Q13591 (SEM5A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Semaphorin-5A

Alternative name(s):
Semaphorin-F
Short name=Sema F

Gene names

Name:	SEMA5A
Synonyms:	SEMAF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as positive axonal guidance cues.
Subunit structure	Binds PLXNB3.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the semaphorin family. Contains 1 PSI domain. Contains 1 Sema domain. Contains 7 TSP type-1 domains.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Traceable author statement. Source: ProtInc cell-cell signaling Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 1074	1052	Semaphorin-5A		PRO_0000032335
Regions
	Topological domain	23 – 968	946	Extracellular Potential
	Transmembrane	969 – 989	21	Helical; Potential
	Topological domain	990 – 1074	85	Cytoplasmic Potential
	Domain	35 – 484	450	Sema
	Domain	540 – 593	54	TSP type-1 1
	Domain	595 – 651	57	TSP type-1 2
	Domain	653 – 702	50	TSP type-1 3
	Domain	707 – 765	59	TSP type-1 4
	Domain	784 – 839	56	TSP type-1 5
	Domain	841 – 896	56	TSP type-1 6
	Domain	897 – 944	48	TSP type-1 7
Amino acid modifications
	Modified residue	106	1	Phosphoserine Ref.6
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	227	1	N-linked (GlcNAc...) Potential
	Glycosylation	277	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential
	Glycosylation	536	1	N-linked (GlcNAc...) Potential
	Glycosylation	591	1	N-linked (GlcNAc...) Potential
	Glycosylation	717	1	N-linked (GlcNAc...) Potential
	Glycosylation	933	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 114		By similarity
	Disulfide bond	131 ↔ 140		By similarity
	Disulfide bond	254 ↔ 357		By similarity
	Disulfide bond	278 ↔ 320		By similarity
	Disulfide bond	487 ↔ 504		By similarity
	Disulfide bond	496 ↔ 513		By similarity
	Disulfide bond	607 ↔ 644		By similarity
	Disulfide bond	611 ↔ 650		By similarity
	Disulfide bond	622 ↔ 634		By similarity
	Disulfide bond	665 ↔ 696		By similarity
	Disulfide bond	669 ↔ 701		By similarity
	Disulfide bond	680 ↔ 686		By similarity
	Disulfide bond	796 ↔ 833		By similarity
	Disulfide bond	800 ↔ 838		By similarity
	Disulfide bond	811 ↔ 823		By similarity
	Disulfide bond	853 ↔ 890		By similarity
	Disulfide bond	857 ↔ 895		By similarity
	Disulfide bond	868 ↔ 880		By similarity
Natural variations
	Natural variant	246	1	V → L. Corresponds to variant rs1806079 [ dbSNP | Ensembl ].		VAR_030294
	Natural variant	792	1	S → L. Corresponds to variant rs2290734 [ dbSNP | Ensembl ].		VAR_030295
Experimental info
	Sequence conflict	56	1	V → A in AAC09473. Ref.1
	Sequence conflict	149	1	T → A in AAC09473. Ref.1
	Sequence conflict	382	1	M → V in AAC09473. Ref.1
	Sequence conflict	494	1	S → R in AAC14668. Ref.2
	Sequence conflict	723	1	G → D in AAC09473. Ref.1
	Sequence conflict	835	1	I → T in AAC09473. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13591 [UniParc]. Last modified May 30, 2006. Version 3. Checksum: AE073413AC974CCC	FASTA	1,074	120,615
	10 20 30 40 50 60 MKGTCVIAWL FSSLGLWRLA HPEAQGTTQC QRTEHPVISY KEIGPWLREF RAKNAVDFSQ 70 80 90 100 110 120 LTFDPGQKEL VVGARNYLFR LQLEDLSLIQ AVEWECDEAT KKACYSKGKS KEECQNYIRV 130 140 150 160 170 180 LLVGGDRLFT CGTNAFTPVC TNRSLSNLTE IHDQISGMAR CPYSPQHNST ALLTAGGELY 190 200 210 220 230 240 AATAMDFPGR DPAIYRSLGI LPPLRTAQYN SKWLNEPNFV SSYDIGNFTY FFFRENAVEH 250 260 270 280 290 300 DCGKTVFSRA ARVCKNDIGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE LQSTFFLPEL 310 320 330 340 350 360 DLIYGIFTTN VNSIAASAVC VFNLSAIAQA FSGPFKYQEN SRSAWLPYPN PNPHFQCGTV 370 380 390 400 410 420 DQGLYVNLTE RNLQDAQKFI LMHEVVQPVT TVPSFMEDNS RFSHVAVDVV QGREALVHII 430 440 450 460 470 480 YLATDYGTIK KVRVPLNQTS SSCLLEEIEL FPERRREPIR SLQILHSQSV LFVGLREHVV 490 500 510 520 530 540 KIPLKRCQFY RTRSTCIGAQ DPYCGWDVVM KKCTSLEESL SMTQWEQSIS ACPTRNLTVD 550 560 570 580 590 600 GHFGVWSPWT PCTHTDGSAV GSCLCRTRSC