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Q13371 (PHLP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosducin-like protein

Short name=PHLP
Gene names
Name:PDCL
Synonyms:PHLOP1, PhLP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 1:Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Ref.12 Ref.14

Isoform 2:Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin. Ref.12 Ref.14

Subunit structure

Forms a complex with the beta and gamma subunits of the GTP-binding protein, transducin. Interacts with the CCT chaperonin complex. Ref.12 Ref.14

Sequence similarities

Belongs to the phosducin family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q13371-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q13371-2)

Also known as: PhLPs;

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.
Note: Expressed ubiquitously, highest levels are found in neural tissues amounting to 10% of total PDCL mRNA.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 301300Phosducin-like protein
PRO_0000163755

Amino acid modifications

Modified residue21N-acetylthreonine Ref.7
Modified residue251Phosphoserine By similarity
Modified residue2931Phosphoserine Ref.11
Modified residue2961Phosphoserine Ref.9 Ref.11

Natural variations

Alternative sequence1 – 8383Missing in isoform 2.
VSP_053571
Natural variant2181K → N.
Corresponds to variant rs4466466 [ dbSNP | Ensembl ].
VAR_050525

Experimental info

Sequence conflict1511Q → E in AAD01930. Ref.1
Sequence conflict2181K → E in CAB56011. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 17, 2003. Version 3.
Checksum: 717675FF869760D5

FASTA30134,282
        10         20         30         40         50         60 
MTTLDDKLLG EKLQYYYSSS EDEDSDHEDK DRGRCAPASS SVPAEAELAG EGISVNTGPK 

        70         80         90        100        110        120 
GVINDWRRFK QLETEQREEQ CREMERLIKK LSMTCRSHLD EEEEQQKQKD LQEKISGKMT 

       130        140        150        160        170        180 
LKEFAIMNED QDDEEFLQQY RKQRMEEMRQ QLHKGPQFKQ VFEISSGEGF LDMIDKEQKS 

       190        200        210        220        230        240 
IVIMVHIYED GIPGTEAMNG CMICLAAEYP AVKFCKVKSS VIGASSQFTR NALPALLIYK 

       250        260        270        280        290        300 
GGELIGNFVR VTDQLGDDFF AVDLEAFLQE FGLLPEKEVL VLTSVRNSAT CHSEDSDLEI 


D 

« Hide

Isoform 2 (PhLPs) [UniParc].

Checksum: 5B7D4109656A481B
Show »

FASTA21824,902

References

« Hide 'large scale' references
[1]"Molecular cloning and functional analysis of human phosducin-like protein."
Lazarov M.E., Martin M.M., Savage J.R., Willardson B.M., Elton T.S.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Cerebellum.
[4]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Ovary.
[7]Bienvenut W.V., Waridel P., Quadroni M.
Submitted (MAR-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-12 AND 278-286, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryonic kidney.
[8]"Cloning and characterization of the rat and human phosducin-like protein genes: structure, expression and chromosomal localization."
Thibault C., Wang J.-F., Charnas R., Mirel D., Barhite S., Miles M.F.
Biochim. Biophys. Acta 1444:346-354(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-264 (ISOFORM 1).
[9]"Identification of phosphorylation sites on phosducin-like protein by QTOF mass spectrometry."
Carter M.D., Southwick K., Lukov G., Willardson B.M., Thulin C.D.
J. Biomol. Tech. 15:257-264(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-296.
[10]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-293 AND SER-296, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity."
Howlett A.C., Gray A.J., Hunter J.M., Willardson B.M.
J. Biol. Chem. 284:16386-16399(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION (ISOFORM 1), SUBUNIT.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins."
Gao X., Sinha S., Belcastro M., Woodard C., Ramamurthy V., Stoilov P., Sokolov M.
J. Biol. Chem. 288:25760-25768(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031463 mRNA. Translation: AAD01930.1.
AL117602 mRNA. Translation: CAB56011.1.
AK313429 mRNA. Translation: BAG36221.1.
AL359512 Genomic DNA. Translation: CAI94958.1.
CH471090 Genomic DNA. Translation: EAW87546.1.
BC017227 mRNA. Translation: AAH17227.1.
AF083324 Genomic DNA. Translation: AAC78848.1.
AF083325 Genomic DNA. Translation: AAC78849.1.
U38236 Genomic DNA. Translation: AAA79724.1.
CCDSCCDS6845.1.
PIRT17321.
RefSeqNP_005379.3. NM_005388.4. [Q13371-1]
UniGeneHs.271749.
Hs.620774.

3D structure databases

ProteinModelPortalQ13371.
SMRQ13371. Positions 51-277.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111116. 11 interactions.
IntActQ13371. 2 interactions.
MINTMINT-2806359.
STRING9606.ENSP00000259467.

PTM databases

PhosphoSiteQ13371.

Proteomic databases

MaxQBQ13371.
PaxDbQ13371.
PRIDEQ13371.

Protocols and materials databases

DNASU5082.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000259467; ENSP00000259467; ENSG00000136940. [Q13371-1]
GeneID5082.
KEGGhsa:5082.
UCSCuc004bmz.2. human. [Q13371-1]

Organism-specific databases

CTD5082.
GeneCardsGC09M125560.
HGNCHGNC:8770. PDCL.
HPACAB026473.
HPA021571.
HPA021647.
MIM604421. gene.
neXtProtNX_Q13371.
PharmGKBPA33119.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG262340.
HOGENOMHOG000232010.
HOVERGENHBG003456.
InParanoidQ13371.
OMAMQEYNML.
PhylomeDBQ13371.
TreeFamTF315179.

Gene expression databases

ArrayExpressQ13371.
BgeeQ13371.
CleanExHS_PDCL.
GenevestigatorQ13371.

Family and domain databases

Gene3D1.10.168.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR001200. Phosducin.
IPR023196. Phosducin_N_dom.
IPR024253. Phosducin_thioredoxin-like_dom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF02114. Phosducin. 1 hit.
[Graphical view]
PRINTSPR00677. PHOSDUCIN.
SUPFAMSSF52833. SSF52833. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPDCL. human.
GeneWikiPhosducin-like.
GenomeRNAi5082.
NextBio19608.
PROQ13371.
SOURCESearch...

Entry information

Entry namePHLP_HUMAN
AccessionPrimary (citable) accession number: Q13371
Secondary accession number(s): Q4VXB6 expand/collapse secondary AC list , Q96AF1, Q9UEW7, Q9UFL0, Q9UNX1, Q9UNX2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 17, 2003
Last modified: July 9, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM