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Protein

GRB2-related adapter protein

Gene

GRAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.

GO - Molecular functioni

  1. SH3/SH2 adaptor activity Source: ProtInc

GO - Biological processi

  1. cell-cell signaling Source: ProtInc
  2. positive regulation of signal transduction Source: GOC
  3. Ras protein signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_111040. Signaling by SCF-KIT.
SignaLinkiQ13588.

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-related adapter protein
Gene namesi
Name:GRAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:4562. GRAP.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: ProtInc
  2. cytosol Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28958.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217GRB2-related adapter proteinPRO_0000088206Add
BLAST

Proteomic databases

MaxQBiQ13588.
PaxDbiQ13588.
PRIDEiQ13588.

Expressioni

Gene expression databases

BgeeiQ13588.
CleanExiHS_GRAP.
ExpressionAtlasiQ13588. baseline and differential.
GenevestigatoriQ13588.

Organism-specific databases

HPAiHPA046595.

Interactioni

Subunit structurei

Associates through its SH2 domain with ligand-activated receptors for stem cell factor (KIT) and erythropoietin (EPOR). Also forms a stable complex with the Bcr-Abl oncoprotein. GRAP is associated with the Ras guanine nucleotide exchange factor SOS1, primarily through its N-terminal SH3 domain. Interacts with phosphorylated LAT upon TCR activation. Interacts with SHB.2 Publications

Protein-protein interaction databases

BioGridi115973. 9 interactions.
IntActiQ13588. 3 interactions.
MINTiMINT-1515623.
STRINGi9606.ENSP00000284154.

Structurei

3D structure databases

ProteinModelPortaliQ13588.
SMRiQ13588. Positions 1-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5858SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 15495SH2PROSITE-ProRule annotationAdd
BLAST
Domaini158 – 21760SH3 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG298780.
GeneTreeiENSGT00550000074482.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiQ13588.
OMAiRACFAQA.
OrthoDBiEOG75F4F6.
PhylomeDBiQ13588.
TreeFamiTF354288.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13588-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGVEGFIPK
60 70 80 90 100
NYIRVKPHPW YSGRISRQLA EEILMKRNHL GAFLIRESES SPGEFSVSVN
110 120 130 140 150
YGDQVQHFKV LREASGKYFL WEEKFNSLNE LVDFYRTTTI AKKRQIFLRD
160 170 180 190 200
EEPLLKSPGA CFAQAQFDFS AQDPSQLSFR RGDIIEVLER PDPHWWRGRS
210
CGRVGFFPRS YVQPVHL
Length:217
Mass (Da):25,337
Last modified:November 1, 1996 - v1
Checksum:i09FEC2F3BAC0FAF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52518 mRNA. Translation: AAC50541.1.
BT019981 mRNA. Translation: AAV38784.1.
CR541941 mRNA. Translation: CAG46739.1.
CR541966 mRNA. Translation: CAG46764.1.
AC003957 Genomic DNA. No translation available.
AC007952 Genomic DNA. No translation available.
BC035856 mRNA. Translation: AAH35856.1.
BC063035 mRNA. Translation: AAH63035.1.
CCDSiCCDS11202.1.
RefSeqiNP_006604.1. NM_006613.3.
UniGeneiHs.567416.

Genome annotation databases

EnsembliENST00000284154; ENSP00000284154; ENSG00000154016.
GeneIDi10750.
KEGGihsa:10750.
UCSCiuc002guy.3. human.

Polymorphism databases

DMDMi3913785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52518 mRNA. Translation: AAC50541.1.
BT019981 mRNA. Translation: AAV38784.1.
CR541941 mRNA. Translation: CAG46739.1.
CR541966 mRNA. Translation: CAG46764.1.
AC003957 Genomic DNA. No translation available.
AC007952 Genomic DNA. No translation available.
BC035856 mRNA. Translation: AAH35856.1.
BC063035 mRNA. Translation: AAH63035.1.
CCDSiCCDS11202.1.
RefSeqiNP_006604.1. NM_006613.3.
UniGeneiHs.567416.

3D structure databases

ProteinModelPortaliQ13588.
SMRiQ13588. Positions 1-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115973. 9 interactions.
IntActiQ13588. 3 interactions.
MINTiMINT-1515623.
STRINGi9606.ENSP00000284154.

Polymorphism databases

DMDMi3913785.

Proteomic databases

MaxQBiQ13588.
PaxDbiQ13588.
PRIDEiQ13588.

Protocols and materials databases

DNASUi10750.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284154; ENSP00000284154; ENSG00000154016.
GeneIDi10750.
KEGGihsa:10750.
UCSCiuc002guy.3. human.

Organism-specific databases

CTDi10750.
GeneCardsiGC17M018923.
H-InvDBHIX0039163.
HGNCiHGNC:4562. GRAP.
HPAiHPA046595.
MIMi604330. gene.
neXtProtiNX_Q13588.
PharmGKBiPA28958.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG298780.
GeneTreeiENSGT00550000074482.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiQ13588.
OMAiRACFAQA.
OrthoDBiEOG75F4F6.
PhylomeDBiQ13588.
TreeFamiTF354288.

Enzyme and pathway databases

ReactomeiREACT_111040. Signaling by SCF-KIT.
SignaLinkiQ13588.

Miscellaneous databases

ChiTaRSiGRAP. human.
GeneWikiiGRAP.
GenomeRNAii10750.
NextBioi40825.
PROiQ13588.
SOURCEiSearch...

Gene expression databases

BgeeiQ13588.
CleanExiHS_GRAP.
ExpressionAtlasiQ13588. baseline and differential.
GenevestigatoriQ13588.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway."
    Feng G.-S., Ouyang Y.-B., Hu D.-P., Shi Z.-Q., Gentz R., Ni J.
    J. Biol. Chem. 271:12129-12132(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood and Brain.
  6. "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation."
    Zhang W., Sloan-Lancaster J., Kitchen J., Trible R.P., Samelson L.E.
    Cell 92:83-92(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAT.
  7. "Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins."
    Welsh M., Songyang Z., Frantz J.D., Trueb T., Reedquist K.A., Karlsson T., Miyazaki M., Cantley L.C., Band H., Shoelson S.E.
    Oncogene 16:891-901(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHB.

Entry informationi

Entry nameiGRAP_HUMAN
AccessioniPrimary (citable) accession number: Q13588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.