Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q13423 (NNTM_HUMAN)

Last modified November 25, 2008. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    NAD(P) transhydrogenase, mitochondrial
    EC=1.6.1.2
Alternative name(s):
    Nicotinamide nucleotide transhydrogenase
    Pyridine nucleotide transhydrogenase
Gene names
Name: NNT
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length1086 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Catalytic activity

NADPH + NAD(+) = NADP(+) + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein; Matrix sidePotential.

Sequence similarities

In the N-terminal section; belongs to the AlaDH/PNT family.

In the C-terminal section; belongs to the PNT beta subunit family.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion By similarity
Chain44 – 10861043NAD(P) transhydrogenase, mitochondrial
PRO_0000001055

Regions

Topological domain44 – 474431Mitochondrial matrix
Transmembrane475 – 49319 Potential
Transmembrane501 – 52121 Potential
Transmembrane527 – 54620 Potential
Transmembrane558 – 57821 Potential
Topological domain579 – 59517Mitochondrial matrix
Transmembrane596 – 61621 Potential
Transmembrane622 – 64221 Potential
Transmembrane646 – 66621 Potential
Transmembrane672 – 69120 Potential
Transmembrane702 – 72221 Potential
Topological domain723 – 73917Cytoplasmic
Transmembrane740 – 76021 Potential
Transmembrane778 – 79720 Potential
Transmembrane801 – 81919 Potential
Transmembrane833 – 85321 Potential
Transmembrane857 – 87923 Potential
Topological domain880 – 1086207Mitochondrial matrix
Nucleotide binding229 – 25931NAD By similarity

Experimental info

Sequence conflict1761A → T in CAA90428. Ref.2
Sequence conflict2121G → E in CAD38536. Ref.3
Sequence conflict2461A → E in AAC51914. Ref.1
Sequence conflict2621A → S in AAC51914. Ref.1
Sequence conflict7061F → S in AAC51914. Ref.1
Sequence conflict7311T → P in AAC51914. Ref.1
Sequence conflict8101S → A in CAA90428. Ref.2
Sequence conflict8241A → P in CAA90428. Ref.2
Sequence conflict8711I → P in AAC51914. Ref.1
Sequence conflict9291I → F in AAC51914. Ref.1
Sequence conflict10591K → R in CAD38536. Ref.3

Secondary structure

1 ....................................... 1086
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q13423-1 [UniParc].

Last modified August 16, 2004. Version 3.
Checksum: 8A437658CA0EB41B

FASTA1,086113,896
        10         20         30         40         50         60 
MANLLKTVVT GCSCPLLSNL GSCKGLRVKK DFLRTFYTHQ ELWCKAPVKP GIPYKQLTVG 

        70         80         90        100        110        120 
VPKEIFQNEK RVALSPAGVQ NLVKQGFNVV VESGAGEASK FSDDHYRVAG AQIQGAKEVL 

       130        140        150        160        170        180 
ASDLVVKVRA PMVNPTLGVH EADLLKTSGT LISFIYPAQN PELLNKLSQR KTTVLAMDQV 

       190        200        210        220        230        240 
PRVTIAQGYD ALSSMANIAG YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL 

       250        260        270        280        290        300 
ASAGAAKSMG AIVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE 

       310        320        330        340        350        360 
MKLFAQQCKE VDILISTALI PGKKAPVLFN KEMIESMKEG SVVVDLAAEA GGNFETTKPG 

       370        380        390        400        410        420 
ELYIHKGITH IGYTDLPSRM ATQASTLYSN NITKLLKAIS PDKDNFYFDV KDDFDFGTMG 

       430        440        450        460        470        480 
HVIRGTVVMK DGKVIFPAPT PKNIPQGAPV KQKTVAELEA EKAATITPFR KTMSTASAYT 

       490        500        510        520        530        540 
AGLTGILGLG IAAPNLAFSQ MVTTFGLAGI VGYHTVWGVT PALHSPLMSV TNAISGLTAV 

       550        560        570        580        590        600 
GGLALMGGHL YPSTTSQGLA ALAAFISSVN IAGGFLVTQR MLDMFKRPTD PPEYNYLYLL 

       610        620        630        640        650        660 
PAGTFVGGYL AALYSGYNIE QIMYLGSGLC CVGALAGLST QGTARLGNAL GMIGVAGGLA 

       670        680        690        700        710        720 
ATLGVLKPGP ELLAQMSGAM ALGGTIGLTI AKRIQISDLP QLVAAFHSLV GLAAVLTCIA 

       730        740        750        760        770        780 
EYIIEYPHFA TDAAANLTKI VAYLGTYIGG VTFSGSLIAY GKLQGLLKSA PLLLPGRHLL 

       790        800        810        820        830        840 
NAGLLAASVG GIIPFMVDPS FTTGITCLGS VSALSAVMGV TLTAAIGGAD MPVVITVLNS 

       850        860        870        880        890        900 
YSGWALCAEG FLLNNNLLTI VGALIGSSGA ILSYIMCVAM NRSLANVILG GYGTTSTAGG 

       910        920        930        940        950        960 
KPMEISGTHT EINLDNAIDM IREANSIIIT PGYGLCAAKA QYPIADLVKM LTEQGKKVRF 

       970        980        990       1000       1010       1020 
GIHPVAGRMP GQLNVLLAEA GVPYDIVLEM DEINHDFPDT DLVLVIGAND TVNSAAQEDP 

      1030       1040       1050       1060       1070       1080 
NSIIAGMPVL EVWKSKQVIV MKRSLGVGYA AVDNPIFYKP NTAMLLGDAK KTCDALQAKV 


RESYQK

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Cloning and deduced amino acid sequence of human nicotinamide nucleotide transhydrogenase."
Ware J., Zieger B.
DNA Seq. 7:369-374(1997) [PubMed: 9524818] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The cDNA sequence of proton-pumping nicotinamide nucleotide transhydrogenase from man and mouse."
Lagberg E.M., Betsholtz C., Rydstrom J.
Biochim. Biophys. Acta 1273:203-205(1996) [PubMed: 8616157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[3]The German cDNA consortium
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The high-resolution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from human heart mitochondria."
White S.A., Peake S.J., McSweeney S., Leonard G., Cotton N.P.J., Jackson J.B.
Structure 8:1-12(2000) [PubMed: 10673423] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 880-1086.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U40490 mRNA. Translation: AAC51914.1.
Z50101 mRNA. Translation: CAA90428.1.
AL831822 mRNA. Translation: CAD38536.1.
BC110543 mRNA. Translation: AAI10544.1.
PIRG02257.
RefSeqNP_036475.3.
NP_892022.2.
UniGeneHs.482043

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1DJLX-ray2.00A/B880-1086[»]
1PT9X-ray2.42A/B880-1086[»]
1U31X-ray2.20A/B880-1086[»]
ModBaseSearch...

PTM databases

PhosphoSiteQ13423.

Proteomic databases

PeptideAtlasQ13423.

Genome annotation databases

EnsemblENSG00000112992. Homo sapiens. [Contig view]
GeneID23530.
KEGGhsa:23530.

Organism-specific databases

H-InvDBHIX0019972.
HIX0032203.
HGNCHGNC:7863. NNT.
HPACAB004975.
HPA004829.
MIM607878. gene.
PharmGKBPA31667.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ13423.
HOVERGENQ13423.

Gene expression databases

ArrayExpressQ13423.
CleanExHS_NNT.
GermOnlineENSG00000112992. Homo sapiens.

Family and domain databases

InterProIPR007698. Ala_DHase/PNT_C.
IPR008142. Ala_DHase/PNT_CS1.
IPR008143. Ala_DHase/PNT_CS2.
IPR007886. Ala_DHase/PNT_N.
IPR016040. NAD(P)-bd.
IPR004571. NADP_transhyd_a.
IPR004003. NADP_transhyd_b.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF02233. PNTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00561. pntA. 1 hit.
PROSITEPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00157. NADH.
NextBio46004.
SOURCESearch...

Hide | Top

Entry information

Entry nameNNTM_HUMAN
AccessionPrimary (citable) accession number: Q13423
Secondary accession number(s): Q16796, Q2TB60, Q8N3V4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 16, 2004
Last modified: November 25, 2008
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents