Short transient receptor potential channel 3

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Q13507 (TRPC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Short transient receptor potential channel 3
Short name=TrpC3

Alternative name(s):
Transient receptor protein 3
Short name=TRP-3
Short name=hTrp-3
Short name=hTrp3

Gene names

Name:	TRPC3
Synonyms:	TRP3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. Ref.1 Ref.6 Ref.8 Ref.12
Subunit structure	Interacts with TRPC1, ITPR1, ITPR3, MX1 and RNF24. Interacts with JPH2; the interaction is involved in maintaining Ca²⁺ homeostasis in skeletal muscle and is mediated by JPH2 'Ser-165' phosphorylation. Ref.7 Ref.9 Ref.10 Ref.11 Ref.12
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis.
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC3 sub-subfamily. [View classification] Contains 4 ANK repeats.
Sequence caution	The sequence AAC51653.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH93682.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH93684.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA74083.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	ANK repeat Repeat Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ionic channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axon guidance Traceable author statement. Source: Reactome phototransduction Traceable author statement. Source: ProtInc platelet activation Traceable author statement. Source: Reactome
Cellular component	integral to plasma membrane Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction Ref.12. Source: UniProtKB store-operated calcium channel activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ITPR3	Q14573	5	EBI-520807,EBI-351055
MX1	P20591	2	EBI-520807,EBI-929476
Plcg1	P10686	2	EBI-520807,EBI-520788	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 836

836

Short transient receptor potential channel 3

PRO_0000215310

Regions

Topological domain

1 – 337

337

Cytoplasmic Potential

Transmembrane

338 – 358

Helical; Potential

Topological domain

359 – 369

Extracellular Potential

Transmembrane

370 – 390

Helical; Potential

Topological domain

391 – 418

Cytoplasmic Potential

Transmembrane

419 – 439

Helical; Potential

Topological domain

440 – 451

Extracellular Potential

Transmembrane

452 – 472

Helical; Potential

Topological domain

473 – 523

Cytoplasmic Potential

Transmembrane

524 – 544

Helical; Potential

Topological domain

545 – 567

Extracellular Potential

Transmembrane

568 – 588

Helical; Potential

Topological domain

589 – 603

Cytoplasmic Potential

Transmembrane

604 – 624

Helical; Potential

Topological domain

625 – 637

Extracellular Potential

Transmembrane

638 – 658

Helical; Potential

Topological domain

659 – 694

Cytoplasmic Potential

Transmembrane

695 – 714

Helical; Potential

Topological domain

715 – 836

122

Extracellular Potential

Repeat

26 – 55

ANK 1

Repeat

61 – 90

ANK 2

Repeat

92 – 118

ANK 3

Repeat

147 – 176

ANK 4

Region

765 – 785

Binds to IP3R3

Experimental info

Sequence conflict

727 – 730

EMGM → GNGEW in CAA61448. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q13507 [UniParc].

Last modified May 3, 2011. Version 3.
Checksum: 4179CB3DA8B3F508
Show »

FASTA

836

96,009

        10         20         30         40         50         60 
MREKGRRQAV RGPAFMFNDR GTSLTAEEER FLDAAEYGNI PVVRKMLEES KTLNVNCVDY 

        70         80         90        100        110        120 
MGQNALQLAV GNEHLEVTEL LLKKENLARI GDALLLAISK GYVRIVEAIL NHPGFAASKR 

       130        140        150        160        170        180 
LTLSPCEQEL QDDDFYAYDE DGTRFSPDIT PIILAAHCQK YEVVHMLLMK GARIERPHDY 

       190        200        210        220        230        240 
FCKCGDCMEK QRHDSFSHSR SRINAYKGLA SPAYLSLSSE DPVLTALELS NELAKLANIE 

       250        260        270        280        290        300 
KEFKNDYRKL SMQCKDFVVG VLDLCRDSEE VEAILNGDLE SAEPLEVHRH KASLSRVKLA 

       310        320        330        340        350        360 
IKYEVKKFVA HPNCQQQLLT IWYENLSGLR EQTIAIKCLV VLVVALGLPF LAIGYWIAPC 

       370        380        390        400        410        420 
SRLGKILRSP FMKFVAHAAS FIIFLGLLVF NASDRFEGIT TLPNITVTDY PKQIFRVKTT 

       430        440        450        460        470        480 
QFTWTEMLIM VWVLGMMWSE CKELWLEGPR EYILQLWNVL DFGMLSIFIA AFTARFLAFL 

       490        500        510        520        530        540 
QATKAQQYVD SYVQESDLSE VTLPPEIQYF TYARDKWLPS DPQIISEGLY AIAVVLSFSR 

       550        560        570        580        590        600 
IAYILPANES FGPLQISLGR TVKDIFKFMV LFIMVFFAFM IGMFILYSYY LGAKVNAAFT 

       610        620        630        640        650        660 
TVEESFKTLF WSIFGLSEVT SVVLKYDHKF IENIGYVLYG IYNVTMVVVL LNMLIAMINS 

       670        680        690        700        710        720 
SYQEIEDDSD VEWKFARSKL WLSYFDDGKT LPPPFSLVPS PKSFVYFIMR IVNFPKCRRR 

       730        740        750        760        770        780 
RLQKDIEMGM GNSKSRLNLF TQSNSRVFES HSFNSILNQP TRYQQIMKRL IKRYVLKAQV 

       790        800        810        820        830 
DKENDEVNEG ELKEIKQDIS SLRYELLEDK SQATEELAIL IHKLSEKLNP SMLRCE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." Zhu X., Jiang M., Peyton M., Boulay G., Hurst R., Stefani E., Birnbaumer L. Cell 85:661-671(1996) [PubMed: 8646775] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Embryonic kidney.
[2]	"Coassembly of TRP and TRPL produces a distinct store-operated conductance." Xu X.-Z.S., Li H.-S., Guggino W.B., Montell C. Cell 89:1155-1164(1997) [PubMed: 9215637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"TRPC1, a human homolog of a Drosophila store-operated channel." Wes P.D., Chevesich J., Jeromin A., Rosenberg C., Stetten G., Montell C. Proc. Natl. Acad. Sci. U.S.A. 92:9652-9656(1995) [PubMed: 7568191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 620-735. Tissue: Fetal brain.
[5]	"The membrane topology of human transient receptor potential 3 as inferred from glycosylation-scanning mutagenesis and epitope immunocytochemistry." Vannier B., Zhu X., Brown D., Birnbaumer L. J. Biol. Chem. 273:8675-8679(1998) [PubMed: 9535843] [Abstract] Cited for: TOPOLOGY.
[6]	"Receptor-activated Ca2+ influx via human Trp3 stably expressed in human embryonic kidney (HEK)293 cells. Evidence for a non-capacitative Ca2+ entry." Zhu X., Jiang M., Birnbaumer L. J. Biol. Chem. 273:133-142(1998) [PubMed: 9417057] [Abstract] Cited for: FUNCTION.
[7]	"Functional interaction between InsP3 receptors and store-operated Htrp3 channels." Kiselyov K., Xu X., Mozhayeva G., Kuo T., Pessah I., Mignery G., Zhu X., Birnbaumer L., Muallem S. Nature 396:478-482(1998) [PubMed: 9853757] [Abstract] Cited for: INTERACTION WITH ITPR1.
[8]	"Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol." Hofmann T., Obukhov A.G., Schaefer M., Harteneck C., Gudermann T., Schultz G. Nature 397:259-263(1999) [PubMed: 9930701] [Abstract] Cited for: FUNCTION.
[9]	"Modulation of Ca(2+) entry by polypeptides of the inositol 1,4, 5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP): evidence for roles of TRP and IP3R in store depletion-activated Ca(2+) entry." Boulay G., Brown D.M., Qin N., Jiang M., Dietrich A., Zhu M.X., Chen Z., Birnbaumer M., Mikoshiba K., Birnbaumer L. Proc. Natl. Acad. Sci. U.S.A. 96:14955-14960(1999) [PubMed: 10611319] [Abstract] Cited for: INTERACTION WITH ITPR3.
[10]	"MxA, a member of the dynamin superfamily, interacts with the ankyrin-like repeat domain of TRPC." Lussier M.P., Cayouette S., Lepage P.K., Bernier C.L., Francoeur N., St-Hilaire M., Pinard M., Boulay G. J. Biol. Chem. 280:19393-19400(2005) [PubMed: 15757897] [Abstract] Cited for: INTERACTION WITH MX1.
[11]	"RNF24, a new TRPC interacting protein, causes the intracellular retention of TRPC." Lussier M.P., Lepage P.K., Bousquet S.M., Boulay G. Cell Calcium 43:432-443(2008) [PubMed: 17850865] [Abstract] Cited for: INTERACTION WITH RNF24.
[12]	"S165F mutation of junctophilin 2 affects Ca2+ signalling in skeletal muscle." Woo J.S., Hwang J.H., Ko J.K., Weisleder N., Kim do H., Ma J., Lee E.H. Biochem. J. 427:125-134(2010) [PubMed: 20095964] [Abstract] Cited for: INTERACTION WITH JPH2, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U47050 mRNA. Translation: AAC51653.1. Different initiation. Y13758 mRNA. Translation: CAA74083.1. Different initiation. BC093682 mRNA. Translation: AAH93682.1. Different initiation. BC093684 mRNA. Translation: AAH93684.1. Different initiation. X89068 mRNA. Translation: CAA61448.1.
IPI	IPI00748783.
RefSeq	NP_001124170.1. NM_001130698.1. NP_003296.1. NM_003305.2.
UniGene	Hs.150981.
3D structure databases
ProteinModelPortal	Q13507.
SMR	Q13507. Positions 30-179.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q13507. 3 interactions.
STRING	Q13507.
Protein family/group databases
TCDB	1.A.4.1.4. transient receptor potential Ca2+ channel (TRP-CC) family.
PTM databases
PhosphoSite	Q13507.
Polymorphism databases
DMDM	12643721.
Proteomic databases
PRIDE	Q13507.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264811; ENSP00000264811; ENSG00000138741. ENST00000379645; ENSP00000368966; ENSG00000138741.
GeneID	7222.
KEGG	hsa:7222.
UCSC	uc003ief.1. human.
Organism-specific databases
CTD	7222.
GeneCards	GC04M122800.
H-InvDB	HIX0031423.
HGNC	HGNC:12335. TRPC3.
HPA	HPA037969.
MIM	602345. gene.
neXtProt	NX_Q13507.
PharmGKB	PA37008.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18516.
HOVERGEN	HBG068337.
InParanoid	Q13507.
OrthoDB	EOG4V436Z.
Enzyme and pathway databases
Pathway_Interaction_DB	pi3kplctrkpathway. Trk receptor signaling mediated by PI3K and PLC-gamma.
Reactome	REACT_111045. Developmental Biology. REACT_111102. Signal Transduction. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q13507.
Bgee	Q13507.
CleanEx	HS_TRPC3.
Genevestigator	Q13507.
GermOnline	ENSG00000138741. Homo sapiens.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans. IPR013555. TRP_2. IPR004729. TRP_channel. IPR005459. TRPC3_channel. IPR002153. TRPC_channel. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
KO	K04966.
Pfam	PF12796. Ank_2. 1 hit. PF00520. Ion_trans. 1 hit. PF08344. TRP_2. 1 hit. [Graphical view]
PRINTS	PR01097. TRNSRECEPTRP. PR01644. TRPCHANNEL3.
SMART	SM00248. ANK. 3 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
TIGRFAMs	TIGR00870. Trp. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	28283.
SOURCE	Search...

Entry information

Entry name

TRPC3_HUMAN

Accession

Primary (citable) accession number: Q13507
Secondary accession number(s): O00593, Q15660, Q52M35

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 3, 2011
Last modified:	January 25, 2012
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents