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Protein

Origin recognition complex subunit 2

Gene

ORC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.2 Publications

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: Reactome
  • DNA replication initiation Source: ProtInc
  • G1/S transition of mitotic cell cycle Source: Reactome
  • negative regulation of transcription by RNA polymerase II Source: ProtInc

Keywordsi

Biological processDNA replication

Enzyme and pathway databases

ReactomeiR-HSA-113507 E2F-enabled inhibition of pre-replication complex formation
R-HSA-176187 Activation of ATR in response to replication stress
R-HSA-68616 Assembly of the ORC complex at the origin of replication
R-HSA-68689 CDC6 association with the ORC:origin complex
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68867 Assembly of the pre-replicative complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-68962 Activation of the pre-replicative complex
SIGNORiQ13416

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 2
Gene namesi
Name:ORC2
Synonyms:ORC2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115942.8
HGNCiHGNC:8488 ORC2
MIMi601182 gene
neXtProtiNX_Q13416

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4999
OpenTargetsiENSG00000115942
PharmGKBiPA32809

Polymorphism and mutation databases

BioMutaiORC2
DMDMi8488999

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270751 – 577Origin recognition complex subunit 2Add BLAST577

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116PhosphothreonineCombined sources1
Modified residuei122PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei226PhosphothreonineCombined sources1
Modified residuei248PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13416
MaxQBiQ13416
PaxDbiQ13416
PeptideAtlasiQ13416
PRIDEiQ13416

PTM databases

iPTMnetiQ13416
PhosphoSitePlusiQ13416

Expressioni

Gene expression databases

BgeeiENSG00000115942
CleanExiHS_ORC2L
ExpressionAtlasiQ13416 baseline and differential
GenevisibleiQ13416 HS

Organism-specific databases

HPAiCAB003693
HPA073881

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (PubMed:12909626, PubMed:17716973). Interacts with DBF4 (By similarity). Interacts with MCM10 (PubMed:11095689). Interacts with LRWD1 throughout the cell cycle; this interaction, wich occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1 ubiquitination and hence stabilizes the protein (PubMed:22645314). Interacts with POLQ (PubMed:24989122).By similarity5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi111041, 63 interactors
CORUMiQ13416
DIPiDIP-29689N
ELMiQ13416
IntActiQ13416, 43 interactors
MINTiQ13416
STRINGi9606.ENSP00000234296

Structurei

Secondary structure

1577
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi474 – 481Combined sources8
Helixi486 – 501Combined sources16
Turni502 – 504Combined sources3
Helixi513 – 522Combined sources10
Helixi529 – 541Combined sources13
Beta strandi546 – 549Combined sources4
Beta strandi555 – 558Combined sources4
Helixi563 – 572Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C8HX-ray2.01A458-577[»]
5UJ8X-ray6.00E/F/G/H231-577[»]
5UJMelectron microscopy18.00B231-577[»]
ProteinModelPortaliQ13416
SMRiQ13416
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 100Involved in LRWD1-bindingAdd BLAST100

Sequence similaritiesi

Belongs to the ORC2 family.Curated

Phylogenomic databases

eggNOGiKOG2928 Eukaryota
COG5575 LUCA
GeneTreeiENSGT00390000015228
HOGENOMiHOG000045588
HOVERGENiHBG007874
InParanoidiQ13416
KOiK02604
OMAiETAFENS
OrthoDBiEOG091G0H5Q
PhylomeDBiQ13416
TreeFamiTF101092

Family and domain databases

InterProiView protein in InterPro
IPR007220 ORC2
PANTHERiPTHR14052 PTHR14052, 1 hit
PfamiView protein in Pfam
PF04084 ORC2, 1 hit

Sequencei

Sequence statusi: Complete.

Q13416-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPELKEDK MLEVHFVGDD DVLNHILDRE GGAKLKKERA QLLVNPKKII
60 70 80 90 100
KKPEYDLEED DQEVLKDQNY VEIMGRDVQE SLKNGSATGG GNKVYSFQNR
110 120 130 140 150
KHSEKMAKLA SELAKTPQKS VSFSLKNDPE ITINVPQSSK GHSASDKVQP
160 170 180 190 200
KNNDKSEFLS TAPRSLRKRL IVPRSHSDSE SEYSASNSED DEGVAQEHEE
210 220 230 240 250
DTNAVIFSQK IQAQNRVVSA PVGKETPSKR MKRDKTSDLV EEYFEAHSSS
260 270 280 290 300
KVLTSDRTLQ KLKRAKLDQQ TLRNLLSKVS PSFSAELKQL NQQYEKLFHK
310 320 330 340 350
WMLQLHLGFN IVLYGLGSKR DLLERFRTTM LQDSIHVVIN GFFPGISVKS
360 370 380 390 400
VLNSITEEVL DHMGTFRSIL DQLDWIVNKF KEDSSLELFL LIHNLDSQML
410 420 430 440 450
RGEKSQQIIG QLSSLHNIYL IASIDHLNAP LMWDHAKQSL FNWLWYETTT
460 470 480 490 500
YSPYTEETSY ENSLLVKQSG SLPLSSLTHV LRSLTPNARG IFRLLIKYQL
510 520 530 540 550
DNQDNPSYIG LSFQDFYQQC REAFLVNSDL TLRAQLTEFR DHKLIRTKKG
560 570
TDGVEYLLIP VDNGTLTDFL EKEEEEA
Length:577
Mass (Da):65,972
Last modified:May 30, 2000 - v2
Checksum:iDF3F9C2CF147DA5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131I → V in AAB33970 (PubMed:8808289).Curated1
Sequence conflicti236T → L in AAB33970 (PubMed:8808289).Curated1
Sequence conflicti392I → M in AAB33970 (PubMed:8808289).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014515106M → K1 PublicationCorresponds to variant dbSNP:rs2307361Ensembl.1
Natural variantiVAR_021276521R → Q1 PublicationCorresponds to variant dbSNP:rs16835624Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40268 mRNA Translation: AAC50326.2
U27459 mRNA Translation: AAB33970.1
AY652588 Genomic DNA Translation: AAT46690.1
AC005037 Genomic DNA Translation: AAY14725.1
CH471063 Genomic DNA Translation: EAW70228.1
BC014834 mRNA Translation: AAH14834.1
CCDSiCCDS2334.1
RefSeqiNP_006181.1, NM_006190.4
XP_006712618.1, XM_006712555.3
UniGeneiHs.444870

Genome annotation databases

EnsembliENST00000234296; ENSP00000234296; ENSG00000115942
GeneIDi4999
KEGGihsa:4999
UCSCiuc002uwr.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiORC2_HUMAN
AccessioniPrimary (citable) accession number: Q13416
Secondary accession number(s): Q13204, Q53TX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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