Reviewed,
UniProtKB/Swiss-Prot Q13472 (TOP3A_HUMAN)
Last modified
February 9, 2010.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA topoisomerase 3-alpha EC=5.99.1.2 Alternative name(s): DNA topoisomerase III alpha | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1001 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. |
| Catalytic activity | ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. |
| Subunit structure | Directly interacts with BLM and RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM. |
| Tissue specificity | High expression is found in testis, heart, skeletal muscle and pancreas. |
| Sequence similarities | Belongs to the prokaryotic type I/III topoisomerase family. Contains 1 Toprim domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Repeat Zinc-finger |
| Ligand | ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc |
| Molecular function | Isomerase Topoisomerase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replicationInferred from electronic annotation. Source: InterPro meiosis Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | PML body Inferred from direct assay. Source: UniProtKB chromosomeInferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activityInferred from electronic annotation. Source: EC protein bindingInferred from physical interaction. Source: UniProtKB zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: Q13472-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q13472-2) The sequence of this isoform differs from the canonical sequence as follows: 1-25: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1001 | 1001 | DNA topoisomerase 3-alpha | PRO_0000145190 | |||||
Regions | |||||||||
| Domain | 35 – 179 | 145 | Toprim | ||||||
| Repeat | 812 – 839 | 28 | 1 | ||||||
| Repeat | 896 – 923 | 28 | 2 | ||||||
| Zinc finger | 658 – 685 | 28 | C4-type Potential | ||||||
| Region | 812 – 923 | 112 | 2 X 27 AA approximate repeats | ||||||
Sites | |||||||||
| Active site | 362 | 1 | For DNA cleavage activity By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 54 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 771 | 1 | Phosphoserine Ref.8 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 25 | 25 | Missing in isoform Short. | VSP_006524 | |||||
| Natural variant | 459 | 1 | D → N: dbSNP rs28671051. | VAR_052588 | |||||
| Natural variant | 596 | 1 | C → Y | VAR_007529 | |||||
| Natural variant | 742 | 1 | D → N: dbSNP rs9909732. | VAR_052589 | |||||
| Natural variant | 773 | 1 | N → D: dbSNP rs9911283. | VAR_052590 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human TOP3: a single-copy gene encoding DNA topoisomerase III." Hanai R., Caron P.R., Wang J.C. Proc. Natl. Acad. Sci. U.S.A. 93:3653-3657(1996) [PubMed: 8622991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Trachea. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Testis. |
| [5] | "BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity." Yin J., Sobeck A., Xu C., Meetei A.R., Hoatlin M., Li L., Wang W. EMBO J. 24:1465-1476(2005) [PubMed: 15775963] [Abstract] Cited for: IDENTIFICATION IN COMPLEX WITH BLM AND RMI1. |
| [6] | "RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability." Xu D., Guo R., Sobeck A., Bachrati C.Z., Yang J., Enomoto T., Brown G.W., Hoatlin M.E., Hickson I.D., Wang W. Genes Dev. 22:2843-2855(2008) [PubMed: 18923082] [Abstract] Cited for: IDENTIFICATION IN THE RMI COMPLEX. |
| [7] | "BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome." Singh T.R., Ali A.M., Busygina V., Raynard S., Fan Q., Du C.-H., Andreassen P.R., Sung P., Meetei A.R. Genes Dev. 22:2856-2868(2008) [PubMed: 18923083] [Abstract] Cited for: IDENTIFICATION IN THE RMI COMPLEX. |
| [8] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-54 AND SER-771, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U43431 mRNA. Translation: AAB03694.1. U43431 mRNA. Translation: AAB03695.1. AK292926 mRNA. Translation: BAF85615.1. CH471196 Genomic DNA. Translation: EAW55646.1. BC051748 mRNA. Translation: AAH51748.1. |
| IPI | IPI00013378. IPI00221295. |
| RefSeq | NP_004609.1. |
| UniGene | Hs.592115 |
3D structure databases | |
| SMR | Q13472. Positions 35-612. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q13472. |
PTM databases | |
| PhosphoSite | Q13472. |
Proteomic databases | |
| PRIDE | Q13472. |
Genome annotation databases | |
| Ensembl | ENST00000321105; ENSP00000321636; ENSG00000177302; Homo sapiens. [Genome view] |
| GeneID | 7156. |
| KEGG | hsa:7156. |
| NMPDR | fig|9606.3.peg.13244. |
| UCSC | uc002gsx.1. human. |
Organism-specific databases | |
| CTD | 7156. |
| GeneCards | GC17M018117. |
| H-InvDB | HIX0027125. |
| HGNC | HGNC:11992. TOP3A. |
| HPA | CAB002449. |
| MIM | 601243. gene. |
| PharmGKB | PA36673. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG11894. |
| HOGENOM | HBG525547. |
| HOVERGEN | Q13472. |
| InParanoid | Q13472. |
| OMA | TVRKEGP. |
| OrthoDB | EOG9JT2RJ. |
| PhylomeDB | Q13472. |
Enzyme and pathway databases | |
| BRENDA | 5.99.1.2. 247. |
Gene expression databases | |
| ArrayExpress | Q13472. |
| Bgee | Q13472. |
| CleanEx | HS_TOP3A. |
| Genevestigator | Q13472. |
| GermOnline | ENSG00000177302. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. IPR001878. Znf_CCHC. [Graphical view] |
| Gene3D | G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit. |
| PANTHER | PTHR11390. Topo_IA. 1 hit. |
| Pfam | PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view] |
| PRINTS | PR00417. PRTPISMRASEI. |
| SMART | SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. SM00343. ZnF_C2HC. 1 hit. [Graphical view] |
| PROSITE | PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 28002. |
| SOURCE | Search... |
Entry information
| Entry name | TOP3A_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13472 Secondary accession number(s): A8KA61, Q13473 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 17 Human chromosome 17: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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