Q13503 (MED21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 21

Alternative name(s):
Mediator complex subunit 21
RNA polymerase II holoenzyme component SRB7
Short name=RNAPII complex component SRB7
Short name=hSrb7

Gene names

Name:	MED21
Synonyms:	SRB7, SURB7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.8 Ref.14
Subunit structure	Interacts with PPARG By similarity. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion. Ref.1 Ref.2 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Mediator complex subunit 21 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blastocyst development Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 12037571. Source: MGI stem cell maintenance Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mediator complex Inferred from direct assay PubMed 12037571. Source: MGI
Molecular_function	DNA-directed RNA polymerase activity Traceable author statement Ref.1. Source: ProtInc transcription coactivator activity Inferred from direct assay PubMed 12037571. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Mediator of RNA polymerase II transcription subunit 21		PRO_0000096375
Experimental info
	Sequence conflict	104	1	L → V in AAB06944. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q13503 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F786169620EDC126	FASTA	144	15,564
	10 20 30 40 50 60 MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA 70 80 90 100 110 120 LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA ATCLEDVVYR GDMLLEKIQS 130 140 ALADIAQSQL KTRSGTHSQS LPDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mammalian SRB protein associated with an RNA polymerase II holoenzyme." Chao D.M., Gadbois E.L., Murray P.J., Anderson S.F., Sonu M.S., Parvin J.D., Young R.A. Nature 380:82-85(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
[2]	"A human RNA polymerase II complex associated with SRB and DNA-repair proteins." Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C.W., Linn S., Reinberg D. Nature 381:86-89(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II. Tissue: Liver.
[3]	Erratum Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C.W., Linn S., Reinberg D. Nature 384:384-384(1996)
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[8]	"Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs." Oelgeschlaeger T., Tao Y., Kang Y.K., Roeder R.G. Mol. Cell 1:925-931(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription." Sun X., Zhang Y., Cho H., Rickert P., Lees E., Lane W.S., Reinberg D. Mol. Cell 2:213-222(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A FORM OF THE MEDIATOR COMPLEX.
[10]	"The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex." Xiao H., Tao Y., Roeder R.G. J. Biol. Chem. 274:3937-3940(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RNA POLYMERASE II AND MED21.
[11]	"A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation." Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G. Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMCC COMPLEX.
[12]	Erratum Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G. Mol. Cell 3:541-541(1999)
[13]	"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C. Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[14]	"hSrb7, an essential human Mediator component, acts as a coactivator for the thyroid hormone receptor." Nevado J., Tenbaum S.P., Aranda A. Mol. Cell. Endocrinol. 222:41-51(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THRA.
[15]	"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G. Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED18; MED20; MED24; MED28 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46837 mRNA. Translation: AAA98511.1. U52960 mRNA. Translation: AAB06944.1. CR456999 mRNA. Translation: CAG33280.1. AK311838 mRNA. Translation: BAG34780.1. CH471094 Genomic DNA. Translation: EAW96545.1. BC008380 mRNA. Translation: AAH08380.1.
IPI	IPI00013677.
PIR	S68454.
RefSeq	NP_004255.2. NM_004264.4.
UniGene	Hs.286145.
3D structure databases
ProteinModelPortal	Q13503.
SMR	Q13503. Positions 3-125.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31471N.
IntAct	Q13503. 19 interactions.
STRING	9606.ENSP00000282892.
PTM databases
PhosphoSite	Q13503.
Polymorphism databases
DMDM	7531204.
Proteomic databases
PaxDb	Q13503.
PeptideAtlas	Q13503.
PRIDE	Q13503.
Protocols and materials databases
DNASU	9412.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282892; ENSP00000282892; ENSG00000152944.
GeneID	9412.
KEGG	hsa:9412.
UCSC	uc001rhp.1. human.
Organism-specific databases
CTD	9412.
GeneCards	GC12P027175.
HGNC	HGNC:11473. MED21.
HPA	HPA017177.
MIM	603800. gene.
neXtProt	NX_Q13503.
PharmGKB	PA162395485.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG247021.
HOGENOM	HOG000006767.
HOVERGEN	HBG009229.
InParanoid	Q13503.
KO	K15152.
OrthoDB	EOG4TF0MJ.
PhylomeDB	Q13503.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology.
Gene expression databases
ArrayExpress	Q13503.
Bgee	Q13503.
CleanEx	HS_MED21.
Genevestigator	Q13503.
GermOnline	ENSG00000152944. Homo sapiens.
Family and domain databases
InterPro	IPR021384. Mediator_Med21. [Graphical view]
Pfam	PF11221. Med21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9412.
NextBio	35260.
SOURCE	Search...

Entry information

Entry name

MED21_HUMAN

Accession

Primary (citable) accession number: Q13503
Secondary accession number(s): B2R4I3, Q6IB05, Q92811

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.