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Protein

Protein phosphatase 1 regulatory subunit 1A

Gene

PPP1R1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.

GO - Molecular functioni

  • protein serine/threonine phosphatase inhibitor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 1A
Alternative name(s):
Protein phosphatase inhibitor 1
Short name:
I-1
Short name:
IPP-1
Gene namesi
Name:PPP1R1A
Synonyms:IPP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:9286. PPP1R1A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351T → A: No activity. 1 Publication
Mutagenesisi35 – 351T → D: 1000-fold reduction in activity, inhibits equally PP1 and PP2A. 1 Publication

Organism-specific databases

PharmGKBiPA33636.

Polymorphism and mutation databases

BioMutaiPPP1R1A.
DMDMi296452864.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 171171Protein phosphatase 1 regulatory subunit 1APRO_0000071477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei35 – 351Phosphothreonine; by PKA1 Publication
Modified residuei43 – 431PhosphoserineBy similarity
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei47 – 471PhosphoserineBy similarity
Modified residuei67 – 671PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation of Thr-35 is required for activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ13522.
PRIDEiQ13522.

PTM databases

iPTMnetiQ13522.
PhosphoSiteiQ13522.

Expressioni

Gene expression databases

BgeeiQ13522.
CleanExiHS_PPP1R1A.
ExpressionAtlasiQ13522. baseline and differential.
GenevisibleiQ13522. HS.

Organism-specific databases

HPAiCAB007811.
HPA041542.

Interactioni

Subunit structurei

Interacts with PPP1R15A.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP1R15AO758074EBI-1568511,EBI-714746

Protein-protein interaction databases

BioGridi111496. 14 interactions.
IntActiQ13522. 2 interactions.
STRINGi9606.ENSP00000257905.

Chemistry

BindingDBiQ13522.

Structurei

3D structure databases

ProteinModelPortaliQ13522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 124Essential for activity
Regioni42 – 5413Essential for activityCuratedAdd
BLAST
Regioni143 – 17129Interaction with PPP1R15AAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410J0ZP. Eukaryota.
ENOG41125ZM. LUCA.
HOGENOMiHOG000115665.
HOVERGENiHBG096069.
InParanoidiQ13522.
KOiK08050.
OrthoDBiEOG7XPZ7N.
PhylomeDBiQ13522.
TreeFamiTF332576.

Family and domain databases

InterProiIPR008466. PPI_1DARPP-32.
[Graphical view]
PANTHERiPTHR15417. PTHR15417. 1 hit.
PfamiPF05395. DARPP-32. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI
60 70 80 90 100
DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE
110 120 130 140 150
PEGAAESTET QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE
160 170
PSTKEPSTHI PPLDSKGANS V
Length:171
Mass (Da):19,011
Last modified:May 18, 2010 - v2
Checksum:i6F61FF08E4305729
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81R → Q in AAH22470 (PubMed:15489334).Curated
Sequence conflicti43 – 431S → G in AAL48321 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti109 – 1091E → G.4 Publications
Corresponds to variant rs1249958 [ dbSNP | Ensembl ].
VAR_053898
Natural varianti147 – 1471G → D.
Corresponds to variant rs34376731 [ dbSNP | Ensembl ].
VAR_053899

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48707 mRNA. Translation: AAB02402.1.
AY063766 mRNA. Translation: AAL48321.1.
CR457003 mRNA. Translation: CAG33284.1.
BC022470 mRNA. Translation: AAH22470.1.
CCDSiCCDS44912.1.
RefSeqiNP_006732.3. NM_006741.3.
UniGeneiHs.505662.

Genome annotation databases

EnsembliENST00000257905; ENSP00000257905; ENSG00000135447.
GeneIDi5502.
KEGGihsa:5502.
UCSCiuc001sgg.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48707 mRNA. Translation: AAB02402.1.
AY063766 mRNA. Translation: AAL48321.1.
CR457003 mRNA. Translation: CAG33284.1.
BC022470 mRNA. Translation: AAH22470.1.
CCDSiCCDS44912.1.
RefSeqiNP_006732.3. NM_006741.3.
UniGeneiHs.505662.

3D structure databases

ProteinModelPortaliQ13522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111496. 14 interactions.
IntActiQ13522. 2 interactions.
STRINGi9606.ENSP00000257905.

Chemistry

BindingDBiQ13522.

PTM databases

iPTMnetiQ13522.
PhosphoSiteiQ13522.

Polymorphism and mutation databases

BioMutaiPPP1R1A.
DMDMi296452864.

Proteomic databases

PaxDbiQ13522.
PRIDEiQ13522.

Protocols and materials databases

DNASUi5502.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257905; ENSP00000257905; ENSG00000135447.
GeneIDi5502.
KEGGihsa:5502.
UCSCiuc001sgg.3. human.

Organism-specific databases

CTDi5502.
GeneCardsiPPP1R1A.
HGNCiHGNC:9286. PPP1R1A.
HPAiCAB007811.
HPA041542.
MIMi613246. gene.
neXtProtiNX_Q13522.
PharmGKBiPA33636.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J0ZP. Eukaryota.
ENOG41125ZM. LUCA.
HOGENOMiHOG000115665.
HOVERGENiHBG096069.
InParanoidiQ13522.
KOiK08050.
OrthoDBiEOG7XPZ7N.
PhylomeDBiQ13522.
TreeFamiTF332576.

Miscellaneous databases

GenomeRNAii5502.
NextBioi21290.
PROiQ13522.
SOURCEiSearch...

Gene expression databases

BgeeiQ13522.
CleanExiHS_PPP1R1A.
ExpressionAtlasiQ13522. baseline and differential.
GenevisibleiQ13522. HS.

Family and domain databases

InterProiIPR008466. PPI_1DARPP-32.
[Graphical view]
PANTHERiPTHR15417. PTHR15417. 1 hit.
PfamiPF05395. DARPP-32. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitor."
    Endo S., Zhou X., Connor J., Wang B., Shenolikar S.
    Biochemistry 35:5220-5228(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-23 AND 53-61, MUTAGENESIS OF THR-35, VARIANT GLY-109.
    Tissue: Brain.
  2. "Cloning of inhibitor-1 of protein phosphatase type 1 from human heart."
    Mishra S., Tiwari N., Sabbah H.N., Gupta R.C.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-109.
    Tissue: Heart.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-109.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-109.
    Tissue: Brain.
  5. "Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1."
    Connor J.H., Weiser D.C., Li S., Hallenbeck J.M., Shenolikar S.
    Mol. Cell. Biol. 21:6841-6850(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP1R15A, PHOSPHORYLATION AT THR-35.
  6. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPPR1A_HUMAN
AccessioniPrimary (citable) accession number: Q13522
Secondary accession number(s): Q6IB01, Q8TBJ2, Q8WWV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 18, 2010
Last modified: March 16, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.