Reviewed, UniProtKB/Swiss-Prot Q13509 (TBB3_HUMAN)

Names and origin

Protein names

Recommended name:
    Tubulin beta-3 chain
Alternative name(s):
    Tubulin beta-III
    Tubulin beta-4

Gene names

Name:	TUBB3
Synonyms:	TUBB4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit structure	Dimer of alpha and beta chains.
Domain	The highly acidic C-terminal region may bind cations such as calcium.
Post-translational modification	Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules Probable.
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Microtubule
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	microtubule-based movement Inferred from electronic annotation. Source: InterPro protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Tubulin beta-3 chain		PRO_0000048250
Regions
	Nucleotide binding	140 – 146	7	GTP Potential
Amino acid modifications
	Modified residue	58	1	N6-acetyllysine Ref.7
Experimental info
	Sequence conflict	275	1	A → R in AAC52035. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q13509-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 4B9CDE7DBA102949	FASTA	450	50,433
	10 20 30 40 50 60 MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPSGNYVGDS DLQLERISVY YNEASSHKYV 70 80 90 100 110 120 PRAILVDLEP GTMDSVRSGA FGHLFRPDNF IFGQSGAGNN WAKGHYTEGA ELVDSVLDVV 130 140 150 160 170 180 RKECENCDCL QGFQLTHSLG GGTGSGMGTL LISKVREEYP DRIMNTFSVV PSPKVSDTVV 190 200 210 220 230 240 EPYNATLSIH QLVENTDETY CIDNEALYDI CFRTLKLATP TYGDLNHLVS ATMSGVTTSL 250 260 270 280 290 300 RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTARGSQQ YRALTVPELT QQMFDAKNMM 310 320 330 340 350 360 AACDPRHGRY LTVATVFRGR MSMKEVDEQM LAIQSKNSSY FVEWIPNNVK VAVCDIPPRG 370 380 390 400 410 420 LKMSSTFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS 430 440 450 EYQQYQDATA EEEGEMYEDD EEESEAQGPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of human betaIII-tubulin cDNA: induction of betaIII isotype in human prostate carcinoma cells by acute exposure to antimicrotubule agents." Ranganathan S., Dexter D.W., Benetatos C.A., Hudes G.R. Biochim. Biophys. Acta 1395:237-245(1998) [PubMed: 9473684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Banerjee A. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary cancer.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 63-77; 104-121; 242-251; 163-174 AND 381-390, MASS SPECTROMETRY. Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation." Rogowski K., Juge F., van Dijk J., Wloga D., Strub J.-M., Levilliers N., Thomas D., Bre M.-H., Van Dorsselaer A., Gaertig J., Janke C. Cell 137:1076-1087(2009) [PubMed: 19524510] [Abstract] Cited for: GLYCYLATION.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-58, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47634 mRNA. Translation: AAC52035.1. AF427491 mRNA. Translation: AAL28094.1. BC000748 mRNA. Translation: AAH00748.1. BC003021 mRNA. Translation: AAH03021.2.
IPI	IPI00013683.
RefSeq	NP_006077.2.
UniGene	Hs.511743
3D structure databases
HSSP	HSSP built from PDB template 1FFX based on UniProtKB P02554.
SMR	Q13509. Positions 2-427.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13509. 4 interactions.
STRING	Q13509.
PTM databases
PhosphoSite	Q13509.
2-D gel databases
OGP	Q13509.
Proteomic databases
PRIDE	Q13509.
Genome annotation databases
Ensembl	ENST00000304984; ENSP00000302777; ENSG00000198211; Homo sapiens. [Genome view] ENST00000315491; ENSP00000320295; ENSG00000198211; Homo sapiens. [Genome view]
GeneID	10381.
KEGG	hsa:10381.
UCSC	uc002fph.1. human.
Organism-specific databases
CTD	10381.
GeneCards	GC16P088514.
HGNC	HGNC:20772. TUBB3.
HPA	CAB011512.
MIM	602661. gene.
PharmGKB	PA134953867.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q13509.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q13509.
Bgee	Q13509.
CleanEx	HS_TUBB3. HS_TUBB4.
Genevestigator	Q13509.
GermOnline	ENSG00000198211. Homo sapiens.
Family and domain databases
InterPro	IPR013838. Beta-tubulin_BS. IPR002453. Beta_tubulin. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
PANTHER	PTHR11588:SF9. Beta_tubulin. 1 hit. PTHR11588. Tubulin. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01163. BETATUBULIN. PR01161. TUBULIN.
PROSITE	PS00227. TUBULIN. 1 hit. PS00228. TUBULIN_B_AUTOREG. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39327.
SOURCE	Search...

Entry information

Entry name

TBB3_HUMAN

Accession

Primary (citable) accession number: Q13509
Secondary accession number(s): Q9BTZ0, Q9BW10

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 2, 2002
Last modified:	October 13, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.