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Protein

Melatonin-related receptor

Gene

GPR50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not bind melatonin.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: ProtInc
  2. identical protein binding Source: IntAct
  3. melatonin receptor activity Source: InterPro

GO - Biological processi

  1. cell-cell signaling Source: ProtInc
  2. G-protein coupled receptor signaling pathway Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Melatonin-related receptor
Alternative name(s):
G protein-coupled receptor 50
H9
Gene namesi
Name:GPR50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:4506. GPR50.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030ExtracellularSequence AnalysisAdd
BLAST
Transmembranei31 – 5121Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini52 – 6413CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei65 – 8521Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini86 – 10318ExtracellularSequence AnalysisAdd
BLAST
Transmembranei104 – 12421Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini125 – 14319CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei144 – 16421Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini165 – 18824ExtracellularSequence AnalysisAdd
BLAST
Transmembranei189 – 20921Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini210 – 23930CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei240 – 26021Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini261 – 27313ExtracellularSequence AnalysisAdd
BLAST
Transmembranei274 – 29421Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini295 – 617323CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28895.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 617617Melatonin-related receptorPRO_0000069878Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi101 ↔ 178PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ13585.
PaxDbiQ13585.
PRIDEiQ13585.

PTM databases

PhosphoSiteiQ13585.

Expressioni

Tissue specificityi

Hypothalamus and pituitary.

Gene expression databases

BgeeiQ13585.
CleanExiHS_GPR50.
GenevestigatoriQ13585.

Organism-specific databases

HPAiHPA049862.

Interactioni

Subunit structurei

Homodimer, and heterodimer with MTNR1A and MTNR1B.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8550965,EBI-8550965
MT1AP047313EBI-8550965,EBI-8045030
MT2AP027952EBI-8550965,EBI-996616

Protein-protein interaction databases

BioGridi114674. 8 interactions.
IntActiQ13585. 3 interactions.
MINTiMINT-2836359.
STRINGi9606.ENSP00000218316.

Structurei

3D structure databases

ProteinModelPortaliQ13585.
SMRiQ13585. Positions 15-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi315 – 612298Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG149107.
GeneTreeiENSGT00440000033318.
HOGENOMiHOG000133094.
HOVERGENiHBG052529.
InParanoidiQ13585.
KOiK04287.
OMAiPASVHFK.
OrthoDBiEOG7MD4Q5.
PhylomeDBiQ13585.
TreeFamiTF331693.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002280. Mel_rcpt_1X.
IPR000025. Melatonin_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01151. MELATONIN1XR.
PR00857. MELATONINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPTLAVPTP YGCIGCKLPQ PEYPPALIIF MFCAMVITIV VDLIGNSMVI
60 70 80 90 100
LAVTKNKKLR NSGNIFVVSL SVADMLVAIY PYPLMLHAMS IGGWDLSQLQ
110 120 130 140 150
CQMVGFITGL SVVGSIFNIV AIAINRYCYI CHSLQYERIF SVRNTCIYLV
160 170 180 190 200
ITWIMTVLAV LPNMYIGTIE YDPRTYTCIF NYLNNPVFTV TIVCIHFVLP
210 220 230 240 250
LLIVGFCYVR IWTKVLAARD PAGQNPDNQL AEVRNFLTMF VIFLLFAVCW
260 270 280 290 300
CPINVLTVLV AVSPKEMAGK IPNWLYLAAY FIAYFNSCLN AVIYGLLNEN
310 320 330 340 350
FRREYWTIFH AMRHPIIFFS GLISDIREMQ EARTLARARA HARDQAREQD
360 370 380 390 400
RAHACPAVEE TPMNVRNVPL PGDAAAGHPD RASGHPKPHS RSSSAYRKSA
410 420 430 440 450
STHHKSVFSH SKAASGHLKP VSGHSKPASG HPKSATVYPK PASVHFKADS
460 470 480 490 500
VHFKGDSVHF KPDSVHFKPA SSNPKPITGH HVSAGSHSKS AFSAATSHPK
510 520 530 540 550
PTTGHIKPAT SHAEPTTADY PKPATTSHPK PTAADNPELS ASHCPEIPAI
560 570 580 590 600
AHPVSDDSDL PESASSPAAG PTKPAASQLE SDTIADLPDP TVVTTSTNDY
610
HDVVVIDVED DPDEMAV
Length:617
Mass (Da):67,369
Last modified:May 17, 2010 - v3
Checksum:iA0B73A7ACE1053BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti320 – 3201S → P in AAC50614 (PubMed:8647286).Curated
Sequence conflicti448 – 4481A → G in AAC50614 (PubMed:8647286).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti502 – 5054Missing Lower fasting circulating triglyceride levels. 3 Publications
VAR_062254
Natural varianti532 – 5321T → A Higher fasting circulating triglyceride levels. 3 Publications
Corresponds to variant rs561077 [ dbSNP | Ensembl ].
VAR_062255
Natural varianti606 – 6061I → V Higher fasting circulating triglyceride levels. 2 Publications
Corresponds to variant rs13440581 [ dbSNP | Ensembl ].
VAR_062256

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52219 mRNA. Translation: AAC50614.1.
EU432118 mRNA. Translation: ABY87917.1.
BC103696 mRNA. Translation: AAI03697.1.
BC105683 mRNA. Translation: AAI05684.1.
BC105684 mRNA. Translation: AAI05685.1.
CCDSiCCDS44012.1.
PIRiS70520.
RefSeqiNP_004215.2. NM_004224.3.
UniGeneiHs.567390.

Genome annotation databases

EnsembliENST00000218316; ENSP00000218316; ENSG00000102195.
GeneIDi9248.
KEGGihsa:9248.
UCSCiuc010ntg.2. human.

Polymorphism databases

DMDMi296439298.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52219 mRNA. Translation: AAC50614.1.
EU432118 mRNA. Translation: ABY87917.1.
BC103696 mRNA. Translation: AAI03697.1.
BC105683 mRNA. Translation: AAI05684.1.
BC105684 mRNA. Translation: AAI05685.1.
CCDSiCCDS44012.1.
PIRiS70520.
RefSeqiNP_004215.2. NM_004224.3.
UniGeneiHs.567390.

3D structure databases

ProteinModelPortaliQ13585.
SMRiQ13585. Positions 15-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114674. 8 interactions.
IntActiQ13585. 3 interactions.
MINTiMINT-2836359.
STRINGi9606.ENSP00000218316.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ13585.

Polymorphism databases

DMDMi296439298.

Proteomic databases

MaxQBiQ13585.
PaxDbiQ13585.
PRIDEiQ13585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000218316; ENSP00000218316; ENSG00000102195.
GeneIDi9248.
KEGGihsa:9248.
UCSCiuc010ntg.2. human.

Organism-specific databases

CTDi9248.
GeneCardsiGC0XP150345.
H-InvDBHIX0056463.
HGNCiHGNC:4506. GPR50.
HPAiHPA049862.
MIMi300207. gene.
neXtProtiNX_Q13585.
PharmGKBiPA28895.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG149107.
GeneTreeiENSGT00440000033318.
HOGENOMiHOG000133094.
HOVERGENiHBG052529.
InParanoidiQ13585.
KOiK04287.
OMAiPASVHFK.
OrthoDBiEOG7MD4Q5.
PhylomeDBiQ13585.
TreeFamiTF331693.

Miscellaneous databases

GeneWikiiGPR50.
GenomeRNAii9248.
NextBioi34671.
PROiQ13585.
SOURCEiSearch...

Gene expression databases

BgeeiQ13585.
CleanExiHS_GPR50.
GenevestigatoriQ13585.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002280. Mel_rcpt_1X.
IPR000025. Melatonin_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01151. MELATONIN1XR.
PR00857. MELATONINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a melatonin-related receptor from human pituitary."
    Reppert S.M., Weaver D.R., Ebisawa T., Mahle C.D., Kolakowski L.F. Jr.
    FEBS Lett. 386:219-224(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS 502-THR--HIS-505 DEL; ALA-532 AND VAL-606.
    Tissue: Pituitary.
  2. Kaighin V.A., Martin A.L., Aronstam R.S.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS 502-THR--HIS-505 DEL AND ALA-532.
    Tissue: Pituitary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS 502-THR--HIS-505 DEL; ALA-532 AND VAL-606.
  4. "The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor function through heterodimerization."
    Levoye A., Dam J., Ayoub M.A., Guillaume J.L., Couturier C., Delagrange P., Jockers R.
    EMBO J. 25:3012-3023(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "Sequence variants in the melatonin-related receptor gene (GPR50) associate with circulating triglyceride and HDL levels."
    Bhattacharyya S., Luan J., Challis B., Keogh J., Montague C., Brennand J., Morten J., Lowenbeim S., Jenkins S., Farooqi I.S., Wareham N.J., O'Rahilly S.
    J. Lipid Res. 47:761-766(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS 502THR--HIS505 DEL; ALA-528 AND ILE-606.

Entry informationi

Entry nameiMTR1L_HUMAN
AccessioniPrimary (citable) accession number: Q13585
Secondary accession number(s): Q0VGG3, Q3ZAR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: May 17, 2010
Last modified: February 3, 2015
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.