cGMP-inhibited 3',5'-cyclic phosphodiesterase B

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Reviewed, UniProtKB/Swiss-Prot Q13370 (PDE3B_HUMAN)

Last modified July 7, 2009. Version 86. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase B
    EC=3.1.4.17
Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase B
      Short name=CGI-PDE B
    CGIPDE1
      Short name=CGIP1

Gene names

Name:

PDE3B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May play a role in fat metabolism.
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate.
Enzyme regulation	Inhibited by cGMP.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Abundant in adipose tissues.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

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Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Ligand	cGMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cAMP catabolic process Inferred from expression pattern. Source: UniProtKB cellular response to insulin stimulus Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cAMP-mediated signaling Inferred by curator. Source: UniProtKB negative regulation of cell adhesion mediated by integrin Inferred by curator. Source: UniProtKB negative regulation of lipid catabolic process Inferred from mutant phenotype. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB guanyl-nucleotide exchange factor complex Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Ref.1 Traceable author statement. Source: ProtInc protein kinase B binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1112

1112

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

PRO_0000198802

Regions

Transmembrane

88 – 108

Potential

Transmembrane

117 – 137

Potential

Transmembrane

152 – 172

Potential

Transmembrane

192 – 212

Potential

Transmembrane

220 – 240

Potential

Transmembrane

247 – 267

Potential

Compositional bias

1077 – 1080

Poly-Glu

Amino acid modifications

Modified residue

442

Phosphoserine Ref.4 Ref.5

Modified residue

444

Phosphothreonine Ref.4

Modified residue

625

Phosphothreonine By similarity

Natural variations

Natural variant

A → V: dbSNP rs1056584. Ref.3

VAR_031462

Experimental info

Sequence conflict

A → D in AAC50724. Ref.1

Sequence conflict

A → D in BAA09306. Ref.2

Secondary structure

1112

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q13370-1 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: 55451C3DA142EF6A
Show »

FASTA

1,112

124,333

        10         20         30         40         50         60 
MRRDERDAKA MRSLQPPDGA GSPPESLRNG YVKSCVSPLR QDPPRGFFFH LCRFCNVELR 

        70         80         90        100        110        120 
PPPASPQQPR RCSPFCRARL SLGALAAFVL ALLLGAEPES WAAGAAWLRT LLSVCSHSLS 

       130        140        150        160        170        180 
PLFSIACAFF FLTCFLTRTK RGPGPGRSCG SWWLLALPAC CYLGDFLVWQ WWSWPWGDGD 

       190        200        210        220        230        240 
AGSAAPHTPP EAAAGRLLLV LSCVGLLLTL AHPLRLRHCV LVLLLASFVW WVSFTSLGSL 

       250        260        270        280        290        300 
PSALRPLLSG LVGGAGCLLA LGLDHFFQIR EAPLHPRLSS AAEEKVPVIR PRRRSSCVSL 

       310        320        330        340        350        360 
GETAASYYGS CKIFRRPSLP CISREQMILW DWDLKQWYKP HYQNSGGGNG VDLSVLNEAR 

       370        380        390        400        410        420 
NMVSDLLTDP SLPPQVISSL RSISSLMGAF SGSCRPKINP LTPFPGFYPC SEIEDPAEKG 

       430        440        450        460        470        480 
DRKLNKGLNR NSLPTPQLRR SSGTSGLLPV EQSSRWDRNN GKRPHQEFGI SSQGCYLNGP 

       490        500        510        520        530        540 
FNSNLLTIPK QRSSSVSLTH HVGLRRAGVL SSLSPVNSSN HGPVSTGSLT NRSPIEFPDT 

       550        560        570        580        590        600 
ADFLNKPSVI LQRSLGNAPN TPDFYQQLRN SDSNLCNSCG HQMLKYVSTS ESDGTDCCSG 

       610        620        630        640        650        660 
KSGEEENIFS KESFKLMETQ QEEETEKKDS RKLFQEGDKW LTEEAQSEQQ TNIEQEVSLD 

       670        680        690        700        710        720 
LILVEEYDSL IEKMSNWNFP IFELVEKMGE KSGRILSQVM YTLFQDTGLL EIFKIPTQQF 

       730        740        750        760        770        780 
MNYFRALENG YRDIPYHNRI HATDVLHAVW YLTTRPVPGL QQIHNGCGTG NETDSDGRIN 

       790        800        810        820        830        840 
HGRIAYISSK SCSNPDESYG CLSSNIPALE LMALYVAAAM HDYDHPGRTN AFLVATNAPQ 

       850        860        870        880        890        900 
AVLYNDRSVL ENHHAASAWN LYLSRPEYNF LLHLDHVEFK RFRFLVIEAI LATDLKKHFD 

       910        920        930        940        950        960 
FLAEFNAKAN DVNSNGIEWS NENDRLLVCQ VCIKLADING PAKVRDLHLK WTEGIVNEFY 

       970        980        990       1000       1010       1020 
EQGDEEANLG LPISPFMDRS SPQLAKLQES FITHIVGPLC NSYDAAGLLP GQWLEAEEDN 

      1030       1040       1050       1060       1070       1080 
DTESGDDEDG EELDTEDEEM ENNLNPKPPR RKSRRRIFCQ LMHHLTENHK IWKEIVEEEE 

      1090       1100       1110 
KCKADGNKLQ VENSSLPQAD EIQVIEEADE EE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cDNA and gene encoding human PDE3B, the cGIP1 isoform of the human cyclic GMP-inhibited cyclic nucleotide phosphodiesterase family." Miki T., Taira M., Hockman S., Shimada F., Lieman J., Napolitano M., Ward D., Taira M., Makino H., Manganiello V.C. Genomics 36:476-485(1996) [PubMed: 8884271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Adipose tissue.
[2]	"Differential expression of cGMP-inhibited cyclic nucleotide phosphodiesterases in human hepatoma cell lines." Murata T., Taira M., Manganiello V.C. FEBS Lett. 390:29-33(1996) [PubMed: 8706823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular cloning and chromosomal assignment of the human homologue of the rat cGMP-inhibited phosphodiesterase 1 (PDE3A) -- a gene involved in fat metabolism located at 11p15.1." Loebbert R.W., Winterpacht A., Seipel B., Zabel B.U. Genomics 37:211-218(1996) [PubMed: 8921398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-87.
[4]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442 AND THR-444, MASS SPECTROMETRY.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U38178 Genomic DNA. Translation: AAC50724.1.
D50640 Genomic DNA. Translation: BAA09306.1.
X95520 mRNA. Translation: CAA64774.1.

IPI

IPI00012843.

PIR

S70522.

RefSeq

NP_000913.2.

UniGene

Hs.445711

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SO2	X-ray	2.40	A/B/C/D	654-1073	[»]
1SOJ	X-ray	2.90	A/B/C/D/E/F/G/H/I/J/K/L	654-1073	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q13370.

Proteomic databases

PRIDE

Q13370.

Genome annotation databases

Ensembl

ENSG00000152270. Homo sapiens. [Contig view]

GeneID

5140.

KEGG

hsa:5140.

UCSC

uc001mln.1. human.

Organism-specific databases

GeneCards

GC11P014621.

HGNC

HGNC:8779. PDE3B.

HPA

CAB009497.

MIM

602047. gene.

PharmGKB

PA33127.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q13370.

HOVERGEN

Q13370.

OMA

Q13370. LRNSDSN.

Enzyme and pathway databases

BRENDA

3.1.4.17. 247.

Pathway_Interaction_DB

pi3kcibpathway. Class IB PI3K non-lipid kinase events.

Reactome

REACT_1123. Inhibition of HSL.
REACT_498. Signaling by Insulin receptor.

Gene expression databases

ArrayExpress

Q13370.

Bgee

Q13370.

CleanEx

HS_PDE3B.

GermOnline

ENSG00000152270. Homo sapiens.

Family and domain databases

InterPro

IPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
[Graphical view]

Pfam

PF00233. PDEase_I. 1 hit.
[Graphical view]

SMART

SM00471. HDc. 1 hit.
[Graphical view]

PROSITE

PS00126. PDEASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

19820.

SOURCE

Search...

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Entry information

Entry name

PDE3B_HUMAN

Accession

Primary (citable) accession number: Q13370
Secondary accession number(s): O00639, Q14408

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 3, 2007
Last modified:	July 7, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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