Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q13503

- MED21_HUMAN

UniProt

Q13503 - MED21_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 21

Gene

MED21

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

    GO - Molecular functioni

    1. DNA-directed RNA polymerase activity Source: ProtInc
    2. transcription coactivator activity Source: MGI

    GO - Biological processi

    1. blastocyst development Source: Ensembl
    2. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    3. regulation of transcription from RNA polymerase II promoter Source: ProtInc
    4. stem cell maintenance Source: Ensembl
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 21
    Alternative name(s):
    Mediator complex subunit 21
    RNA polymerase II holoenzyme component SRB7
    Short name:
    RNAPII complex component SRB7
    Short name:
    hSrb7
    Gene namesi
    Name:MED21
    Synonyms:SRB7, SURB7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:11473. MED21.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395485.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 144144Mediator of RNA polymerase II transcription subunit 21PRO_0000096375Add
    BLAST

    Proteomic databases

    MaxQBiQ13503.
    PaxDbiQ13503.
    PeptideAtlasiQ13503.
    PRIDEiQ13503.

    PTM databases

    PhosphoSiteiQ13503.

    Expressioni

    Gene expression databases

    ArrayExpressiQ13503.
    BgeeiQ13503.
    CleanExiHS_MED21.
    GenevestigatoriQ13503.

    Organism-specific databases

    HPAiHPA017177.

    Interactioni

    Subunit structurei

    Interacts with PPARG By similarity. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion.By similarity8 Publications

    Protein-protein interaction databases

    BioGridi114807. 52 interactions.
    DIPiDIP-31471N.
    IntActiQ13503. 20 interactions.
    MINTiMINT-5008842.
    STRINGi9606.ENSP00000282892.

    Structurei

    3D structure databases

    ProteinModelPortaliQ13503.
    SMRiQ13503. Positions 3-129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 21 family.Curated

    Phylogenomic databases

    eggNOGiNOG247021.
    HOGENOMiHOG000006767.
    HOVERGENiHBG009229.
    InParanoidiQ13503.
    KOiK15152.
    PhylomeDBiQ13503.

    Family and domain databases

    InterProiIPR021384. Mediator_Med21.
    [Graphical view]
    PfamiPF11221. Med21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q13503-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP    50
    TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA 100
    ATCLEDVVYR GDMLLEKIQS ALADIAQSQL KTRSGTHSQS LPDS 144
    Length:144
    Mass (Da):15,564
    Last modified:November 1, 1996 - v1
    Checksum:iF786169620EDC126
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti104 – 1041L → V in AAB06944. (PubMed:8609996)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46837 mRNA. Translation: AAA98511.1.
    U52960 mRNA. Translation: AAB06944.1.
    CR456999 mRNA. Translation: CAG33280.1.
    AK311838 mRNA. Translation: BAG34780.1.
    CH471094 Genomic DNA. Translation: EAW96545.1.
    BC008380 mRNA. Translation: AAH08380.1.
    CCDSiCCDS8711.1.
    PIRiS68454.
    RefSeqiNP_004255.2. NM_004264.4.
    UniGeneiHs.286145.

    Genome annotation databases

    EnsembliENST00000282892; ENSP00000282892; ENSG00000152944.
    GeneIDi9412.
    KEGGihsa:9412.
    UCSCiuc001rhp.2. human.

    Polymorphism databases

    DMDMi7531204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46837 mRNA. Translation: AAA98511.1 .
    U52960 mRNA. Translation: AAB06944.1 .
    CR456999 mRNA. Translation: CAG33280.1 .
    AK311838 mRNA. Translation: BAG34780.1 .
    CH471094 Genomic DNA. Translation: EAW96545.1 .
    BC008380 mRNA. Translation: AAH08380.1 .
    CCDSi CCDS8711.1.
    PIRi S68454.
    RefSeqi NP_004255.2. NM_004264.4.
    UniGenei Hs.286145.

    3D structure databases

    ProteinModelPortali Q13503.
    SMRi Q13503. Positions 3-129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114807. 52 interactions.
    DIPi DIP-31471N.
    IntActi Q13503. 20 interactions.
    MINTi MINT-5008842.
    STRINGi 9606.ENSP00000282892.

    PTM databases

    PhosphoSitei Q13503.

    Polymorphism databases

    DMDMi 7531204.

    Proteomic databases

    MaxQBi Q13503.
    PaxDbi Q13503.
    PeptideAtlasi Q13503.
    PRIDEi Q13503.

    Protocols and materials databases

    DNASUi 9412.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000282892 ; ENSP00000282892 ; ENSG00000152944 .
    GeneIDi 9412.
    KEGGi hsa:9412.
    UCSCi uc001rhp.2. human.

    Organism-specific databases

    CTDi 9412.
    GeneCardsi GC12P027175.
    HGNCi HGNC:11473. MED21.
    HPAi HPA017177.
    MIMi 603800. gene.
    neXtProti NX_Q13503.
    PharmGKBi PA162395485.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG247021.
    HOGENOMi HOG000006767.
    HOVERGENi HBG009229.
    InParanoidi Q13503.
    KOi K15152.
    PhylomeDBi Q13503.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GeneWikii MED21.
    GenomeRNAii 9412.
    NextBioi 35260.
    PROi Q13503.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13503.
    Bgeei Q13503.
    CleanExi HS_MED21.
    Genevestigatori Q13503.

    Family and domain databases

    InterProi IPR021384. Mediator_Med21.
    [Graphical view ]
    Pfami PF11221. Med21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A mammalian SRB protein associated with an RNA polymerase II holoenzyme."
      Chao D.M., Gadbois E.L., Murray P.J., Anderson S.F., Sonu M.S., Parvin J.D., Young R.A.
      Nature 380:82-85(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
    2. "A human RNA polymerase II complex associated with SRB and DNA-repair proteins."
      Maldonado E., Shiekhattar R., Sheldon M., Cho H., Drapkin R., Rickert P., Lees E., Anderson C.W., Linn S., Reinberg D.
      Nature 381:86-89(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RNA POLYMERASE II.
      Tissue: Liver.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    7. "Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs."
      Oelgeschlaeger T., Tao Y., Kang Y.K., Roeder R.G.
      Mol. Cell 1:925-931(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription."
      Sun X., Zhang Y., Cho H., Rickert P., Lees E., Lane W.S., Reinberg D.
      Mol. Cell 2:213-222(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A FORM OF THE MEDIATOR COMPLEX.
    9. "The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex."
      Xiao H., Tao Y., Roeder R.G.
      J. Biol. Chem. 274:3937-3940(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNA POLYMERASE II AND MED21.
    10. "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."
      Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G.
      Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMCC COMPLEX.
    11. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    12. "hSrb7, an essential human Mediator component, acts as a coactivator for the thyroid hormone receptor."
      Nevado J., Tenbaum S.P., Aranda A.
      Mol. Cell. Endocrinol. 222:41-51(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH THRA.
    13. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1; MED6; MED12; MED13; MED16; MED17; MED18; MED20; MED24; MED28 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED21_HUMAN
    AccessioniPrimary (citable) accession number: Q13503
    Secondary accession number(s): B2R4I3, Q6IB05, Q92811
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3