Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q13472

- TOP3A_HUMAN

UniProt

Q13472 - TOP3A_HUMAN

Protein

DNA topoisomerase 3-alpha

Gene

TOP3A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.2 Publications

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.2 PublicationsPROSITE-ProRule annotation

    Cofactori

    Magnesium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei362 – 3621O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri658 – 68528C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: ProtInc
    2. DNA topoisomerase type I activity Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: UniProtKB
    2. meiotic nuclear division Source: ProtInc

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_27271. Meiotic recombination.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3-alpha (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III alpha
    Gene namesi
    Name:TOP3A
    Synonyms:TOP3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:11992. TOP3A.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. nucleus Source: ProtInc
    3. PML body Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36673.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10011001DNA topoisomerase 3-alphaPRO_0000145190Add
    BLAST

    Proteomic databases

    MaxQBiQ13472.
    PaxDbiQ13472.
    PRIDEiQ13472.

    PTM databases

    PhosphoSiteiQ13472.

    Expressioni

    Tissue specificityi

    High expression is found in testis, heart, skeletal muscle and pancreas.

    Gene expression databases

    ArrayExpressiQ13472.
    BgeeiQ13472.
    CleanExiHS_TOP3A.
    GenevestigatoriQ13472.

    Organism-specific databases

    HPAiCAB002449.
    HPA042910.

    Interactioni

    Subunit structurei

    Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.5 Publications

    Protein-protein interaction databases

    BioGridi113009. 18 interactions.
    DIPiDIP-33323N.
    IntActiQ13472. 10 interactions.
    MINTiMINT-6610291.
    STRINGi9606.ENSP00000321636.

    Structurei

    Secondary structure

    1
    1001
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 308
    Beta strandi35 – 428
    Helixi43 – 5412
    Beta strandi59 – 613
    Beta strandi70 – 778
    Beta strandi80 – 889
    Beta strandi95 – 984
    Helixi100 – 1034
    Helixi105 – 1073
    Helixi110 – 1145
    Beta strandi118 – 1214
    Helixi124 – 1263
    Helixi127 – 13913
    Beta strandi141 – 1477
    Helixi151 – 16515
    Beta strandi172 – 1754
    Helixi183 – 1919
    Helixi198 – 22932
    Helixi231 – 2344
    Helixi245 – 26117
    Beta strandi266 – 27611
    Beta strandi279 – 2857
    Helixi293 – 30412
    Beta strandi309 – 32113
    Helixi329 – 33810
    Helixi344 – 35613
    Helixi376 – 3816
    Turni382 – 3854
    Turni387 – 3893
    Helixi390 – 3989
    Helixi428 – 44417
    Beta strandi449 – 46012
    Beta strandi463 – 47412
    Helixi476 – 4794
    Beta strandi503 – 5119
    Helixi520 – 53011
    Turni534 – 5374
    Helixi538 – 54710
    Beta strandi550 – 5534
    Beta strandi559 – 5613
    Helixi563 – 57311
    Helixi583 – 59614
    Helixi602 – 62221
    Helixi625 – 63511

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4CGYX-ray2.85A2-753[»]
    4CHTX-ray3.25A2-753[»]
    ProteinModelPortaliQ13472.
    SMRiQ13472. Positions 21-679.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 179145ToprimPROSITE-ProRule annotationAdd
    BLAST
    Repeati812 – 839281Add
    BLAST
    Repeati896 – 923282Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni812 – 9231122 X 27 AA approximate repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri658 – 68528C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0550.
    HOGENOMiHOG000184377.
    HOVERGENiHBG056668.
    InParanoidiQ13472.
    KOiK03165.
    OMAiLYQICME.
    OrthoDBiEOG74R1Q0.
    PhylomeDBiQ13472.
    TreeFamiTF105287.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 3 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR006171. Toprim_domain.
    IPR001878. Znf_CCHC.
    IPR010666. Znf_GRF.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    PF06839. zf-GRF. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q13472-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIFPVARYAL RWLRRPEDRA FSRAAMEMAL RGVRKVLCVA EKNDAAKGIA     50
    DLLSNGRMRR REGLSKFNKI YEFDYHLYGQ NVTMVMTSVS GHLLAHDFQM 100
    QFRKWQSCNP LVLFEAEIEK YCPENFVDIK KTLERETRQC QALVIWTDCD 150
    REGENIGFEI IHVCKAVKPN LQVLRARFSE ITPHAVRTAC ENLTEPDQRV 200
    SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS YGSCQFPTLG 250
    FVVERFKAIQ AFVPEIFHRI KVTHDHKDGI VEFNWKRHRL FNHTACLVLY 300
    QLCVEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI 350
    AEKLYTQGYI SYPRTETNIF PRDLNLTVLV EQQTPDPRWG AFAQSILERG 400
    GPTPRNGNKS DQAHPPIHPT KYTNNLQGDE QRLYEFIVRH FLACCSQDAQ 450
    GQETTVEIDI AQERFVAHGL MILARNYLDV YPYDHWSDKI LPVYEQGSHF 500
    QPSTVEMVDG ETSPPKLLTE ADLIALMEKH GIGTDATHAE HIETIKARMY 550
    VGLTPDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICDGKK 600
    DKFVVLRQQV QKYKQVFIEA VAKAKKLDEA LAQYFGNGTE LAQQEDIYPA 650
    MPEPIRKCPQ CNKDMVLKTK KNGGFYLSCM GFPECRSAVW LPDSVLEASR 700
    DSSVCPVCQP HPVYRLKLKF KRGSLPPTMP LEFVCCIGGC DDTLREILDL 750
    RFSGGPPRAS QPSGRLQANQ SLNRMDNSQH PQPADSRQTG SSKALAQTLP 800
    PPTAAGESNS VTCNCGQEAV LLTVRKEGPN RGRQFFKCNG GSCNFFLWAD 850
    SPNPGAGGPP ALAYRPLGAS LGCPPGPGIH LGGFGNPGDG SGSGTSCLCS 900
    QPSVTRTVQK DGPNKGRQFH TCAKPREQQC GFFQWVDENT APGTSGAPSW 950
    TGDRGRTLES EARSKRPRAS SSDMGSTAKK PRKCSLCHQP GHTRPFCPQN 1000
    R 1001
    Length:1,001
    Mass (Da):112,372
    Last modified:November 1, 1997 - v1
    Checksum:i06558C749569E0C2
    GO
    Isoform Short (identifier: Q13472-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-25: Missing.

    Show »
    Length:976
    Mass (Da):109,341
    Checksum:i4AB9262B5DE7D05B
    GO
    Isoform 3 (identifier: Q13472-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-105: MIFPVARYAL...HDFQMQFRKW → MNLIIICMAR

    Note: No experimental confirmation available.

    Show »
    Length:906
    Mass (Da):101,208
    Checksum:i60F389C5FF1A92F9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti459 – 4591D → N.
    Corresponds to variant rs28671051 [ dbSNP | Ensembl ].
    VAR_052588
    Natural varianti596 – 5961C → Y.
    VAR_007529
    Natural varianti742 – 7421D → N.
    Corresponds to variant rs9909732 [ dbSNP | Ensembl ].
    VAR_052589
    Natural varianti773 – 7731N → D.
    Corresponds to variant rs9911283 [ dbSNP | Ensembl ].
    VAR_052590

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 105105MIFPV…QFRKW → MNLIIICMAR in isoform 3. 1 PublicationVSP_054189Add
    BLAST
    Alternative sequencei1 – 2525Missing in isoform Short. 1 PublicationVSP_006524Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43431 mRNA. Translation: AAB03694.1.
    U43431 mRNA. Translation: AAB03695.1.
    HQ205026 Genomic DNA. Translation: ADP90454.1.
    HQ205027 Genomic DNA. Translation: ADP90455.1.
    HQ205028 Genomic DNA. Translation: ADP90456.1.
    HQ205029 Genomic DNA. Translation: ADP90457.1.
    HQ205030 Genomic DNA. Translation: ADP90458.1.
    HQ205031 Genomic DNA. Translation: ADP90459.1.
    HQ205032 Genomic DNA. Translation: ADP90460.1.
    HQ205033 Genomic DNA. Translation: ADP90461.1.
    HQ205034 Genomic DNA. Translation: ADP90462.1.
    HQ205035 Genomic DNA. Translation: ADP90463.1.
    HQ205036 Genomic DNA. Translation: ADP90464.1.
    HQ205037 Genomic DNA. Translation: ADP90465.1.
    HQ205038 Genomic DNA. Translation: ADP90466.1.
    HQ205039 Genomic DNA. Translation: ADP90467.1.
    HQ205040 Genomic DNA. Translation: ADP90468.1.
    HQ205041 Genomic DNA. Translation: ADP90469.1.
    HQ205042 Genomic DNA. Translation: ADP90470.1.
    HQ205043 Genomic DNA. Translation: ADP90471.1.
    HQ205044 Genomic DNA. Translation: ADP90472.1.
    HQ205045 Genomic DNA. Translation: ADP90473.1.
    HQ205046 Genomic DNA. Translation: ADP90474.1.
    HQ205047 Genomic DNA. Translation: ADP90475.1.
    HQ205048 Genomic DNA. Translation: ADP90476.1.
    HQ205049 Genomic DNA. Translation: ADP90477.1.
    HQ205050 Genomic DNA. Translation: ADP90478.1.
    HQ205051 Genomic DNA. Translation: ADP90479.1.
    HQ205052 Genomic DNA. Translation: ADP90480.1.
    HQ205053 Genomic DNA. Translation: ADP90481.1.
    HQ205054 Genomic DNA. Translation: ADP90482.1.
    HQ205055 Genomic DNA. Translation: ADP90483.1.
    HQ205056 Genomic DNA. Translation: ADP90484.1.
    HQ205057 Genomic DNA. Translation: ADP90485.1.
    HQ205058 Genomic DNA. Translation: ADP90486.1.
    HQ205059 Genomic DNA. Translation: ADP90487.1.
    HQ205060 Genomic DNA. Translation: ADP90488.1.
    HQ205061 Genomic DNA. Translation: ADP90489.1.
    HQ205062 Genomic DNA. Translation: ADP90490.1.
    HQ205063 Genomic DNA. Translation: ADP90491.1.
    HQ205064 Genomic DNA. Translation: ADP90492.1.
    HQ205065 Genomic DNA. Translation: ADP90493.1.
    AK292926 mRNA. Translation: BAF85615.1.
    AK296437 mRNA. Translation: BAG59092.1.
    AC127537 Genomic DNA. No translation available.
    CH471196 Genomic DNA. Translation: EAW55646.1.
    CH471196 Genomic DNA. Translation: EAW55648.1.
    CH471196 Genomic DNA. Translation: EAW55649.1.
    BC051748 mRNA. Translation: AAH51748.1.
    CCDSiCCDS11194.1. [Q13472-1]
    RefSeqiNP_004609.1. NM_004618.3. [Q13472-1]
    XP_005256833.1. XM_005256776.1. [Q13472-3]
    UniGeneiHs.592115.

    Genome annotation databases

    EnsembliENST00000321105; ENSP00000321636; ENSG00000177302. [Q13472-1]
    ENST00000542570; ENSP00000442336; ENSG00000177302. [Q13472-3]
    ENST00000580095; ENSP00000462790; ENSG00000177302. [Q13472-2]
    GeneIDi7156.
    KEGGihsa:7156.
    UCSCiuc002gsx.1. human. [Q13472-1]

    Polymorphism databases

    DMDMi2501242.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43431 mRNA. Translation: AAB03694.1 .
    U43431 mRNA. Translation: AAB03695.1 .
    HQ205026 Genomic DNA. Translation: ADP90454.1 .
    HQ205027 Genomic DNA. Translation: ADP90455.1 .
    HQ205028 Genomic DNA. Translation: ADP90456.1 .
    HQ205029 Genomic DNA. Translation: ADP90457.1 .
    HQ205030 Genomic DNA. Translation: ADP90458.1 .
    HQ205031 Genomic DNA. Translation: ADP90459.1 .
    HQ205032 Genomic DNA. Translation: ADP90460.1 .
    HQ205033 Genomic DNA. Translation: ADP90461.1 .
    HQ205034 Genomic DNA. Translation: ADP90462.1 .
    HQ205035 Genomic DNA. Translation: ADP90463.1 .
    HQ205036 Genomic DNA. Translation: ADP90464.1 .
    HQ205037 Genomic DNA. Translation: ADP90465.1 .
    HQ205038 Genomic DNA. Translation: ADP90466.1 .
    HQ205039 Genomic DNA. Translation: ADP90467.1 .
    HQ205040 Genomic DNA. Translation: ADP90468.1 .
    HQ205041 Genomic DNA. Translation: ADP90469.1 .
    HQ205042 Genomic DNA. Translation: ADP90470.1 .
    HQ205043 Genomic DNA. Translation: ADP90471.1 .
    HQ205044 Genomic DNA. Translation: ADP90472.1 .
    HQ205045 Genomic DNA. Translation: ADP90473.1 .
    HQ205046 Genomic DNA. Translation: ADP90474.1 .
    HQ205047 Genomic DNA. Translation: ADP90475.1 .
    HQ205048 Genomic DNA. Translation: ADP90476.1 .
    HQ205049 Genomic DNA. Translation: ADP90477.1 .
    HQ205050 Genomic DNA. Translation: ADP90478.1 .
    HQ205051 Genomic DNA. Translation: ADP90479.1 .
    HQ205052 Genomic DNA. Translation: ADP90480.1 .
    HQ205053 Genomic DNA. Translation: ADP90481.1 .
    HQ205054 Genomic DNA. Translation: ADP90482.1 .
    HQ205055 Genomic DNA. Translation: ADP90483.1 .
    HQ205056 Genomic DNA. Translation: ADP90484.1 .
    HQ205057 Genomic DNA. Translation: ADP90485.1 .
    HQ205058 Genomic DNA. Translation: ADP90486.1 .
    HQ205059 Genomic DNA. Translation: ADP90487.1 .
    HQ205060 Genomic DNA. Translation: ADP90488.1 .
    HQ205061 Genomic DNA. Translation: ADP90489.1 .
    HQ205062 Genomic DNA. Translation: ADP90490.1 .
    HQ205063 Genomic DNA. Translation: ADP90491.1 .
    HQ205064 Genomic DNA. Translation: ADP90492.1 .
    HQ205065 Genomic DNA. Translation: ADP90493.1 .
    AK292926 mRNA. Translation: BAF85615.1 .
    AK296437 mRNA. Translation: BAG59092.1 .
    AC127537 Genomic DNA. No translation available.
    CH471196 Genomic DNA. Translation: EAW55646.1 .
    CH471196 Genomic DNA. Translation: EAW55648.1 .
    CH471196 Genomic DNA. Translation: EAW55649.1 .
    BC051748 mRNA. Translation: AAH51748.1 .
    CCDSi CCDS11194.1. [Q13472-1 ]
    RefSeqi NP_004609.1. NM_004618.3. [Q13472-1 ]
    XP_005256833.1. XM_005256776.1. [Q13472-3 ]
    UniGenei Hs.592115.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4CGY X-ray 2.85 A 2-753 [» ]
    4CHT X-ray 3.25 A 2-753 [» ]
    ProteinModelPortali Q13472.
    SMRi Q13472. Positions 21-679.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113009. 18 interactions.
    DIPi DIP-33323N.
    IntActi Q13472. 10 interactions.
    MINTi MINT-6610291.
    STRINGi 9606.ENSP00000321636.

    PTM databases

    PhosphoSitei Q13472.

    Polymorphism databases

    DMDMi 2501242.

    Proteomic databases

    MaxQBi Q13472.
    PaxDbi Q13472.
    PRIDEi Q13472.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000321105 ; ENSP00000321636 ; ENSG00000177302 . [Q13472-1 ]
    ENST00000542570 ; ENSP00000442336 ; ENSG00000177302 . [Q13472-3 ]
    ENST00000580095 ; ENSP00000462790 ; ENSG00000177302 . [Q13472-2 ]
    GeneIDi 7156.
    KEGGi hsa:7156.
    UCSCi uc002gsx.1. human. [Q13472-1 ]

    Organism-specific databases

    CTDi 7156.
    GeneCardsi GC17M018175.
    HGNCi HGNC:11992. TOP3A.
    HPAi CAB002449.
    HPA042910.
    MIMi 601243. gene.
    neXtProti NX_Q13472.
    PharmGKBi PA36673.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0550.
    HOGENOMi HOG000184377.
    HOVERGENi HBG056668.
    InParanoidi Q13472.
    KOi K03165.
    OMAi LYQICME.
    OrthoDBi EOG74R1Q0.
    PhylomeDBi Q13472.
    TreeFami TF105287.

    Enzyme and pathway databases

    Reactomei REACT_27271. Meiotic recombination.

    Miscellaneous databases

    GeneWikii TOP3A.
    GenomeRNAii 7156.
    NextBioi 28002.
    PROi Q13472.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q13472.
    Bgeei Q13472.
    CleanExi HS_TOP3A.
    Genevestigatori Q13472.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 3 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR006171. Toprim_domain.
    IPR001878. Znf_CCHC.
    IPR010666. Znf_GRF.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    PF06839. zf-GRF. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human TOP3: a single-copy gene encoding DNA topoisomerase III."
      Hanai R., Caron P.R., Wang J.C.
      Proc. Natl. Acad. Sci. U.S.A. 93:3653-3657(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), FUNCTION, CATALYTIC ACTIVITY.
    2. "Identification of rare DNA variants in mitochondrial disorders with improved array-based sequencing."
      Wang W., Shen P., Thiyagarajan S., Lin S., Palm C., Horvath R., Klopstock T., Cutler D., Pique L., Schrijver I., Davis R.W., Mindrinos M., Speed T.P., Scharfe C.
      Nucleic Acids Res. 39:44-58(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND 3).
      Tissue: Thalamus and Trachea.
    4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Testis.
    7. "BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity."
      Yin J., Sobeck A., Xu C., Meetei A.R., Hoatlin M., Li L., Wang W.
      EMBO J. 24:1465-1476(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN COMPLEX WITH BLM AND RMI1.
    8. "RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability."
      Xu D., Guo R., Sobeck A., Bachrati C.Z., Yang J., Enomoto T., Brown G.W., Hoatlin M.E., Hickson I.D., Wang W.
      Genes Dev. 22:2843-2855(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RMI COMPLEX.
    9. "BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome."
      Singh T.R., Ali A.M., Busygina V., Raynard S., Fan Q., Du C.-H., Andreassen P.R., Sung P., Meetei A.R.
      Genes Dev. 22:2856-2868(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RMI COMPLEX.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1."
      Yang J., Bachrati C.Z., Ou J., Hickson I.D., Brown G.W.
      J. Biol. Chem. 285:21426-21436(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, COFACTOR, INTERACTION WITH RMI1.
    12. "Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair."
      Wan L., Han J., Liu T., Dong S., Xie F., Chen H., Huang J.
      Proc. Natl. Acad. Sci. U.S.A. 110:10646-10651(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BLM.

    Entry informationi

    Entry nameiTOP3A_HUMAN
    AccessioniPrimary (citable) accession number: Q13472
    Secondary accession number(s): A8KA61
    , B4DK80, D3DXC7, Q13473
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3