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Reviewed, UniProtKB/Swiss-Prot Q13370 (PDE3B_HUMAN)

Last modified July 7, 2009. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase B
    EC=3.1.4.17
Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase B
      Short name=CGI-PDE B
    CGIPDE1
      Short name=CGIP1
Gene names
Name: PDE3B
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1112 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May play a role in fat metabolism.

Catalytic activity

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Enzyme regulation

Inhibited by cGMP.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Abundant in adipose tissues.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11121112cGMP-inhibited 3',5'-cyclic phosphodiesterase B
PRO_0000198802

Regions

Transmembrane88 – 10821 Potential
Transmembrane117 – 13721 Potential
Transmembrane152 – 17221 Potential
Transmembrane192 – 21221 Potential
Transmembrane220 – 24021 Potential
Transmembrane247 – 26721 Potential
Compositional bias1077 – 10804Poly-Glu

Amino acid modifications

Modified residue4421Phosphoserine Ref.4 Ref.5
Modified residue4441Phosphothreonine Ref.4
Modified residue6251Phosphothreonine By similarity

Natural variations

Natural variant871A → V: dbSNP rs1056584. Ref.3
VAR_031462

Experimental info

Sequence conflict841A → D in AAC50724. Ref.1
Sequence conflict841A → D in BAA09306. Ref.2

Secondary structure

................................................... 1112
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q13370-1 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: 55451C3DA142EF6A

FASTA1,112124,333
        10         20         30         40         50         60 
MRRDERDAKA MRSLQPPDGA GSPPESLRNG YVKSCVSPLR QDPPRGFFFH LCRFCNVELR 

        70         80         90        100        110        120 
PPPASPQQPR RCSPFCRARL SLGALAAFVL ALLLGAEPES WAAGAAWLRT LLSVCSHSLS 

       130        140        150        160        170        180 
PLFSIACAFF FLTCFLTRTK RGPGPGRSCG SWWLLALPAC CYLGDFLVWQ WWSWPWGDGD 

       190        200        210        220        230        240 
AGSAAPHTPP EAAAGRLLLV LSCVGLLLTL AHPLRLRHCV LVLLLASFVW WVSFTSLGSL 

       250        260        270        280        290        300 
PSALRPLLSG LVGGAGCLLA LGLDHFFQIR EAPLHPRLSS AAEEKVPVIR PRRRSSCVSL 

       310        320        330        340        350        360 
GETAASYYGS CKIFRRPSLP CISREQMILW DWDLKQWYKP HYQNSGGGNG VDLSVLNEAR 

       370        380        390        400        410        420 
NMVSDLLTDP SLPPQVISSL RSISSLMGAF SGSCRPKINP LTPFPGFYPC SEIEDPAEKG 

       430        440        450        460        470        480 
DRKLNKGLNR NSLPTPQLRR SSGTSGLLPV EQSSRWDRNN GKRPHQEFGI SSQGCYLNGP 

       490        500        510        520        530        540 
FNSNLLTIPK QRSSSVSLTH HVGLRRAGVL SSLSPVNSSN HGPVSTGSLT NRSPIEFPDT 

       550        560        570        580        590        600 
ADFLNKPSVI LQRSLGNAPN TPDFYQQLRN SDSNLCNSCG HQMLKYVSTS ESDGTDCCSG 

       610        620        630        640        650        660 
KSGEEENIFS KESFKLMETQ QEEETEKKDS RKLFQEGDKW LTEEAQSEQQ TNIEQEVSLD 

       670        680        690        700        710        720 
LILVEEYDSL IEKMSNWNFP IFELVEKMGE KSGRILSQVM YTLFQDTGLL EIFKIPTQQF 

       730        740        750        760        770        780 
MNYFRALENG YRDIPYHNRI HATDVLHAVW YLTTRPVPGL QQIHNGCGTG NETDSDGRIN 

       790        800        810        820        830        840 
HGRIAYISSK SCSNPDESYG CLSSNIPALE LMALYVAAAM HDYDHPGRTN AFLVATNAPQ 

       850        860        870        880        890        900 
AVLYNDRSVL ENHHAASAWN LYLSRPEYNF LLHLDHVEFK RFRFLVIEAI LATDLKKHFD 

       910        920        930        940        950        960 
FLAEFNAKAN DVNSNGIEWS NENDRLLVCQ VCIKLADING PAKVRDLHLK WTEGIVNEFY 

       970        980        990       1000       1010       1020 
EQGDEEANLG LPISPFMDRS SPQLAKLQES FITHIVGPLC NSYDAAGLLP GQWLEAEEDN 

      1030       1040       1050       1060       1070       1080 
DTESGDDEDG EELDTEDEEM ENNLNPKPPR RKSRRRIFCQ LMHHLTENHK IWKEIVEEEE 

      1090       1100       1110 
KCKADGNKLQ VENSSLPQAD EIQVIEEADE EE 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the cDNA and gene encoding human PDE3B, the cGIP1 isoform of the human cyclic GMP-inhibited cyclic nucleotide phosphodiesterase family."
Miki T., Taira M., Hockman S., Shimada F., Lieman J., Napolitano M., Ward D., Taira M., Makino H., Manganiello V.C.
Genomics 36:476-485(1996) [PubMed: 8884271] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Adipose tissue.
[2]"Differential expression of cGMP-inhibited cyclic nucleotide phosphodiesterases in human hepatoma cell lines."
Murata T., Taira M., Manganiello V.C.
FEBS Lett. 390:29-33(1996) [PubMed: 8706823] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Molecular cloning and chromosomal assignment of the human homologue of the rat cGMP-inhibited phosphodiesterase 1 (PDE3A) -- a gene involved in fat metabolism located at 11p15.1."
Loebbert R.W., Winterpacht A., Seipel B., Zabel B.U.
Genomics 37:211-218(1996) [PubMed: 8921398] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-87.
[4]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442 AND THR-444, MASS SPECTROMETRY.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

U38178 Genomic DNA. Translation: AAC50724.1.
D50640 Genomic DNA. Translation: BAA09306.1.
X95520 mRNA. Translation: CAA64774.1.
IPIIPI00012843.
PIRS70522.
RefSeqNP_000913.2.
UniGeneHs.445711

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1SO2X-ray2.40A/B/C/D654-1073[»]
1SOJX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L654-1073[»]
ModBaseSearch...

PTM databases

PhosphoSiteQ13370.

Proteomic databases

PRIDEQ13370.

Genome annotation databases

EnsemblENSG00000152270. Homo sapiens. [Contig view]
GeneID5140.
KEGGhsa:5140.
UCSCuc001mln.1. human.

Organism-specific databases

GeneCardsGC11P014621.
HGNCHGNC:8779. PDE3B.
HPACAB009497.
MIM602047. gene.
PharmGKBPA33127.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ13370.
HOVERGENQ13370.
OMAQ13370. LRNSDSN.

Enzyme and pathway databases

BRENDA3.1.4.17. 247.
Pathway_Interaction_DBpi3kcibpathway. Class IB PI3K non-lipid kinase events.
ReactomeREACT_1123. Inhibition of HSL.
REACT_498. Signaling by Insulin receptor.

Gene expression databases

ArrayExpressQ13370.
BgeeQ13370.
CleanExHS_PDE3B.
GermOnlineENSG00000152270. Homo sapiens.

Family and domain databases

InterProIPR003607. Met-dep_phosphohydro_HD.
IPR002073. PDEase.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio19820.
SOURCESearch...

Entry information

Entry namePDE3B_HUMAN
AccessionPrimary (citable) accession number: Q13370
Secondary accession number(s): O00639, Q14408
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 3, 2007
Last modified: July 7, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents