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Reviewed, UniProtKB/Swiss-Prot Q13409 (DC1I2_HUMAN)

Last modified November 24, 2009. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cytoplasmic dynein 1 intermediate chain 2
Alternative name(s):
    Cytoplasmic dynein intermediate chain 2
    Dynein intermediate chain 2, cytosolic
    DH IC-2
Gene names
Name: DYNC1I2
Synonyms: DNCI2, DNCIC2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

The intermediate chains seem to help dynein bind to dynactin 150 kDa component.

Subunit structure

Consists of at least two heavy chains and a number of intermediate and light chains.

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.11 Ref.12 Ref.13 Ref.14

Sequence similarities

Belongs to the dynein intermediate chain family.

Contains 7 WD repeats.

Sequence caution

The sequence AAA89166.1 differs from that shown. Reason: Miscellaneous discrepancy. Unlikely isoform. Several sequence problems.

Ontologies

Keywords
   Cellular componentDynein
Microtubule
   Coding sequence diversityAlternative splicing
   DomainRepeat
WD repeat
   Molecular functionMotor protein
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processmicrotubule-based movement Ref.9

Non-traceable author statement. Source: UniProtKB

   Cellular componentcytosol

Inferred from Experiment. Source: Reactome

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionmicrotubule motor activity Ref.9

Non-traceable author statement. Source: UniProtKB

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DYNLL1P631671EBI-742998,EBI-349105

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2A (identifier: Q13409-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2B (identifier: Q13409-2)

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.
Isoform 2C (identifier: Q13409-3)

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.
     113-132: Missing.
Isoform 2E (identifier: Q13409-5)

The sequence of this isoform differs from the canonical sequence as follows:
     602-602: Missing.
Isoform 2F (identifier: Q13409-6)

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.
     113-132: Missing.
     602-602: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 638638Cytoplasmic dynein 1 intermediate chain 2
PRO_0000114655

Regions

Repeat277 – 32650WD 1
Repeat330 – 37041WD 2
Repeat379 – 42042WD 3
Repeat429 – 46941WD 4
Repeat474 – 51946WD 5
Repeat522 – 56241WD 6
Repeat568 – 60740WD 7

Amino acid modifications

Modified residue871Phosphoserine Ref.11
Modified residue921Phosphoserine
Modified residue941Phosphoserine Ref.12 Ref.14
Modified residue951Phosphothreonine
Modified residue971Phosphoserine Ref.13 Ref.14
Modified residue1011Phosphoserine Ref.13
Modified residue1041Phosphoserine
Modified residue1621Phosphothreonine

Natural variations

Alternative sequence77 – 826Missing in isoform 2B, isoform 2C and isoform 2F.
VSP_001336
Alternative sequence113 – 13220Missing in isoform 2C and isoform 2F.
VSP_001337
Alternative sequence6021Missing in isoform 2E and isoform 2F.
VSP_023011

Experimental info

Sequence conflict5101T → S in AAP97254. Ref.1
Sequence conflict5251D → G in AAP97254. Ref.1
Sequence conflict5721A → G in AAI09376. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform 2A [UniParc].

Last modified September 19, 2002. Version 3.
Checksum: 2F868EC9556C47F2

FASTA63871,457
        10         20         30         40         50         60 
MSDKSELKAE LERKKQRLAQ IREEKKRKEE ERKKKETDQK KEAVAPVQEE SDLEKKRREA 

        70         80         90        100        110        120 
EALLQSMGLT PESPIVFSEY WVPPPMSPSS KSVSTPSEAG SQDSGDGAVG SRTLHWDTDP 

       130        140        150        160        170        180 
SVLQLHSDSD LGRGPIKLGM AKITQVDFPP REIVTYTKET QTPVMAQPKE DEEEDDDVVA 

       190        200        210        220        230        240 
PKPPIEPEEE KTLKKDEEND SKAPPHELTE EEKQQILHSE EFLSFFDHST RIVERALSEQ 

       250        260        270        280        290        300 
INIFFDYSGR DLEDKEGEIQ AGAKLSLNRQ FFDERWSKHR VVSCLDWSSQ YPELLVASYN 

       310        320        330        340        350        360 
NNEDAPHEPD GVALVWNMKY KKTTPEYVFH CQSAVMSATF AKFHPNLVVG GTYSGQIVLW 

       370        380        390        400        410        420 
DNRSNKRTPV QRTPLSAAAH THPVYCVNVV GTQNAHNLIS ISTDGKICSW SLDMLSHPQD 

       430        440        450        460        470        480 
SMELVHKQSK AVAVTSMSFP VGDVNNFVVG SEEGSVYTAC RHGSKAGISE MFEGHQGPIT 

       490        500        510        520        530        540 
GIHCHAAVGA VDFSHLFVTS SFDWTVKLWT TKNNKPLYSF EDNADYVYDV MWSPTHPALF 

       550        560        570        580        590        600 
ACVDGMGRLD LWNLNNDTEV PTASISVEGN PALNRVRWTH SGREIAVGDS EGQIVIYDVG 

       610        620        630 
EQIAVPRNDE WARFGRTLAE INANRADAEE EAATRIPA 

« Hide

Isoform 2B.

Checksum: 5E643B54A2979294
Show »

FASTA63270,645
Isoform 2C.

Checksum: 518E1D765A2CBB15
Show »

FASTA61268,426
Isoform 2E.

Checksum: 0526BC20523DF804
Show »

FASTA63771,328
Isoform 2F.

Checksum: 9DB5032F12C11F5B
Show »

FASTA61168,298

References

« Hide 'large scale' references
[1]"Cloning of a new human cDNA homologous to Rattus norvegicus cytoplasmic dynein intermediate chain 2C."
Huang J., Yu L., Zhao S.Y.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2C).
[2]Li N., Wan T., Zhang M., Zhang W., Cao X.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2C AND 2F).
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2C).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2A).
Tissue: Brain.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2C).
Tissue: Endometrium and Testis.
[6]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed: 15815621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2C).
Tissue: Kidney, Skin and Testis.
[9]"Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued."
Vaughan K.T., Vallee R.B.
J. Cell Biol. 131:1507-1516(1995) [PubMed: 8522607] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-448 (ISOFORM 2A).
[10]Lubec G., Afjehi-Sadat L.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 236-250, MASS SPECTROMETRY.
Tissue: Brain and Cajal-Retzius cell.
[11]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87, MASS SPECTROMETRY.
Tissue: Epithelium.
[12]"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry."
Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.
Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, MASS SPECTROMETRY.
[13]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND SER-101, MASS SPECTROMETRY.
[14]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94 AND SER-97, MASS SPECTROMETRY.
[15]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92; SER-94; THR-95; SER-101; SER-104 AND THR-162, MASS SPECTROMETRY.
[16]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-104, MASS SPECTROMETRY.
Tissue: T-cell.

Cross-references

Sequence databases

AF134477 mRNA. Translation: AAP97254.1.
AF250307 mRNA. Translation: AAK37426.1.
AY037160 mRNA. Translation: AAK67638.1.
BT007130 mRNA. Translation: AAP35794.1.
AK055491 mRNA. Translation: BAB70932.1.
AL137519 mRNA. Translation: CAB70785.1.
BX537412 mRNA. Translation: CAD97654.1.
AC064826 Genomic DNA. No translation available.
AC068039 Genomic DNA. Translation: AAY24133.1.
CH471058 Genomic DNA. Translation: EAX11202.1.
BC091498 mRNA. Translation: AAH91498.1.
BC109375 mRNA. Translation: AAI09376.1.
BC015038 mRNA. Translation: AAH15038.1.
U39575 mRNA. Translation: AAA89166.1. Sequence problems.
IPIIPI00216348.
IPI00302712.
IPI00744015.
IPI00827813.
IPI00827859.
PIRT46365.
RefSeqNP_001369.1.
UniGeneHs.546250

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ13409. 2 interactions.
STRINGQ13409.

PTM databases

PhosphoSiteQ13409.

2-D gel databases

REPRODUCTION-2DPAGEIPI00827813.

Proteomic databases

PRIDEQ13409.

Genome annotation databases

EnsemblENST00000397119; ENSP00000380308; ENSG00000077380; Homo sapiens. [Genome view]
ENST00000409773; ENSP00000386415; ENSG00000077380; Homo sapiens. [Genome view]
GeneID1781.
KEGGhsa:1781.
UCSCuc002uha.1. human.
uc002uhc.2. human.

Organism-specific databases

CTD1781.
GeneCardsGC02P172252.
H-InvDBHIX0002589.
HGNCHGNC:2964. DYNC1I2.
MIM603331. gene.
PharmGKBPA27435.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ13409.
HOVERGENQ13409.
OMAVAVTCMS

Enzyme and pathway databases

ReactomeREACT_152. Cell Cycle, Mitotic.

Gene expression databases

ArrayExpressQ13409.
BgeeQ13409.
GenevestigatorQ13409.
GermOnlineENSG00000077380. Homo sapiens.

Family and domain databases

InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR017986. WD40_repeat_dom.
IPR019781. WD40_repeat_sg.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 5 hits.
[Graphical view]
PROSITEPS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. False negative.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio7247.
SOURCESearch...

Entry information

Entry nameDC1I2_HUMAN
AccessionPrimary (citable) accession number: Q13409
Secondary accession number(s): Q32LY9 expand/collapse secondary AC list , Q53S84, Q5BJF8, Q7Z4X1, Q96NG7, Q96S87, Q9BXZ5, Q9NT58
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 19, 2002
Last modified: November 24, 2009
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents