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Q13588

- GRAP_HUMAN

UniProt

Q13588 - GRAP_HUMAN

Protein

GRB2-related adapter protein

Gene

GRAP

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.

    GO - Molecular functioni

    1. SH3/SH2 adaptor activity Source: ProtInc

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. positive regulation of signal transduction Source: GOC
    3. Ras protein signal transduction Source: ProtInc

    Enzyme and pathway databases

    ReactomeiREACT_111040. Signaling by SCF-KIT.
    SignaLinkiQ13588.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GRB2-related adapter protein
    Gene namesi
    Name:GRAP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:4562. GRAP.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. cytosol Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28958.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217GRB2-related adapter proteinPRO_0000088206Add
    BLAST

    Proteomic databases

    MaxQBiQ13588.
    PaxDbiQ13588.
    PRIDEiQ13588.

    Expressioni

    Gene expression databases

    ArrayExpressiQ13588.
    BgeeiQ13588.
    CleanExiHS_GRAP.
    GenevestigatoriQ13588.

    Organism-specific databases

    HPAiHPA046595.

    Interactioni

    Subunit structurei

    Associates through its SH2 domain with ligand-activated receptors for stem cell factor (KIT) and erythropoietin (EPOR). Also forms a stable complex with the Bcr-Abl oncoprotein. GRAP is associated with the Ras guanine nucleotide exchange factor SOS1, primarily through its N-terminal SH3 domain. Interacts with phosphorylated LAT upon TCR activation. Interacts with SHB.2 Publications

    Protein-protein interaction databases

    BioGridi115973. 7 interactions.
    IntActiQ13588. 3 interactions.
    MINTiMINT-1515623.
    STRINGi9606.ENSP00000284154.

    Structurei

    3D structure databases

    ProteinModelPortaliQ13588.
    SMRiQ13588. Positions 1-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 5858SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini60 – 15495SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini158 – 21760SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GRB2/sem-5/DRK family.Curated
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 2 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiNOG298780.
    HOGENOMiHOG000251625.
    HOVERGENiHBG005404.
    InParanoidiQ13588.
    OMAiRACFAQA.
    OrthoDBiEOG75F4F6.
    PhylomeDBiQ13588.
    TreeFamiTF354288.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR000980. SH2.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 2 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q13588-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGVEGFIPK    50
    NYIRVKPHPW YSGRISRQLA EEILMKRNHL GAFLIRESES SPGEFSVSVN 100
    YGDQVQHFKV LREASGKYFL WEEKFNSLNE LVDFYRTTTI AKKRQIFLRD 150
    EEPLLKSPGA CFAQAQFDFS AQDPSQLSFR RGDIIEVLER PDPHWWRGRS 200
    CGRVGFFPRS YVQPVHL 217
    Length:217
    Mass (Da):25,337
    Last modified:November 1, 1996 - v1
    Checksum:i09FEC2F3BAC0FAF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52518 mRNA. Translation: AAC50541.1.
    BT019981 mRNA. Translation: AAV38784.1.
    CR541941 mRNA. Translation: CAG46739.1.
    CR541966 mRNA. Translation: CAG46764.1.
    AC003957 Genomic DNA. No translation available.
    AC007952 Genomic DNA. No translation available.
    BC035856 mRNA. Translation: AAH35856.1.
    BC063035 mRNA. Translation: AAH63035.1.
    CCDSiCCDS11202.1.
    RefSeqiNP_006604.1. NM_006613.3.
    UniGeneiHs.567416.

    Genome annotation databases

    EnsembliENST00000284154; ENSP00000284154; ENSG00000154016.
    GeneIDi10750.
    KEGGihsa:10750.
    UCSCiuc002guy.3. human.

    Polymorphism databases

    DMDMi3913785.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52518 mRNA. Translation: AAC50541.1 .
    BT019981 mRNA. Translation: AAV38784.1 .
    CR541941 mRNA. Translation: CAG46739.1 .
    CR541966 mRNA. Translation: CAG46764.1 .
    AC003957 Genomic DNA. No translation available.
    AC007952 Genomic DNA. No translation available.
    BC035856 mRNA. Translation: AAH35856.1 .
    BC063035 mRNA. Translation: AAH63035.1 .
    CCDSi CCDS11202.1.
    RefSeqi NP_006604.1. NM_006613.3.
    UniGenei Hs.567416.

    3D structure databases

    ProteinModelPortali Q13588.
    SMRi Q13588. Positions 1-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115973. 7 interactions.
    IntActi Q13588. 3 interactions.
    MINTi MINT-1515623.
    STRINGi 9606.ENSP00000284154.

    Polymorphism databases

    DMDMi 3913785.

    Proteomic databases

    MaxQBi Q13588.
    PaxDbi Q13588.
    PRIDEi Q13588.

    Protocols and materials databases

    DNASUi 10750.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000284154 ; ENSP00000284154 ; ENSG00000154016 .
    GeneIDi 10750.
    KEGGi hsa:10750.
    UCSCi uc002guy.3. human.

    Organism-specific databases

    CTDi 10750.
    GeneCardsi GC17M018923.
    H-InvDB HIX0039163.
    HGNCi HGNC:4562. GRAP.
    HPAi HPA046595.
    MIMi 604330. gene.
    neXtProti NX_Q13588.
    PharmGKBi PA28958.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG298780.
    HOGENOMi HOG000251625.
    HOVERGENi HBG005404.
    InParanoidi Q13588.
    OMAi RACFAQA.
    OrthoDBi EOG75F4F6.
    PhylomeDBi Q13588.
    TreeFami TF354288.

    Enzyme and pathway databases

    Reactomei REACT_111040. Signaling by SCF-KIT.
    SignaLinki Q13588.

    Miscellaneous databases

    ChiTaRSi GRAP. human.
    GeneWikii GRAP.
    GenomeRNAii 10750.
    NextBioi 40825.
    PROi Q13588.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13588.
    Bgeei Q13588.
    CleanExi HS_GRAP.
    Genevestigatori Q13588.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR000980. SH2.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 2 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    PS50002. SH3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway."
      Feng G.-S., Ouyang Y.-B., Hu D.-P., Shi Z.-Q., Gentz R., Ni J.
      J. Biol. Chem. 271:12129-12132(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Blood and Brain.
    6. "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation."
      Zhang W., Sloan-Lancaster J., Kitchen J., Trible R.P., Samelson L.E.
      Cell 92:83-92(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LAT.
    7. "Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins."
      Welsh M., Songyang Z., Frantz J.D., Trueb T., Reedquist K.A., Karlsson T., Miyazaki M., Cantley L.C., Band H., Shoelson S.E.
      Oncogene 16:891-901(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHB.

    Entry informationi

    Entry nameiGRAP_HUMAN
    AccessioniPrimary (citable) accession number: Q13588
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3