Q13409 (DC1I2_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 123.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Cytoplasmic dynein 1 intermediate chain 2 Alternative name(s): Cytoplasmic dynein intermediate chain 2 Dynein intermediate chain 2, cytosolic Short name=DH IC-2 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 638 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes. |
| Subunit structure | Homodimer By similarity. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 By similarity. Interacts with human adenovirus 5 hexon protein; this interaction probably allows virus intracellular transport. Ref.17 |
| Subcellular location | Cytoplasm › cytoskeleton By similarity. |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR. The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding By similarity. Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 |
| Sequence similarities | Belongs to the dynein intermediate chain family. Contains 7 WD repeats. |
| Sequence caution | The sequence AAA89166.1 differs from that shown. Reason: Unlikely isoform. Several sequence problems. |
Ontologies
Alternative products
| This entry describes 5 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 2A (identifier: Q13409-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2B (identifier: Q13409-2) The sequence of this isoform differs from the canonical sequence as follows: 77-82: Missing. | ||||||
| Isoform 2C (identifier: Q13409-3) The sequence of this isoform differs from the canonical sequence as follows: 77-82: Missing. 113-132: Missing. | ||||||
| Isoform 2E (identifier: Q13409-5) The sequence of this isoform differs from the canonical sequence as follows: 602-602: Missing. | ||||||
| Isoform 2F (identifier: Q13409-6) The sequence of this isoform differs from the canonical sequence as follows: 77-82: Missing. 113-132: Missing. 602-602: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 638 | 638 | Cytoplasmic dynein 1 intermediate chain 2 | PRO_0000114655 | |||||
Regions | |||||||||
| Repeat | 277 – 326 | 50 | WD 1 | ||||||
| Repeat | 330 – 370 | 41 | WD 2 | ||||||
| Repeat | 379 – 420 | 42 | WD 3 | ||||||
| Repeat | 429 – 469 | 41 | WD 4 | ||||||
| Repeat | 474 – 519 | 46 | WD 5 | ||||||
| Repeat | 522 – 562 | 41 | WD 6 | ||||||
| Repeat | 568 – 607 | 40 | WD 7 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 87 | 1 | Phosphoserine Ref.11 Ref.16 | ||||||
| Modified residue | 90 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 92 | 1 | Phosphoserine Ref.15 | ||||||
| Modified residue | 94 | 1 | Phosphoserine Ref.12 Ref.14 Ref.15 | ||||||
| Modified residue | 95 | 1 | Phosphothreonine Ref.15 | ||||||
| Modified residue | 97 | 1 | Phosphoserine Ref.13 Ref.14 | ||||||
| Modified residue | 101 | 1 | Phosphoserine Ref.13 Ref.15 | ||||||
| Modified residue | 104 | 1 | Phosphoserine Ref.15 Ref.16 | ||||||
| Modified residue | 162 | 1 | Phosphothreonine Ref.15 | ||||||
Natural variations | |||||||||
| Alternative sequence | 77 – 82 | 6 | Missing in isoform 2B, isoform 2C and isoform 2F. | VSP_001336 | |||||
| Alternative sequence | 113 – 132 | 20 | Missing in isoform 2C and isoform 2F. | VSP_001337 | |||||
| Alternative sequence | 602 | 1 | Missing in isoform 2E and isoform 2F. | VSP_023011 | |||||
Experimental info | |||||||||
| Sequence conflict | 510 | 1 | T → S in AAP97254. Ref.1 | ||||||
| Sequence conflict | 525 | 1 | D → G in AAP97254. Ref.1 | ||||||
| Sequence conflict | 572 | 1 | A → G in AAI09376. Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning of a new human cDNA homologous to Rattus norvegicus cytoplasmic dynein intermediate chain 2C." Huang J., Yu L., Zhao S.Y. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2C). |
| [2] | Li N., Wan T., Zhang M., Zhang W., Cao X. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2C AND 2F). |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2C). |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2A). Tissue: Brain. |
| [5] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2C). Tissue: Endometrium and Testis. |
| [6] | "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. Wilson R.K.Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [8] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2C). Tissue: Kidney, Skin and Testis. |
| [9] | "Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued." Vaughan K.T., Vallee R.B. J. Cell Biol. 131:1507-1516(1995) [PubMed: 8522607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-448 (ISOFORM 2A). |
| [10] | Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 236-250, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell. |
| [11] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [12] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [13] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND SER-101, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [14] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94 AND SER-97, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [15] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92; SER-94; THR-95; SER-101; SER-104 AND THR-162, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [16] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-104, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| [17] | "Adenovirus transport via direct interaction of cytoplasmic dynein with the viral capsid hexon subunit." Bremner K.H., Scherer J., Yi J., Vershinin M., Gross S.P., Vallee R.B. Cell Host Microbe 6:523-535(2009) [PubMed: 20006841] [Abstract] Cited for: INTERACTION WITH HUMAN ADENOVIRUS HEXON PROTEIN. |
| [18] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF134477 mRNA. Translation: AAP97254.1. AF250307 mRNA. Translation: AAK37426.1. AY037160 mRNA. Translation: AAK67638.1. BT007130 mRNA. Translation: AAP35794.1. AK055491 mRNA. Translation: BAB70932.1. AL137519 mRNA. Translation: CAB70785.1. BX537412 mRNA. Translation: CAD97654.1. AC064826 Genomic DNA. No translation available. AC068039 Genomic DNA. Translation: AAY24133.1. CH471058 Genomic DNA. Translation: EAX11202.1. CH471058 Genomic DNA. Translation: EAX11204.1. CH471058 Genomic DNA. Translation: EAX11206.1. CH471058 Genomic DNA. Translation: EAX11207.1. CH471058 Genomic DNA. Translation: EAX11208.1. CH471058 Genomic DNA. Translation: EAX11209.1. BC091498 mRNA. Translation: AAH91498.1. BC109375 mRNA. Translation: AAI09376.1. BC015038 mRNA. Translation: AAH15038.1. U39575 mRNA. Translation: AAA89166.1. Sequence problems. |
| IPI | IPI00216348. IPI00302712. IPI00744015. IPI00827813. IPI00827859. |
| PIR | T46365. |
| RefSeq | NP_001369.1. NM_001378.1. |
| UniGene | Hs.546250. |
3D structure databases | |
| ProteinModelPortal | Q13409. |
| SMR | Q13409. Positions 136-164, 208-245, 468-602. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q13409. 4 interactions. |
| MINT | MINT-5004520. |
| STRING | Q13409. |
PTM databases | |
| PhosphoSite | Q13409. |
Polymorphism databases | |
| DMDM | 23503058. |
2D gel databases | |
| REPRODUCTION-2DPAGE | IPI00827813. |
Proteomic databases | |
| PRIDE | Q13409. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000397119; ENSP00000380308; ENSG00000077380. ENST00000409773; ENSP00000386415; ENSG00000077380. |
| GeneID | 1781. |
| KEGG | hsa:1781. |
| UCSC | uc002uha.1. human. uc002uhc.2. human. |
Organism-specific databases | |
| CTD | 1781. |
| GeneCards | GC02P172543. |
| H-InvDB | HIX0002589. |
| HGNC | HGNC:2964. DYNC1I2. |
| HPA | CAB033836. |
| MIM | 603331. gene. |
| neXtProt | NX_Q13409. |
| PharmGKB | PA27435. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG09255. |
| HOVERGEN | HBG004083. |
| OMA | IVFSEYW. |
| OrthoDB | EOG4RBQJ5. |
| PhylomeDB | Q13409. |
Enzyme and pathway databases | |
| Reactome | REACT_152. Cell Cycle, Mitotic. |
Gene expression databases | |
| ArrayExpress | Q13409. |
| Bgee | Q13409. |
| Genevestigator | Q13409. |
| GermOnline | ENSG00000077380. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR017986. WD40_repeat_dom. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| KO | K10415. |
| Pfam | PF00400. WD40. 2 hits. [Graphical view] |
| SMART | SM00320. WD40. 5 hits. [Graphical view] |
| SUPFAM | SSF50978. WD40_like. 1 hit. |
| PROSITE | PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 7247. |
| SOURCE | Search... |
Entry information
| Entry name | DC1I2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q13409 Secondary accession number(s): D3DPD4 Q9NT58 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with