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Reviewed, UniProtKB/Swiss-Prot Q13422 (IKZF1_HUMAN)

Last modified November 25, 2008. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DNA-binding protein Ikaros
Alternative name(s):
    Ikaros family zinc finger protein 1
    Lymphoid transcription factor LyF-1
Gene names
Name: IKZF1
Synonyms: IK1, IKAROS, LYF1, ZNFN1A1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development.

Subunit structure

Interacts with IKZF4 AND IKZF5.

Subcellular location

Nucleus.

Tissue specificity

Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

Sequence similarities

Belongs to the Ikaros C2H2-type zinc-finger protein family.

Contains 6 C2H2-type zinc fingers.

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Ontologies

Keywords

   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator

Gene Ontology (GO)

   Biological processmesoderm development Ref.2

Traceable author statement. Source: ProtInc

   Cellular componentnucleus

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionzinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform Ik1 (identifier: Q13422-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Ik2 (identifier: Q13422-2)

The sequence of this isoform differs from the canonical sequence as follows:
     54-140: Missing.
Isoform Ik3 (identifier: Q13422-3)

The sequence of this isoform differs from the canonical sequence as follows:
     197-283: Missing.
Isoform Ik4 (identifier: Q13422-4)

The sequence of this isoform differs from the canonical sequence as follows:
     10-53: Missing.
     197-283: Missing.
Isoform Ik5 (identifier: Q13422-5)

The sequence of this isoform differs from the canonical sequence as follows:
     141-283: Missing.
Isoform Ik6 (identifier: Q13422-6)

The sequence of this isoform differs from the canonical sequence as follows:
     54-283: Missing.
Isoform Ik7 (identifier: Q13422-7)

The sequence of this isoform differs from the canonical sequence as follows:
     197-238: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 519519DNA-binding protein Ikaros
PRO_0000047094

Regions

Zinc finger117 – 13923C2H2-type 1
Zinc finger145 – 16723C2H2-type 2
Zinc finger173 – 19523C2H2-type 3
Zinc finger201 – 22424C2H2-type 4
Zinc finger462 – 48423C2H2-type 5
Zinc finger490 – 51425C2H2-type 6

Natural variations

Alternative sequence10 – 5344Missing in isoform Ik4.
VSP_006847
Alternative sequence54 – 283230Missing in isoform Ik6.
VSP_006849
Alternative sequence54 – 14087Missing in isoform Ik2.
VSP_006848
Alternative sequence141 – 283143Missing in isoform Ik5.
VSP_006852
Alternative sequence197 – 28387Missing in isoform Ik3 and isoform Ik4.
VSP_006850
Alternative sequence197 – 23842Missing in isoform Ik7.
VSP_006851

Experimental info

Sequence conflict11 – 122QV → FS in AAB50683. Ref.2
Sequence conflict2141S → T in AAB50683. Ref.2
Sequence conflict2451N → K in AAB50683. Ref.2
Sequence conflict2961Missing in AAB50683. Ref.2
Sequence conflict2981S → T in AAB50683. Ref.2
Sequence conflict352 – 3554KPLA → RRS in AAB50683. Ref.2
Sequence conflict3721N → Y in AAB50683. Ref.2
Sequence conflict420 – 4267PHARNGL → RRAQRV in AAB50683. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform Ik1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7B0129C4E3FE41A8

FASTA51957,528
        10         20         30         40         50         60 
MDADEGQDMS QVSGKESPPV SDTPDEGDEP MPIPEDLSTT SGGQQSSKSD RVVASNVKVE 

        70         80         90        100        110        120 
TQSDEENGRA CEMNGEECAE DLRMLDASGE KMNGSHRDQG SSALSGVGGI RLPNGKLKCD 

       130        140        150        160        170        180 
ICGIICIGPN VLMVHKRSHT GERPFQCNQC GASFTQKGNL LRHIKLHSGE KPFKCHLCNY 

       190        200        210        220        230        240 
ACRRRDALTG HLRTHSVGKP HKCGYCGRSY KQRSSLEEHK ERCHNYLESM GLPGTLYPVI 

       250        260        270        280        290        300 
KEETNHSEMA EDLCKIGSER SLVLDRLASN VAKRKSSMPQ KFLGDKGLSD TPYDSSASYE 

       310        320        330        340        350        360 
KENEMMKSHV MDQAINNAIN YLGAESLRPL VQTPPGGSEV VPVISPMYQL HKPLAEGTPR 

       370        380        390        400        410        420 
SNHSAQDSAV ENLLLLSKAK LVPSEREASP SNSCQDSTDT ESNNEEQRSG LIYLTNHIAP 

       430        440        450        460        470        480 
HARNGLSLKE EHRAYDLLRA ASENSQDALR VVSTSGEQMK VYKCEHCRVL FLDHVMYTIH 

       490        500        510 
MGCHGFRDPF ECNMCGYHSQ DRYEFSSHIT RGEHRFHMS

« Hide

Isoform Ik2 [UniParc].

Checksum: E8E57F8BF5B306E6
Show »

43248,289
Isoform Ik3 [UniParc].

Checksum: 1093D253341A95E8
Show »

43247,634
Isoform Ik4 [UniParc].

Checksum: 137C942A5278A9F0
Show »

38843,093
Isoform Ik5 [UniParc].

Checksum: 0B060551CB3E0082
Show »

37641,229
Isoform Ik6 [UniParc].

Checksum: EB96A15185C62A18
Show »

28931,989
Isoform Ik7 [UniParc].

Checksum: B19FE76450EA40E4
Show »

47752,706

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References

« Hide 'large scale' references
[1]"Cloning and sequencing of hIk-1, a cDNA encoding a human homologue of mouse Ikaros/LyF-1."
Nietfeld W., Meyerhans A.
Immunol. Lett. 49:139-141(1996) [PubMed: 8964602] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Tissue: Bone marrow.
[2]"The Ikaros gene encodes a family of lymphocyte-restricted zinc finger DNA binding proteins, highly conserved in human and mouse."
Molnar A., Wu P., Largespada D.A., Vortkamp A., Scherer S., Copeland N.G., Jenkins N.A., Bruns G., Georgopoulos K.
J. Immunol. 156:585-592(1996) [PubMed: 8543809] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IK7).
Tissue: Lymph.
[4]"Eos and pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities."
Perdomo J., Holmes M., Chong B., Crossley M.
J. Biol. Chem. 275:38347-38354(2000) [PubMed: 10978333] [Abstract]
Cited for: INTERACTION WITH IKZF4 AND IKZF5.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U40462 mRNA. Translation: AAC50459.1.
S80876 mRNA. Translation: AAB50683.1.
BC018349 mRNA. Translation: AAH18349.1.
RefSeqNP_006051.1.
UniGeneHs.435949
Hs.488251

3D structure databases

HSSPHSSP built from PDB template 1BBO based on UniProtKB P15822.
ModBaseSearch...

Protein-protein interaction databases

IntActQ13422.

PTM databases

PhosphoSiteQ13422.

Genome annotation databases

EnsemblENSG00000185811. Homo sapiens. [Contig view]
GeneID10320.
KEGGhsa:10320.

Organism-specific databases

H-InvDBHIX0021369.
HIX0044884.
HGNCHGNC:13176. IKZF1.
HPACAB009247.
MIM603023. gene.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ13422.
HOVERGENQ13422.

Gene expression databases

ArrayExpressQ13422.
CleanExHS_IKZF1.
GermOnlineENSG00000185811. Homo sapiens.

Family and domain databases

InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
Gene3DG3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.
PfamPF00096. zf-C2H2. 3 hits.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio39123.
SOURCESearch...

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Entry information

Entry nameIKZF1_HUMAN
AccessionPrimary (citable) accession number: Q13422
Secondary accession number(s): O00598, Q8WVA3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents