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Protein

Mediator of RNA polymerase II transcription subunit 21

Gene

MED21

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  1. DNA-directed RNA polymerase activity Source: ProtInc
  2. transcription coactivator activity Source: MGI

GO - Biological processi

  1. blastocyst development Source: Ensembl
  2. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  3. regulation of transcription from RNA polymerase II promoter Source: ProtInc
  4. stem cell maintenance Source: Ensembl
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 21
Alternative name(s):
Mediator complex subunit 21
RNA polymerase II holoenzyme component SRB7
Short name:
RNAPII complex component SRB7
Short name:
hSrb7
Gene namesi
Name:MED21
Synonyms:SRB7, SURB7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:11473. MED21.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395485.

Polymorphism and mutation databases

DMDMi7531204.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Mediator of RNA polymerase II transcription subunit 21PRO_0000096375Add
BLAST

Proteomic databases

MaxQBiQ13503.
PaxDbiQ13503.
PeptideAtlasiQ13503.
PRIDEiQ13503.

PTM databases

PhosphoSiteiQ13503.

Expressioni

Gene expression databases

BgeeiQ13503.
CleanExiHS_MED21.
ExpressionAtlasiQ13503. baseline and differential.
GenevestigatoriQ13503.

Organism-specific databases

HPAiHPA017177.

Interactioni

Subunit structurei

Interacts with PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BLOC1S6Q9UL453EBI-394678,EBI-465781
MAGEB4O154813EBI-394678,EBI-751857
MED9Q9NWA03EBI-394678,EBI-394653
SSC5DA1L4H13EBI-394678,EBI-10172867
ZNF655Q8N7203EBI-394678,EBI-625509

Protein-protein interaction databases

BioGridi114807. 59 interactions.
DIPiDIP-31471N.
IntActiQ13503. 27 interactions.
MINTiMINT-5008842.
STRINGi9606.ENSP00000282892.

Structurei

3D structure databases

ProteinModelPortaliQ13503.
SMRiQ13503. Positions 3-129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 21 family.Curated

Phylogenomic databases

eggNOGiNOG247021.
GeneTreeiENSGT00390000014557.
HOGENOMiHOG000006767.
HOVERGENiHBG009229.
InParanoidiQ13503.
KOiK15152.
PhylomeDBiQ13503.

Family and domain databases

InterProiIPR021384. Mediator_Med21.
[Graphical view]
PfamiPF11221. Med21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP
60 70 80 90 100
TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA
110 120 130 140
ATCLEDVVYR GDMLLEKIQS ALADIAQSQL KTRSGTHSQS LPDS
Length:144
Mass (Da):15,564
Last modified:November 1, 1996 - v1
Checksum:iF786169620EDC126
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041L → V in AAB06944 (PubMed:8609996).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46837 mRNA. Translation: AAA98511.1.
U52960 mRNA. Translation: AAB06944.1.
CR456999 mRNA. Translation: CAG33280.1.
AK311838 mRNA. Translation: BAG34780.1.
CH471094 Genomic DNA. Translation: EAW96545.1.
BC008380 mRNA. Translation: AAH08380.1.
CCDSiCCDS8711.1.
PIRiS68454.
RefSeqiNP_004255.2. NM_004264.4.
UniGeneiHs.286145.

Genome annotation databases

EnsembliENST00000282892; ENSP00000282892; ENSG00000152944.
GeneIDi9412.
KEGGihsa:9412.
UCSCiuc001rhp.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46837 mRNA. Translation: AAA98511.1.
U52960 mRNA. Translation: AAB06944.1.
CR456999 mRNA. Translation: CAG33280.1.
AK311838 mRNA. Translation: BAG34780.1.
CH471094 Genomic DNA. Translation: EAW96545.1.
BC008380 mRNA. Translation: AAH08380.1.
CCDSiCCDS8711.1.
PIRiS68454.
RefSeqiNP_004255.2. NM_004264.4.
UniGeneiHs.286145.

3D structure databases

ProteinModelPortaliQ13503.
SMRiQ13503. Positions 3-129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114807. 59 interactions.
DIPiDIP-31471N.
IntActiQ13503. 27 interactions.
MINTiMINT-5008842.
STRINGi9606.ENSP00000282892.

PTM databases

PhosphoSiteiQ13503.

Polymorphism and mutation databases

DMDMi7531204.

Proteomic databases

MaxQBiQ13503.
PaxDbiQ13503.
PeptideAtlasiQ13503.
PRIDEiQ13503.

Protocols and materials databases

DNASUi9412.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282892; ENSP00000282892; ENSG00000152944.
GeneIDi9412.
KEGGihsa:9412.
UCSCiuc001rhp.2. human.

Organism-specific databases

CTDi9412.
GeneCardsiGC12P027175.
HGNCiHGNC:11473. MED21.
HPAiHPA017177.
MIMi603800. gene.
neXtProtiNX_Q13503.
PharmGKBiPA162395485.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG247021.
GeneTreeiENSGT00390000014557.
HOGENOMiHOG000006767.
HOVERGENiHBG009229.
InParanoidiQ13503.
KOiK15152.
PhylomeDBiQ13503.

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED21. human.
GeneWikiiMED21.
GenomeRNAii9412.
NextBioi35260.
PROiQ13503.
SOURCEiSearch...

Gene expression databases

BgeeiQ13503.
CleanExiHS_MED21.
ExpressionAtlasiQ13503. baseline and differential.
GenevestigatoriQ13503.

Family and domain databases

InterProiIPR021384. Mediator_Med21.
[Graphical view]
PfamiPF11221. Med21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mammalian SRB protein associated with an RNA polymerase II holoenzyme."
    Chao D.M., Gadbois E.L., Murray P.J., Anderson S.F., Sonu M.S., Parvin J.D., Young R.A.
    Nature 380:82-85(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
  2. "A human RNA polymerase II complex associated with SRB and DNA-repair proteins."
    Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C.W., Linn S., Reinberg D.
    Nature 381:86-89(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
    Tissue: Liver.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  7. "Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs."
    Oelgeschlaeger T., Tao Y., Kang Y.K., Roeder R.G.
    Mol. Cell 1:925-931(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription."
    Sun X., Zhang Y., Cho H., Rickert P., Lees E., Lane W.S., Reinberg D.
    Mol. Cell 2:213-222(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A FORM OF THE MEDIATOR COMPLEX.
  9. "The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex."
    Xiao H., Tao Y., Roeder R.G.
    J. Biol. Chem. 274:3937-3940(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNA POLYMERASE II AND MED21.
  10. "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."
    Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G.
    Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMCC COMPLEX.
  11. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  12. "hSrb7, an essential human Mediator component, acts as a coactivator for the thyroid hormone receptor."
    Nevado J., Tenbaum S.P., Aranda A.
    Mol. Cell. Endocrinol. 222:41-51(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THRA.
  13. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED18; MED20; MED24; MED28 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED21_HUMAN
AccessioniPrimary (citable) accession number: Q13503
Secondary accession number(s): B2R4I3, Q6IB05, Q92811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.