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Q13506 (NAB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NGFI-A-binding protein 1
Alternative name(s):
EGR-1-binding protein 1
Transcriptional regulatory protein p54
Gene names
Name:NAB1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2 By similarity.

Subunit structure

Homomultimers may associate with EGR1 bound to DNA By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Isoform Short is found in myeloid leukemia cell line KG-1.

Domain

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.

The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similarities

Belongs to the NAB family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q13506-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q13506-2)

The sequence of this isoform differs from the canonical sequence as follows:
     336-364: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486NGFI-A-binding protein 1
PRO_0000077038

Regions

Region4 – 8279NCD1
Region221 – 31090NCD2
Region307 – 33832Necessary for nuclear localization By similarity

Amino acid modifications

Modified residue3281Phosphoserine Ref.6
Cross-link333Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable
Cross-link480Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable

Natural variations

Alternative sequence336 – 36429Missing in isoform Short.
VSP_003384

Experimental info

Sequence conflict1291K → N in AAC50588. Ref.1
Sequence conflict3661Missing in AAC50588. Ref.1

Secondary structure

................... 487
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 385B16391D670059

FASTA48754,401
        10         20         30         40         50         60 
MAAALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL EIMALVGMAS 

        70         80         90        100        110        120 
KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA 

       130        140        150        160        170        180 
REPHLKIPKC AATTCVQSLG QGKSDVVGSL ALQSVGESRL WQGHHATESE HSLSPADLGS 

       190        200        210        220        230        240 
PASPKESSEA LDAAAALSVA ECVERMAPTL PKSDLNEVKE LLKTNKKLAK MIGHIFEMND 

       250        260        270        280        290        300 
DDPHKEEEIR KYSAIYGRFD SKRKDGKHLT LHELTVNEAA AQLCVKDNAL LTRRDELFAL 

       310        320        330        340        350        360 
ARQISREVTY KYTYRTTKSK CGERDELSPK RIKVEDGFPD FQDSVQTLFQ QARAKSEELA 

       370        380        390        400        410        420 
ALSSQQPEKV MAKQMEFLCN QAGYERLQHA ERRLSAGLYR QSSEEHSPNG LTSDNSDGQG 

       430        440        450        460        470        480 
ERPLNLRMPN LQNRQPHHFV VDGELSRLYP SEAKSHSSES LGILKDYPHS AFTLEKKVIK 


TEPEDSR 

« Hide

Isoform Short [UniParc].

Checksum: C639C90F4EF5657E
Show »

FASTA45851,233

References

« Hide 'large scale' references
[1]"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli."
Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J.
Mol. Cell. Biol. 16:3545-3553(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Tissue: Brain.
[2]Fan W.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[5]"Cloning and characterization of the 5' promoter region of human p54, a transcriptional repressor."
Cao J., Fan W.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-103.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators."
Garcia-Gutierrez P., Juarez-Vicente F., Gallardo-Chamizo F., Charnay P., Garcia-Dominguez M.
EMBO Rep. 12:1018-1023(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION AT LYS-333 AND LYS-480 BY EGR2.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Solution structure of the NCD2 domain in human transcriptional repressor NAB1 protein."
RIKEN structural genomics initiative (RSGI)
Submitted (APR-2008) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 183-317.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47007 mRNA. Translation: AAC50588.1.
AF045451 mRNA. Translation: AAC25085.1.
AF045452 mRNA. Translation: AAC25086.1.
AC006460 Genomic DNA. Translation: AAX93076.1.
BC035724 mRNA. Translation: AAH35724.1.
AF052744 Genomic DNA. Translation: AAC28325.1.
RefSeqNP_005957.2. NM_005966.3.
XP_005246636.1. XM_005246579.1.
XP_005246637.1. XM_005246580.1.
XP_005246638.1. XM_005246581.1.
XP_005246639.1. XM_005246582.1.
XP_005246640.1. XM_005246583.1.
XP_005246641.1. XM_005246584.1.
XP_005246642.1. XM_005246585.1.
UniGeneHs.107474.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YUFNMR-A189-317[»]
ProteinModelPortalQ13506.
SMRQ13506. Positions 182-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110746. 5 interactions.
IntActQ13506. 1 interaction.
STRING9606.ENSP00000336894.

PTM databases

PhosphoSiteQ13506.

Polymorphism databases

DMDM90109928.

Proteomic databases

PaxDbQ13506.
PRIDEQ13506.

Protocols and materials databases

DNASU4664.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000337386; ENSP00000336894; ENSG00000138386. [Q13506-1]
ENST00000409581; ENSP00000387089; ENSG00000138386. [Q13506-1]
GeneID4664.
KEGGhsa:4664.
UCSCuc002usb.3. human. [Q13506-1]

Organism-specific databases

CTD4664.
GeneCardsGC02P191477.
HGNCHGNC:7626. NAB1.
HPAHPA002738.
MIM600800. gene.
neXtProtNX_Q13506.
PharmGKBPA31431.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307377.
HOGENOMHOG000026777.
HOVERGENHBG003127.
InParanoidQ13506.
OMACTQAGYE.
PhylomeDBQ13506.
TreeFamTF315501.

Gene expression databases

ArrayExpressQ13506.
BgeeQ13506.
CleanExHS_NAB1.
GenevestigatorQ13506.

Family and domain databases

InterProIPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamPF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

ChiTaRSNAB1. human.
EvolutionaryTraceQ13506.
GeneWikiNAB1.
GenomeRNAi4664.
NextBio17974.
PROQ13506.
SOURCESearch...

Entry information

Entry nameNAB1_HUMAN
AccessionPrimary (citable) accession number: Q13506
Secondary accession number(s): O75383 expand/collapse secondary AC list , O75384, Q6GTU1, Q9UEV1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM