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Protein

Golgin subfamily A member 4

Gene

GOLGA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1.1 Publication

GO - Molecular functioni

  • GTPase binding Source: UniProtKB

GO - Biological processi

  • Golgi to plasma membrane protein transport Source: UniProtKB
  • positive regulation of axon extension Source: UniProtKB
  • protein targeting to Golgi Source: InterPro
  • vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000144674-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgin subfamily A member 4
Alternative name(s):
256 kDa golgin
Golgin-245
Protein 72.1
Trans-Golgi p230
Gene namesi
Name:GOLGA4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:4427. GOLGA4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA
  • trans-Golgi network Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2177Y → A: Loss of Golgi membrane localization. Loss of ARL1-binding. 2 Publications1
Mutagenesisi2177Y → F: No effect. 2 Publications1
Mutagenesisi2181V → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2183F → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2186M → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2193T → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2194M → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2197V → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2198I → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2202L → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2204F → A: Abolishes Golgi localization. 1 Publication1
Mutagenesisi2212I → A: Abolishes Golgi localization. 1 Publication1

Organism-specific databases

DisGeNETi2803.
OpenTargetsiENSG00000144674.
PharmGKBiPA28808.

Polymorphism and mutation databases

BioMutaiGOLGA4.
DMDMi12643718.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001900591 – 2230Golgin subfamily A member 4Add BLAST2230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei39PhosphothreonineBy similarity1
Modified residuei41PhosphoserineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei89PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Glycosylationi585N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi1612N-linked (GlcNAc...)PROSITE-ProRule annotation1
Modified residuei2223PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ13439.
MaxQBiQ13439.
PaxDbiQ13439.
PeptideAtlasiQ13439.
PRIDEiQ13439.

PTM databases

iPTMnetiQ13439.
PhosphoSitePlusiQ13439.
UniCarbKBiQ13439.

Expressioni

Gene expression databases

BgeeiENSG00000144674.
CleanExiHS_GOLGA4.
ExpressionAtlasiQ13439. baseline and differential.
GenevisibleiQ13439. HS.

Organism-specific databases

HPAiHPA035102.
HPA040675.

Interactioni

Subunit structurei

Homodimer. Interacts with GTP bound ARL1. Interacts with MACF1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKRD27Q96NW42EBI-1037845,EBI-6125599

GO - Molecular functioni

  • GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109065. 31 interactors.
IntActiQ13439. 20 interactors.
MINTiMINT-3027757.
STRINGi9606.ENSP00000349305.

Structurei

Secondary structure

12230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2173 – 2186Combined sources14
Helixi2191 – 2201Combined sources11
Helixi2206 – 2225Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4AX-ray2.30E/F/G/H2172-2222[»]
1UPTX-ray1.70B/D/F/H2170-2228[»]
ProteinModelPortaliQ13439.
SMRiQ13439.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13439.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2168 – 2215GRIPPROSITE-ProRule annotationAdd BLAST48

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 203Interaction with MACF11 PublicationAdd BLAST71

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili133 – 2185Sequence analysisAdd BLAST2053

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 2096Glu-richAdd BLAST1845

Domaini

Extended rod-like protein with coiled-coil domains.

Sequence similaritiesi

Contains 1 GRIP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEA5. Eukaryota.
ENOG410XQQG. LUCA.
GeneTreeiENSGT00730000111139.
HOGENOMiHOG000112753.
HOVERGENiHBG051754.
InParanoidiQ13439.
KOiK20283.
OMAiNEEYVES.
OrthoDBiEOG091G009M.
PhylomeDBiQ13439.
TreeFamiTF325082.

Family and domain databases

Gene3Di1.10.220.60. 1 hit.
InterProiIPR000237. GRIP_dom.
[Graphical view]
PfamiPF01465. GRIP. 1 hit.
[Graphical view]
SMARTiSM00755. Grip. 1 hit.
[Graphical view]
SUPFAMiSSF101283. SSF101283. 1 hit.
PROSITEiPS50913. GRIP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q13439-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFKKLKQKIS EEQQQLQQAL APAQASSNSS TPTRMRSRTS SFTEQLDEGT
60 70 80 90 100
PNRESGDTQS FAQKLQLRVP SVESLFRSPI KESLFRSSSK ESLVRTSSRE
110 120 130 140 150
SLNRLDLDSS TASFDPPSDM DSEAEDLVGN SDSLNKEQLI QRLRRMERSL
160 170 180 190 200
SSYRGKYSEL VTAYQMLQRE KKKLQGILSQ SQDKSLRRIA ELREELQMDQ
210 220 230 240 250
QAKKHLQEEF DASLEEKDQY ISVLQTQVSL LKQRLRNGPM NVDVLKPLPQ
260 270 280 290 300
LEPQAEVFTK EENPESDGEP VVEDGTSVKT LETLQQRVKR QENLLKRCKE
310 320 330 340 350
TIQSHKEQCT LLTSEKEALQ EQLDERLQEL EKIKDLHMAE KTKLITQLRD
360 370 380 390 400
AKNLIEQLEQ DKGMVIAETK RQMHETLEMK EEEIAQLRSR IKQMTTQGEE
410 420 430 440 450
LREQKEKSER AAFEELEKAL STAQKTEEAR RKLKAEMDEQ IKTIEKTSEE
460 470 480 490 500
ERISLQQELS RVKQEVVDVM KKSSEEQIAK LQKLHEKELA RKEQELTKKL
510 520 530 540 550
QTREREFQEQ MKVALEKSQS EYLKISQEKE QQESLALEEL ELQKKAILTE
560 570 580 590 600
SENKLRDLQQ EAETYRTRIL ELESSLEKSL QENKNQSKDL AVHLEAEKNK
610 620 630 640 650
HNKEITVMVE KHKTELESLK HQQDALWTEK LQVLKQQYQT EMEKLREKCE
660 670 680 690 700
QEKETLLKDK EIIFQAHIEE MNEKTLEKLD VKQTELESLS SELSEVLKAR
710 720 730 740 750
HKLEEELSVL KDQTDKMKQE LEAKMDEQKN HHQQQVDSII KEHEVSIQRT
760 770 780 790 800
EKALKDQINQ LELLLKERDK HLKEHQAHVE NLEADIKRSE GELQQASAKL
810 820 830 840 850
DVFQSYQSAT HEQTKAYEEQ LAQLQQKLLD LETERILLTK QVAEVEAQKK
860 870 880 890 900
DVCTELDAHK IQVQDLMQQL EKQNSEMEQK VKSLTQVYES KLEDGNKEQE
910 920 930 940 950
QTKQILVEKE NMILQMREGQ KKEIEILTQK LSAKEDSIHI LNEEYETKFK
960 970 980 990 1000
NQEKKMEKVK QKAKEMQETL KKKLLDQEAK LKKELENTAL ELSQKEKQFN
1010 1020 1030 1040 1050
AKMLEMAQAN SAGISDAVSR LETNQKEQIE SLTEVHRREL NDVISIWEKK
1060 1070 1080 1090 1100
LNQQAEELQE IHEIQLQEKE QEVAELKQKI LLFGCEKEEM NKEITWLKEE
1110 1120 1130 1140 1150
GVKQDTTLNE LQEQLKQKSA HVNSLAQDET KLKAHLEKLE VDLNKSLKEN
1160 1170 1180 1190 1200
TFLQEQLVEL KMLAEEDKRK VSELTSKLKT TDEEFQSLKS SHEKSNKSLE
1210 1220 1230 1240 1250
DKSLEFKKLS EELAIQLDIC CKKTEALLEA KTNELINISS SKTNAILSRI
1260 1270 1280 1290 1300
SHCQHRTTKV KEALLIKTCT VSELEAQLRQ LTEEQNTLNI SFQQATHQLE
1310 1320 1330 1340 1350
EKENQIKSMK ADIESLVTEK EALQKEGGNQ QQAASEKESC ITQLKKELSE
1360 1370 1380 1390 1400
NINAVTLMKE ELKEKKVEIS SLSKQLTDLN VQLQNSISLS EKEAAISSLR
1410 1420 1430 1440 1450
KQYDEEKCEL LDQVQDLSFK VDTLSKEKIS ALEQVDDWSN KFSEWKKKAQ
1460 1470 1480 1490 1500
SRFTQHQNTV KELQIQLELK SKEAYEKDEQ INLLKEELDQ QNKRFDCLKG
1510 1520 1530 1540 1550
EMEDDKSKME KKESNLETEL KSQTARIMEL EDHITQKTIE IESLNEVLKN
1560 1570 1580 1590 1600
YNQQKDIEHK ELVQKLQHFQ ELGEEKDNRV KEAEEKILTL ENQVYSMKAE
1610 1620 1630 1640 1650
LETKKKELEH VNLSVKSKEE ELKALEDRLE SESAAKLAEL KRKAEQKIAA
1660 1670 1680 1690 1700
IKKQLLSQME EKEEQYKKGT ESHLSELNTK LQEREREVHI LEEKLKSVES
1710 1720 1730 1740 1750
SQSETLIVPR SAKNVAAYTE QEEADSQGCV QKTYEEKISV LQRNLTEKEK
1760 1770 1780 1790 1800
LLQRVGQEKE ETVSSHFEMR CQYQERLIKL EHAEAKQHED QSMIGHLQEE
1810 1820 1830 1840 1850
LEEKNKKYSL IVAQHVEKEG GKNNIQAKQN LENVFDDVQK TLQEKELTCQ
1860 1870 1880 1890 1900
ILEQKIKELD SCLVRQKEVH RVEMEELTSK YEKLQALQQM DGRNKPTELL
1910 1920 1930 1940 1950
EENTEEKSKS HLVQPKLLSN MEAQHNDLEF KLAGAEREKQ KLGKEIVRLQ
1960 1970 1980 1990 2000
KDLRMLRKEH QQELEILKKE YDQEREEKIK QEQEDLELKH NSTLKQLMRE
2010 2020 2030 2040 2050
FNTQLAQKEQ ELEMTIKETI NKAQEVEAEL LESHQEETNQ LLKKIAEKDD
2060 2070 2080 2090 2100
DLKRTAKRYE EILDAREEEM TAKVRDLQTQ LEELQKKYQQ KLEQEENPGN
2110 2120 2130 2140 2150
DNVTIMELQT QLAQKTTLIS DSKLKEQEFR EQIHNLEDRL KKYEKNVYAT
2160 2170 2180 2190 2200
TVGTPYKGGN LYHTDVSLFG EPTEFEYLRK VLFEYMMGRE TKTMAKVITT
2210 2220 2230
VLKFPDDQTQ KILEREDARL MFTSPRSGIF
Length:2,230
Mass (Da):261,140
Last modified:November 1, 1996 - v1
Checksum:i3BB733DB1EA86134
GO
Isoform 3 (identifier: Q13439-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2103-2109: Missing.

Show »
Length:2,223
Mass (Da):260,325
Checksum:iB401B4C93E612E70
GO
Isoform 4 (identifier: Q13439-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2222-2230: FTSPRSGIF → SWLRSSS

Show »
Length:2,228
Mass (Da):260,951
Checksum:i9E1B1F1B13A6A218
GO
Isoform 5 (identifier: Q13439-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-54: E → ENASTHASKSPDSVNGSEPSIPQ
     2103-2109: Missing.
     2222-2230: FTSPRSGIF → SWLRSSS

Note: No experimental confirmation available.
Show »
Length:2,243
Mass (Da):262,328
Checksum:iEDD8BCB88E02B9A4
GO

Sequence cautioni

The sequence BAD92930 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA58041 differs from that shown. Reason: Frameshift at position 2153.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188R → K in AAC51791 (PubMed:8537393).Curated1
Sequence conflicti220Y → H in AAC51791 (PubMed:8537393).Curated1
Sequence conflicti276T → A in AAC51791 (PubMed:8537393).Curated1
Sequence conflicti584K → E in AAC51791 (PubMed:8537393).Curated1
Sequence conflicti628T → A in AAC51791 (PubMed:8537393).Curated1
Sequence conflicti630K → E in AAC51791 (PubMed:8537393).Curated1
Sequence conflicti682K → N in AAC51791 (PubMed:8537393).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0339751028Q → K.Corresponds to variant rs11718848dbSNPEnsembl.1
Natural variantiVAR_0339761552N → S.Corresponds to variant rs9840779dbSNPEnsembl.1
Natural variantiVAR_0492582058R → S.Corresponds to variant rs11924014dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04481954E → ENASTHASKSPDSVNGSEPS IPQ in isoform 5. 1 Publication1
Alternative sequenceiVSP_0042742103 – 2109Missing in isoform 3 and isoform 5. 1 Publication7
Alternative sequenceiVSP_0042752222 – 2230FTSPRSGIF → SWLRSSS in isoform 4 and isoform 5. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41740 mRNA. Translation: AAC50434.1.
X82834 mRNA. Translation: CAA58041.1. Frameshift.
AC097359 Genomic DNA. No translation available.
AB209693 mRNA. Translation: BAD92930.1. Different initiation.
U31906 mRNA. Translation: AAC51791.1.
X76942 mRNA. Translation: CAA54261.1.
CCDSiCCDS2666.1. [Q13439-1]
CCDS54564.1. [Q13439-5]
RefSeqiNP_001166184.1. NM_001172713.1. [Q13439-5]
NP_002069.2. NM_002078.4. [Q13439-1]
UniGeneiHs.344151.

Genome annotation databases

EnsembliENST00000356847; ENSP00000349305; ENSG00000144674. [Q13439-5]
ENST00000361924; ENSP00000354486; ENSG00000144674. [Q13439-1]
GeneIDi2803.
KEGGihsa:2803.
UCSCiuc003cgv.4. human. [Q13439-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41740 mRNA. Translation: AAC50434.1.
X82834 mRNA. Translation: CAA58041.1. Frameshift.
AC097359 Genomic DNA. No translation available.
AB209693 mRNA. Translation: BAD92930.1. Different initiation.
U31906 mRNA. Translation: AAC51791.1.
X76942 mRNA. Translation: CAA54261.1.
CCDSiCCDS2666.1. [Q13439-1]
CCDS54564.1. [Q13439-5]
RefSeqiNP_001166184.1. NM_001172713.1. [Q13439-5]
NP_002069.2. NM_002078.4. [Q13439-1]
UniGeneiHs.344151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4AX-ray2.30E/F/G/H2172-2222[»]
1UPTX-ray1.70B/D/F/H2170-2228[»]
ProteinModelPortaliQ13439.
SMRiQ13439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109065. 31 interactors.
IntActiQ13439. 20 interactors.
MINTiMINT-3027757.
STRINGi9606.ENSP00000349305.

PTM databases

iPTMnetiQ13439.
PhosphoSitePlusiQ13439.
UniCarbKBiQ13439.

Polymorphism and mutation databases

BioMutaiGOLGA4.
DMDMi12643718.

Proteomic databases

EPDiQ13439.
MaxQBiQ13439.
PaxDbiQ13439.
PeptideAtlasiQ13439.
PRIDEiQ13439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356847; ENSP00000349305; ENSG00000144674. [Q13439-5]
ENST00000361924; ENSP00000354486; ENSG00000144674. [Q13439-1]
GeneIDi2803.
KEGGihsa:2803.
UCSCiuc003cgv.4. human. [Q13439-1]

Organism-specific databases

CTDi2803.
DisGeNETi2803.
GeneCardsiGOLGA4.
HGNCiHGNC:4427. GOLGA4.
HPAiHPA035102.
HPA040675.
MIMi602509. gene.
neXtProtiNX_Q13439.
OpenTargetsiENSG00000144674.
PharmGKBiPA28808.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEA5. Eukaryota.
ENOG410XQQG. LUCA.
GeneTreeiENSGT00730000111139.
HOGENOMiHOG000112753.
HOVERGENiHBG051754.
InParanoidiQ13439.
KOiK20283.
OMAiNEEYVES.
OrthoDBiEOG091G009M.
PhylomeDBiQ13439.
TreeFamiTF325082.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000144674-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

ChiTaRSiGOLGA4. human.
EvolutionaryTraceiQ13439.
GeneWikiiGOLGA4.
GenomeRNAii2803.
PROiQ13439.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144674.
CleanExiHS_GOLGA4.
ExpressionAtlasiQ13439. baseline and differential.
GenevisibleiQ13439. HS.

Family and domain databases

Gene3Di1.10.220.60. 1 hit.
InterProiIPR000237. GRIP_dom.
[Graphical view]
PfamiPF01465. GRIP. 1 hit.
[Graphical view]
SMARTiSM00755. Grip. 1 hit.
[Graphical view]
SUPFAMiSSF101283. SSF101283. 1 hit.
PROSITEiPS50913. GRIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGOGA4_HUMAN
AccessioniPrimary (citable) accession number: Q13439
Secondary accession number(s): F8W8Q7
, Q13270, Q13654, Q14436, Q59EW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Antibodies against GOLGA4 are present in sera from patients with Sjoegren syndrome. Sera from patients with Sjoegren syndrome often contain antibodies that react with normal components of the Golgi complex.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.