NAD(P) transhydrogenase, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q13423 (NNTM_HUMAN)

Last modified June 16, 2009. Version 94. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    NAD(P) transhydrogenase, mitochondrial
    EC=1.6.1.2
Alternative name(s):
    Nicotinamide nucleotide transhydrogenase
    Pyridine nucleotide transhydrogenase

Gene names

Name:

NNT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1086 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Catalytic activity	NADPH + NAD⁺ = NADP⁺ + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side Potential.
Sequence similarities	In the N-terminal section; belongs to the AlaDH/PNT family. In the C-terminal section; belongs to the PNT beta subunit family.

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Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	oxidation reduction Ref.6 Traceable author statement. Source: UniProtKB proton transport Ref.6 Traceable author statement. Source: UniProtKB tricarboxylic acid cycle Ref.6 Traceable author statement. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain Ref.6 Traceable author statement. Source: UniProtKB
Molecular function	NAD or NADH binding Ref.6 Traceable author statement. Source: UniProtKB NAD(P)+ transhydrogenase (AB-specific) activity Inferred from electronic annotation. Source: EC NAD(P)+ transhydrogenase (B-specific) activity Ref.1 Traceable author statement. Source: UniProtKB NADP or NADPH binding Ref.6 Inferred from direct assay. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 43

Mitochondrion By similarity

Chain

44 – 1086

1043

NAD(P) transhydrogenase, mitochondrial

PRO_0000001055

Regions

Topological domain

44 – 474

431

Mitochondrial matrix

Transmembrane

475 – 493

Potential

Transmembrane

501 – 521

Potential

Transmembrane

527 – 546

Potential

Transmembrane

558 – 578

Potential

Topological domain

579 – 595

Mitochondrial matrix

Transmembrane

596 – 616

Potential

Transmembrane

622 – 642

Potential

Transmembrane

646 – 666

Potential

Transmembrane

672 – 691

Potential

Transmembrane

702 – 722

Potential

Topological domain

723 – 739

Cytoplasmic

Transmembrane

740 – 760

Potential

Transmembrane

778 – 797

Potential

Transmembrane

801 – 819

Potential

Transmembrane

833 – 853

Potential

Transmembrane

857 – 879

Potential

Topological domain

880 – 1086

207

Mitochondrial matrix

Nucleotide binding

229 – 259

NAD By similarity

Experimental info

Sequence conflict

176

A → T in CAA90428. Ref.2

Sequence conflict

212

G → E in CAD38536. Ref.3

Sequence conflict

246

A → E in AAC51914. Ref.1

Sequence conflict

262

A → S in AAC51914. Ref.1

Sequence conflict

706

F → S in AAC51914. Ref.1

Sequence conflict

731

T → P in AAC51914. Ref.1

Sequence conflict

810

S → A in CAA90428. Ref.2

Sequence conflict

824

A → P in CAA90428. Ref.2

Sequence conflict

871

I → P in AAC51914. Ref.1

Sequence conflict

929

I → F in AAC51914. Ref.1

Sequence conflict

1059

K → R in CAD38536. Ref.3

Secondary structure

1086

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q13423-1 [UniParc].

Last modified August 16, 2004. Version 3.
Checksum: 8A437658CA0EB41B
Show »

FASTA

1,086

113,896

        10         20         30         40         50         60 
MANLLKTVVT GCSCPLLSNL GSCKGLRVKK DFLRTFYTHQ ELWCKAPVKP GIPYKQLTVG 

        70         80         90        100        110        120 
VPKEIFQNEK RVALSPAGVQ NLVKQGFNVV VESGAGEASK FSDDHYRVAG AQIQGAKEVL 

       130        140        150        160        170        180 
ASDLVVKVRA PMVNPTLGVH EADLLKTSGT LISFIYPAQN PELLNKLSQR KTTVLAMDQV 

       190        200        210        220        230        240 
PRVTIAQGYD ALSSMANIAG YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL 

       250        260        270        280        290        300 
ASAGAAKSMG AIVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE 

       310        320        330        340        350        360 
MKLFAQQCKE VDILISTALI PGKKAPVLFN KEMIESMKEG SVVVDLAAEA GGNFETTKPG 

       370        380        390        400        410        420 
ELYIHKGITH IGYTDLPSRM ATQASTLYSN NITKLLKAIS PDKDNFYFDV KDDFDFGTMG 

       430        440        450        460        470        480 
HVIRGTVVMK DGKVIFPAPT PKNIPQGAPV KQKTVAELEA EKAATITPFR KTMSTASAYT 

       490        500        510        520        530        540 
AGLTGILGLG IAAPNLAFSQ MVTTFGLAGI VGYHTVWGVT PALHSPLMSV TNAISGLTAV 

       550        560        570        580        590        600 
GGLALMGGHL YPSTTSQGLA ALAAFISSVN IAGGFLVTQR MLDMFKRPTD PPEYNYLYLL 

       610        620        630        640        650        660 
PAGTFVGGYL AALYSGYNIE QIMYLGSGLC CVGALAGLST QGTARLGNAL GMIGVAGGLA 

       670        680        690        700        710        720 
ATLGVLKPGP ELLAQMSGAM ALGGTIGLTI AKRIQISDLP QLVAAFHSLV GLAAVLTCIA 

       730        740        750        760        770        780 
EYIIEYPHFA TDAAANLTKI VAYLGTYIGG VTFSGSLIAY GKLQGLLKSA PLLLPGRHLL 

       790        800        810        820        830        840 
NAGLLAASVG GIIPFMVDPS FTTGITCLGS VSALSAVMGV TLTAAIGGAD MPVVITVLNS 

       850        860        870        880        890        900 
YSGWALCAEG FLLNNNLLTI VGALIGSSGA ILSYIMCVAM NRSLANVILG GYGTTSTAGG 

       910        920        930        940        950        960 
KPMEISGTHT EINLDNAIDM IREANSIIIT PGYGLCAAKA QYPIADLVKM LTEQGKKVRF 

       970        980        990       1000       1010       1020 
GIHPVAGRMP GQLNVLLAEA GVPYDIVLEM DEINHDFPDT DLVLVIGAND TVNSAAQEDP 

      1030       1040       1050       1060       1070       1080 
NSIIAGMPVL EVWKSKQVIV MKRSLGVGYA AVDNPIFYKP NTAMLLGDAK KTCDALQAKV 


RESYQK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and deduced amino acid sequence of human nicotinamide nucleotide transhydrogenase." Ware J., Zieger B. DNA Seq. 7:369-374(1997) [PubMed: 9524818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The cDNA sequence of proton-pumping nicotinamide nucleotide transhydrogenase from man and mouse." Lagberg E.M., Betsholtz C., Rydstrom J. Biochim. Biophys. Acta 1273:203-205(1996) [PubMed: 8616157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"The high-resolution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from human heart mitochondria." White S.A., Peake S.J., McSweeney S., Leonard G., Cotton N.P.J., Jackson J.B. Structure 8:1-12(2000) [PubMed: 10673423] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 880-1086.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U40490 mRNA. Translation: AAC51914.1.
Z50101 mRNA. Translation: CAA90428.1.
AL831822 mRNA. Translation: CAD38536.1.
BC110543 mRNA. Translation: AAI10544.1.

IPI

IPI00337541.

PIR

G02257.

RefSeq

NP_036475.3.
NP_892022.2.

UniGene

Hs.482043

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DJL	X-ray	2.00	A/B	880-1086	[»]
1PT9	X-ray	2.42	A/B	880-1086	[»]
1U31	X-ray	2.20	A/B	880-1086	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q13423.

Proteomic databases

PeptideAtlas

Q13423.

PRIDE

Q13423.

Genome annotation databases

Ensembl

ENSG00000112992. Homo sapiens. [Contig view]

GeneID

23530.

KEGG

hsa:23530.

Organism-specific databases

GeneCards

GC05P043638.

H-InvDB

HIX0019972.
HIX0032203.

HGNC

HGNC:7863. NNT.

HPA

CAB004975.
HPA004829.

MIM

607878. gene.

PharmGKB

PA31667.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q13423.

HOVERGEN

Q13423.

OMA

Q13423. LAFSQMV.

Enzyme and pathway databases

BRENDA

1.6.1.2. 247.

Gene expression databases

ArrayExpress

Q13423.

Bgee

Q13423.

CleanEx

HS_NNT.

GermOnline

ENSG00000112992. Homo sapiens.

Family and domain databases

InterPro

IPR007698. Ala_DH/PNT_C.
IPR008142. Ala_DH/PNT_CS1.
IPR008143. Ala_DH/PNT_CS2.
IPR007886. Ala_DH/PNT_N.
IPR016040. NAD(P)-bd_dom.
IPR004571. NADP_transhyd_a.
IPR004003. NADP_transhyd_b.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF02233. PNTB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00561. pntA. 1 hit.

PROSITE

PS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00157. NADH.

NextBio

46004.

SOURCE

Search...

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Entry information

Entry name

NNTM_HUMAN

Accession

Primary (citable) accession number: Q13423
Secondary accession number(s): Q16796, Q2TB60, Q8N3V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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