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Protein

Serine/threonine-protein kinase PRP4 homolog

Gene

PRPF4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei717ATPPROSITE-ProRule annotation1
Active sitei815Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi693 – 701ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • protein kinase activity Source: ProtInc
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • protein phosphorylation Source: ProtInc
  • RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS03613-MONOMER.
BRENDAi2.7.11.1. 2681.
SignaLinkiQ13523.
SIGNORiQ13523.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PRP4 homolog (EC:2.7.11.1)
Alternative name(s):
PRP4 kinase
PRP4 pre-mRNA-processing factor 4 homolog
Gene namesi
Name:PRPF4B
Synonyms:KIAA0536, PRP4, PRP4H, PRP4K
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:17346. PRPF4B.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • chromosome Source: Ensembl
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8899.
OpenTargetsiENSG00000112739.
PharmGKBiPA38447.

Chemistry databases

ChEMBLiCHEMBL1908382.
GuidetoPHARMACOLOGYi2177.

Polymorphism and mutation databases

BioMutaiPRPF4B.
DMDMi317373526.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000865862 – 1007Serine/threonine-protein kinase PRP4 homologAdd BLAST1006

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei8PhosphoserineBy similarity1
Modified residuei20PhosphoserineCombined sources1
Modified residuei23PhosphoserineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei87PhosphoserineCombined sources1
Modified residuei93PhosphoserineCombined sources1
Modified residuei99N6-acetyllysineBy similarity1
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei131PhosphoserineCombined sources1
Modified residuei140PhosphotyrosineCombined sources1
Modified residuei142PhosphoserineCombined sources1
Modified residuei144PhosphoserineCombined sources1
Modified residuei166PhosphoserineCombined sources1
Cross-linki170Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei239PhosphoserineCombined sources1
Modified residuei241PhosphoserineCombined sources1
Modified residuei257PhosphoserineCombined sources1
Modified residuei277PhosphoserineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei294PhosphoserineCombined sources1
Modified residuei328PhosphoserineCombined sources1
Modified residuei354PhosphoserineCombined sources1
Modified residuei356PhosphoserineCombined sources1
Modified residuei366PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei385PhosphothreonineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei518PhosphoserineCombined sources1
Modified residuei519PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1
Modified residuei565PhosphoserineCombined sources1
Modified residuei569PhosphoserineCombined sources1
Modified residuei578PhosphoserineCombined sources1
Modified residuei580PhosphoserineCombined sources1
Modified residuei717N6-acetyllysineCombined sources1
Modified residuei849PhosphotyrosineCombined sources1
Modified residuei852PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by Clk1.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13523.
MaxQBiQ13523.
PaxDbiQ13523.
PeptideAtlasiQ13523.
PRIDEiQ13523.

PTM databases

iPTMnetiQ13523.
PhosphoSitePlusiQ13523.
SwissPalmiQ13523.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000112739.
CleanExiHS_PRPF4B.
ExpressionAtlasiQ13523. baseline and differential.
GenevisibleiQ13523. HS.

Organism-specific databases

HPAiHPA020638.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with Clk1 C-terminus.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARRB1P494072EBI-395940,EBI-743313
ARRB2P321213EBI-395940,EBI-714559
gagP045906EBI-395940,EBI-780156From a different organism.
KLF13Q9Y2Y95EBI-395940,EBI-1255893
POLR3FQ9H1D92EBI-395940,EBI-710067

Protein-protein interaction databases

BioGridi114416. 63 interactors.
IntActiQ13523. 42 interactors.
MINTiMINT-1379795.
STRINGi9606.ENSP00000337194.

Chemistry databases

BindingDBiQ13523.

Structurei

Secondary structure

11007
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi671 – 673Combined sources3
Turni684 – 686Combined sources3
Beta strandi687 – 694Combined sources8
Beta strandi697 – 699Combined sources3
Beta strandi700 – 706Combined sources7
Helixi707 – 709Combined sources3
Beta strandi713 – 718Combined sources6
Helixi724 – 742Combined sources19
Beta strandi753 – 759Combined sources7
Beta strandi762 – 767Combined sources6
Helixi774 – 781Combined sources8
Turni783 – 785Combined sources3
Helixi789 – 808Combined sources20
Helixi818 – 820Combined sources3
Beta strandi821 – 823Combined sources3
Beta strandi830 – 832Combined sources3
Beta strandi839 – 842Combined sources4
Helixi853 – 855Combined sources3
Helixi858 – 862Combined sources5
Helixi869 – 884Combined sources16
Helixi894 – 905Combined sources12
Helixi910 – 915Combined sources6
Turni920 – 922Combined sources3
Beta strandi929 – 932Combined sources4
Beta strandi943 – 947Combined sources5
Helixi955 – 959Combined sources5
Helixi967 – 983Combined sources17
Helixi988 – 990Combined sources3
Helixi994 – 998Combined sources5
Helixi1001 – 1004Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IANX-ray2.44A/B657-1007[»]
4IFCX-ray2.13A/B657-1007[»]
4IIRX-ray2.00A/B657-1007[»]
4IJPX-ray2.25A/B657-1007[»]
ProteinModelPortaliQ13523.
SMRiQ13523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini687 – 1006Protein kinasePROSITE-ProRule annotationAdd BLAST320

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 496Arg/Lys-rich (basic)Add BLAST458
Compositional biasi40 – 78His-richAdd BLAST39

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00760000119032.
HOVERGENiHBG055182.
InParanoidiQ13523.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG091G095F.
PhylomeDBiQ13523.
TreeFamiTF315246.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q13523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAETQSLR EQPEMEDANS EKSINEENGE VSEDQSQNKH SRHKKKKHKH
60 70 80 90 100
RSKHKKHKHS SEEDKDKKHK HKHKHKKHKR KEIIDASDKE GMSPAKRTKL
110 120 130 140 150
DDLALLEDLE KQRALIKAEL DNELMEGKVQ SGMGLILQGY ESGSEEEGEI
160 170 180 190 200
HEKARNGNRS STRSSSTKGK LELVDNKITT KKRSKSRSKE RTRHRSDKKK
210 220 230 240 250
SKGGIEIVKE KTTRSKSKER KKSKSPSKRS KSQDQARKSK SPTLRRRSQE
260 270 280 290 300
KIGKARSPTD DKVKIEDKSK SKDRKKSPII NESRSRDRGK KSRSPVDLRG
310 320 330 340 350
KSKDRRSRSK ERKSKRSETD KEKKPIKSPS KDASSGKENR SPSRRPGRSP
360 370 380 390 400
KRRSLSPKPR DKSRRSRSPL LNDRRSKQSK SPSRTLSPGR RAKSRSLERK
410 420 430 440 450
RREPERRRLS SPRTRPRDDI LSRRERSKDA SPINRWSPTR RRSRSPIRRR
460 470 480 490 500
SRSPLRRSRS PRRRSRSPRR RDRGRRSRSR LRRRSRSRGG RRRRSRSKVK
510 520 530 540 550
EDKFKGSLSE GMKVEQESSS DDNLEDFDVE EEDEEALIEQ RRIQRQAIVQ
560 570 580 590 600
KYKYLAEDSN MSVPSEPSSP QSSTRTRSPS PDDILERVAA DVKEYERENV
610 620 630 640 650
DTFEASVKAK HNLMTVEQNN GSSQKKLLAP DMFTESDDMF AAYFDSARLR
660 670 680 690 700
AAGIGKDFKE NPNLRDNWTD AEGYYRVNIG EVLDKRYNVY GYTGQGVFSN
710 720 730 740 750
VVRARDNARA NQEVAVKIIR NNELMQKTGL KELEFLKKLN DADPDDKFHC
760 770 780 790 800
LRLFRHFYHK QHLCLVFEPL SMNLREVLKK YGKDVGLHIK AVRSYSQQLF
810 820 830 840 850
LALKLLKRCN ILHADIKPDN ILVNESKTIL KLCDFGSASH VADNDITPYL
860 870 880 890 900
VSRFYRAPEI IIGKSYDYGI DMWSVGCTLY ELYTGKILFP GKTNNHMLKL
910 920 930 940 950
AMDLKGKMPN KMIRKGVFKD QHFDQNLNFM YIEVDKVTER EKVTVMSTIN
960 970 980 990 1000
PTKDLLADLI GCQRLPEDQR KKVHQLKDLL DQILMLDPAK RISINQALQH

