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Protein

Apoptosis-stimulating of p53 protein 2

Gene

TP53BP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.3 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • NF-kappaB binding Source: UniProtKB
  • p53 binding Source: AgBase
  • SH3/SH2 adaptor activity Source: ProtInc

GO - Biological processi

Keywordsi

Biological processApoptosis, Cell cycle

Enzyme and pathway databases

ReactomeiR-HSA-139915. Activation of PUMA and translocation to mitochondria.
R-HSA-6803204. TP53 Regulates Transcription of Genes Involved in Cytochrome C Release.
R-HSA-6803205. TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain.
R-HSA-6803211. TP53 Regulates Transcription of Death Receptors and Ligands.
R-HSA-6804759. Regulation of TP53 Activity through Association with Co-factors.
SIGNORiQ13625.

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-stimulating of p53 protein 2
Alternative name(s):
Bcl2-binding protein
Short name:
Bbp
Renal carcinoma antigen NY-REN-51
Tumor suppressor p53-binding protein 2
Short name:
53BP2
Short name:
p53-binding protein 2
Short name:
p53BP2
Gene namesi
Name:TP53BP2
Synonyms:ASPP2, BBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:12000. TP53BP2.

Subcellular locationi

  • Cytoplasmperinuclear region
  • Nucleus

  • Note: Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • mitochondrion Source: GOC
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: AgBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1098W → K: Loss of interaction with APC2. 1 Publication1

Organism-specific databases

DisGeNETi7159.
OpenTargetsiENSG00000143514.
PharmGKBiPA36681.

Polymorphism and mutation databases

BioMutaiTP53BP2.
DMDMi33860140.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669641 – 1128Apoptosis-stimulating of p53 protein 2Add BLAST1128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei480PhosphoserineCombined sources1
Modified residuei556PhosphoserineCombined sources1
Modified residuei569PhosphoserineCombined sources1
Modified residuei572PhosphoserineCombined sources1
Modified residuei576PhosphoserineCombined sources1
Modified residuei698PhosphoserineBy similarity1
Modified residuei714PhosphoserineCombined sources1
Modified residuei737PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13625.
MaxQBiQ13625.
PaxDbiQ13625.
PeptideAtlasiQ13625.
PRIDEiQ13625.

PTM databases

iPTMnetiQ13625.
PhosphoSitePlusiQ13625.

Expressioni

Tissue specificityi

Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.3 Publications

Inductioni

Following DNA damage induced by UV irradiation. Down-regulated by wild-type, but not mutant, p53/TP53.1 Publication

Gene expression databases

BgeeiENSG00000143514.
CleanExiHS_TP53BP2.
ExpressionAtlasiQ13625. baseline and differential.
GenevisibleiQ13625. HS.

Organism-specific databases

HPAiHPA021603.
HPA050429.

Interactioni

Subunit structurei

Binds to the central domain of TP53 as well as to BCL2. Interacts with protein phosphatase 1. Interacts with RELA NF-kappa-B subunit. This interaction probably prevents the activation of apoptosis, possibly by preventing its interaction with TP53. Interacts with APC2 and APPBP1. Interacts with DDX42 (via the C-terminus); the interaction is not inhibited by TP53BP2 ubiquitination and is independent of p53/TP53.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • NF-kappaB binding Source: UniProtKB
  • p53 binding Source: AgBase
  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi113012. 109 interactors.
DIPiDIP-426N.
IntActiQ13625. 113 interactors.
MINTiMINT-106406.
STRINGi9606.ENSP00000341957.

Structurei

Secondary structure

11128
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Beta strandi17 – 22Combined sources6
Helixi29 – 34Combined sources6
Beta strandi40 – 42Combined sources3
Beta strandi44 – 49Combined sources6
Beta strandi52 – 56Combined sources5
Helixi62 – 69Combined sources8
Helixi73 – 75Combined sources3
Beta strandi77 – 81Combined sources5
Helixi747 – 760Combined sources14
Turni761 – 764Combined sources4
Helixi927 – 936Combined sources10
Helixi939 – 945Combined sources7
Helixi946 – 948Combined sources3
Helixi962 – 969Combined sources8
Helixi972 – 981Combined sources10
Helixi995 – 1001Combined sources7
Helixi1005 – 1013Combined sources9
Beta strandi1023 – 1025Combined sources3
Helixi1029 – 1032Combined sources4
Beta strandi1035 – 1037Combined sources3
Helixi1043 – 1053Combined sources11
Turni1054 – 1057Combined sources4
Helixi1058 – 1060Combined sources3
Beta strandi1061 – 1066Combined sources6
Beta strandi1083 – 1086Combined sources4
Beta strandi1091 – 1093Combined sources3
Beta strandi1095 – 1102Combined sources8
Beta strandi1105 – 1110Combined sources6
Helixi1111 – 1113Combined sources3
Beta strandi1114 – 1117Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YCSX-ray2.20B892-1126[»]
2UWQNMR-A1-83[»]
4A63X-ray2.27B/D/F/H/J/L892-1128[»]
4IRVX-ray2.04E/F/G/H726-782[»]
ProteinModelPortaliQ13625.
SMRiQ13625.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13625.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati926 – 957ANK 1Add BLAST32
Repeati958 – 990ANK 2Add BLAST33
Repeati991 – 1024ANK 3Add BLAST34
Repeati1025 – 1067ANK 4Add BLAST43
Domaini1057 – 1119SH3PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni332 – 348Interaction with APPBP11 PublicationAdd BLAST17
Regioni876 – 1128Mediates interaction with APC21 PublicationAdd BLAST253

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi866 – 875SH3-bindingSequence analysis10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi132 – 173Gln-richAdd BLAST42
Compositional biasi824 – 902Pro-richAdd BLAST79

Domaini

The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.

Sequence similaritiesi

Belongs to the ASPP family.Curated

Keywords - Domaini

ANK repeat, Repeat, SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiKOG0515. Eukaryota.
ENOG410Y17W. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000034106.
HOVERGENiHBG050596.
InParanoidiQ13625.
KOiK16823.
OMAiLYQRTTL.
OrthoDBiEOG091G0R1R.
PhylomeDBiQ13625.
TreeFamiTF105545.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR029071. Ubiquitin-rel_dom.
PfamiView protein in Pfam
PF12796. Ank_2. 1 hit.
PF00018. SH3_1. 1 hit.
PRINTSiPR01887. SPECTRNALPHA.
SMARTiView protein in SMART
SM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13625-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMPMFLTVYL SNNEQHFTEV PVTPETICRD VVDLCKEPGE SDCHLAEVWC
60 70 80 90 100
GSERPVADNE RMFDVLQRFG SQRNEVRFFL RHERPPGRDI VSGPRSQDPS
110 120 130 140 150
LKRNGVKVPG EYRRKENGVN SPRMDLTLAE LQEMASRQQQ QIEAQQQLLA
160 170 180 190 200
TKEQRLKFLK QQDQRQQQQV AEQEKLKRLK EIAENQEAKL KKVRALKGHV
210 220 230 240 250
EQKRLSNGKL VEEIEQMNNL FQQKQRELVL AVSKVEELTR QLEMLKNGRI
260 270 280 290 300
DSHHDNQSAV AELDRLYKEL QLRNKLNQEQ NAKLQQQREC LNKRNSEVAV
310 320 330 340 350
MDKRVNELRD RLWKKKAALQ QKENLPVSSD GNLPQQAASA PSRVAAVGPY
360 370 380 390 400
IQSSTMPRMP SRPELLVKPA LPDGSLVIQA SEGPMKIQTL PNMRSGAASQ
410 420 430 440 450
TKGSKIHPVG PDWSPSNADL FPSQGSASVP QSTGNALDQV DDGEVPLREK
460 470 480 490 500
EKKVRPFSMF DAVDQSNAPP SFGTLRKNQS SEDILRDAQV ANKNVAKVPP
510 520 530 540 550
PVPTKPKQIN LPYFGQTNQP PSDIKPDGSS QQLSTVVPSM GTKPKPAGQQ
560 570 580 590 600
PRVLLSPSIP SVGQDQTLSP GSKQESPPAA AVRPFTPQPS KDTLLPPFRK
610 620 630 640 650
PQTVAASSIY SMYTQQQAPG KNFQQAVQSA LTKTHTRGPH FSSVYGKPVI
660 670 680 690 700
AAAQNQQQHP ENIYSNSQGK PGSPEPETEP VSSVQENHEN ERIPRPLSPT
710 720 730 740 750
KLLPFLSNPY RNQSDADLEA LRKKLSNAPR PLKKRSSITE PEGPNGPNIQ
760 770 780 790 800
KLLYQRTTIA AMETISVPSY PSKSASVTAS SESPVEIQNP YLHVEPEKEV
810 820 830 840 850
VSLVPESLSP EDVGNASTEN SDMPAPSPGL DYEPEGVPDN SPNLQNNPEE
860 870 880 890 900
PNPEAPHVLD VYLEEYPPYP PPPYPSGEPE GPGEDSVSMR PPEITGQVSL
910 920 930 940 950
PPGKRTNLRK TGSERIAHGM RVKFNPLALL LDSSLEGEFD LVQRIIYEVD
960 970 980 990 1000
DPSLPNDEGI TALHNAVCAG HTEIVKFLVQ FGVNVNAADS DGWTPLHCAA
1010 1020 1030 1040 1050
SCNNVQVCKF LVESGAAVFA MTYSDMQTAA DKCEEMEEGY TQCSQFLYGV
1060 1070 1080 1090 1100
QEKMGIMNKG VIYALWDYEP QNDDELPMKE GDCMTIIHRE DEDEIEWWWA
1110 1120
RLNDKEGYVP RNLLGLYPRI KPRQRSLA
Length:1,128
Mass (Da):125,616
Last modified:August 15, 2003 - v2
Checksum:iC75D056FBC1DAD75
GO
Isoform 2 (identifier: Q13625-2) [UniParc]FASTAAdd to basket
Also known as: Bbp

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: Due to Alu sequence insertion that creates a shorter but existing form that may have an alternative function.
Show »
Length:1,005
Mass (Da):111,431
Checksum:i3DF4FF49EB589F20
GO
Isoform 3 (identifier: Q13625-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRFGSKM

Note: No experimental confirmation available.
Show »
Length:1,134
Mass (Da):126,323
Checksum:i2C7683F88B171926
GO

Sequence cautioni

The sequence AAH46150 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH46150 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH58918 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0080101 – 123Missing in isoform 2. 1 PublicationAdd BLAST123
Alternative sequenceiVSP_0435091M → MRFGSKM in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58334 mRNA. Translation: AAC50557.1.
AJ318888 mRNA. Translation: CAC83012.1.
AK294432 mRNA. Translation: BAG57677.1.
AC096542 Genomic DNA. No translation available.
BC046150 mRNA. Translation: AAH46150.2. Sequence problems.
BC058918 mRNA. Translation: AAH58918.2. Different initiation.
U09582 mRNA. Translation: AAA21597.1.
CCDSiCCDS1538.1. [Q13625-2]
CCDS44319.1. [Q13625-3]
PIRiI38607.
RefSeqiNP_001026855.2. NM_001031685.2. [Q13625-3]
NP_005417.1. NM_005426.2. [Q13625-2]
XP_011542571.1. XM_011544269.2. [Q13625-2]
UniGeneiHs.523968.

Genome annotation databases

EnsembliENST00000343537; ENSP00000341957; ENSG00000143514. [Q13625-3]
ENST00000391878; ENSP00000375750; ENSG00000143514. [Q13625-2]
GeneIDi7159.
KEGGihsa:7159.
UCSCiuc001hod.4. human. [Q13625-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58334 mRNA. Translation: AAC50557.1.
AJ318888 mRNA. Translation: CAC83012.1.
AK294432 mRNA. Translation: BAG57677.1.
AC096542 Genomic DNA. No translation available.
BC046150 mRNA. Translation: AAH46150.2. Sequence problems.
BC058918 mRNA. Translation: AAH58918.2. Different initiation.
U09582 mRNA. Translation: AAA21597.1.
CCDSiCCDS1538.1. [Q13625-2]
CCDS44319.1. [Q13625-3]
PIRiI38607.
RefSeqiNP_001026855.2. NM_001031685.2. [Q13625-3]
NP_005417.1. NM_005426.2. [Q13625-2]
XP_011542571.1. XM_011544269.2. [Q13625-2]
UniGeneiHs.523968.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YCSX-ray2.20B892-1126[»]
2UWQNMR-A1-83[»]
4A63X-ray2.27B/D/F/H/J/L892-1128[»]
4IRVX-ray2.04E/F/G/H726-782[»]
ProteinModelPortaliQ13625.
SMRiQ13625.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113012. 109 interactors.
DIPiDIP-426N.
IntActiQ13625. 113 interactors.
MINTiMINT-106406.
STRINGi9606.ENSP00000341957.

PTM databases

iPTMnetiQ13625.
PhosphoSitePlusiQ13625.

Polymorphism and mutation databases

BioMutaiTP53BP2.
DMDMi33860140.

Proteomic databases

EPDiQ13625.
MaxQBiQ13625.
PaxDbiQ13625.
PeptideAtlasiQ13625.
PRIDEiQ13625.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343537; ENSP00000341957; ENSG00000143514. [Q13625-3]
ENST00000391878; ENSP00000375750; ENSG00000143514. [Q13625-2]
GeneIDi7159.
KEGGihsa:7159.
UCSCiuc001hod.4. human. [Q13625-1]

Organism-specific databases

CTDi7159.
DisGeNETi7159.
GeneCardsiTP53BP2.
HGNCiHGNC:12000. TP53BP2.
HPAiHPA021603.
HPA050429.
MIMi602143. gene.
neXtProtiNX_Q13625.
OpenTargetsiENSG00000143514.
PharmGKBiPA36681.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0515. Eukaryota.
ENOG410Y17W. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000034106.
HOVERGENiHBG050596.
InParanoidiQ13625.
KOiK16823.
OMAiLYQRTTL.
OrthoDBiEOG091G0R1R.
PhylomeDBiQ13625.
TreeFamiTF105545.

Enzyme and pathway databases

ReactomeiR-HSA-139915. Activation of PUMA and translocation to mitochondria.
R-HSA-6803204. TP53 Regulates Transcription of Genes Involved in Cytochrome C Release.
R-HSA-6803205. TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain.
R-HSA-6803211. TP53 Regulates Transcription of Death Receptors and Ligands.
R-HSA-6804759. Regulation of TP53 Activity through Association with Co-factors.
SIGNORiQ13625.

Miscellaneous databases

ChiTaRSiTP53BP2. human.
EvolutionaryTraceiQ13625.
GeneWikiiTP53BP2.
GenomeRNAii7159.
PROiPR:Q13625.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143514.
CleanExiHS_TP53BP2.
ExpressionAtlasiQ13625. baseline and differential.
GenevisibleiQ13625. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
IPR029071. Ubiquitin-rel_dom.
PfamiView protein in Pfam
PF12796. Ank_2. 1 hit.
PF00018. SH3_1. 1 hit.
PRINTSiPR01887. SPECTRNALPHA.
SMARTiView protein in SMART
SM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASPP2_HUMAN
AccessioniPrimary (citable) accession number: Q13625
Secondary accession number(s): B4DG66
, Q12892, Q86X75, Q96KQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 15, 2003
Last modified: March 15, 2017
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.