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Protein

Mediator of RNA polymerase II transcription subunit 21

Gene

MED21

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  • DNA-directed RNA polymerase activity Source: ProtInc
  • RNA polymerase II transcription cofactor activity Source: GO_Central
  • transcription coactivator activity Source: MGI
  • ubiquitin protein ligase activity Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 21
Alternative name(s):
Mediator complex subunit 21
RNA polymerase II holoenzyme component SRB7
Short name:
RNAPII complex component SRB7
Short name:
hSrb7
Gene namesi
Name:MED21
Synonyms:SRB7, SURB7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:11473. MED21.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395485.

Polymorphism and mutation databases

DMDMi7531204.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Mediator of RNA polymerase II transcription subunit 21PRO_0000096375Add
BLAST

Proteomic databases

EPDiQ13503.
MaxQBiQ13503.
PaxDbiQ13503.
PeptideAtlasiQ13503.
PRIDEiQ13503.
TopDownProteomicsiQ13503.

PTM databases

iPTMnetiQ13503.
PhosphoSiteiQ13503.

Expressioni

Gene expression databases

BgeeiENSG00000152944.
CleanExiHS_MED21.
ExpressionAtlasiQ13503. baseline and differential.
GenevisibleiQ13503. HS.

Organism-specific databases

HPAiHPA017177.

Interactioni

Subunit structurei

Interacts with PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BLOC1S6Q9UL453EBI-394678,EBI-465781
MAGEB4O154813EBI-394678,EBI-751857
MED9Q9NWA03EBI-394678,EBI-394653
SSC5DA1L4H13EBI-394678,EBI-10172867
ZNF655Q8N7203EBI-394678,EBI-625509

Protein-protein interaction databases

BioGridi114807. 115 interactions.
DIPiDIP-31471N.
IntActiQ13503. 33 interactions.
MINTiMINT-5008842.
STRINGi9606.ENSP00000282892.

Structurei

3D structure databases

ProteinModelPortaliQ13503.
SMRiQ13503. Positions 3-129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 21 family.Curated

Phylogenomic databases

eggNOGiKOG1510. Eukaryota.
ENOG4111I75. LUCA.
GeneTreeiENSGT00390000014557.
HOGENOMiHOG000006767.
HOVERGENiHBG009229.
InParanoidiQ13503.
KOiK15152.
PhylomeDBiQ13503.

Family and domain databases

InterProiIPR021384. Mediator_Med21.
[Graphical view]
PfamiPF11221. Med21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP
60 70 80 90 100
TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA
110 120 130 140
ATCLEDVVYR GDMLLEKIQS ALADIAQSQL KTRSGTHSQS LPDS
Length:144
Mass (Da):15,564
Last modified:November 1, 1996 - v1
Checksum:iF786169620EDC126
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041L → V in AAB06944 (PubMed:8609996).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46837 mRNA. Translation: AAA98511.1.
U52960 mRNA. Translation: AAB06944.1.
CR456999 mRNA. Translation: CAG33280.1.
AK311838 mRNA. Translation: BAG34780.1.
CH471094 Genomic DNA. Translation: EAW96545.1.
BC008380 mRNA. Translation: AAH08380.1.
CCDSiCCDS8711.1.
PIRiS68454.
RefSeqiNP_004255.2. NM_004264.4.
UniGeneiHs.286145.

Genome annotation databases

EnsembliENST00000282892; ENSP00000282892; ENSG00000152944.
GeneIDi9412.
KEGGihsa:9412.
UCSCiuc001rhp.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46837 mRNA. Translation: AAA98511.1.
U52960 mRNA. Translation: AAB06944.1.
CR456999 mRNA. Translation: CAG33280.1.
AK311838 mRNA. Translation: BAG34780.1.
CH471094 Genomic DNA. Translation: EAW96545.1.
BC008380 mRNA. Translation: AAH08380.1.
CCDSiCCDS8711.1.
PIRiS68454.
RefSeqiNP_004255.2. NM_004264.4.
UniGeneiHs.286145.

3D structure databases

ProteinModelPortaliQ13503.
SMRiQ13503. Positions 3-129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114807. 115 interactions.
DIPiDIP-31471N.
IntActiQ13503. 33 interactions.
MINTiMINT-5008842.
STRINGi9606.ENSP00000282892.

PTM databases

iPTMnetiQ13503.
PhosphoSiteiQ13503.

Polymorphism and mutation databases

DMDMi7531204.

Proteomic databases

EPDiQ13503.
MaxQBiQ13503.
PaxDbiQ13503.
PeptideAtlasiQ13503.
PRIDEiQ13503.
TopDownProteomicsiQ13503.

Protocols and materials databases

DNASUi9412.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282892; ENSP00000282892; ENSG00000152944.
GeneIDi9412.
KEGGihsa:9412.
UCSCiuc001rhp.3. human.

Organism-specific databases

CTDi9412.
GeneCardsiMED21.
HGNCiHGNC:11473. MED21.
HPAiHPA017177.
MIMi603800. gene.
neXtProtiNX_Q13503.
PharmGKBiPA162395485.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1510. Eukaryota.
ENOG4111I75. LUCA.
GeneTreeiENSGT00390000014557.
HOGENOMiHOG000006767.
HOVERGENiHBG009229.
InParanoidiQ13503.
KOiK15152.
PhylomeDBiQ13503.

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED21. human.
GeneWikiiMED21.
GenomeRNAii9412.
PROiQ13503.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000152944.
CleanExiHS_MED21.
ExpressionAtlasiQ13503. baseline and differential.
GenevisibleiQ13503. HS.

Family and domain databases

InterProiIPR021384. Mediator_Med21.
[Graphical view]
PfamiPF11221. Med21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED21_HUMAN
AccessioniPrimary (citable) accession number: Q13503
Secondary accession number(s): B2R4I3, Q6IB05, Q92811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.