Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ecto-ADP-ribosyltransferase 3

Gene

ART3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011NADBy similarity
Binding sitei163 – 1631NADBy similarity
Active sitei229 – 2291By similarity

GO - Molecular functioni

  • NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: UniProtKB-EC
  • NAD+ ADP-ribosyltransferase activity Source: ProtInc

GO - Biological processi

  • protein ADP-ribosylation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Ecto-ADP-ribosyltransferase 3 (EC:2.4.2.31)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 3
Short name:
ARTC3
Mono(ADP-ribosyl)transferase 3
NAD(P)(+)--arginine ADP-ribosyltransferase 3
Gene namesi
Name:ART3
Synonyms:TMART
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:725. ART3.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular exosome Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25016.

Polymorphism and mutation databases

BioMutaiART3.
DMDMi22261808.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 362336Ecto-ADP-ribosyltransferase 3PRO_0000019325Add
BLAST
Propeptidei363 – 38927Removed in mature formBy similarityPRO_0000019326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 256By similarity
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence analysis
Glycosylationi346 – 3461O-linked (GalNAc...)1 Publication
Lipidationi362 – 3621GPI-anchor amidated serineBy similarity

Post-translational modificationi

O-glycosylated with core 1 or possibly core 8 glycans.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ13508.
PeptideAtlasiQ13508.
PRIDEiQ13508.

PTM databases

iPTMnetiQ13508.
UniCarbKBiQ13508.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiQ13508.
CleanExiHS_ART3.
ExpressionAtlasiQ13508. baseline and differential.
GenevisibleiQ13508. HS.

Organism-specific databases

HPAiHPA011268.

Interactioni

Protein-protein interaction databases

BioGridi106912. 24 interactions.
IntActiQ13508. 1 interaction.
STRINGi9606.ENSP00000348064.

Structurei

3D structure databases

ProteinModelPortaliQ13508.
SMRiQ13508. Positions 28-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati283 – 292101
Repeati293 – 302102
Repeati303 – 312103

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni283 – 312303 X 10 AA tandem repeats of [GS]-E-K-N-[QW]-K-L-E-D-HAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410J8VA. Eukaryota.
ENOG411167J. LUCA.
GeneTreeiENSGT00530000062975.
HOGENOMiHOG000069954.
HOVERGENiHBG006445.
InParanoidiQ13508.
KOiK00775.
OMAiFTFGGLN.
PhylomeDBiQ13508.
TreeFamiTF335356.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 3 (identifier: Q13508-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTGHFEIVT MLLATMILVD IFQVKAEVLD MADNAFDDEY LKCTDRMEIK
60 70 80 90 100
YVPQLLKEEK ASHQQLDTVW ENAKAKWAAR KTQIFLPMNF KDNHGIALMA
110 120 130 140 150
YISEAQEQTP FYHLFSEAVK MAGQSREDYI YGFQFKAFHF YLTRALQLLR
160 170 180 190 200
KPCEASSKTV VYRTSQGTSF TFGGLNQARF GHFTLAYSAK PQAANDQLTV
210 220 230 240 250
LSIYTCLGVD IENFLDKESE RITLIPLNEV FQVSQEGAGN NLILQSINKT
260 270 280 290 300
CSHYECAFLG GLKTENCIEN LEYFQPIYVY NPGEKNQKLE DHSEKNWKLE
310 320 330 340 350
DHGEKNQKLE DHGVKILEPT QIPGMKIPEP FPLPEDKSQG NINNPTPGPV
360 370 380
PVPGPKSHPS ASSGKLLLPQ FGMVIILISV SAINLFVAL
Length:389
Mass (Da):43,923
Last modified:August 13, 2002 - v2
Checksum:iEE1311304BB2F41D
GO
Isoform 1 (identifier: Q13508-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-346: GMKIPEPFPLPEDKSQGNINNPT → A

Show »
Length:367
Mass (Da):41,489
Checksum:i2741C884A7C685A2
GO
Isoform 2 (identifier: Q13508-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-334: Missing.

Show »
Length:378
Mass (Da):42,716
Checksum:i210DDA8EB3ABEA56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571S → C in CAA65096 (PubMed:9119374).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti363 – 3631S → L.3 Publications
Corresponds to variant rs1128864 [ dbSNP | Ensembl ].
VAR_060072

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei324 – 34623GMKIP…INNPT → A in isoform 1. 1 PublicationVSP_003375Add
BLAST
Alternative sequencei324 – 33411Missing in isoform 2. 1 PublicationVSP_003374Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47054 mRNA. Translation: AAB01894.1.
X95827 Genomic DNA. Translation: CAA65096.1.
BT007249 mRNA. Translation: AAP35913.1.
CR541664 mRNA. Translation: CAG46465.1.
CH471057 Genomic DNA. Translation: EAX05765.1.
BC008397 mRNA. Translation: AAH08397.1.
BC017913 mRNA. Translation: AAH17913.1.
CCDSiCCDS3575.1. [Q13508-3]
CCDS47079.1. [Q13508-1]
CCDS47080.1. [Q13508-2]
PIRiS62906.
RefSeqiNP_001123488.1. NM_001130016.2. [Q13508-1]
NP_001123489.1. NM_001130017.2. [Q13508-2]
NP_001170.2. NM_001179.5. [Q13508-3]
UniGeneiHs.24976.
Hs.731997.

Genome annotation databases

EnsembliENST00000341029; ENSP00000343843; ENSG00000156219. [Q13508-2]
ENST00000349321; ENSP00000304313; ENSG00000156219. [Q13508-3]
ENST00000355810; ENSP00000348064; ENSG00000156219. [Q13508-1]
GeneIDi419.
KEGGihsa:419.
UCSCiuc003hjk.4. human. [Q13508-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47054 mRNA. Translation: AAB01894.1.
X95827 Genomic DNA. Translation: CAA65096.1.
BT007249 mRNA. Translation: AAP35913.1.
CR541664 mRNA. Translation: CAG46465.1.
CH471057 Genomic DNA. Translation: EAX05765.1.
BC008397 mRNA. Translation: AAH08397.1.
BC017913 mRNA. Translation: AAH17913.1.
CCDSiCCDS3575.1. [Q13508-3]
CCDS47079.1. [Q13508-1]
CCDS47080.1. [Q13508-2]
PIRiS62906.
RefSeqiNP_001123488.1. NM_001130016.2. [Q13508-1]
NP_001123489.1. NM_001130017.2. [Q13508-2]
NP_001170.2. NM_001179.5. [Q13508-3]
UniGeneiHs.24976.
Hs.731997.

3D structure databases

ProteinModelPortaliQ13508.
SMRiQ13508. Positions 28-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106912. 24 interactions.
IntActiQ13508. 1 interaction.
STRINGi9606.ENSP00000348064.

PTM databases

iPTMnetiQ13508.
UniCarbKBiQ13508.

Polymorphism and mutation databases

BioMutaiART3.
DMDMi22261808.

Proteomic databases

PaxDbiQ13508.
PeptideAtlasiQ13508.
PRIDEiQ13508.

Protocols and materials databases

DNASUi419.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341029; ENSP00000343843; ENSG00000156219. [Q13508-2]
ENST00000349321; ENSP00000304313; ENSG00000156219. [Q13508-3]
ENST00000355810; ENSP00000348064; ENSG00000156219. [Q13508-1]
GeneIDi419.
KEGGihsa:419.
UCSCiuc003hjk.4. human. [Q13508-1]

Organism-specific databases

CTDi419.
GeneCardsiART3.
HGNCiHGNC:725. ART3.
HPAiHPA011268.
MIMi603086. gene.
neXtProtiNX_Q13508.
PharmGKBiPA25016.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J8VA. Eukaryota.
ENOG411167J. LUCA.
GeneTreeiENSGT00530000062975.
HOGENOMiHOG000069954.
HOVERGENiHBG006445.
InParanoidiQ13508.
KOiK00775.
OMAiFTFGGLN.
PhylomeDBiQ13508.
TreeFamiTF335356.

Miscellaneous databases

ChiTaRSiART3. human.
GeneWikiiART3.
GenomeRNAii419.
PROiQ13508.
SOURCEiSearch...

Gene expression databases

BgeeiQ13508.
CleanExiHS_ART3.
ExpressionAtlasiQ13508. baseline and differential.
GenevisibleiQ13508. HS.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human testis specifically expresses a homologue of the rodent T lymphocytes RT6 mRNA."
    Levy I., Wu Y.-Q., Roeckel N., Bulle F., Pawlak A., Siegrist S., Mattei M.-G., Guellaen G.
    FEBS Lett. 382:276-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Testis.
  2. "Two novel human members of an emerging mammalian gene family related to mono-ADP-ribosylating bacterial toxins."
    Koch-Nolte F., Haag F., Braren R., Kuehl M., Hoovers J., Balasubramanian S., Bazan J.F., Thiele H.-G.
    Genomics 39:370-376(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-363.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-363.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT LEU-363.
    Tissue: Skeletal muscle.
  7. "Toward a unified nomenclature for mammalian ADP-ribosyltransferases."
    Hottiger M.O., Hassa P.O., Luscher B., Schuler H., Koch-Nolte F.
    Trends Biochem. Sci. 35:208-219(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  8. "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
    Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G.
    Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT THR-346, STRUCTURE OF CARBOHYDRATES, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNAR3_HUMAN
AccessioniPrimary (citable) accession number: Q13508
Secondary accession number(s): Q53XW3
, Q6FHT7, Q8WVJ7, Q93069, Q96HL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 13, 2002
Last modified: June 8, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.