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Q13477 (MADCA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mucosal addressin cell adhesion molecule 1

Short name=MAdCAM-1
Short name=hMAdCAM-1
Gene names
Name:MADCAM1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Subunit structure

Homodimer Probable.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. Ref.1 Ref.2

Post-translational modification

The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation By similarity.

Polymorphism

The number of repeats in the mucin domain varies between 5 and 8 repeats.

Sequence similarities

Contains 2 Ig-like (immunoglobulin-like) domains.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q13477-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13477-2)

The sequence of this isoform differs from the canonical sequence as follows:
     223-334: VLHSPTSPEP...SSAVLGLLLL → A
Isoform 3 (identifier: Q13477-3)

The sequence of this isoform differs from the canonical sequence as follows:
     223-310: VLHSPTSPEP...TQGEVIPTGS → A
Note: No experimental confirmation available.
Isoform 4 (identifier: Q13477-4)

The sequence of this isoform differs from the canonical sequence as follows:
     18-112: Missing.
     223-310: VLHSPTSPEP...TQGEVIPTGS → A

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 382364Mucosal addressin cell adhesion molecule 1
PRO_0000014853

Regions

Topological domain19 – 317299Extracellular Potential
Transmembrane318 – 33821Helical; Potential
Topological domain339 – 38244Cytoplasmic Potential
Domain23 – 11290Ig-like 1
Domain113 – 231119Ig-like 2
Repeat228 – 23141; truncated
Repeat232 – 23982
Repeat240 – 24783
Repeat248 – 25584
Repeat256 – 26385
Repeat264 – 27186
Region226 – 31792Mucin-like
Region228 – 271445.5 X 8 AA tandem repeats of [PS]-P-D-T-T-S-[QP]-E

Amino acid modifications

Glycosylation831N-linked (GlcNAc...) Potential
Disulfide bond47 ↔ 94
Disulfide bond51 ↔ 98
Disulfide bond134 ↔ 204

Natural variations

Alternative sequence18 – 11295Missing in isoform 4.
VSP_047694
Alternative sequence223 – 334112VLHSP…GLLLL → A in isoform 2.
VSP_050014
Alternative sequence223 – 31088VLHSP…IPTGS → A in isoform 3 and isoform 4.
VSP_043202
Natural variant2531S → SPESPDTTSQEPPDTTSQEP PDTTS.
VAR_047901
Natural variant3001P → H.
Corresponds to variant rs3745925 [ dbSNP | Ensembl ].
VAR_017580

Experimental info

Sequence conflict2401S → P in AAB02194. Ref.2
Sequence conflict2401S → P in AAC51354. Ref.3
Sequence conflict2421D → N in AAB02194. Ref.2
Sequence conflict2421D → N in AAC51354. Ref.3
Sequence conflict2541Q → P in AAC13661. Ref.1

Secondary structure

...................................... 382
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 9DCA60C30BA61E62

FASTA38240,155
        10         20         30         40         50         60 
MDFGLALLLA GLLGLLLGQS LQVKPLQVEP PEPVVAVALG ASRQLTCRLA CADRGASVQW 

        70         80         90        100        110        120 
RGLDTSLGAV QSDTGRSVLT VRNASLSAAG TRVCVGSCGG RTFQHTVQLL VYAFPDQLTV 

       130        140        150        160        170        180 
SPAALVPGDP EVACTAHKVT PVDPNALSFS LLVGGQELEG AQALGPEVQE EEEEPQGDED 

       190        200        210        220        230        240 
VLFRVTERWR LPPLGTPVPP ALYCQATMRL PGLELSHRQA IPVLHSPTSP EPPDTTSPES 

       250        260        270        280        290        300 
PDTTSPESPD TTSQEPPDTT SPEPPDKTSP EPAPQQGSTH TPRSPGSTRT RRPEISQAGP 

       310        320        330        340        350        360 
TQGEVIPTGS SKPAGDQLPA ALWTSSAVLG LLLLALPTYH LWKRCRHLAE DDTHPPASLR 

       370        380 
LLPQVSAWAG LRGTGQVGIS PS 

« Hide

Isoform 2 [UniParc].

Checksum: 3613F1F208F9F31D
Show »

FASTA27128,705
Isoform 3 [UniParc].

Checksum: F635D6F54504D68D
Show »

FASTA29531,109
Isoform 4 [UniParc].

Checksum: 84204D98FC1FC7C7
Show »

FASTA20021,247

References

« Hide 'large scale' references
[1]"Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts."
Leung E., Greene J., Ni J., Raymond L.G., Lehnert K., Langley R., Krissansen G.W.
Immunol. Cell Biol. 74:490-496(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"Human mucosal addressin cell adhesion molecule-1 (MAdCAM-1) demonstrates structural and functional similarities to the alpha 4 beta 7-integrin binding domains of murine MAdCAM-1, but extreme divergence of mucin-like sequences."
Shyjan A.M., Bertagnolli M., Kenney C.J., Briskin M.J.
J. Immunol. 156:2851-2857(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN, TISSUE SPECIFICITY, VARIANT PRO-GLU-SER-PRO-ASP-THR-THR-SER-GLN-GLU-PRO-PRO-ASP-THR-THR-SER-GLN-GLU-PRO-PRO-ASP-THR-THR-SER-253 INS.
Tissue: Lymph node.
[3]"Genomic organization, chromosomal mapping, and analysis of the 5' promoter region of the human MAdCAM-1 gene."
Leung E., Berg R.W., Langley R., Greene J., Raymond L.A., Augustus M., Ni J., Carter K.C., Spurr N., Choo K.H.A., Krissansen G.W.
Immunogenetics 46:111-119(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), VARIANT PRO-GLU-SER-PRO-ASP-THR-THR-SER-GLN-GLU-PRO-PRO-ASP-THR-THR-SER-GLN-GLU-PRO-PRO-ASP-THR-THR-SER-253 INS.
Tissue: Placenta.
[4]"Novel splicing variants of some human genes."
Wang P.Z., Wang F., Chang Q.S., Wang X.
Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT PRO-GLU-SER-PRO-ASP-THR-THR-SER-GLN-GLU-PRO-PRO-ASP-THR-THR-SER-GLN-GLU-PRO-PRO-ASP-THR-THR-SER-253 INS.
Tissue: Brain.
[8]"The structure of immunoglobulin superfamily domains 1 and 2 of MAdCAM-1 reveals novel features important for integrin recognition."
Tan K., Casasnovas J.M., Liu J.H., Briskin M.J., Springer T.A., Wang J.H.
Structure 6:793-801(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 23-231.
[9]"A reassessment of the MAdCAM-1 structure and its role in integrin recognition."
Dando J., Wilkinson K.W., Ortlepp S., King D.J., Brady R.L.
Acta Crystallogr. D 58:233-241(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 23-224.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43628 mRNA. Translation: AAB02194.1.
U80016 expand/collapse EMBL AC list , U80012, U80013, U80014, U80015 Genomic DNA. Translation: AAC51354.1.
U82483 mRNA. Translation: AAC13661.1.
AY732484 mRNA. Translation: AAV33123.1.
AC005775 Genomic DNA. Translation: AAC62844.1.
CH471242 Genomic DNA. Translation: EAW61195.1.
BC137506 mRNA. Translation: AAI37507.1.
BC137507 mRNA. Translation: AAI37508.1.
BC144065 mRNA. Translation: AAI44066.1.
BC142629 mRNA. Translation: AAI42630.1.
CCDSCCDS12028.1. [Q13477-1]
CCDS12029.1. [Q13477-3]
RefSeqNP_570116.2. NM_130760.2. [Q13477-1]
NP_570118.1. NM_130762.2. [Q13477-3]
UniGeneHs.102598.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BQSX-ray2.20A23-231[»]
1GSMX-ray1.90A19-224[»]
4HBQX-ray1.40A/B23-171[»]
A/B180-224[»]
4HC1X-ray2.87A/B23-171[»]
A/B180-224[»]
4HCRX-ray2.30A/B23-225[»]
4HD9X-ray1.70A23-225[»]
ProteinModelPortalQ13477.
SMRQ13477. Positions 23-231.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113825. 1 interaction.
STRING9606.ENSP00000215637.

Chemistry

BindingDBQ13477.
ChEMBLCHEMBL4467.

Polymorphism databases

DMDM218511712.

Proteomic databases

PaxDbQ13477.
PRIDEQ13477.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000215637; ENSP00000215637; ENSG00000099866. [Q13477-1]
ENST00000346144; ENSP00000304247; ENSG00000099866. [Q13477-3]
ENST00000382683; ENSP00000372130; ENSG00000099866. [Q13477-4]
GeneID8174.
KEGGhsa:8174.
UCSCuc002los.3. human. [Q13477-1]
uc002lot.3. human. [Q13477-3]

Organism-specific databases

CTD8174.
GeneCardsGC19P000499.
H-InvDBHIX0014553.
HGNCHGNC:6765. MADCAM1.
MIM102670. gene.
neXtProtNX_Q13477.
PharmGKBPA30522.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG26033.
HOGENOMHOG000060103.
HOVERGENHBG108114.
InParanoidQ13477.
KOK06779.
OMAQAGPTQG.
OrthoDBEOG7FR7HJ.
TreeFamTF337571.

Enzyme and pathway databases

ReactomeREACT_118779. Extracellular matrix organization.
REACT_6900. Immune System.
SignaLinkQ13477.

Gene expression databases

ArrayExpressQ13477.
BgeeQ13477.
CleanExHS_MADCAM1.
GenevestigatorQ13477.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR015169. Adhes-Ig-like.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
PfamPF09085. Adhes-Ig_like. 1 hit.
[Graphical view]
ProDomPD336606. Adhes-Ig-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ13477.
GeneWikiAddressin.
GenomeRNAi8174.
NextBio30850.
PROQ13477.
SOURCESearch...

Entry information

Entry nameMADCA_HUMAN
AccessionPrimary (citable) accession number: Q13477
Secondary accession number(s): A5PKV4 expand/collapse secondary AC list , B2RPL9, O60222, O75867, Q5UGI7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: December 16, 2008
Last modified: July 9, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM