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Protein

NGFI-A-binding protein 1

Gene

NAB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 1
Alternative name(s):
EGR-1-binding protein 1
Transcriptional regulatory protein p54
Gene namesi
Name:NAB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:7626. NAB1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31431.

Polymorphism and mutation databases

BioMutaiNAB1.
DMDMi90109928.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486NGFI-A-binding protein 1PRO_0000077038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki129 – 129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei172 – 1721PhosphoserineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei328 – 3281PhosphoserineCombined sources
Cross-linki333 – 333Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Curated
Cross-linki333 – 333Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki355 – 355Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei407 – 4071PhosphoserineBy similarity
Cross-linki480 – 480Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Curated
Cross-linki480 – 480Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13506.
MaxQBiQ13506.
PaxDbiQ13506.
PRIDEiQ13506.

PTM databases

iPTMnetiQ13506.
PhosphoSiteiQ13506.

Expressioni

Tissue specificityi

Isoform Short is found in myeloid leukemia cell line KG-1.

Gene expression databases

BgeeiQ13506.
CleanExiHS_NAB1.
ExpressionAtlasiQ13506. baseline and differential.
GenevisibleiQ13506. HS.

Organism-specific databases

HPAiHPA002738.

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.By similarity

Protein-protein interaction databases

BioGridi110746. 15 interactions.
IntActiQ13506. 5 interactions.
STRINGi9606.ENSP00000336894.

Structurei

Secondary structure

1
487
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi193 – 20917Combined sources
Helixi215 – 22410Combined sources
Helixi227 – 2326Combined sources
Helixi234 – 2374Combined sources
Helixi243 – 25210Combined sources
Beta strandi255 – 2573Combined sources
Helixi272 – 28615Combined sources
Helixi288 – 2914Combined sources
Helixi294 – 31017Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YUFNMR-A189-317[»]
ProteinModelPortaliQ13506.
SMRiQ13506. Positions 182-317.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13506.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 8279NCD1Add
BLAST
Regioni221 – 31090NCD2Add
BLAST
Regioni307 – 33832Necessary for nuclear localizationBy similarityAdd
BLAST

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ13506.
OMAiHIFEMSD.
PhylomeDBiQ13506.
TreeFamiTF315501.

Family and domain databases

InterProiIPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamiPF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomiPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q13506-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL
60 70 80 90 100
EIMALVGMAS KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP
110 120 130 140 150
EGSPTWLGIS CSSYERSSNA REPHLKIPKC AATTCVQSLG QGKSDVVGSL
160 170 180 190 200
ALQSVGESRL WQGHHATESE HSLSPADLGS PASPKESSEA LDAAAALSVA
210 220 230 240 250
ECVERMAPTL PKSDLNEVKE LLKTNKKLAK MIGHIFEMND DDPHKEEEIR
260 270 280 290 300
KYSAIYGRFD SKRKDGKHLT LHELTVNEAA AQLCVKDNAL LTRRDELFAL
310 320 330 340 350
ARQISREVTY KYTYRTTKSK CGERDELSPK RIKVEDGFPD FQDSVQTLFQ
360 370 380 390 400
QARAKSEELA ALSSQQPEKV MAKQMEFLCN QAGYERLQHA ERRLSAGLYR
410 420 430 440 450
QSSEEHSPNG LTSDNSDGQG ERPLNLRMPN LQNRQPHHFV VDGELSRLYP
460 470 480
SEAKSHSSES LGILKDYPHS AFTLEKKVIK TEPEDSR
Length:487
Mass (Da):54,401
Last modified:March 7, 2006 - v2
Checksum:i385B16391D670059
GO
Isoform Short (identifier: Q13506-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-364: Missing.

Show »
Length:458
Mass (Da):51,233
Checksum:iC639C90F4EF5657E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291K → N in AAC50588 (PubMed:8668170).Curated
Sequence conflicti366 – 3661Missing in AAC50588 (PubMed:8668170).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei336 – 36429Missing in isoform Short. 1 PublicationVSP_003384Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47007 mRNA. Translation: AAC50588.1.
AF045451 mRNA. Translation: AAC25085.1.
AF045452 mRNA. Translation: AAC25086.1.
AC006460 Genomic DNA. Translation: AAX93076.1.
BC035724 mRNA. Translation: AAH35724.1.
AF052744 Genomic DNA. Translation: AAC28325.1.
CCDSiCCDS2307.1. [Q13506-1]
RefSeqiNP_005957.2. NM_005966.3. [Q13506-1]
XP_005246636.1. XM_005246579.2. [Q13506-1]
XP_005246637.1. XM_005246580.2. [Q13506-1]
XP_005246639.1. XM_005246582.1. [Q13506-1]
XP_005246640.1. XM_005246583.1. [Q13506-1]
XP_011509521.1. XM_011511219.1. [Q13506-1]
UniGeneiHs.107474.

Genome annotation databases

EnsembliENST00000337386; ENSP00000336894; ENSG00000138386. [Q13506-1]
ENST00000409581; ENSP00000387089; ENSG00000138386. [Q13506-1]
GeneIDi4664.
KEGGihsa:4664.
UCSCiuc002usb.4. human. [Q13506-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47007 mRNA. Translation: AAC50588.1.
AF045451 mRNA. Translation: AAC25085.1.
AF045452 mRNA. Translation: AAC25086.1.
AC006460 Genomic DNA. Translation: AAX93076.1.
BC035724 mRNA. Translation: AAH35724.1.
AF052744 Genomic DNA. Translation: AAC28325.1.
CCDSiCCDS2307.1. [Q13506-1]
RefSeqiNP_005957.2. NM_005966.3. [Q13506-1]
XP_005246636.1. XM_005246579.2. [Q13506-1]
XP_005246637.1. XM_005246580.2. [Q13506-1]
XP_005246639.1. XM_005246582.1. [Q13506-1]
XP_005246640.1. XM_005246583.1. [Q13506-1]
XP_011509521.1. XM_011511219.1. [Q13506-1]
UniGeneiHs.107474.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YUFNMR-A189-317[»]
ProteinModelPortaliQ13506.
SMRiQ13506. Positions 182-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110746. 15 interactions.
IntActiQ13506. 5 interactions.
STRINGi9606.ENSP00000336894.

PTM databases

iPTMnetiQ13506.
PhosphoSiteiQ13506.

Polymorphism and mutation databases

BioMutaiNAB1.
DMDMi90109928.

Proteomic databases

EPDiQ13506.
MaxQBiQ13506.
PaxDbiQ13506.
PRIDEiQ13506.

Protocols and materials databases

DNASUi4664.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337386; ENSP00000336894; ENSG00000138386. [Q13506-1]
ENST00000409581; ENSP00000387089; ENSG00000138386. [Q13506-1]
GeneIDi4664.
KEGGihsa:4664.
UCSCiuc002usb.4. human. [Q13506-1]

Organism-specific databases

CTDi4664.
GeneCardsiNAB1.
HGNCiHGNC:7626. NAB1.
HPAiHPA002738.
MIMi600800. gene.
neXtProtiNX_Q13506.
PharmGKBiPA31431.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3835. Eukaryota.
ENOG410YKFH. LUCA.
GeneTreeiENSGT00390000006330.
HOGENOMiHOG000026777.
HOVERGENiHBG003127.
InParanoidiQ13506.
OMAiHIFEMSD.
PhylomeDBiQ13506.
TreeFamiTF315501.

Miscellaneous databases

ChiTaRSiNAB1. human.
EvolutionaryTraceiQ13506.
GeneWikiiNAB1.
GenomeRNAii4664.
PROiQ13506.
SOURCEiSearch...

Gene expression databases

BgeeiQ13506.
CleanExiHS_NAB1.
ExpressionAtlasiQ13506. baseline and differential.
GenevisibleiQ13506. HS.

Family and domain databases

InterProiIPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamiPF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomiPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli."
    Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J.
    Mol. Cell. Biol. 16:3545-3553(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Brain.
  2. Fan W.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  5. "Cloning and characterization of the 5' promoter region of human p54, a transcriptional repressor."
    Cao J., Fan W.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-103.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators."
    Garcia-Gutierrez P., Juarez-Vicente F., Gallardo-Chamizo F., Charnay P., Garcia-Dominguez M.
    EMBO Rep. 12:1018-1023(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION AT LYS-333 AND LYS-480 BY EGR2.
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. "Uncovering global SUMOylation signaling networks in a site-specific manner."
    Hendriks I.A., D'Souza R.C., Yang B., Verlaan-de Vries M., Mann M., Vertegaal A.C.
    Nat. Struct. Mol. Biol. 21:927-936(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-333; LYS-355 AND LYS-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "SUMO-2 orchestrates chromatin modifiers in response to DNA damage."
    Hendriks I.A., Treffers L.W., Verlaan-de Vries M., Olsen J.V., Vertegaal A.C.
    Cell Rep. 10:1778-1791(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-129; LYS-333 AND LYS-480, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "System-wide analysis of SUMOylation dynamics in response to replication stress reveals novel small ubiquitin-like modified target proteins and acceptor lysines relevant for genome stability."
    Xiao Z., Chang J.G., Hendriks I.A., Sigurdsson J.O., Olsen J.V., Vertegaal A.C.
    Mol. Cell. Proteomics 14:1419-1434(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Solution structure of the NCD2 domain in human transcriptional repressor NAB1 protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (APR-2008) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 183-317.

Entry informationi

Entry nameiNAB1_HUMAN
AccessioniPrimary (citable) accession number: Q13506
Secondary accession number(s): O75383
, O75384, Q6GTU1, Q9UEV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 7, 2006
Last modified: June 8, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.