Q13506 (NAB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NGFI-A-binding protein 1

Alternative name(s):
EGR-1-binding protein 1
Transcriptional regulatory protein p54

Gene names

Name:

NAB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2 By similarity.
Subunit structure	Homomultimers may associate with EGR1 bound to DNA By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Isoform Short is found in a myeloid leukemia cell line.
Domain	The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization. The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Sequence similarities	Belongs to the NAB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Non-traceable author statement Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q13506-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q13506-2)

The sequence of this isoform differs from the canonical sequence as follows:
     336-364: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 486

486

NGFI-A-binding protein 1

PRO_0000077038

Regions

Region

4 – 82

79

NCD1

Region

221 – 310

90

NCD2

Region

307 – 338

32

Necessary for nuclear localization By similarity

Amino acid modifications

Modified residue

328

1

Phosphoserine Ref.6

Modified residue

403

1

Phosphoserine By similarity

Cross-link

333

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable

Cross-link

480

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable

Natural variations

Alternative sequence

336 – 364

29

Missing in isoform Short.

VSP_003384

Experimental info

Sequence conflict

129

1

K → N in AAC50588. Ref.1

Sequence conflict

366

1

Missing in AAC50588. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

487

Helix Strand Turn

Details...

Helix

193 – 209

17

Helix

215 – 224

10

Helix

227 – 231

5

Turn

235 – 237

3

Helix

243 – 252

10

Helix

272 – 286

15

Helix

288 – 291

4

Helix

294 – 310

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 385B16391D670059	FASTA	487	54,401
	10 20 30 40 50 60 MAAALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL EIMALVGMAS 70 80 90 100 110 120 KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA 130 140 150 160 170 180 REPHLKIPKC AATTCVQSLG QGKSDVVGSL ALQSVGESRL WQGHHATESE HSLSPADLGS 190 200 210 220 230 240 PASPKESSEA LDAAAALSVA ECVERMAPTL PKSDLNEVKE LLKTNKKLAK MIGHIFEMND 250 260 270 280 290 300 DDPHKEEEIR KYSAIYGRFD SKRKDGKHLT LHELTVNEAA AQLCVKDNAL LTRRDELFAL 310 320 330 340 350 360 ARQISREVTY KYTYRTTKSK CGERDELSPK RIKVEDGFPD FQDSVQTLFQ QARAKSEELA 370 380 390 400 410 420 ALSSQQPEKV MAKQMEFLCN QAGYERLQHA ERRLSAGLYR QSSEEHSPNG LTSDNSDGQG 430 440 450 460 470 480 ERPLNLRMPN LQNRQPHHFV VDGELSRLYP SEAKSHSSES LGILKDYPHS AFTLEKKVIK TEPEDSR
	Isoform Short [UniParc]. Checksum: C639C90F4EF5657E	FASTA	458	51,233
	10 20 30 40 50 60 MAAALPRTLG ELQLYRILQK ANLLSYFDAF IQQGGDDVQQ LCEAGEEEFL EIMALVGMAS 70 80 90 100 110 120 KPLHVRRLQK ALRDWVTNPG LFNQPLTSLP VSSIPIYKLP EGSPTWLGIS CSSYERSSNA 130 140 150 160 170 180 REPHLKIPKC AATTCVQSLG QGKSDVVGSL ALQSVGESRL WQGHHATESE HSLSPADLGS 190 200 210 220 230 240 PASPKESSEA LDAAAALSVA ECVERMAPTL PKSDLNEVKE LLKTNKKLAK MIGHIFEMND 250 260 270 280 290 300 DDPHKEEEIR KYSAIYGRFD SKRKDGKHLT LHELTVNEAA AQLCVKDNAL LTRRDELFAL 310 320 330 340 350 360 ARQISREVTY KYTYRTTKSK CGERDELSPK RIKVEQQPEK VMAKQMEFLC NQAGYERLQH 370 380 390 400 410 420 AERRLSAGLY RQSSEEHSPN GLTSDNSDGQ GERPLNLRMP NLQNRQPHHF VVDGELSRLY 430 440 450 PSEAKSHSSE SLGILKDYPH SAFTLEKKVI KTEPEDSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli." Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J. Mol. Cell. Biol. 16:3545-3553(1996) [PubMed: 8668170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Tissue: Brain.
[2]	Fan W. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[5]	"Cloning and characterization of the 5' promoter region of human p54, a transcriptional repressor." Cao J., Fan W. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-103.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators." Garcia-Gutierrez P., Juarez-Vicente F., Gallardo-Chamizo F., Charnay P., Garcia-Dominguez M. EMBO Rep. 12:1018-1023(2011) [PubMed: 21836637] [Abstract] Cited for: SUMOYLATION AT LYS-333 AND LYS-480 BY EGR2.
[8]	"Solution structure of the NCD2 domain in human transcriptional repressor NAB1 protein." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 183-317.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U47007 mRNA. Translation: AAC50588.1.
AF045451 mRNA. Translation: AAC25085.1.
AF045452 mRNA. Translation: AAC25086.1.
AC006460 Genomic DNA. Translation: AAX93076.1.
BC035724 mRNA. Translation: AAH35724.1.
AF052744 Genomic DNA. Translation: AAC28325.1.

IPI

IPI00220133.
IPI00336119.

RefSeq

NP_005957.2. NM_005966.3.

UniGene

Hs.107474.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YUF	NMR	-	A	189-317	[»]

ProteinModelPortal

Q13506.

SMR

Q13506. Positions 182-317.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13506. 1 interaction.

STRING

Q13506.

PTM databases

PhosphoSite

Q13506.

Polymorphism databases

DMDM

90109928.

Proteomic databases

PRIDE

Q13506.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000337386; ENSP00000336894; ENSG00000138386.
ENST00000409581; ENSP00000387089; ENSG00000138386.

GeneID

4664.

KEGG

hsa:4664.

UCSC

uc002usb.1. human.

Organism-specific databases

CTD

4664.

GeneCards

GC02P191477.

H-InvDB

HIX0023916.

HGNC

HGNC:7626. NAB1.

HPA

HPA002738.

MIM

600800. gene.

neXtProt

NX_Q13506.

PharmGKB

PA31431.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09029.

GeneTree

ENSGT00390000006330.

HOGENOM

HBG447154.

HOVERGEN

HBG003127.

InParanoid

Q13506.

OMA

HHTTESE.

OrthoDB

EOG4VX254.

PhylomeDB

Q13506.

Gene expression databases

ArrayExpress

Q13506.

Bgee

Q13506.

CleanEx

HS_NAB1.

Genevestigator

Q13506.

GermOnline

ENSG00000138386. Homo sapiens.

Family and domain databases

InterPro

IPR006986. Nab1_C.
IPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]

Pfam

PF04902. Nab1. 1 hit.
PF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]

ProDom

PD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

NextBio

17974.

SOURCE

Search...

Entry information

Entry name

NAB1_HUMAN

Accession

Primary (citable) accession number: Q13506
Secondary accession number(s): O75383 , O75384, Q6GTU1, Q9UEV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.