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Protein

Dynactin subunit 2

Gene

DCTN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

GO - Molecular functioni

  • motor activity Source: UniProtKB-KW
  • spectrin binding Source: MGI

GO - Biological processi

  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  • cell proliferation Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: Reactome
  • G2/M transition of mitotic cell cycle Source: Reactome
  • melanosome transport Source: Ensembl
  • microtubule-based process Source: InterPro
  • mitotic nuclear division Source: UniProtKB
  • mitotic spindle organization Source: GO_Central
  • protein localization to centrosome Source: SYSCILIA_CCNET
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175203-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-HSA-8854518. AURKA Activation by TPX2.
SignaLinkiQ13561.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 2
Alternative name(s):
50 kDa dynein-associated polypeptide
Dynactin complex 50 kDa subunit
Short name:
DCTN-50
p50 dynamitin
Gene namesi
Name:DCTN2
Synonyms:DCTN50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:2712. DCTN2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • dynactin complex Source: UniProtKB
  • dynein complex Source: UniProtKB-KW
  • extracellular exosome Source: UniProtKB
  • growth cone Source: Ensembl
  • kinetochore Source: UniProtKB
  • membrane Source: UniProtKB-SubCell
  • microtubule Source: UniProtKB-KW
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi10540.
OpenTargetsiENSG00000175203.
PharmGKBiPA27181.

Polymorphism and mutation databases

BioMutaiDCTN2.
DMDMi22096346.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00000798212 – 401Dynactin subunit 2Add BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei6PhosphotyrosineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei86PhosphotyrosineBy similarity1
Modified residuei134PhosphothreonineCombined sources1
Modified residuei198PhosphothreonineCombined sources1
Modified residuei320PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13561.
MaxQBiQ13561.
PaxDbiQ13561.
PeptideAtlasiQ13561.
PRIDEiQ13561.

2D gel databases

REPRODUCTION-2DPAGEIPI00220503.

PTM databases

iPTMnetiQ13561.
PhosphoSitePlusiQ13561.

Miscellaneous databases

PMAP-CutDBQ13561.

Expressioni

Gene expression databases

BgeeiENSG00000175203.
CleanExiHS_DCTN2.
ExpressionAtlasiQ13561. baseline and differential.
GenevisibleiQ13561. HS.

Organism-specific databases

HPAiHPA039715.
HPA040040.

Interactioni

Subunit structurei

Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 and CEP135 (By similarity). Interacts with DYNAP, ECM29 and MAPRE1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-715074,EBI-715074
BLOC1S6Q9UL453EBI-715074,EBI-465781
CCDC172P0C7W63EBI-715074,EBI-2548868
CEP44Q9C0F13EBI-715074,EBI-744115
DISC1Q9NRI53EBI-715074,EBI-529989
GADD45AP245224EBI-715074,EBI-448167
HAUS1Q96CS23EBI-715074,EBI-2514791
HTTP428586EBI-715074,EBI-466029
KIFC3Q9BVG83EBI-715074,EBI-2125614
MAPRE3Q9UPY83EBI-715074,EBI-726739

GO - Molecular functioni

  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi115794. 110 interactors.
DIPiDIP-37545N.
IntActiQ13561. 79 interactors.
MINTiMINT-206219.
STRINGi9606.ENSP00000408910.

Structurei

3D structure databases

ProteinModelPortaliQ13561.
SMRiQ13561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili99 – 132Sequence analysisAdd BLAST34
Coiled coili214 – 244Sequence analysisAdd BLAST31
Coiled coili379 – 399Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the dynactin subunit 2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3958. Eukaryota.
ENOG410XSZK. LUCA.
GeneTreeiENSGT00390000003427.
HOVERGENiHBG051323.
InParanoidiQ13561.
KOiK10424.
OMAiKYQRLVN.
PhylomeDBiQ13561.
TreeFamiTF105247.

Family and domain databases

InterProiIPR028133. Dynamitin.
[Graphical view]
PANTHERiPTHR15346:SF0. PTHR15346:SF0. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13561-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELTS TSVEHIIVNP
60 70 80 90 100
NAAYDKFKDK RVGTKGLDFS DRIGKTKRTG YESGEYEMLG EGLGVKETPQ
110 120 130 140 150
QKYQRLLHEV QELTTEVEKI KTTVKESATE EKLTPVLLAK QLAALKQQLV
160 170 180 190 200
ASHLEKLLGP DAAINLTDPD GALAKRLLLQ LEATKNSKGG SGGKTTGTPP
210 220 230 240 250
DSSLVTYELH SRPEQDKFSQ AAKVAELEKR LTELETAVRC DQDAQNPLSA
260 270 280 290 300
GLQGACLMET VELLQAKVSA LDLAVLDQVE ARLQSVLGKV NEIAKHKASV
310 320 330 340 350
EDADTQSKVH QLYETIQRWS PIASTLPELV QRLVTIKQLH EQAMQFGQLL
360 370 380 390 400
THLDTTQQMI ANSLKDNTTL LTQVQTTMRE NLATVEGNFA SIDERMKKLG

K
Length:401
Mass (Da):44,231
Last modified:January 23, 2007 - v4
Checksum:iC2FF01A9739337B2
GO
Isoform 2 (identifier: Q13561-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-35: A → AFAQEL

Show »
Length:406
Mass (Da):44,820
Checksum:i1166997A6D07479D
GO
Isoform 3 (identifier: Q13561-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-35: A → AEL

Show »
Length:403
Mass (Da):44,473
Checksum:i864FD68AF5433E22
GO

Sequence cautioni

The sequence AAO34395 differs from that shown. Reason: Frameshift at position 381.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04048535A → AFAQEL in isoform 2. 2 Publications1
Alternative sequenceiVSP_04048635A → AEL in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50733 mRNA. Translation: AAC50423.1.
AK314705 mRNA. Translation: BAG37252.1.
AC022366 Genomic DNA. No translation available.
AC022506 Genomic DNA. No translation available.
BC000718 mRNA. Translation: AAH00718.1.
BC009468 mRNA. Translation: AAH09468.1.
BC014083 mRNA. Translation: AAH14083.1.
AY189155 mRNA. Translation: AAO34395.1. Frameshift.
CCDSiCCDS44930.1. [Q13561-2]
CCDS58245.1. [Q13561-1]
CCDS73489.1. [Q13561-3]
RefSeqiNP_001248341.1. NM_001261412.1. [Q13561-3]
NP_001248342.1. NM_001261413.1. [Q13561-1]
NP_006391.1. NM_006400.4. [Q13561-2]
UniGeneiHs.289123.

Genome annotation databases

EnsembliENST00000434715; ENSP00000408910; ENSG00000175203. [Q13561-2]
ENST00000543672; ENSP00000439376; ENSG00000175203. [Q13561-3]
ENST00000548249; ENSP00000447824; ENSG00000175203. [Q13561-1]
GeneIDi10540.
KEGGihsa:10540.
UCSCiuc001som.3. human. [Q13561-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50733 mRNA. Translation: AAC50423.1.
AK314705 mRNA. Translation: BAG37252.1.
AC022366 Genomic DNA. No translation available.
AC022506 Genomic DNA. No translation available.
BC000718 mRNA. Translation: AAH00718.1.
BC009468 mRNA. Translation: AAH09468.1.
BC014083 mRNA. Translation: AAH14083.1.
AY189155 mRNA. Translation: AAO34395.1. Frameshift.
CCDSiCCDS44930.1. [Q13561-2]
CCDS58245.1. [Q13561-1]
CCDS73489.1. [Q13561-3]
RefSeqiNP_001248341.1. NM_001261412.1. [Q13561-3]
NP_001248342.1. NM_001261413.1. [Q13561-1]
NP_006391.1. NM_006400.4. [Q13561-2]
UniGeneiHs.289123.

3D structure databases

ProteinModelPortaliQ13561.
SMRiQ13561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115794. 110 interactors.
DIPiDIP-37545N.
IntActiQ13561. 79 interactors.
MINTiMINT-206219.
STRINGi9606.ENSP00000408910.

PTM databases

iPTMnetiQ13561.
PhosphoSitePlusiQ13561.

Polymorphism and mutation databases

BioMutaiDCTN2.
DMDMi22096346.

2D gel databases

REPRODUCTION-2DPAGEIPI00220503.

Proteomic databases

EPDiQ13561.
MaxQBiQ13561.
PaxDbiQ13561.
PeptideAtlasiQ13561.
PRIDEiQ13561.

Protocols and materials databases

DNASUi10540.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000434715; ENSP00000408910; ENSG00000175203. [Q13561-2]
ENST00000543672; ENSP00000439376; ENSG00000175203. [Q13561-3]
ENST00000548249; ENSP00000447824; ENSG00000175203. [Q13561-1]
GeneIDi10540.
KEGGihsa:10540.
UCSCiuc001som.3. human. [Q13561-1]

Organism-specific databases

CTDi10540.
DisGeNETi10540.
GeneCardsiDCTN2.
H-InvDBHIX0010763.
HGNCiHGNC:2712. DCTN2.
HPAiHPA039715.
HPA040040.
MIMi607376. gene.
neXtProtiNX_Q13561.
OpenTargetsiENSG00000175203.
PharmGKBiPA27181.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3958. Eukaryota.
ENOG410XSZK. LUCA.
GeneTreeiENSGT00390000003427.
HOVERGENiHBG051323.
InParanoidiQ13561.
KOiK10424.
OMAiKYQRLVN.
PhylomeDBiQ13561.
TreeFamiTF105247.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175203-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-HSA-8854518. AURKA Activation by TPX2.
SignaLinkiQ13561.

Miscellaneous databases

ChiTaRSiDCTN2. human.
GeneWikiiDCTN2.
GenomeRNAii10540.
PMAP-CutDBQ13561.
PROiQ13561.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175203.
CleanExiHS_DCTN2.
ExpressionAtlasiQ13561. baseline and differential.
GenevisibleiQ13561. HS.

Family and domain databases

InterProiIPR028133. Dynamitin.
[Graphical view]
PANTHERiPTHR15346:SF0. PTHR15346:SF0. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCTN2_HUMAN
AccessioniPrimary (citable) accession number: Q13561
Secondary accession number(s): B2RBK5, Q86YN2, Q9BW17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 167 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.