Reviewed,
UniProtKB/Swiss-Prot Q13561 (DCTN2_HUMAN)
Last modified
July 7, 2009.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dynactin subunit 2 Alternative name(s): Dynactin complex 50 kDa subunit Short name=DCTN-50 50 kDa dynein-associated polypeptide p50 dynamitin | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 401 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. May play a role in synapse formation during brain development. |
| Subunit structure | Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 By similarity. Interacts with MAPRE1. |
| Subcellular location | Cytoplasm › cytoskeleton. Membrane; Peripheral membrane protein. |
| Sequence similarities | Belongs to the dynactin subunit 2 family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.4 Ref.5 | ||||||
| Chain | 2 – 401 | 400 | Dynactin subunit 2 | PRO_0000079821 | |||||
Regions | |||||||||
| Coiled coil | 99 – 132 | 34 | Potential | ||||||
| Coiled coil | 214 – 244 | 31 | Potential | ||||||
| Coiled coil | 379 – 399 | 21 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.5 | ||||||
| Modified residue | 83 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 86 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 35 | 1 | A → AFAQEL in AAC50423. Ref.1 | ||||||
| Sequence conflict | 36 | 1 | E → ELE Ref.3 | ||||||
| Sequence conflict | 382 – 385 | 4 | LATV → PGHS Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis." Echeverri C.J., Paschal B.M., Vaughan K.T., Vallee R.B. J. Cell Biol. 132:617-633(1996) [PubMed: 8647893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta, Skin and Uterus. |
| [3] | "Human 50 kD dynactin subunit, p50 dynamitin, isolated from HeLa cells." Aumais J.P., Yu-Lee L.-Y. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-385. |
| [4] | "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Tissue: Platelet. |
| [5] | Bienvenut W.V., Ramsay A., Leung H.Y. Submitted (FEB-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-14, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [6] | Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 6-14; 79-96; 106-119; 157-185; 231-282 AND 366-395, MASS SPECTROMETRY. Tissue: Fetal brain cortex. |
| [7] | "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain." Berrueta L., Tirnauer J.S., Schuyler S.C., Pellman D., Bierer B.E. Curr. Biol. 9:425-428(1999) [PubMed: 10226031] [Abstract] Cited for: INTERACTION WITH MAPRE1. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U50733 mRNA. Translation: AAC50423.1. BC000718 mRNA. Translation: AAH00718.1. BC009468 mRNA. Translation: AAH09468.1. BC014083 mRNA. Translation: AAH14083.1. AY189155 mRNA. Translation: AAO34395.1. | |
| IPI | IPI00220503. |
| RefSeq | NP_006391.1. |
| UniGene | Hs.151219 Hs.289123 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q13561. 5 interactions. |
PTM databases | |
| PhosphoSite | Q13561. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | IPI00220503. |
Proteomic databases | |
| PRIDE | Q13561. |
Genome annotation databases | |
| Ensembl | ENSG00000175203. Homo sapiens. [Contig view] |
| GeneID | 10540. |
| KEGG | hsa:10540. |
| UCSC | uc001som.1. human. |
Organism-specific databases | |
| GeneCards | GC12M056210. |
| HGNC | HGNC:2712. DCTN2. |
| MIM | 607376. gene. |
| PharmGKB | PA27181. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | Q13561. |
Enzyme and pathway databases | |
| Reactome | REACT_152. Cell Cycle, Mitotic. |
Gene expression databases | |
| Bgee | Q13561. |
| CleanEx | HS_DCTN2. |
| GermOnline | ENSG00000175203. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR006996. Dynamitin. [Graphical view] |
| Pfam | PF04912. Dynamitin. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 39989. |
| PMAP-CutDB | Q13561. |
| SOURCE | Search... |
Entry information
| Entry name | DCTN2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13561 Secondary accession number(s): Q86YN2, Q9BW17 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 12 Human chromosome 12: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


