Q13588 (GRAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GRB2-related adapter protein

Gene names

Name:

GRAP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
Subunit structure	Associates through its SH2 domain with ligand-activated receptors for stem cell factor (KIT) and erythropoietin (EPOR). Also forms a stable complex with the Bcr-Abl oncoprotein. GRAP is associated with the Ras guanine nucleotide exchange factor SOS1, primarily through its N-terminal SH3 domain. Interacts with phosphorylated LAT upon TCR activation. Interacts with SHB. Ref.6 Ref.7
Sequence similarities	Belongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.

Ontologies

Keywords
Domain	Repeat SH2 domain SH3 domain
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Traceable author statement Ref.1. Source: ProtInc cell-cell signaling Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	SH3/SH2 adaptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	GRB2-related adapter protein		PRO_0000088206
Regions
	Domain	1 – 58	58	SH3 1
	Domain	60 – 154	95	SH2
	Domain	158 – 217	60	SH3 2

Sequences

	Sequence		Length	Mass (Da)
	Q13588 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 09FEC2F3BAC0FAF8	FASTA	217	25,337
	10 20 30 40 50 60 MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGVEGFIPK NYIRVKPHPW 70 80 90 100 110 120 YSGRISRQLA EEILMKRNHL GAFLIRESES SPGEFSVSVN YGDQVQHFKV LREASGKYFL 130 140 150 160 170 180 WEEKFNSLNE LVDFYRTTTI AKKRQIFLRD EEPLLKSPGA CFAQAQFDFS AQDPSQLSFR 190 200 210 RGDIIEVLER PDPHWWRGRS CGRVGFFPRS YVQPVHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway." Feng G.-S., Ouyang Y.-B., Hu D.-P., Shi Z.-Q., Gentz R., Ni J. J. Biol. Chem. 271:12129-12132(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood and Brain.
[6]	"LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." Zhang W., Sloan-Lancaster J., Kitchen J., Trible R.P., Samelson L.E. Cell 92:83-92(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LAT.
[7]	"Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins." Welsh M., Songyang Z., Frantz J.D., Trueb T., Reedquist K.A., Karlsson T., Miyazaki M., Cantley L.C., Band H., Shoelson S.E. Oncogene 16:891-901(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SHB.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52518 mRNA. Translation: AAC50541.1. BT019981 mRNA. Translation: AAV38784.1. CR541941 mRNA. Translation: CAG46739.1. CR541966 mRNA. Translation: CAG46764.1. AC003957 Genomic DNA. No translation available. AC007952 Genomic DNA. No translation available. BC035856 mRNA. Translation: AAH35856.1. BC063035 mRNA. Translation: AAH63035.1.
IPI	IPI00013864.
RefSeq	NP_006604.1. NM_006613.3.
UniGene	Hs.567416.
3D structure databases
ProteinModelPortal	Q13588.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13588. 1 interaction.
MINT	MINT-1515623.
STRING	9606.ENSP00000284154.
Polymorphism databases
DMDM	3913785.
Proteomic databases
PaxDb	Q13588.
PRIDE	Q13588.
Protocols and materials databases
DNASU	10750.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000284154; ENSP00000284154; ENSG00000154016.
GeneID	10750.
KEGG	hsa:10750.
UCSC	uc002guy.3. human.
Organism-specific databases
CTD	10750.
GeneCards	GC17M018923.
H-InvDB	HIX0039163.
HGNC	HGNC:4562. GRAP.
HPA	HPA046595.
MIM	604330. gene.
neXtProt	NX_Q13588.
PharmGKB	PA28958.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG298780.
HOGENOM	HOG000251625.
HOVERGEN	HBG005404.
InParanoid	Q13588.
OMA	RACFAQA.
OrthoDB	EOG4Z36FM.
PhylomeDB	Q13588.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
SignaLink	Q13588.
Gene expression databases
ArrayExpress	Q13588.
Bgee	Q13588.
CleanEx	HS_GRAP.
Genevestigator	Q13588.
GermOnline	ENSG00000154016. Homo sapiens.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR000108. p67phox. IPR000980. SH2. IPR011511. SH3_2. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. PF07653. SH3_2. 1 hit. [Graphical view]
PRINTS	PR00499. P67PHOX. PR00401. SH2DOMAIN. PR00452. SH3DOMAIN.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 2 hits. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50001. SH2. 1 hit. PS50002. SH3. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GRAP. human.
GenomeRNAi	10750.
NextBio	40825.
SOURCE	Search...

Entry information

Entry name

GRAP_HUMAN

Accession

Primary (citable) accession number: Q13588

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.