Reviewed, UniProtKB/Swiss-Prot Q13489 (BIRC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Baculoviral IAP repeat-containing protein 3
Alternative name(s):
    Inhibitor of apoptosis protein 1
      Short name=HIAP-1
      Short name=HIAP1
    C-IAP2
    TNFR2-TRAF-signaling complex protein 1
    IAP homolog C
    Apoptosis inhibitor 2
      Short name=API2
    RING finger protein 49

Gene names

Name:	BIRC3
Synonyms:	API2, IAP1, MIHC, RNF49

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
Subunit structure	Interacts with SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity.
Subcellular location	CytoplasmPotential.
Tissue specificity	Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
Involvement in disease	A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
Sequence similarities	Belongs to the IAP family. Contains 3 BIR repeats. Contains 1 CARD domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Chromosomal rearrangement Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure
Gene Ontology (GO)
Biological process	anti-apoptosis Ref.2 Traceable author statement. Source: ProtInc cell surface receptor linked signal transduction Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB ubiquitin-protein ligase activity Inferred from direct assay. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DIABLO	Q9NR28	1	EBI-517709,EBI-517508
TRAF2	Q12933	2	EBI-517709,EBI-355744

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 604

604

Baculoviral IAP repeat-containing protein 3

PRO_0000122349

Regions

Repeat

29 – 96

68

BIR 1

Repeat

169 – 235

67

BIR 2

Repeat

255 – 322

68

BIR 3

Domain

439 – 529

91

CARD

Zinc finger

557 – 592

36

RING-type

Sites

Site

442 – 443

2

Breakpoint for translocation to form BIRC3-MALT1

Natural variations

Natural variant

260

1

K → R: dbSNP rs2276113.

VAR_021069

Natural variant

401

1

R → K: dbSNP rs17881197.

VAR_021070

Experimental info

Sequence conflict

18

1

N → Y in AAC83232. Ref.4

Sequence conflict

119

1

N → H in AAC50371. Ref.2

Sequence conflict

153

1

D → E in AAC50371. Ref.2

Sequence conflict

163

1

H → P Ref.2

Sequence conflict

165

1

A → P Ref.2

Sequence conflict

191

1

K → R in AAC50371. Ref.2

Sequence conflict

364

1

F → L in AAC50371. Ref.2

Sequence conflict

552

1

Q → P in AAC50371. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

604

Helix Strand Turn

Details...

