – 314Combined sources14
Turni315 – 318Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B8WX-ray2.75A/B7-320[»]
4B8ZX-ray2.75A/B/C/D8-320[»]
4BKPX-ray2.70A/B/C/D8-321[»]
4BL5X-ray2.60A/B/C/D/E/F/G/H/I/J/K/L8-321[»]
4E5YX-ray2.37A/B/C/D1-321[»]
ProteinModelPortaliQ13630.
SMRiQ13630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1431. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00390000004681.
HOGENOMiHOG000168011.
HOVERGENiHBG000059.
InParanoidiQ13630.
KOiK02377.
OMAiRMHTAKL.
OrthoDBiEOG091G0CG3.
PhylomeDBiQ13630.
TreeFamiTF314936.

Family and domain databases

CDDicd05239. GDP_FS_SDR_e. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose/colitose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q13630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD
60 70 80 90 100
TAQTRALFEK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHMNDNVLHS
110 120 130 140 150
AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSN FGYSYAKRMI
160 170 180 190 200
DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS
210 220 230 240 250
SGSALTVWGT GNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV
260 270 280 290 300
SIKEAAEAVV EAMDFHGEVT FDTTKSDGQF KKTASNSKLR TYLPDFRFTP
310 320
FKQAVKETCA WFTDNYEQAR K
Length:321
Mass (Da):35,893
Last modified:November 1, 1996 - v1
Checksum:i94BB1FF61658007C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5Q → E AA sequence (PubMed:7803801).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58766 mRNA. Translation: AAC50786.1.
AK313560 mRNA. Translation: BAG36334.1.
CH471162 Genomic DNA. Translation: EAW82218.1.
CH471162 Genomic DNA. Translation: EAW82220.1.
CH471162 Genomic DNA. Translation: EAW82222.1.
BC001941 mRNA. Translation: AAH01941.1.
BC093061 mRNA. Translation: AAH93061.1.
CCDSiCCDS6408.1.
RefSeqiNP_001304712.1. NM_001317783.1.
NP_003304.1. NM_003313.3.
XP_005251108.2. XM_005251051.3.
XP_011515571.1. XM_011517269.1.
UniGeneiHs.404119.

Genome annotation databases

EnsembliENST00000425753; ENSP00000398803; ENSG00000104522.
ENST00000529064; ENSP00000435386; ENSG00000104522.
ENST00000612580; ENSP00000478042; ENSG00000278243.
ENST00000633782; ENSP00000487640; ENSG00000278243.
GeneIDi7264.
KEGGihsa:7264.
UCSCiuc003yza.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58766 mRNA. Translation: AAC50786.1.
AK313560 mRNA. Translation: BAG36334.1.
CH471162 Genomic DNA. Translation: EAW82218.1.
CH471162 Genomic DNA. Translation: EAW82220.1.
CH471162 Genomic DNA. Translation: EAW82222.1.
BC001941 mRNA. Translation: AAH01941.1.
BC093061 mRNA. Translation: AAH93061.1.
CCDSiCCDS6408.1.
RefSeqiNP_001304712.1. NM_001317783.1.
NP_003304.1. NM_003313.3.
XP_005251108.2. XM_005251051.3.
XP_011515571.1. XM_011517269.1.
UniGeneiHs.404119.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B8WX-ray2.75A/B7-320[»]
4B8ZX-ray2.75A/B/C/D8-320[»]
4BKPX-ray2.70A/B/C/D8-321[»]
4BL5X-ray2.60A/B/C/D/E/F/G/H/I/J/K/L8-321[»]
4E5YX-ray2.37A/B/C/D1-321[»]
ProteinModelPortaliQ13630.
SMRiQ13630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113115. 32 interactors.
IntActiQ13630. 2 interactors.
STRINGi9606.ENSP00000398803.

PTM databases

iPTMnetiQ13630.
PhosphoSitePlusiQ13630.
SwissPalmiQ13630.

Polymorphism and mutation databases

BioMutaiTSTA3.
DMDMi13124123.

2D gel databases

REPRODUCTION-2DPAGEIPI00014361.

Proteomic databases

EPDiQ13630.
MaxQBiQ13630.
PaxDbiQ13630.
PeptideAtlasiQ13630.
PRIDEiQ13630.

Protocols and materials databases

DNASUi7264.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000425753; ENSP00000398803; ENSG00000104522.
ENST00000529064; ENSP00000435386; ENSG00000104522.
ENST00000612580; ENSP00000478042; ENSG00000278243.
ENST00000633782; ENSP00000487640; ENSG00000278243.
GeneIDi7264.
KEGGihsa:7264.
UCSCiuc003yza.3. human.

Organism-specific databases

CTDi7264.
DisGeNETi7264.
GeneCardsiTSTA3.
HGNCiHGNC:12390. TSTA3.
HPAiHPA023361.
MIMi137020. gene.
neXtProtiNX_Q13630.
OpenTargetsiENSG00000104522.
ENSG00000278243.
PharmGKBiPA37056.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1431. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00390000004681.
HOGENOMiHOG000168011.
HOVERGENiHBG000059.
InParanoidiQ13630.
KOiK02377.
OMAiRMHTAKL.
OrthoDBiEOG091G0CG3.
PhylomeDBiQ13630.
TreeFamiTF314936.

Enzyme and pathway databases

UniPathwayiUPA00128; UER00191.
BioCyciZFISH:HS02591-MONOMER.
ReactomeiR-HSA-6787639. GDP-fucose biosynthesis.

Miscellaneous databases

GeneWikiiTSTA3.
GenomeRNAii7264.
PROiQ13630.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104522.
CleanExiHS_TSTA3.
ExpressionAtlasiQ13630. baseline and differential.
GenevisibleiQ13630. HS.

Family and domain databases

CDDicd05239. GDP_FS_SDR_e. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose/colitose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFCL_HUMAN
AccessioniPrimary (citable) accession number: Q13630
Secondary accession number(s): B2R8Y7
, D3DWK5, Q567Q9, Q9UDG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.