DSPAPQCGGW QCEGPGMEIA NCSRNGGWTP 610 620 630 640 650 660 WTSWSPCSTT CGIGFQVRQR SCSNPTPRHG GRVCVGQNRE ERYCNEHLLC PPHMFWTGWG 670 680 690 700 710 720 PWERCTAQCG GGIQARRRIC ENGPDCAGCN VEYQSCNTNP CPELKKTTPW TPWTPVNISD 730 740 750 760 770 780 NGGHYEQRFR YTCKARLADP NLLEVGRQRI EMRYCSSDGT SGCSTDGLSG DFLRAGRYSA 790 800 810 820 830 840 HTVNGAWSAW TSWSQCSRDC SRGIRNRKRV CNNPEPKYGG MPCLGPSLEY QECNILPCPV 850 860 870 880 890 900 DGVWSCWSPW TKCSATCGGG HYMRTRSCSN PAPAYGGDIC LGLHTEEALC NTQPCPESWS 910 920 930 940 950 960 EWSDWSECEA SGVQVRARQC ILLFPMGSQC SGNTTESRPC VFDSNFIPEV SVARSSSVEE 970 980 990 1000 1010 1020 KRCGEFNMFH MIAVGLSSSI LGCLLTLLVY TYCQRYQQQS HDATVIHPVS PAPLNTSITN 1030 1040 1050 1060 1070 HINKLDKYDS VEAIKAFNKN NLILEERNKY FNPHLTGKTY SNAYFTDLNN YDEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and mapping of human semaphorin F from the Cri-du-chat candidate interval." Simmons A.D., Puschel A.W., McPherson J.D., Overhauser J., Lovett M. Biochem. Biophys. Res. Commun. 242:685-691(1998) [PubMed: 9464278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Plexin-B3 is a functional receptor for semaphorin 5A." Artigiani S., Conrotto P., Fazzari P., Gilestro G.F., Barberis D., Giordano S., Comoglio P.M., Tamagnone L. EMBO Rep. 5:710-714(2004) [PubMed: 15218527] [Abstract] Cited for: INTERACTION WITH PLXNB3.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52840 mRNA. Translation: AAC09473.1. AC004615 Genomic DNA. Translation: AAC14668.1. AC022446 Genomic DNA. No translation available. AC027336 Genomic DNA. No translation available. AC091906 Genomic DNA. No translation available. CH471102 Genomic DNA. Translation: EAX08078.1. CH471102 Genomic DNA. Translation: EAX08079.1. BC115696 mRNA. Translation: AAI15697.1.
IPI	IPI00013880.
PIR	JC5928.
RefSeq	NP_003957.2. NM_003966.2.
UniGene	Hs.27621.
3D structure databases
ProteinModelPortal	Q13591.
SMR	Q13591. Positions 53-530, 540-645, 648-704, 783-945.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13591. 1 interaction.
STRING	Q13591.
PTM databases
PhosphoSite	Q13591.
Polymorphism databases
DMDM	109939725.
Proteomic databases
PRIDE	Q13591.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000382496; ENSP00000371936; ENSG00000112902.
GeneID	9037.
KEGG	hsa:9037.
NMPDR	fig|9606.3.peg.25042.
UCSC	uc003jek.2. human.
Organism-specific databases
CTD	9037.
GeneCards	GC05M009091.
H-InvDB	HIX0032118.
HGNC	HGNC:10736. SEMA5A.
HPA	HPA004632.
MIM	609297. gene.
neXtProt	NX_Q13591.
Orphanet	281. Monosomy 5p.
PharmGKB	PA35658.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19105.
GeneTree	ENSGT00580000081327.
HOGENOM	HBG355203.
HOVERGEN	HBG062356.
InParanoid	Q13591.
OMA	DSNFIPE.
OrthoDB	EOG4PG603.
PhylomeDB	Q13591.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology.
Gene expression databases
ArrayExpress	Q13591.
Bgee	Q13591.
CleanEx	HS_SEMA5A.
Genevestigator	Q13591.
GermOnline	ENSG00000112902. Homo sapiens.
Family and domain databases
InterPro	IPR003659. Plexin-like. IPR016201. Plexin-like_fold. IPR002165. Plexin_repeat. IPR001627. Semaphorin/CD100_Ag. IPR000884. Thrombospondin_1_rpt. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K06841.
Pfam	PF01437. PSI. 1 hit. PF01403. Sema. 1 hit. PF00090. TSP_1. 5 hits. [Graphical view]
SMART	SM00423. PSI. 1 hit. SM00630. Sema. 1 hit. SM00209. TSP1. 6 hits. [Graphical view]
SUPFAM	SSF103575. Plexin-like_fold. 1 hit. SSF101912. Sema. 1 hit. SSF82895. TSP1. 6 hits.
PROSITE	PS51004. SEMA. 1 hit. PS50092. TSP1. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	33851.
SOURCE	Search...

Entry information

Entry name

SEM5A_HUMAN

Accession

Primary (citable) accession number: Q13591
Secondary accession number(s): D3DTC6, O60408, Q1RLL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2006
Last modified:	December 14, 2011
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.