Helix

250 – 252

3

Helix

255 – 260

6

Helix

261 – 264

4

Helix

273 – 278

6

Beta strand

281 – 285

5

Turn

286 – 288

3

Beta strand

289 – 292

4

Turn

293 – 295

3

Beta strand

298 – 301

4

Helix

308 – 315

8

Helix

320 – 334

15

Sequences

	Sequence		Length	Mass (Da)
	Q13489-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8581A00BA9AAB4A7	FASTA	604	68,372
	10 20 30 40 50 60 MNIVENSIFL SNLMKSANTF ELKYDLSCEL YRMSTYSTFP AGVPVSERSL ARAGFYYTGV 70 80 90 100 110 120 NDKVKCFCCG LMLDNWKRGD SPTEKHKKLY PSCRFVQSLN SVNNLEATSQ PTFPSSVTNS 130 140 150 160 170 180 THSLLPGTEN SGYFRGSYSN SPSNPVNSRA NQDFSALMRS SYHCAMNNEN ARLLTFQTWP 190 200 210 220 230 240 LTFLSPTDLA KAGFYYIGPG DRVACFACGG KLSNWEPKDN AMSEHLRHFP KCPFIENQLQ 250 260 270 280 290 300 DTSRYTVSNL SMQTHAARFK TFFNWPSSVL VNPEQLASAG FYYVGNSDDV KCFCCDGGLR 310 320 330 340 350 360 CWESGDDPWV QHAKWFPRCE YLIRIKGQEF IRQVQASYPH LLEQLLSTSD SPGDENAESS 370 380 390 400 410 420 IIHFEPGEDH SEDAIMMNTP VINAAVEMGF SRSLVKQTVQ RKILATGENY RLVNDLVLDL 430 440 450 460 470 480 LNAEDEIREE ERERATEEKE SNDLLLIRKN RMALFQHLTC VIPILDSLLT AGIINEQEHD 490 500 510 520 530 540 VIKQKTQTSL QARELIDTIL VKGNIAATVF RNSLQEAEAV LYEHLFVQQD IKYIPTEDVS 550 560 570 580 590 600 DLPVEEQLRR LQEERTCKVC MDKEVSIVFI PCGHLVVCKD CAPSLRKCPI CRSTIKGTVR TFLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins." Rothe M., Pan M.-G., Henzel W.J., Ayres T.M., Goeddel D.V. Cell 83:1243-1252(1995) [PubMed: 8548810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes." Liston P., Roy N., Tamai K., Lefebvre C., Baird S., Cherton-Horvat G., Farahani R., McLean M., Ikeda J., Mackenzie A., Korneluk R.G. Nature 379:349-353(1996) [PubMed: 8552191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors." Uren A.G., Pakusch M., Hawkins C.J., Puls K.L., Vaux D.L. Proc. Natl. Acad. Sci. U.S.A. 93:4974-4978(1996) [PubMed: 8643514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver.
[4]	"Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes." Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M., ten Cate J.W., Pannekoek H. Blood 93:3418-3431(1999) [PubMed: 10233894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Livingston R.J., Rieder M.J., Chung M.-W., Ritchie T.K., Olson A.N., Nguyen C.P., Nguyen D.A., Poel C.L., Chambers S.W., Schackwitz W.S., Sherwood J.K., Sherwood A.M., Leithauser B.J., Nickerson D.A. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-260 AND LYS-401.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[7]	"Structure of the MLT gene and molecular characterization of the genomic breakpoint junctions in the t(11;18)(q21;q21) of marginal zone B-cell lymphomas of MALT type." Baens M., Steyls A., Dierlamm J., De Wolf-Peeters C., Marynen P. Genes Chromosomes Cancer 29:281-291(2000) [PubMed: 11066071] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-441.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

L49432 mRNA. Translation: AAC41943.1.
U45878 mRNA. Translation: AAC50371.1.
U37546 mRNA. Translation: AAC50507.1.
AF070674 mRNA. Translation: AAC83232.1.
AY764389 Genomic DNA. Translation: AAU88144.1.
BC037420 mRNA. Translation: AAH37420.1.
AF178945 Genomic DNA. Translation: AAG09369.1.

PIR

S68449.

RefSeq

NP_001156.1.
NP_892007.1.

UniGene

Hs.127799

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2UVL	X-ray	1.91	A/B	244-337	[»]
3EB5	X-ray	2.00	A	536-604	[»]
3EB6	X-ray	3.40	A	536-604	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13489.

Protein family/group databases

MEROPS

I32.003.

PTM databases

PhosphoSite

Q13489.

Polymorphism databases

NIEHS-SNPs

Search...

Genome annotation databases

Ensembl

ENSG00000023445. Homo sapiens. [Contig view]

GeneID

330.

KEGG

hsa:330.

Organism-specific databases

H-InvDB

HIX0026135.

HGNC

HGNC:591. BIRC3.

HPA

HPA002317.

MIM

601721. gene.

PharmGKB

PA25360.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q13489.

HOVERGEN

Q13489.

Gene expression databases

ArrayExpress

Q13489.

CleanEx

HS_BIRC3.

GermOnline

ENSG00000023445. Homo sapiens.

Family and domain databases

InterPro

IPR001315. CARD.
IPR011029. DEATH_like.
IPR001370. Prot_inh_I32_IAP.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:1.10.533.10. DEATH_like. 1 hit.
G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

Pfam

PF00653. BIR. 3 hits.
PF00619. CARD. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]

SMART

SM00238. BIR. 3 hits.
SM00114. CARD. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]

PROSITE

PS01282. BIR_REPEAT_1. 3 hits.
PS50143. BIR_REPEAT_2. 3 hits.
PS50209. CARD. 1 hit.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

1359.

SOURCE

Search...

Entry information

Entry name

BIRC3_HUMAN

Accession

Primary (citable) accession number: Q13489
Secondary accession number(s): Q16628, Q9HC27, Q9UP46

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)