Q13585 (MTR1L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melatonin-related receptor

Alternative name(s):
G protein-coupled receptor 50
H9

Gene names

Name:

GPR50

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Does not bind melatonin.
Subunit structure	Homodimer, and heterodimer with MTNR1A and MTNR1B. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Hypothalamus and pituitary.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc melatonin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Melatonin-related receptor		PRO_0000069878
Regions
	Topological domain	1 – 30	30	Extracellular Potential
	Transmembrane	31 – 51	21	Helical; Name=1; Potential
	Topological domain	52 – 64	13	Cytoplasmic Potential
	Transmembrane	65 – 85	21	Helical; Name=2; Potential
	Topological domain	86 – 103	18	Extracellular Potential
	Transmembrane	104 – 124	21	Helical; Name=3; Potential
	Topological domain	125 – 143	19	Cytoplasmic Potential
	Transmembrane	144 – 164	21	Helical; Name=4; Potential
	Topological domain	165 – 188	24	Extracellular Potential
	Transmembrane	189 – 209	21	Helical; Name=5; Potential
	Topological domain	210 – 239	30	Cytoplasmic Potential
	Transmembrane	240 – 260	21	Helical; Name=6; Potential
	Topological domain	261 – 273	13	Extracellular Potential
	Transmembrane	274 – 294	21	Helical; Name=7; Potential
	Topological domain	295 – 617	323	Cytoplasmic Potential
	Compositional bias	315 – 612	298	Pro-rich
Amino acid modifications
	Disulfide bond	101 ↔ 178		By similarity
Natural variations
	Natural variant	502 – 505	4	Missing Lower fasting circulating triglyceride levels.		VAR_062254
	Natural variant	532	1	T → A Higher fasting circulating triglyceride levels. Ref.1 Ref.2 Ref.3 Corresponds to variant rs561077 [ dbSNP | Ensembl ].		VAR_062255
	Natural variant	606	1	I → V Higher fasting circulating triglyceride levels. Ref.1 Ref.3 Corresponds to variant rs13440581 [ dbSNP | Ensembl ].		VAR_062256
Experimental info
	Sequence conflict	320	1	S → P in AAC50614. Ref.1
	Sequence conflict	448	1	A → G in AAC50614. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13585 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: A0B73A7ACE1053BB	FASTA	617	67,369
	10 20 30 40 50 60 MGPTLAVPTP YGCIGCKLPQ PEYPPALIIF MFCAMVITIV VDLIGNSMVI LAVTKNKKLR 70 80 90 100 110 120 NSGNIFVVSL SVADMLVAIY PYPLMLHAMS IGGWDLSQLQ CQMVGFITGL SVVGSIFNIV 130 140 150 160 170 180 AIAINRYCYI CHSLQYERIF SVRNTCIYLV ITWIMTVLAV LPNMYIGTIE YDPRTYTCIF 190 200 210 220 230 240 NYLNNPVFTV TIVCIHFVLP LLIVGFCYVR IWTKVLAARD PAGQNPDNQL AEVRNFLTMF 250 260 270 280 290 300 VIFLLFAVCW CPINVLTVLV AVSPKEMAGK IPNWLYLAAY FIAYFNSCLN AVIYGLLNEN 310 320 330 340 350 360 FRREYWTIFH AMRHPIIFFS GLISDIREMQ EARTLARARA HARDQAREQD RAHACPAVEE 370 380 390 400 410 420 TPMNVRNVPL PGDAAAGHPD RASGHPKPHS RSSSAYRKSA STHHKSVFSH SKAASGHLKP 430 440 450 460 470 480 VSGHSKPASG HPKSATVYPK PASVHFKADS VHFKGDSVHF KPDSVHFKPA SSNPKPITGH 490 500 510 520 530 540 HVSAGSHSKS AFSAATSHPK PTTGHIKPAT SHAEPTTADY PKPATTSHPK PTAADNPELS 550 560 570 580 590 600 ASHCPEIPAI AHPVSDDSDL PESASSPAAG PTKPAASQLE SDTIADLPDP TVVTTSTNDY 610 HDVVVIDVED DPDEMAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a melatonin-related receptor from human pituitary." Reppert S.M., Weaver D.R., Ebisawa T., Mahle C.D., Kolakowski L.F. Jr. FEBS Lett. 386:219-224(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS 502-THR--HIS-505 DEL; ALA-532 AND VAL-606. Tissue: Pituitary.
[2]	Kaighin V.A., Martin A.L., Aronstam R.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS 502-THR--HIS-505 DEL AND ALA-532. Tissue: Pituitary.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS 502-THR--HIS-505 DEL; ALA-532 AND VAL-606.
[4]	"The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor function through heterodimerization." Levoye A., Dam J., Ayoub M.A., Guillaume J.L., Couturier C., Delagrange P., Jockers R. EMBO J. 25:3012-3023(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[5]	"Sequence variants in the melatonin-related receptor gene (GPR50) associate with circulating triglyceride and HDL levels." Bhattacharyya S., Luan J., Challis B., Keogh J., Montague C., Brennand J., Morten J., Lowenbeim S., Jenkins S., Farooqi I.S., Wareham N.J., O'Rahilly S. J. Lipid Res. 47:761-766(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS 502THR--HIS505 DEL; ALA-528 AND ILE-606.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52219 mRNA. Translation: AAC50614.1. EU432118 mRNA. Translation: ABY87917.1. BC103696 mRNA. Translation: AAI03697.1. BC105683 mRNA. Translation: AAI05684.1. BC105684 mRNA. Translation: AAI05685.1.
IPI	IPI00299062.
PIR	S70520.
RefSeq	NP_004215.2. NM_004224.3.
UniGene	Hs.567390.
3D structure databases
ProteinModelPortal	Q13585.
SMR	Q13585. Positions 15-314.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2836359.
STRING	9606.ENSP00000218316.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q13585.
Polymorphism databases
DMDM	296439298.
Proteomic databases
PaxDb	Q13585.
PRIDE	Q13585.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000218316; ENSP00000218316; ENSG00000102195.
GeneID	9248.
KEGG	hsa:9248.
UCSC	uc010ntg.2. human.
Organism-specific databases
CTD	9248.
GeneCards	GC0XP150345.
H-InvDB	HIX0056463.
HGNC	HGNC:4506. GPR50.
HPA	HPA049862.
MIM	300207. gene.
neXtProt	NX_Q13585.
PharmGKB	PA28895.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG149107.
HOGENOM	HOG000133094.
HOVERGEN	HBG052529.
InParanoid	Q13585.
KO	K04287.
OMA	PASVHFK.
OrthoDB	EOG4C2H95.
PhylomeDB	Q13585.
Gene expression databases
Bgee	Q13585.
CleanEx	HS_GPR50.
Genevestigator	Q13585.
GermOnline	ENSG00000102195. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR002280. Mel_rcpt_1X. IPR000025. Melatonin_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01151. MELATONIN1XR. PR00857. MELATONINR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9248.
NextBio	34671.
SOURCE	Search...

Entry information

Entry name

MTR1L_HUMAN

Accession

Primary (citable) accession number: Q13585
Secondary accession number(s): Q0VGG3, Q3ZAR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.