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Protein

NGFI-A-binding protein 1

Gene

NAB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NGFI-A-binding protein 1
Alternative name(s):
EGR-1-binding protein 1
Transcriptional regulatory protein p54
Gene namesi
Name:NAB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000138386.16
HGNCiHGNC:7626 NAB1
MIMi600800 gene
neXtProtiNX_Q13506

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4664
OpenTargetsiENSG00000138386
PharmGKBiPA31431

Polymorphism and mutation databases

BioMutaiNAB1
DMDMi90109928

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000770381 – 486NGFI-A-binding protein 1Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki143Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei172PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Cross-linki212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei328PhosphoserineCombined sources1
Cross-linki333Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate1 Publication
Cross-linki333Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki355Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki369Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki373Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei407PhosphoserineBy similarity1
Cross-linki454Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki465Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki480Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate1 Publication
Cross-linki480Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13506
MaxQBiQ13506
PaxDbiQ13506
PeptideAtlasiQ13506
PRIDEiQ13506

PTM databases

iPTMnetiQ13506
PhosphoSitePlusiQ13506

Expressioni

Tissue specificityi

Isoform Short is found in myeloid leukemia cell line KG-1.

Gene expression databases

BgeeiENSG00000138386
CleanExiHS_NAB1
ExpressionAtlasiQ13506 baseline and differential
GenevisibleiQ13506 HS

Organism-specific databases

HPAiHPA002738

Interactioni

Subunit structurei

Homomultimers may associate with EGR1 bound to DNA.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110746, 16 interactors
IntActiQ13506, 5 interactors
STRINGi9606.ENSP00000336894

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi193 – 209Combined sources17
Helixi215 – 224Combined sources10
Helixi227 – 232Combined sources6
Helixi234 – 237Combined sources4
Helixi243 – 252Combined sources10
Beta strandi255 – 257Combined sources3
Helixi272 – 286Combined sources15
Helixi288 – 291Combined sources4
Helixi294 – 310Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YUFNMR-A189-317[»]
ProteinModelPortaliQ13506
SMRiQ13506
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13506

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 82NCD1Add BLAST79
Regioni221 – 310NCD2Add BLAST90
Regioni307 – 338Necessary for nuclear localizationBy similarityAdd BLAST32

Domaini

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Sequence similaritiesi

Belongs to the NAB family.Curated

Phylogenomic databases

eggNOGiKOG3835 Eukaryota
ENOG410YKFH LUCA
GeneTreeiENSGT00390000006330
HOGENOMiHOG000026777
HOVERGENiHBG003127
InParanoidiQ13506
KOiK22388
OMAiAAAQLCM
OrthoDBiEOG091G0NQE
PhylomeDBiQ13506
TreeFamiTF315501

Family and domain databases

Gene3Di3.40.390.60, 1 hit
InterProiView protein in InterPro
IPR006986 Nab1_C
IPR006989 NAB_co-repressor_dom
IPR006988 Nab_N
IPR038398 NCD2_sf
PfamiView protein in Pfam
PF04902 Nab1, 1 hit
PF04904 NCD1, 1 hit
PF04905 NCD2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD342192 Nab_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q13506-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL
60 70 80 90 100
EIMALVGMAS KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP
110 120 130 140 150
EGSPTWLGIS CSSYERSSNA REPHLKIPKC AATTCVQSLG QGKSDVVGSL
160 170 180 190 200
ALQSVGESRL WQGHHATESE HSLSPADLGS PASPKESSEA LDAAAALSVA
210 220 230 240 250
ECVERMAPTL PKSDLNEVKE LLKTNKKLAK MIGHIFEMND DDPHKEEEIR
260 270 280 290 300
KYSAIYGRFD SKRKDGKHLT LHELTVNEAA AQLCVKDNAL LTRRDELFAL
310 320 330 340 350
ARQISREVTY KYTYRTTKSK CGERDELSPK RIKVEDGFPD FQDSVQTLFQ
360 370 380 390 400
QARAKSEELA ALSSQQPEKV MAKQMEFLCN QAGYERLQHA ERRLSAGLYR
410 420 430 440 450
QSSEEHSPNG LTSDNSDGQG ERPLNLRMPN LQNRQPHHFV VDGELSRLYP
460 470 480
SEAKSHSSES LGILKDYPHS AFTLEKKVIK TEPEDSR
Length:487
Mass (Da):54,401
Last modified:March 7, 2006 - v2
Checksum:i385B16391D670059
GO
Isoform Short (identifier: Q13506-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-364: Missing.

Show »
Length:458
Mass (Da):51,233
Checksum:iC639C90F4EF5657E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129K → N in AAC50588 (PubMed:8668170).Curated1
Sequence conflicti366Missing in AAC50588 (PubMed:8668170).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003384336 – 364Missing in isoform Short. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47007 mRNA Translation: AAC50588.1
AF045451 mRNA Translation: AAC25085.1
AF045452 mRNA Translation: AAC25086.1
AC006460 Genomic DNA Translation: AAX93076.1
BC035724 mRNA Translation: AAH35724.1
AF052744 Genomic DNA Translation: AAC28325.1
CCDSiCCDS2307.1 [Q13506-1]
RefSeqiNP_001308241.1, NM_001321312.1 [Q13506-1]
NP_001308242.1, NM_001321313.1 [Q13506-1]
NP_001308243.1, NM_001321314.1
NP_001308244.1, NM_001321315.1
NP_005957.2, NM_005966.3 [Q13506-1]
XP_005246639.1, XM_005246582.1 [Q13506-1]
XP_005246640.1, XM_005246583.1 [Q13506-1]
XP_011509521.1, XM_011511219.2 [Q13506-1]
XP_016859659.1, XM_017004170.1 [Q13506-1]
XP_016859665.1, XM_017004176.1 [Q13506-1]
UniGeneiHs.107474

Genome annotation databases

EnsembliENST00000337386; ENSP00000336894; ENSG00000138386 [Q13506-1]
ENST00000409581; ENSP00000387089; ENSG00000138386 [Q13506-1]
GeneIDi4664
KEGGihsa:4664
UCSCiuc002usb.4 human [Q13506-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNAB1_HUMAN
AccessioniPrimary (citable) accession number: Q13506
Secondary accession number(s): O75383
, O75384, Q6GTU1, Q9UEV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health