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Protein

Cytoplasmic dynein 1 intermediate chain 2

Gene

DYNC1I2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.

GO - Molecular functioni

  • dynein heavy chain binding Source: GO_Central
  • dynein light chain binding Source: GO_Central
  • microtubule motor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionMotor protein
Biological processHost-virus interaction, Transport

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2132295 MHC class II antigen presentation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-68877 Mitotic Prometaphase
R-HSA-8854518 AURKA Activation by TPX2
SignaLinkiQ13409

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 2
Alternative name(s):
Cytoplasmic dynein intermediate chain 2
Dynein intermediate chain 2, cytosolic
Short name:
DH IC-2
Gene namesi
Name:DYNC1I2
Synonyms:DNCI2, DNCIC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000077380.15
HGNCiHGNC:2964 DYNC1I2
MIMi603331 gene
neXtProtiNX_Q13409

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi1781
OpenTargetsiENSG00000077380
PharmGKBiPA27435

Polymorphism and mutation databases

DMDMi23503058

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001146552 – 638Cytoplasmic dynein 1 intermediate chain 2Add BLAST637

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei51PhosphoserineCombined sources1
Modified residuei90PhosphoserineBy similarity1
Modified residuei95PhosphothreonineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei104PhosphoserineCombined sources1
Isoform 2B (identifier: Q13409-2)
Modified residuei73PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Isoform 2C (identifier: Q13409-3)
Modified residuei73PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Isoform 2F (identifier: Q13409-6)
Modified residuei73PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1

Post-translational modificationi

The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13409
MaxQBiQ13409
PaxDbiQ13409
PeptideAtlasiQ13409
PRIDEiQ13409

2D gel databases

REPRODUCTION-2DPAGEiIPI00827813

PTM databases

iPTMnetiQ13409
PhosphoSitePlusiQ13409

Expressioni

Gene expression databases

BgeeiENSG00000077380
ExpressionAtlasiQ13409 baseline and differential
GenevisibleiQ13409 HS

Organism-specific databases

HPAiCAB033836
HPA040619
HPA053987

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with human adenovirus 5 hexon protein; this interaction probably allows virus intracellular transport (PubMed:20006841). Interacts with BICD2 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DYNLRB1Q9NP973EBI-742998,EBI-372128

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108119, 64 interactors
ELMiQ13409
IntActiQ13409, 28 interactors
MINTiQ13409
STRINGi9606.ENSP00000380308

Structurei

Secondary structure

1638
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi144 – 147Combined sources4
Helixi210 – 217Combined sources8
Beta strandi219 – 221Combined sources3
Helixi222 – 235Combined sources14
Turni274 – 276Combined sources3
Beta strandi289 – 293Combined sources5
Beta strandi295 – 301Combined sources7
Beta strandi306 – 308Combined sources3
Beta strandi312 – 315Combined sources4
Helixi318 – 320Combined sources3
Beta strandi326 – 330Combined sources5
Beta strandi337 – 340Combined sources4
Beta strandi347 – 351Combined sources5
Beta strandi353 – 355Combined sources3
Beta strandi358 – 361Combined sources4
Turni362 – 365Combined sources4
Beta strandi377 – 380Combined sources4
Beta strandi384 – 389Combined sources6
Turni393 – 395Combined sources3
Beta strandi398 – 402Combined sources5
Beta strandi405 – 410Combined sources6
Beta strandi419 – 423Combined sources5
Beta strandi427 – 429Combined sources3
Beta strandi447 – 449Combined sources3
Beta strandi452 – 454Combined sources3
Beta strandi456 – 460Combined sources5
Beta strandi467 – 472Combined sources6
Beta strandi477 – 483Combined sources7
Beta strandi488 – 490Combined sources3
Beta strandi497 – 506Combined sources10
Turni511 – 513Combined sources3
Beta strandi517 – 522Combined sources6
Beta strandi523 – 525Combined sources3
Beta strandi530 – 532Combined sources3
Beta strandi534 – 537Combined sources4
Beta strandi540 – 542Combined sources3
Beta strandi545 – 552Combined sources8
Turni554 – 556Combined sources3
Beta strandi558 – 560Combined sources3
Beta strandi568 – 570Combined sources3
Beta strandi575 – 578Combined sources4
Beta strandi585 – 588Combined sources4
Beta strandi590 – 592Combined sources3
Beta strandi594 – 597Combined sources4
Beta strandi600 – 602Combined sources3
Helixi610 – 619Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JPWNMR-A/B134-154[»]
6F1Telectron microscopy3.50g/h/o/p1-638[»]
6F1Uelectron microscopy3.40h1-638[»]
6F1Zelectron microscopy3.40o/p1-638[»]
6F38electron microscopy6.70g/h/o/p1-638[»]
6F3Aelectron microscopy8.20g/h1-638[»]
ProteinModelPortaliQ13409
SMRiQ13409
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati277 – 326WD 1Add BLAST50
Repeati330 – 370WD 2Add BLAST41
Repeati379 – 420WD 3Add BLAST42
Repeati429 – 469WD 4Add BLAST41
Repeati474 – 519WD 5Add BLAST46
Repeati522 – 562WD 6Add BLAST41
Repeati568 – 607WD 7Add BLAST40

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1587 Eukaryota
ENOG410XQ99 LUCA
GeneTreeiENSGT00730000110228
HOVERGENiHBG004083
InParanoidiQ13409
KOiK10415
OMAiQFYDERW
OrthoDBiEOG091G038G
PhylomeDBiQ13409
TreeFamiTF300553

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR025956 DYNC1I1/DYNC1I2
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF11540 Dynein_IC2, 1 hit
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2A (identifier: Q13409-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDKSELKAE LERKKQRLAQ IREEKKRKEE ERKKKETDQK KEAVAPVQEE
60 70 80 90 100
SDLEKKRREA EALLQSMGLT PESPIVFSEY WVPPPMSPSS KSVSTPSEAG
110 120 130 140 150
SQDSGDGAVG SRTLHWDTDP SVLQLHSDSD LGRGPIKLGM AKITQVDFPP
160 170 180 190 200
REIVTYTKET QTPVMAQPKE DEEEDDDVVA PKPPIEPEEE KTLKKDEEND
210 220 230 240 250
SKAPPHELTE EEKQQILHSE EFLSFFDHST RIVERALSEQ INIFFDYSGR
260 270 280 290 300
DLEDKEGEIQ AGAKLSLNRQ FFDERWSKHR VVSCLDWSSQ YPELLVASYN
310 320 330 340 350
NNEDAPHEPD GVALVWNMKY KKTTPEYVFH CQSAVMSATF AKFHPNLVVG
360 370 380 390 400
GTYSGQIVLW DNRSNKRTPV QRTPLSAAAH THPVYCVNVV GTQNAHNLIS
410 420 430 440 450
ISTDGKICSW SLDMLSHPQD SMELVHKQSK AVAVTSMSFP VGDVNNFVVG
460 470 480 490 500
SEEGSVYTAC RHGSKAGISE MFEGHQGPIT GIHCHAAVGA VDFSHLFVTS
510 520 530 540 550
SFDWTVKLWT TKNNKPLYSF EDNADYVYDV MWSPTHPALF ACVDGMGRLD
560 570 580 590 600
LWNLNNDTEV PTASISVEGN PALNRVRWTH SGREIAVGDS EGQIVIYDVG
610 620 630
EQIAVPRNDE WARFGRTLAE INANRADAEE EAATRIPA
Length:638
Mass (Da):71,457
Last modified:September 19, 2002 - v3
Checksum:i2F868EC9556C47F2
GO
Isoform 2B (identifier: Q13409-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.

Show »
Length:632
Mass (Da):70,645
Checksum:i5E643B54A2979294
GO
Isoform 2C (identifier: Q13409-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.
     113-132: Missing.

Show »
Length:612
Mass (Da):68,426
Checksum:i518E1D765A2CBB15
GO
Isoform 2E (identifier: Q13409-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     602-602: Missing.

Show »
Length:637
Mass (Da):71,328
Checksum:i0526BC20523DF804
GO
Isoform 2F (identifier: Q13409-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.
     113-132: Missing.
     602-602: Missing.

Show »
Length:611
Mass (Da):68,298
Checksum:i9DB5032F12C11F5B
GO
Isoform 3 (identifier: Q13409-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-81: VFSEYW → AEQPLRVVTADTCLFHYL
     113-132: Missing.

Note: No experimental confirmation available.
Show »
Length:630
Mass (Da):70,485
Checksum:i5C3022F6F62D9C67
GO

Sequence cautioni

The sequence AAA89166 differs from that shown. Unlikely isoform. Several sequence problems.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti510T → S in AAP97254 (Ref. 1) Curated1
Sequence conflicti525D → G in AAP97254 (Ref. 1) Curated1
Sequence conflicti572A → G in AAI09376 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05437776 – 81VFSEYW → AEQPLRVVTADTCLFHYL in isoform 3. 1 Publication6
Alternative sequenceiVSP_00133677 – 82Missing in isoform 2B, isoform 2C and isoform 2F. 5 Publications6
Alternative sequenceiVSP_001337113 – 132Missing in isoform 2C, isoform 2F and isoform 3. 6 PublicationsAdd BLAST20
Alternative sequenceiVSP_023011602Missing in isoform 2E and isoform 2F. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF134477 mRNA Translation: AAP97254.1
AF250307 mRNA Translation: AAK37426.1
AY037160 mRNA Translation: AAK67638.1
BT007130 mRNA Translation: AAP35794.1
AK055491 mRNA Translation: BAB70932.1
AK316119 mRNA Translation: BAH14490.1
AL137519 mRNA Translation: CAB70785.1
BX537412 mRNA Translation: CAD97654.1
AC064826 Genomic DNA No translation available.
AC068039 Genomic DNA Translation: AAY24133.1
CH471058 Genomic DNA Translation: EAX11202.1
CH471058 Genomic DNA Translation: EAX11204.1
CH471058 Genomic DNA Translation: EAX11206.1
CH471058 Genomic DNA Translation: EAX11207.1
CH471058 Genomic DNA Translation: EAX11208.1
CH471058 Genomic DNA Translation: EAX11209.1
BC091498 mRNA Translation: AAH91498.1
BC109375 mRNA Translation: AAI09376.1
BC015038 mRNA Translation: AAH15038.1
U39575 mRNA Translation: AAA89166.1 Sequence problems.
CCDSiCCDS46450.1 [Q13409-1]
CCDS63054.1 [Q13409-7]
CCDS63056.1 [Q13409-3]
CCDS63057.1 [Q13409-6]
CCDS82533.1 [Q13409-2]
PIRiT46365
RefSeqiNP_001258714.1, NM_001271785.1 [Q13409-1]
NP_001258715.1, NM_001271786.1 [Q13409-7]
NP_001258716.1, NM_001271787.1 [Q13409-7]
NP_001258717.1, NM_001271788.1 [Q13409-3]
NP_001258718.1, NM_001271789.1 [Q13409-3]
NP_001258719.1, NM_001271790.1 [Q13409-6]
NP_001307811.1, NM_001320882.1 [Q13409-2]
NP_001307812.1, NM_001320883.1 [Q13409-2]
NP_001307813.1, NM_001320884.1 [Q13409-6]
NP_001369.1, NM_001378.2 [Q13409-1]
UniGeneiHs.546250

Genome annotation databases

EnsembliENST00000340296; ENSP00000339430; ENSG00000077380 [Q13409-3]
ENST00000397119; ENSP00000380308; ENSG00000077380 [Q13409-1]
ENST00000409197; ENSP00000386397; ENSG00000077380 [Q13409-3]
ENST00000409317; ENSP00000386591; ENSG00000077380 [Q13409-2]
ENST00000409453; ENSP00000386886; ENSG00000077380 [Q13409-5]
ENST00000409773; ENSP00000386415; ENSG00000077380 [Q13409-1]
ENST00000410079; ENSP00000386522; ENSG00000077380 [Q13409-7]
ENST00000508530; ENSP00000423339; ENSG00000077380 [Q13409-6]
GeneIDi1781
KEGGihsa:1781
UCSCiuc002uha.3 human [Q13409-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDC1I2_HUMAN
AccessioniPrimary (citable) accession number: Q13409
Secondary accession number(s): B7ZA04
, D3DPD4, D3DPD5, D3DPD6, Q32LY9, Q53S84, Q5BJF8, Q7Z4X1, Q96NG7, Q96S87, Q9BXZ5, Q9NT58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 19, 2002
Last modified: April 25, 2018
This is version 186 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health