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Reviewed, UniProtKB/Swiss-Prot Q13574 (DGKZ_HUMAN)

Last modified November 3, 2009. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Diacylglycerol kinase zeta
      Short name=DAG kinase zeta
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase zeta
      Short name=DGK-zeta
Gene names
Name: DGKZ
Synonyms: DAGK6
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1117 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. Ref.6

Catalytic activity

ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Subunit structure

Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Isoform 2 forms a signaling complex with RASGRP1 and HRAS. Ref.5 Ref.7

Subcellular location

Cytoplasm. Nucleus. Cell membrane. Ref.6 Ref.4

Tissue specificity

Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.

Post-translational modification

Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 2 ANK repeats.

Contains 1 DAGKc domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Sequence caution

The sequence AAB60859.1 differs from that shown. Reason: Frameshift at positions 166 and 167.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Membrane
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainANK repeat
Phorbol-ester binding
Repeat
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processG1 DNA damage checkpoint

Inferred from genetic interaction. Source: UniProtKB

activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway

Inferred from electronic annotation. Source: InterPro

intracellular signaling cascade

Inferred from electronic annotation. Source: InterPro

negative regulation of mitotic cell cycle

Inferred from genetic interaction. Source: UniProtKB

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding Ref.1

Traceable author statement. Source: ProtInc

diacylglycerol binding

Inferred from electronic annotation. Source: UniProtKB-KW

diacylglycerol kinase activity

Inferred from direct assay. Source: UniProtKB

lipid kinase activity

Inferred from direct assay. Source: UniProtKB

protein C-terminus binding

Inferred from direct assay. Source: UniProtKB

protein binding

Inferred from physical interaction. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13574-1)

Also known as: Long; zeta2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Minor isoform.
Isoform 2 (identifier: Q13574-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: METFFRRHFR...GPQAWSALLA → MEPRDGSPEA...FPGLRLFGHR
Note: Major isoform.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11171117Diacylglycerol kinase zeta
PRO_0000218468

Regions

Domain480 – 614135DAGKc
Repeat1011 – 104131ANK 1
Repeat1046 – 107530ANK 2
Zinc finger287 – 34155Phorbol-ester/DAG-type 1
Zinc finger361 – 41959Phorbol-ester/DAG-type 2
Region448 – 46215MARCKS homology
Region467 – 605139Mediates interaction with RASGRP1
Compositional bias256 – 2616Poly-Pro
Compositional bias448 – 4514Poly-Lys
Compositional bias560 – 5634Poly-Pro

Amino acid modifications

Modified residue6861Phosphoserine Ref.8

Natural variations

Alternative sequence1 – 243243METFF…SALLA → MEPRDGSPEARSSDSESASA SSSGSERDAGPEPDKAPRRL NKRRFPGLRLFGHR in isoform 2.
VSP_001268
Natural variant211Q → R: dbSNP rs1317826. Ref.2
VAR_047371

Experimental info

Mutagenesis1115 – 11162TA → NS: Loss of interaction with SNTG1. Ref.5
Sequence conflict1591L → V in AAB60859. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (zeta2) [UniParc].

Last modified November 25, 2008. Version 3.
Checksum: F3A7C2382ECF549B

FASTA1,117124,128
        10         20         30         40         50         60 
METFFRRHFR GKVPGPGEGQ QRPSSVGLPT GKARRRSPAG QASSSLAQRR RSSAQLQGCL 

        70         80         90        100        110        120 
LSCGVRAQGS SRRRSSTVPP SCNPRFIVDK VLTPQPTTVG AQLLGAPLLL TGLVGMNEEE 

       130        140        150        160        170        180 
GVQEDVVAEA SSAIQPGTKT PGPPPPRGAQ PLLPLPRYLR RASSHLLPAD AVYDHALWGL 

       190        200        210        220        230        240 
HGYYRRLSQR RPSGQHPGPG GRRASGTTAG TMLPTRVRPL SRRRQVALRR KAAGPQAWSA 

       250        260        270        280        290        300 
LLAKAITKSG LQHLAPPPPT PGAPCSESER QIRSTVDWSE SATYGEHIWF ETNVSGDFCY 

       310        320        330        340        350        360 
VGEQYCVARM LKSVSRRKCA ACKIVVHTPC IEQLEKINFR CKPSFRESGS RNVREPTFVR 

       370        380        390        400        410        420 
HHWVHRRRQD GKCRHCGKGF QQKFTFHSKE IVAISCSWCK QAYHSKVSCF MLQQIEEPCS 

       430        440        450        460        470        480 
LGVHAAVVIP PTWILRARRP QNTLKASKKK KRASFKRKSS KKGPEEGRWR PFIIRPTPSP 

       490        500        510        520        530        540 
LMKPLLVFVN PKSGGNQGAK IIQSFLWYLN PRQVFDLSQG GPKEALEMYR KVHNLRILAC 

       550        560        570        580        590        600 
GGDGTVGWIL STLDQLRLKP PPPVAILPLG TGNDLARTLN WGGGYTDEPV SKILSHVEEG 

       610        620        630        640        650        660 
NVVQLDRWDL HAEPNPEAGP EDRDEGATDR LPLDVFNNYF SLGFDAHVTL EFHESREANP 

       670        680        690        700        710        720 
EKFNSRFRNK MFYAGTAFSD FLMGSSKDLA KHIRVVCDGM DLTPKIQDLK PQCVVFLNIP 

       730        740        750        760        770        780 
RYCAGTMPWG HPGEHHDFEP QRHDDGYLEV IGFTMTSLAA LQVGGHGERL TQCREVVLTT 

       790        800        810        820        830        840 
SKAIPVQVDG EPCKLAASRI RIALRNQATM VQKAKRRSAA PLHSDQQPVP EQLRIQVSRV 

       850        860        870        880        890        900 
SMHDYEALHY DKEQLKEASV PLGTVVVPGD SDLELCRAHI ERLQQEPDGA GAKSPTCQKL 

       910        920        930        940        950        960 
SPKWCFLDAT TASRFYRIDR AQEHLNYVTE IAQDEIYILD PELLGASARP DLPTPTSPLP 

       970        980        990       1000       1010       1020 
TSPCSPTPRS LQGDAAPPQG EELIEAAKRN DFCKLQELHR AGGDLMHRDE QSRTLLHHAV 

      1030       1040       1050       1060       1070       1080 
STGSKDVVRY LLDHAPPEIL DAVEENGETC LHQAAALGQR TICHYIVEAG ASLMKTDQQG 

      1090       1100       1110 
DTPRQRAEKA QDTELAAYLE NRQHYQMIQR EDQETAV 

« Hide

Isoform 2 (Short).

Checksum: 7936A979CB3D35AA
Show »

FASTA928103,981

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel human diacylglycerol kinase zeta."
Bunting M., Tang W., Zimmerman G.A., McIntyre T.M., Prescott S.M.
J. Biol. Chem. 271:10230-10236(1996) [PubMed: 8626588] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Endothelial cell.
[2]"Alternative splicing of the human diacylglycerol kinase zeta gene in muscle."
Ding L., Bunting M., Topham M.K., McIntyre T.M., Zimmerman G.A., Prescott S.M.
Proc. Natl. Acad. Sci. U.S.A. 94:5519-5524(1997) [PubMed: 9159104] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-21.
Tissue: Skeletal muscle.
[3]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed: 16554811] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta."
Topham M.K., Bunting M., Zimmerman G.A., McIntyre T.M., Blackshear P.J., Prescott S.M.
Nature 394:697-700(1998) [PubMed: 9716136] [Abstract]
Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
[5]"Interaction of gamma 1-syntrophin with diacylglycerol kinase-zeta. Regulation of nuclear localization by PDZ interactions."
Hogan A., Shepherd L., Chabot J., Quenneville S., Prescott S.M., Topham M.K., Gee S.H.
J. Biol. Chem. 276:26526-26533(2001) [PubMed: 11352924] [Abstract]
Cited for: INTERACTION WITH SNTG1, MUTAGENESIS OF 1115-THR-ALA-1116.
[6]"Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism."
Topham M.K., Prescott S.M.
J. Cell Biol. 152:1135-1143(2001) [PubMed: 11257115] [Abstract]
Cited for: FUNCTION AS A RASGRP1 REGULATOR, IDENTIFICATION IN A COMPLEX WITH RASGRP1 AND HRAS, SUBCELLULAR LOCATION.
[7]"Proteomics identification of sorting nexin 27 as a diacylglycerol kinase zeta-associated protein: new diacylglycerol kinase roles in endocytic recycling."
Rincon E., Santos T., Avila-Flores A., Albar J.P., Lalioti V., Lei C., Hong W., Merida I.
Mol. Cell. Proteomics 6:1073-1087(2007) [PubMed: 17351151] [Abstract]
Cited for: INTERACTION WITH SNX27.
[8]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-686, MASS SPECTROMETRY.
Tissue: Epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

U51477 mRNA. Translation: AAC50478.1.
U94905 mRNA. Translation: AAB60859.1. Frameshift.
AC116021 Genomic DNA. No translation available.
IPIIPI00013835.
IPI00300125.
RefSeqNP_001099010.1.
NP_003637.2.
NP_963291.1.
UniGeneHs.502461

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ13574. 8 interactions.
STRINGQ13574.

PTM databases

PhosphoSiteQ13574.

Proteomic databases

PRIDEQ13574.

Genome annotation databases

EnsemblENST00000318201; ENSP00000320340; ENSG00000149091; Homo sapiens. [Genome view]
ENST00000343674; ENSP00000343065; ENSG00000149091; Homo sapiens. [Genome view]
ENST00000395574; ENSP00000378941; ENSG00000149091; Homo sapiens. [Genome view]
ENST00000421244; ENSP00000391021; ENSG00000149091; Homo sapiens. [Genome view]
ENST00000454345; ENSP00000412178; ENSG00000149091; Homo sapiens. [Genome view]
ENST00000456247; ENSP00000395684; ENSG00000149091; Homo sapiens. [Genome view]
GeneID8525.
KEGGhsa:8525.
UCSCuc001ncm.2. human.
uc001ncn.1. human.

Organism-specific databases

CTD8525.
GeneCardsGC11P046311.
H-InvDBHIX0009600.
HGNCHGNC:2857. DGKZ.
MIM601441. gene.
PharmGKBPA27318.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ13574.
HOVERGENQ13574.
OMAGDLMHRD.

Enzyme and pathway databases

BRENDA2.7.1.107. 247.

Gene expression databases

ArrayExpressQ13574.
BgeeQ13574.
CleanExHS_DGKZ.
GenevestigatorQ13574.
GermOnlineENSG00000149091. Homo sapiens.

Family and domain databases

InterProIPR002110. ANK.
IPR000756. Diacylglycerol_kinase_access.
IPR001206. Diacylglycerol_kinase_cat.
IPR002219. Prot_Kinase_C-like_PE/DAG_bd.
[Graphical view]
Gene3DG3DSA:1.25.40.20. ANK. 1 hit.
PfamPF00023. Ank. 2 hits.
PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
ProDomPD002939. DAGKa. 1 hit.
PD005043. DAGKc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. False negative.
PS50081. ZF_DAG_PE_2. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio31916.
SOURCESearch...

Entry information

Entry nameDGKZ_HUMAN
AccessionPrimary (citable) accession number: Q13574
Secondary accession number(s): O00542
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 25, 2008
Last modified: November 3, 2009
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents