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Q13416

- ORC2_HUMAN

UniProt

Q13416 - ORC2_HUMAN

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Protein

Origin recognition complex subunit 2

Gene
ORC2, ORC2L
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.2 Publications

GO - Molecular functioni

  1. DNA replication origin binding Source: ProtInc
  2. protein binding Source: UniProtKB

GO - Biological processi

  1. DNA replication Source: Reactome
  2. DNA replication initiation Source: ProtInc
  3. G1/S transition of mitotic cell cycle Source: Reactome
  4. mitotic cell cycle Source: Reactome
  5. negative regulation of transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_1095. Activation of the pre-replicative complex.
REACT_1156. Orc1 removal from chromatin.
REACT_1181. Association of licensing factors with the pre-replicative complex.
REACT_1321. E2F-enabled inhibition of pre-replication complex formation.
REACT_1707. CDC6 association with the ORC:origin complex.
REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
REACT_207. Removal of licensing factors from origins.
REACT_2243. Assembly of the pre-replicative complex.
REACT_567. Assembly of the ORC complex at the origin of replication.
REACT_6769. Activation of ATR in response to replication stress.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 2
Gene namesi
Name:ORC2
Synonyms:ORC2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:8488. ORC2.

Subcellular locationi

GO - Cellular componenti

  1. condensed chromosome inner kinetochore Source: Ensembl
  2. heterochromatin Source: Ensembl
  3. nuclear origin of replication recognition complex Source: UniProtKB
  4. nucleoplasm Source: Reactome
  5. nucleus Source: HPA
  6. origin recognition complex Source: UniProtKB
  7. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32809.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Origin recognition complex subunit 2PRO_0000127075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161Phosphothreonine2 Publications
Modified residuei122 – 1221Phosphoserine1 Publication
Modified residuei138 – 1381Phosphoserine1 Publication
Modified residuei226 – 2261Phosphothreonine1 Publication
Modified residuei280 – 2801Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ13416.
PaxDbiQ13416.
PeptideAtlasiQ13416.
PRIDEiQ13416.

PTM databases

PhosphoSiteiQ13416.

Expressioni

Gene expression databases

ArrayExpressiQ13416.
BgeeiQ13416.
CleanExiHS_ORC2L.
GenevestigatoriQ13416.

Organism-specific databases

HPAiCAB003693.
HPA034976.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4 By similarity. Interacts with MCM10. Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994592EBI-374957,EBI-374880
EBNA1P032116EBI-374957,EBI-996522From a different organism.
MCM10Q7L5905EBI-374957,EBI-374912
MCM3P252052EBI-374957,EBI-355153
ORC1Q134156EBI-374957,EBI-374847
ORC4O439296EBI-374957,EBI-374889
ORC5O439137EBI-374957,EBI-374928
TERF2Q155543EBI-374957,EBI-706637

Protein-protein interaction databases

BioGridi111041. 39 interactions.
DIPiDIP-29689N.
IntActiQ13416. 25 interactions.
MINTiMINT-1201828.
STRINGi9606.ENSP00000234296.

Structurei

3D structure databases

ProteinModelPortaliQ13416.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 100100Involved in LRWD1-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the ORC2 family.

Phylogenomic databases

eggNOGiCOG5575.
HOGENOMiHOG000045588.
HOVERGENiHBG007874.
InParanoidiQ13416.
KOiK02604.
OMAiSQMLRGE.
OrthoDBiEOG7NPFT1.
PhylomeDBiQ13416.
TreeFamiTF101092.

Family and domain databases

InterProiIPR007220. ORC2.
[Graphical view]
PANTHERiPTHR14052. PTHR14052. 1 hit.
PfamiPF04084. ORC2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q13416-1 [UniParc]FASTAAdd to Basket

« Hide

MSKPELKEDK MLEVHFVGDD DVLNHILDRE GGAKLKKERA QLLVNPKKII    50
KKPEYDLEED DQEVLKDQNY VEIMGRDVQE SLKNGSATGG GNKVYSFQNR 100
KHSEKMAKLA SELAKTPQKS VSFSLKNDPE ITINVPQSSK GHSASDKVQP 150
KNNDKSEFLS TAPRSLRKRL IVPRSHSDSE SEYSASNSED DEGVAQEHEE 200
DTNAVIFSQK IQAQNRVVSA PVGKETPSKR MKRDKTSDLV EEYFEAHSSS 250
KVLTSDRTLQ KLKRAKLDQQ TLRNLLSKVS PSFSAELKQL NQQYEKLFHK 300
WMLQLHLGFN IVLYGLGSKR DLLERFRTTM LQDSIHVVIN GFFPGISVKS 350
VLNSITEEVL DHMGTFRSIL DQLDWIVNKF KEDSSLELFL LIHNLDSQML 400
RGEKSQQIIG QLSSLHNIYL IASIDHLNAP LMWDHAKQSL FNWLWYETTT 450
YSPYTEETSY ENSLLVKQSG SLPLSSLTHV LRSLTPNARG IFRLLIKYQL 500
DNQDNPSYIG LSFQDFYQQC REAFLVNSDL TLRAQLTEFR DHKLIRTKKG 550
TDGVEYLLIP VDNGTLTDFL EKEEEEA 577
Length:577
Mass (Da):65,972
Last modified:May 30, 2000 - v2
Checksum:iDF3F9C2CF147DA5F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti106 – 1061M → K.1 Publication
Corresponds to variant rs2307361 [ dbSNP | Ensembl ].
VAR_014515
Natural varianti521 – 5211R → Q.1 Publication
Corresponds to variant rs16835624 [ dbSNP | Ensembl ].
VAR_021276

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311I → V in AAB33970. 1 Publication
Sequence conflicti236 – 2361T → L in AAB33970. 1 Publication
Sequence conflicti392 – 3921I → M in AAB33970. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40268 mRNA. Translation: AAC50326.2.
U27459 mRNA. Translation: AAB33970.1.
AY652588 Genomic DNA. Translation: AAT46690.1.
AC005037 Genomic DNA. Translation: AAY14725.1.
CH471063 Genomic DNA. Translation: EAW70228.1.
BC014834 mRNA. Translation: AAH14834.1.
CCDSiCCDS2334.1.
RefSeqiNP_006181.1. NM_006190.4.
XP_006712618.1. XM_006712555.1.
UniGeneiHs.444870.

Genome annotation databases

EnsembliENST00000234296; ENSP00000234296; ENSG00000115942.
GeneIDi4999.
KEGGihsa:4999.
UCSCiuc002uwr.3. human.

Polymorphism databases

DMDMi8488999.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40268 mRNA. Translation: AAC50326.2 .
U27459 mRNA. Translation: AAB33970.1 .
AY652588 Genomic DNA. Translation: AAT46690.1 .
AC005037 Genomic DNA. Translation: AAY14725.1 .
CH471063 Genomic DNA. Translation: EAW70228.1 .
BC014834 mRNA. Translation: AAH14834.1 .
CCDSi CCDS2334.1.
RefSeqi NP_006181.1. NM_006190.4.
XP_006712618.1. XM_006712555.1.
UniGenei Hs.444870.

3D structure databases

ProteinModelPortali Q13416.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111041. 39 interactions.
DIPi DIP-29689N.
IntActi Q13416. 25 interactions.
MINTi MINT-1201828.
STRINGi 9606.ENSP00000234296.

PTM databases

PhosphoSitei Q13416.

Polymorphism databases

DMDMi 8488999.

Proteomic databases

MaxQBi Q13416.
PaxDbi Q13416.
PeptideAtlasi Q13416.
PRIDEi Q13416.

Protocols and materials databases

DNASUi 4999.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000234296 ; ENSP00000234296 ; ENSG00000115942 .
GeneIDi 4999.
KEGGi hsa:4999.
UCSCi uc002uwr.3. human.

Organism-specific databases

CTDi 4999.
GeneCardsi GC02M201774.
HGNCi HGNC:8488. ORC2.
HPAi CAB003693.
HPA034976.
MIMi 601182. gene.
neXtProti NX_Q13416.
PharmGKBi PA32809.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5575.
HOGENOMi HOG000045588.
HOVERGENi HBG007874.
InParanoidi Q13416.
KOi K02604.
OMAi SQMLRGE.
OrthoDBi EOG7NPFT1.
PhylomeDBi Q13416.
TreeFami TF101092.

Enzyme and pathway databases

Reactomei REACT_1095. Activation of the pre-replicative complex.
REACT_1156. Orc1 removal from chromatin.
REACT_1181. Association of licensing factors with the pre-replicative complex.
REACT_1321. E2F-enabled inhibition of pre-replication complex formation.
REACT_1707. CDC6 association with the ORC:origin complex.
REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
REACT_207. Removal of licensing factors from origins.
REACT_2243. Assembly of the pre-replicative complex.
REACT_567. Assembly of the ORC complex at the origin of replication.
REACT_6769. Activation of ATR in response to replication stress.

Miscellaneous databases

GeneWikii ORC2.
ORC2L.
GenomeRNAii 4999.
NextBioi 19244.
PROi Q13416.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q13416.
Bgeei Q13416.
CleanExi HS_ORC2L.
Genevestigatori Q13416.

Family and domain databases

InterProi IPR007220. ORC2.
[Graphical view ]
PANTHERi PTHR14052. PTHR14052. 1 hit.
Pfami PF04084. ORC2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved initiator proteins in eukaryotes."
    Gavin K.A., Hidaka M., Stillman B.D.
    Science 270:1667-1671(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Hidaka M., Stillman B.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 41-42.
  3. "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L."
    Takahara K., Bong M., Brevard R., Eddy R.L., Haley L.L., Sait S.J., Shows T.B., Hoffman G.G., Greenspan D.S.
    Genomics 31:119-122(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. NIEHS SNPs program
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-106 AND GLN-521.
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  8. "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase."
    Izumi M., Yanagi K., Mizuno T., Yokoi M., Kawasaki Y., Moon K.Y., Hurwitz J., Yatagai F., Hanaoka F.
    Nucleic Acids Res. 28:4769-4777(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MCM10.
  9. "The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle."
    Ohta S., Tatsumi Y., Fujita M., Tsurimoto T., Obuse C.
    J. Biol. Chem. 278:41535-41540(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ORC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, ASSEMBLY OF THE ORC COMPLEX.
  10. "ATP-dependent assembly of the human origin recognition complex."
    Siddiqui K., Stillman B.
    J. Biol. Chem. 282:32370-32383(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RECONSTITUTION OF THE ORC COMPLEX, DISASSEMBLY OF THE ORC COMPLEX.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-116 AND SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-116; SER-122; SER-138 AND THR-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Orc2 protects ORCA from ubiquitin-mediated degradation."
    Shen Z., Prasanth S.G.
    Cell Cycle 11:3578-3589(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LRWD1.
  15. "Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing."
    Chan K.M., Zhang Z.
    J. Biol. Chem. 287:15024-15033(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BINDING TO HISTONE H3 AND H4 TRIMETHYLATION MARKS.
  16. "Dynamic association of ORCA with prereplicative complex components regulates DNA replication initiation."
    Shen Z., Chakraborty A., Jain A., Giri S., Ha T., Prasanth K.V., Prasanth S.G.
    Mol. Cell. Biol. 32:3107-3120(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LRWD1.

Entry informationi

Entry nameiORC2_HUMAN
AccessioniPrimary (citable) accession number: Q13416
Secondary accession number(s): Q13204, Q53TX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: September 3, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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