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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Rhodopseudomonas palustris (strain BisB5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei399 – 3991NucleophileUniRule annotation
Active sitei452 – 4521Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciRPAL316057:GHDC-3544-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:RPD_3484
OrganismiRhodopseudomonas palustris (strain BisB5)
Taxonomic identifieri316057 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7167161,4-alpha-glucan branching enzyme GlgBPRO_0000260690Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi316057.RPD_3484.

Structurei

3D structure databases

ProteinModelPortaliQ133N2.
SMRiQ133N2. Positions 112-715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q133N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVQLTDEAY AVLEGRHADP FHYLGPHSED NRVVVRALLP DATAVEAVGE
60 70 80 90 100
HGETAHLERV HESGLFAGSL PNGSHRYQLR ARFGDTTVDL EDPYRFPPIL
110 120 130 140 150
TDFDLYLLGE GTDQRLYDKL GAHPMALEGV NGVAFVVLAP NARRVSVVGD
160 170 180 190 200
FNFWNPRRHQ MRVRGNGYWE LFVPGAAAGD HYKFDVVGPH GEQLPQKSDP
210 220 230 240 250
MAFAAELRPK TASIVVDQTR LPQPRPAPAQ INALGAPMSI YEVHLGSWRR
260 270 280 290 300
KDGEQWLSYR ELAETLPAYV REMGFTHVEF LPVSEHPFDG SWGYQPTGLY
310 320 330 340 350
APTSRFGTPE DFCALIDAFH AQGIGVLLDW VPGHFPDDPH GLGHFDGTAL
360 370 380 390 400
YEHANPLQGR HLDWGTLIYN YGRTEVVNFL VSNALFWLER YGIDGLRVDA
410 420 430 440 450
VASMLYLDYS RPADGWVPNK FGGRENIEAI DFLRRFNAEV FAKFPHVTTA
460 470 480 490 500
AEESTAWPQV SRPVEFGGLG FGYKWNMGWM HDTLNYISKD PIHRKFHHGQ
510 520 530 540 550
ILFGLHYAFS ENFILPLSHD EVVHGKRSIL GRMPGDEWQR FANLRAYYAF
560 570 580 590 600
MFAHPGKKLM FMGSEFGQER EWNHDRSLDW HLLDTPKYAG IQALVRDLNR
610 620 630 640 650
LYRTLPALHQ LDCDPFGFEW LVTDDADRNV FAWMRKGDDP RARCLVIANF
660 670 680 690 700
SPNVYEDYRV RVPFPGRWRE ALNSDSAVYG GSNVGNAGEV HTLDGLVPEL
710
SLTIPPLAAI FLTPED
Length:716
Mass (Da):80,980
Last modified:October 31, 2006 - v1
Checksum:i27CA7CB7801A4B1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000283 Genomic DNA. Translation: ABE40707.1.
RefSeqiWP_011503874.1. NC_007958.1.
YP_570608.1. NC_007958.1.

Genome annotation databases

EnsemblBacteriaiABE40707; ABE40707; RPD_3484.
KEGGirpd:RPD_3484.
PATRICi23282103. VBIRhoPal120395_3607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000283 Genomic DNA. Translation: ABE40707.1.
RefSeqiWP_011503874.1. NC_007958.1.
YP_570608.1. NC_007958.1.

3D structure databases

ProteinModelPortaliQ133N2.
SMRiQ133N2. Positions 112-715.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316057.RPD_3484.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE40707; ABE40707; RPD_3484.
KEGGirpd:RPD_3484.
PATRICi23282103. VBIRhoPal120395_3607.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciRPAL316057:GHDC-3544-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BisB5.

Entry informationi

Entry nameiGLGB_RHOPS
AccessioniPrimary (citable) accession number: Q133N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 31, 2006
Last modified: April 29, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.