ID   DCTN2_HUMAN             Reviewed;         401 AA.
AC   Q13561; Q86YN2; Q9BW17;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   07-JUL-2009, entry version 86.
DE   RecName: Full=Dynactin subunit 2;
DE   AltName: Full=Dynactin complex 50 kDa subunit;
DE            Short=DCTN-50;
DE   AltName: Full=50 kDa dynein-associated polypeptide;
DE   AltName: Full=p50 dynamitin;
GN   Name=DCTN2; Synonyms=DCTN50;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=96178072; PubMed=8647893; DOI=10.1083/jcb.132.4.617;
RA   Echeverri C.J., Paschal B.M., Vaughan K.T., Vallee R.B.;
RT   "Molecular characterization of the 50-kD subunit of dynactin reveals
RT   function for the complex in chromosome alignment and spindle
RT   organization during mitosis.";
RL   J. Cell Biol. 132:617-633(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta, Skin, and Uterus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-385.
RA   Aumais J.P., Yu-Lee L.-Y.;
RT   "Human 50 kD dynactin subunit, p50 dynamitin, isolated from HeLa
RT   cells.";
RL   Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 2-14.
RC   TISSUE=Platelet;
RX   MEDLINE=22608298; PubMed=12665801; DOI=10.1038/nbt810;
RA   Gevaert K., Goethals M., Martens L., Van Damme J., Staes A.,
RA   Thomas G.R., Vandekerckhove J.;
RT   "Exploring proteomes and analyzing protein processing by mass
RT   spectrometric identification of sorted N-terminal peptides.";
RL   Nat. Biotechnol. 21:566-569(2003).
RN   [5]
RP   PROTEIN SEQUENCE OF 2-14, CLEAVAGE OF INITIATOR METHIONINE,
RP   ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
RC   TISSUE=Embryonic kidney;
RA   Bienvenut W.V., Ramsay A., Leung H.Y.;
RL   Submitted (FEB-2009) to UniProtKB.
RN   [6]
RP   PROTEIN SEQUENCE OF 6-14; 79-96; 106-119; 157-185; 231-282 AND
RP   366-395, AND MASS SPECTROMETRY.
RC   TISSUE=Fetal brain cortex;
RA   Lubec G., Chen W.-Q., Sun Y.;
RL   Submitted (DEC-2008) to UniProtKB.
RN   [7]
RP   INTERACTION WITH MAPRE1.
RX   PubMed=10226031; DOI=10.1016/S0960-9822(99)80190-0;
RA   Berrueta L., Tirnauer J.S., Schuyler S.C., Pellman D., Bierer B.E.;
RT   "The APC-associated protein EB1 associates with components of the
RT   dynactin complex and cytoplasmic dynein intermediate chain.";
RL   Curr. Biol. 9:425-428(1999).
RN   [8]
RP   IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RA   Colinge J., Superti-Furga G., Bennett K.L.;
RL   Submitted (OCT-2008) to UniProtKB.
CC   -!- FUNCTION: Modulates cytoplasmic dynein binding to an organelle,
CC       and plays a role in prometaphase chromosome alignment and spindle
CC       organization during mitosis. May play a role in synapse formation
CC       during brain development.
CC   -!- SUBUNIT: Subunit of dynactin, a multiprotein complex associated
CC       with dynein. Interacts with BICD2 (By similarity). Interacts with
CC       MAPRE1.
CC   -!- INTERACTION:
CC       Q9NRI5:DISC1; NbExp=2; IntAct=EBI-715074, EBI-529989;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Membrane;
CC       Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the dynactin subunit 2 family.
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DR   EMBL; U50733; AAC50423.1; -; mRNA.
DR   EMBL; BC000718; AAH00718.1; -; mRNA.
DR   EMBL; BC009468; AAH09468.1; -; mRNA.
DR   EMBL; BC014083; AAH14083.1; -; mRNA.
DR   EMBL; AY189155; AAO34395.1; -; mRNA.
DR   IPI; IPI00220503; -.
DR   RefSeq; NP_006391.1; -.
DR   UniGene; Hs.151219; -.
DR   UniGene; Hs.289123; -.
DR   IntAct; Q13561; 5.
DR   PhosphoSite; Q13561; -.
DR   REPRODUCTION-2DPAGE; IPI00220503; -.
DR   PRIDE; Q13561; -.
DR   Ensembl; ENSG00000175203; Homo sapiens.
DR   GeneID; 10540; -.
DR   KEGG; hsa:10540; -.
DR   UCSC; uc001som.1; human.
DR   GeneCards; GC12M056210; -.
DR   HGNC; HGNC:2712; DCTN2.
DR   MIM; 607376; gene.
DR   PharmGKB; PA27181; -.
DR   HOVERGEN; Q13561; -.
DR   Reactome; REACT_152; Cell Cycle, Mitotic.
DR   NextBio; 39989; -.
DR   PMAP-CutDB; Q13561; -.
DR   Bgee; Q13561; -.
DR   CleanEx; HS_DCTN2; -.
DR   GermOnline; ENSG00000175203; Homo sapiens.
DR   GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR   GO; GO:0005869; C:dynactin complex; IDA:UniProtKB.
DR   GO; GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0003774; F:motor activity; IEA:UniProtKB-KW.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0008283; P:cell proliferation; IMP:UniProtKB.
DR   GO; GO:0007017; P:microtubule-based process; IEA:InterPro.
DR   GO; GO:0007067; P:mitosis; IMP:UniProtKB.
DR   InterPro; IPR006996; Dynamitin.
DR   Pfam; PF04912; Dynamitin; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton;
KW   Direct protein sequencing; Dynein; Membrane; Microtubule;
KW   Phosphoprotein.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    401       Dynactin subunit 2.
FT                                /FTId=PRO_0000079821.
FT   COILED       99    132       Potential.
FT   COILED      214    244       Potential.
FT   COILED      379    399       Potential.
FT   MOD_RES       2      2       N-acetylalanine.
FT   MOD_RES      83     83       Phosphoserine (By similarity).
FT   MOD_RES      86     86       Phosphotyrosine (By similarity).
FT   CONFLICT     35     35       A -> AFAQEL (in Ref. 1; AAC50423).
FT   CONFLICT     36     36       E -> ELE (in Ref. 3).
FT   CONFLICT    382    385       LATV -> PGHS (in Ref. 3).
SQ   SEQUENCE   401 AA;  44231 MW;  C2FF01A9739337B2 CRC64;
     MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELTS TSVEHIIVNP NAAYDKFKDK
     RVGTKGLDFS DRIGKTKRTG YESGEYEMLG EGLGVKETPQ QKYQRLLHEV QELTTEVEKI
     KTTVKESATE EKLTPVLLAK QLAALKQQLV ASHLEKLLGP DAAINLTDPD GALAKRLLLQ
     LEATKNSKGG SGGKTTGTPP DSSLVTYELH SRPEQDKFSQ AAKVAELEKR LTELETAVRC
     DQDAQNPLSA GLQGACLMET VELLQAKVSA LDLAVLDQVE ARLQSVLGKV NEIAKHKASV
     EDADTQSKVH QLYETIQRWS PIASTLPELV QRLVTIKQLH EQAMQFGQLL THLDTTQQMI
     ANSLKDNTTL LTQVQTTMRE NLATVEGNFA SIDERMKKLG K
//