Q13516 (OLIG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligodendrocyte transcription factor 2
Short name=Oligo2

Alternative name(s):
Class B basic helix-loop-helix protein 1
Short name=bHLHb1
Class E basic helix-loop-helix protein 19
Short name=bHLHe19
Protein kinase C-binding protein 2
Protein kinase C-binding protein RACK17

Gene names

Name:	OLIG2
Synonyms:	BHLHB1, BHLHE19, PRKCBP2, RACK17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development By similarity.
Subunit structure	Interacts with NKX2-2 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2 By similarity.
Tissue specificity	Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. Ref.5 Ref.6
Induction	By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain By similarity.
Domain	The bHLH is essential for interaction with NKX2-2 By similarity.
Involvement in disease	A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.
Sequence caution	The sequence AAC72247.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF61215.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Chromosomal rearrangement
Disease	Proto-oncogene
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	myelination Inferred from electronic annotation. Source: Compara negative regulation of neuron differentiation Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neuron fate commitment Inferred from electronic annotation. Source: Compara positive regulation of oligodendrocyte differentiation Inferred from electronic annotation. Source: Compara spinal cord motor neuron differentiation Inferred from electronic annotation. Source: Compara spinal cord oligodendrocyte cell fate specification Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Oligodendrocyte transcription factor 2		PRO_0000127414
Regions
	Domain	108 – 162	55	bHLH
	Compositional bias	77 – 88	12	Poly-Ser
	Compositional bias	206 – 212	7	Poly-Ala
	Compositional bias	225 – 237	13	Poly-Ala
	Compositional bias	266 – 270	5	Poly-Ala
	Compositional bias	273 – 280	8	Poly-Gly
Experimental info
	Sequence conflict	150	1	A → T in AAH47511. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q13516 [UniParc]. Last modified August 13, 2002. Version 2. Checksum: 0EC9223961062509	FASTA	323	32,385
	10 20 30 40 50 60 MDSDASLVSS RPSSPEPDDL FLPARSKGSS GSAFTGGTVS SSTPSDCPPE LSAELRGAMG 70 80 90 100 110 120 SAGAHPGDKL GGSGFKSSSS STSSSTSSAA ASSTKKDKKQ MTEPELQQLR LKINSRERKR 130 140 150 160 170 180 MHDLNIAMDG LREVMPYAHG PSVRKLSKIA TLLLARNYIL MLTNSLEEMK RLVSEIYGGH 190 200 210 220 230 240 HAGFHPSACG GLAHSAPLPA ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS 250 260 270 280 290 300 ASLPGSGLPS VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG ASGGFQHWGG MPCPCSMCQV 310 320 PPPHHHVSAM GAGSLPRLTS DAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein kinase C-binding protein." Kuroda S., Tokunaga C., Kiyohara Y., Konishi H., Kikkawa U. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hippocampus.
[2]	"The t(14;21)(q11.2;q22) chromosomal translocation associated with T-cell acute lymphoblastic leukemia activates the BHLHB1 gene." Wang J., Jani-Sait S.N., Escalon E.A., Carroll A.J., de Jong P.J., Kirsch I.R., Aplan P.D. Proc. Natl. Acad. Sci. U.S.A. 97:3497-3502(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"OLIG2 as a specific marker of oligodendroglial tumour cells." Marie Y., Sanson M., Mokhtari K., Leuraud P., Kujas M., Delattre J.-Y., Poirier J., Zalc B., Hoang-Xuan K. Lancet 358:298-300(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Oligodendrocyte lineage genes (OLIG) as molecular markers for human glial brain tumors." Lu Q.R., Park J.K., Noll E., Chan J.A., Alberta J.A., Yuk D.-I., Alzamora M.G., Louis D.N., Stiles C.D., Rowitch D.H., Black P.M. Proc. Natl. Acad. Sci. U.S.A. 98:10851-10856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48250 mRNA. Translation: AAC72247.1. Different initiation. AF221520 mRNA. Translation: AAF61215.1. Different initiation. AK091462 mRNA. Translation: BAG52365.1. BC047511 mRNA. Translation: AAH47511.1.
IPI	IPI00013699.
PIR	G02409.
RefSeq	NP_005797.1. NM_005806.3.
UniGene	Hs.176977. Hs.732068.
3D structure databases
ProteinModelPortal	Q13516.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13516. 1 interaction.
STRING	9606.ENSP00000331040.
PTM databases
PhosphoSite	Q13516.
Polymorphism databases
DMDM	22261817.
Proteomic databases
PaxDb	Q13516.
PRIDE	Q13516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000333337; ENSP00000331040; ENSG00000205927. ENST00000382357; ENSP00000371794; ENSG00000205927.
GeneID	10215.
KEGG	hsa:10215.
UCSC	uc002yqx.2. human.
Organism-specific databases
CTD	10215.
GeneCards	GC21P034398.
H-InvDB	HIX0016071.
HGNC	HGNC:9398. OLIG2.
HPA	CAB019381. HPA003254.
MIM	606386. gene.
neXtProt	NX_Q13516.
PharmGKB	PA31919.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG313428.
HOGENOM	HOG000015765.
HOVERGEN	HBG053370.
InParanoid	Q13516.
KO	K09085.
OMA	SFQHWGG.
OrthoDB	EOG4RJG2Q.
PhylomeDB	Q13516.
Gene expression databases
ArrayExpress	Q13516.
Bgee	Q13516.
CleanEx	HS_OLIG2.
Genevestigator	Q13516.
GermOnline	ENSG00000205927. Homo sapiens.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10215.
NextBio	38678.
SOURCE	Search...

Entry information

Entry name

OLIG2_HUMAN

Accession

Primary (citable) accession number: Q13516
Secondary accession number(s): B3KRF3, Q86X04, Q9NZ14

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 13, 2002
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.