AFIQEKI
Length:1,007
Mass (Da):116,987
Last modified:January 11, 2011 - v3
Checksum:iEC8C1538A61EDA78
GO

Sequence cautioni

The sequence AAH09844 differs from that shown. Reason: Frameshift at position 432.Curated
The sequence BAA25462 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti468P → T in AAH34969 (PubMed:15489334).Curated1
Sequence conflicti610K → R in BAF83933 (PubMed:14702039).Curated1
Sequence conflicti754F → L in AAH34969 (PubMed:15489334).Curated1
Sequence conflicti851V → F in AAH34969 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04696983I → V.Combined sources4 PublicationsCorresponds to variant rs9503893dbSNPEnsembl.1
Natural variantiVAR_047798584I → V.1 PublicationCorresponds to variant rs56267049dbSNPEnsembl.1
Natural variantiVAR_035633658F → L in a breast cancer sample; somatic mutation. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029347 mRNA. Translation: AAK38155.1.
AF283465 mRNA. Translation: AAM19101.1.
AB011108 mRNA. Translation: BAA25462.1. Different initiation.
AK291244 mRNA. Translation: BAF83933.1.
AL138831, AL033383 Genomic DNA. Translation: CAI20480.1.
AL033383, AL138831 Genomic DNA. Translation: CAI42121.1.
CH471087 Genomic DNA. Translation: EAW55146.1.
BC009844 mRNA. Translation: AAH09844.1. Frameshift.
BC034969 mRNA. Translation: AAH34969.1.
U48736 mRNA. Translation: AAB03268.1.
CCDSiCCDS4488.1.
RefSeqiNP_003904.3. NM_003913.4.
XP_016866899.1. XM_017011410.1.
XP_016866900.1. XM_017011411.1.
XP_016866901.1. XM_017011412.1.
XP_016866902.1. XM_017011413.1.
XP_016866903.1. XM_017011414.1.
XP_016866904.1. XM_017011415.1.
XP_016866905.1. XM_017011416.1.
XP_016866906.1. XM_017011417.1.
XP_016866907.1. XM_017011418.1.
XP_016866908.1. XM_017011419.1.
XP_016866909.1. XM_017011420.1.
XP_016866910.1. XM_017011421.1.
XP_016866911.1. XM_017011422.1.
XP_016866912.1. XM_017011423.1.
XP_016866913.1. XM_017011424.1.
XP_016866914.1. XM_017011425.1.
XP_016866915.1. XM_017011426.1.
XP_016866916.1. XM_017011427.1.
XP_016866917.1. XM_017011428.1.
XP_016866918.1. XM_017011429.1.
XP_016866919.1. XM_017011430.1.
XP_016866920.1. XM_017011431.1.
UniGeneiHs.159014.

Genome annotation databases

EnsembliENST00000337659; ENSP00000337194; ENSG00000112739.
ENST00000480058; ENSP00000433547; ENSG00000112739.
GeneIDi8899.
KEGGihsa:8899.
UCSCiuc003mvv.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029347 mRNA. Translation: AAK38155.1.
AF283465 mRNA. Translation: AAM19101.1.
AB011108 mRNA. Translation: BAA25462.1. Different initiation.
AK291244 mRNA. Translation: BAF83933.1.
AL138831, AL033383 Genomic DNA. Translation: CAI20480.1.
AL033383, AL138831 Genomic DNA. Translation: CAI42121.1.
CH471087 Genomic DNA. Translation: EAW55146.1.
BC009844 mRNA. Translation: AAH09844.1. Frameshift.
BC034969 mRNA. Translation: AAH34969.1.
U48736 mRNA. Translation: AAB03268.1.
CCDSiCCDS4488.1.
RefSeqiNP_003904.3. NM_003913.4.
XP_016866899.1. XM_017011410.1.
XP_016866900.1. XM_017011411.1.
XP_016866901.1. XM_017011412.1.
XP_016866902.1. XM_017011413.1.
XP_016866903.1. XM_017011414.1.
XP_016866904.1. XM_017011415.1.
XP_016866905.1. XM_017011416.1.
XP_016866906.1. XM_017011417.1.
XP_016866907.1. XM_017011418.1.
XP_016866908.1. XM_017011419.1.
XP_016866909.1. XM_017011420.1.
XP_016866910.1. XM_017011421.1.
XP_016866911.1. XM_017011422.1.
XP_016866912.1. XM_017011423.1.
XP_016866913.1. XM_017011424.1.
XP_016866914.1. XM_017011425.1.
XP_016866915.1. XM_017011426.1.
XP_016866916.1. XM_017011427.1.
XP_016866917.1. XM_017011428.1.
XP_016866918.1. XM_017011429.1.
XP_016866919.1. XM_017011430.1.
XP_016866920.1. XM_017011431.1.
UniGeneiHs.159014.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IANX-ray2.44A/B657-1007[»]
4IFCX-ray2.13A/B657-1007[»]
4IIRX-ray2.00A/B657-1007[»]
4IJPX-ray2.25A/B657-1007[»]
ProteinModelPortaliQ13523.
SMRiQ13523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114416. 63 interactors.
IntActiQ13523. 42 interactors.
MINTiMINT-1379795.
STRINGi9606.ENSP00000337194.

Chemistry databases

BindingDBiQ13523.
ChEMBLiCHEMBL1908382.
GuidetoPHARMACOLOGYi2177.

PTM databases

iPTMnetiQ13523.
PhosphoSitePlusiQ13523.
SwissPalmiQ13523.

Polymorphism and mutation databases

BioMutaiPRPF4B.
DMDMi317373526.

Proteomic databases

EPDiQ13523.
MaxQBiQ13523.
PaxDbiQ13523.
PeptideAtlasiQ13523.
PRIDEiQ13523.

Protocols and materials databases

DNASUi8899.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337659; ENSP00000337194; ENSG00000112739.
ENST00000480058; ENSP00000433547; ENSG00000112739.
GeneIDi8899.
KEGGihsa:8899.
UCSCiuc003mvv.4. human.

Organism-specific databases

CTDi8899.
DisGeNETi8899.
GeneCardsiPRPF4B.
H-InvDBHIX0005547.
HGNCiHGNC:17346. PRPF4B.
HPAiHPA020638.
MIMi602338. gene.
neXtProtiNX_Q13523.
OpenTargetsiENSG00000112739.
PharmGKBiPA38447.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00760000119032.
HOVERGENiHBG055182.
InParanoidiQ13523.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG091G095F.
PhylomeDBiQ13523.
TreeFamiTF315246.

Enzyme and pathway databases

BioCyciZFISH:HS03613-MONOMER.
BRENDAi2.7.11.1. 2681.
SignaLinkiQ13523.
SIGNORiQ13523.

Miscellaneous databases

ChiTaRSiPRPF4B. human.
GeneWikiiPRPF4B.
GenomeRNAii8899.
PROiQ13523.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112739.
CleanExiHS_PRPF4B.
ExpressionAtlasiQ13523. baseline and differential.
GenevisibleiQ13523. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP4B_HUMAN
AccessioniPrimary (citable) accession number: Q13523
Secondary accession number(s): A8K5C9
, Q5D0F6, Q5TAY8, Q8IVC3, Q8TDP2, Q96QT7, Q9UEE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 185 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.