Q13519 (PNOC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prepronociceptin

Cleaved into the following 3 chains:

1. Neuropeptide 1
2. Nociceptin
   Alternative name(s):
   Orphanin FQ
   PPNOC
3. Neuropeptide 2

Gene names

Name:	PNOC
Synonyms:	OFQ

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development By similarity.
Subcellular location	Secreted.
Tissue specificity	Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation. Ref.3
Post-translational modification	Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Neuropeptide Neurotransmitter Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW sensory perception Traceable author statement PubMed 9521323. Source: ProtInc signal transduction Traceable author statement PubMed 9521323. Source: ProtInc synaptic transmission Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Traceable author statement. Source: Reactome
Molecular_function	neuropeptide hormone activity Traceable author statement PubMed 10419552. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 95	76			PRO_0000008325
	Peptide	98 – 127	30	Neuropeptide 1 Probable		PRO_0000008326
	Peptide	130 – 146	17	Nociceptin		PRO_0000008327
	Peptide	149 – 165	17	Neuropeptide 2 Probable		PRO_0000008328
	Propeptide	169 – 176	8			PRO_0000008329

Sequences

	Sequence		Length	Mass (Da)
	Q13519 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8FC8DB4BB094CE6A	FASTA	176	20,295
	10 20 30 40 50 60 MKVLLCDLLL LSLFSSVFSS CQRDCLTCQE KLHPALDSFD LEVCILECEE KVFPSPLWTP 70 80 90 100 110 120 CTKVMARSSW QLSPAAPEHV AAALYQPRAS EMQHLRRMPR VRSLFQEQEE PEPGMEEAGE 130 140 150 160 170 MEQKQLQKRF GGFTGARKSA RKLANQKRFS EFMRQYLVLS MQSSQRRRTL HQNGNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene." Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M. Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Primary structure and tissue distribution of the orphanin FQ precursor." Nothacker H.-P., Reinscheid R.K., Mansour A., Henningsen R.A., Ardati A., Monsma F.J. Jr., Watson S.J., Civelli O. Proc. Natl. Acad. Sci. U.S.A. 93:8677-8682(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Human neutrophils as a source of nociceptin: a novel link between pain and inflammation." Fiset M.E., Gilbert C., Poubelle P.E., Pouliot M. Biochemistry 42:10498-10505(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[8]	"Solution conformation of nociceptin." Salvadori S., Picone D., Tancredi T., Guerrini R., Spadaccini R., Lazarus L.H., Regoli D., Temussi P.A. Biochem. Biophys. Res. Commun. 233:640-643(1997) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF NOCICEPTIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97370 mRNA. Translation: CAA66040.1. X97367, X97368 Genomic DNA. Translation: CAA66039.1. U48263 mRNA. Translation: AAC50651.1. AY335948 mRNA. Translation: AAP94601.1. BT019334 mRNA. Translation: AAV38141.1. CR541940 mRNA. Translation: CAG46738.1. CR541965 mRNA. Translation: CAG46763.1. CH471080 Genomic DNA. Translation: EAW63526.1. CH471080 Genomic DNA. Translation: EAW63527.1. BC034758 mRNA. Translation: AAH34758.1.
IPI	IPI00013701.
PIR	JC6152.
RefSeq	NP_006219.1. NM_006228.3.
UniGene	Hs.88218.
3D structure databases
ProteinModelPortal	Q13519.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000301908.
PTM databases
PhosphoSite	Q13519.
Polymorphism databases
DMDM	2494348.
Proteomic databases
PaxDb	Q13519.
PRIDE	Q13519.
Protocols and materials databases
DNASU	5368.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301908; ENSP00000301908; ENSG00000168081.
GeneID	5368.
KEGG	hsa:5368.
UCSC	uc003xgp.3. human.
Organism-specific databases
CTD	5368.
GeneCards	GC08P028174.
HGNC	HGNC:9163. PNOC.
HPA	HPA044507.
MIM	601459. gene.
neXtProt	NX_Q13519.
PharmGKB	PA33485.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG40726.
HOGENOM	HOG000082421.
HOVERGEN	HBG008227.
InParanoid	Q13519.
OMA	HLKRMPR.
OrthoDB	EOG44BB3B.
PhylomeDB	Q13519.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q13519.
Bgee	Q13519.
CleanEx	HS_PNOC.
Genevestigator	Q13519.
GermOnline	ENSG00000168081. Homo sapiens.
Family and domain databases
InterPro	IPR002367. Nociceptin. IPR006024. Opioid_neupept. [Graphical view]
PANTHER	PTHR11438. PTHR11438. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01031. ORPHNNPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5368.
NextBio	20808.
SOURCE	Search...

Entry information

Entry name

PNOC_HUMAN

Accession

Primary (citable) accession number: Q13519
Secondary accession number(s): Q6FH16

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.