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Q13503 (MED21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 21
Alternative name(s):
Mediator complex subunit 21
RNA polymerase II holoenzyme component SRB7
Short name=RNAPII complex component SRB7
Short name=hSrb7
Gene names
Name:MED21
Synonyms:SRB7, SURB7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.8 Ref.14

Subunit structure

Interacts with PPARG By similarity. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion. Ref.1 Ref.2 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the Mediator complex subunit 21 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 144144Mediator of RNA polymerase II transcription subunit 21
PRO_0000096375

Experimental info

Sequence conflict1041L → V in AAB06944. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q13503 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F786169620EDC126

FASTA14415,564
        10         20         30         40         50         60 
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA 

        70         80         90        100        110        120 
LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA ATCLEDVVYR GDMLLEKIQS 

       130        140 
ALADIAQSQL KTRSGTHSQS LPDS 

« Hide

References

« Hide 'large scale' references
[1]"A mammalian SRB protein associated with an RNA polymerase II holoenzyme."
Chao D.M., Gadbois E.L., Murray P.J., Anderson S.F., Sonu M.S., Parvin J.D., Young R.A.
Nature 380:82-85(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
[2]"A human RNA polymerase II complex associated with SRB and DNA-repair proteins."
Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C.W., Linn S., Reinberg D.
Nature 381:86-89(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
Tissue: Liver.
[3]Erratum
Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C.W., Linn S., Reinberg D.
Nature 384:384-384(1996)
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[8]"Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs."
Oelgeschlaeger T., Tao Y., Kang Y.K., Roeder R.G.
Mol. Cell 1:925-931(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription."
Sun X., Zhang Y., Cho H., Rickert P., Lees E., Lane W.S., Reinberg D.
Mol. Cell 2:213-222(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A FORM OF THE MEDIATOR COMPLEX.
[10]"The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex."
Xiao H., Tao Y., Roeder R.G.
J. Biol. Chem. 274:3937-3940(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNA POLYMERASE II AND MED21.
[11]"A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."
Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G.
Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMCC COMPLEX.
[12]Erratum
Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G.
Mol. Cell 3:541-541(1999)
[13]"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[14]"hSrb7, an essential human Mediator component, acts as a coactivator for the thyroid hormone receptor."
Nevado J., Tenbaum S.P., Aranda A.
Mol. Cell. Endocrinol. 222:41-51(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THRA.
[15]"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED18; MED20; MED24; MED28 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46837 mRNA. Translation: AAA98511.1.
U52960 mRNA. Translation: AAB06944.1.
CR456999 mRNA. Translation: CAG33280.1.
AK311838 mRNA. Translation: BAG34780.1.
CH471094 Genomic DNA. Translation: EAW96545.1.
BC008380 mRNA. Translation: AAH08380.1.
PIRS68454.
RefSeqNP_004255.2. NM_004264.4.
UniGeneHs.286145.

3D structure databases

ProteinModelPortalQ13503.
SMRQ13503. Positions 3-129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114807. 52 interactions.
DIPDIP-31471N.
IntActQ13503. 20 interactions.
MINTMINT-5008842.
STRING9606.ENSP00000282892.

PTM databases

PhosphoSiteQ13503.

Polymorphism databases

DMDM7531204.

Proteomic databases

PaxDbQ13503.
PeptideAtlasQ13503.
PRIDEQ13503.

Protocols and materials databases

DNASU9412.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000282892; ENSP00000282892; ENSG00000152944.
GeneID9412.
KEGGhsa:9412.
UCSCuc001rhp.2. human.

Organism-specific databases

CTD9412.
GeneCardsGC12P027175.
HGNCHGNC:11473. MED21.
HPAHPA017177.
MIM603800. gene.
neXtProtNX_Q13503.
PharmGKBPA162395485.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG247021.
HOGENOMHOG000006767.
HOVERGENHBG009229.
InParanoidQ13503.
KOK15152.
PhylomeDBQ13503.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ13503.
BgeeQ13503.
CleanExHS_MED21.
GenevestigatorQ13503.

Family and domain databases

InterProIPR021384. Mediator_Med21.
[Graphical view]
PfamPF11221. Med21. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMED21.
GenomeRNAi9412.
NextBio35260.
PROQ13503.
SOURCESearch...

Entry information

Entry nameMED21_HUMAN
AccessionPrimary (citable) accession number: Q13503
Secondary accession number(s): B2R4I3, Q6IB05, Q92811
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM